• Title, Summary, Keyword: 암세포 증식억제 효과

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Antiproliferative Effect of Bacillus subtilis Fermented Soy Milk in AGS Human Gastric Adenocarcinoma Cells (Bacillus subtilis 발효두유의 AGS 인체 위암세포 증식억제 효과)

  • Seo, Hae-Ree;Kim, Ji-Young;Bae, Geun-Ho;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.5
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    • pp.644-648
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    • 2009
  • Antiproliferative effects of soy milk fermented with Bacillus subtilis from chungkukjang was studied in AGS human gastric adenocarcinoma cells. The fermented soy milk by B. subtilis (B. subtilis-F-SM) exhibited 82% growth inhibitory effect at 2 mg/mL concentration, while non-fermented soy milk (Non-F-SM) showed 68%. B. subtilis-F-SM treated AGS cells induced more apoptotic bodies than the Non-F-SM treated cells. In mRNA expressions, B. subtilis-F-SM showed decreased expression of anti-apoptotic bcl-2 and increased expression of pro-apoptotic bax. The expressions of tumor suppressor genes of p53 and p21 were also increased. These results suggest that fermented soy milk by B. subtilis exhibited higher antiproliferative activities compared with non-fermented soy milk.

Apoptotic Effects of Curcumin and EGCG via Akt-p53 Signaling Pathway in HCT116 Colon Cancer Cells (HCT116 대장암 세포에서 Akt-p53 신호경로를 통한 커큐민과 EGCG의 apoptosis 효과)

  • Park, Song-Yi;Lee, Sol-Hwa;Park, Ock-Jin;Kim, Young-Min
    • Journal of Life Science
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    • v.21 no.1
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    • pp.89-95
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    • 2011
  • p53 is tumor suppressor gene that regulates apoptosis such as caspase-dependent and p21-mediated signaling pathways. PI3K/Akt is known to be over-activated in cancer cells. Akt activates many survival-related signals such as mTOR and COX-2. Inactivation of Akt would result in non-inhibition of p53 as well as induced apoptosis. In this study, we showed that curcumin and EGCG activate p53 via inhibition of the Akt signaling pathway. Treatments using curcumin and EGCG in different concentrations for 24 hr and 48 hr inhibited proliferation of HCT116 colon cancer cells and increased apoptotic cell death. Also, our data showed that curcumin and EGCG increased the p53 expression and decreased the p-Akt. Treatment of LY294002 (Akt inhibitor) resulted in decreased cell proliferation of cancer cells, while LY294002 treated with curcumin or EGCG showed a greater decrease of cell proliferation. In addition, inhibition of Akt induced p53 activation in HCT116 colon cancer cells. These results suggest that curcumin and EGCG induce apoptosis by inhibiting Akt and increase p53 in HCT116 colon cancer cells.

Growth Inhibitory Effect of Irradiated Green Tea Polyphenol Addition in Cosmetic Composition (녹차 폴리페놀을 첨가한 화장품의 암 세포증식억제 효과)

  • Park, Tae-Soon;Lee, Jin-Young;Park, Gun-Hye;Hyun, Sok-Jun;Lee, Jin-Tae;Cho, Young-Je;Kim, Young-Sun;An, Bong-Jeun
    • Applied Biological Chemistry
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    • v.50 no.3
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    • pp.217-223
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    • 2007
  • Cosmetic products including toner and essence were manufactured to evaluate the effect of green tea polyphenols. In addition, irradiation was applied to remove an undesirable color of green tea polyphenol(GTP), which may cause a problem in marketing. The growth inhibition rates of GTP, PT, and PE on all cell lines were shown to be over 80% at 500 ppm concentration. Especially the growth inhibition rates of GTP, PT, and PE on human melanoma(G361) cells were shown to be over 80% at only 100 ppm concentration. Results indicate that the addition of irradiated green tea polyphenol may be effective in the manufacturing of functional cosmetics including toner and essence with various anti-cancer activities.

Antioxidant, Tyrosinase Inhibitory, and Anti-proliferative Activities of Gochujang Added with Cheonggukjang Powder Made from Sword Bean (작두콩 청국장 첨가 고추장의 항산화, tyrosinase 저해 및 암세포 증식 억제 효과)

  • Chang, Moon-Ik;Kim, Jae-Young;Kim, Un-Sung;Baek, Seung-Hwa
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.221-226
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    • 2013
  • This study aimed to examine the antioxidant, tyrosinase inhibitory, and anti-proliferative activities (A549, G361, HT-29, and MDA-MB-231) of fermented gochujang (made from sword bean cheonggukjang powder (SBC) for 90 days. Gochujang was prepared by adding 0 (SBC 0), 2 (SBC 2), 5 (SBC 5), 8 (SBC 8) and 10% (SBC 10) levels with SBC, and all experiments were measured at diluted levels of 20, 50 and 100 times. The antioxidant activity and tyrosinase inhibitory effect demonstrated that SBC 10 increased approximately 1.2 and 1.1 times compared with SBC 0, respectively, at diluted levels of 50 and 100 times. The anti-proliferative effects of A549, G361, and HT-29 presented that SBC 10 were 2.8, 1.1, and 8.9 times higher compared with SBC 0, respectively, at diluted levels of 50, 20, and 100 times. In the case of MDA-MB-231, SBC 10 was 3.7 times higher compared with SBC 5 at diluted level of 20 times. As a result, we confirmed that SBC gochujang was improved for physiological activities and anti-proliferative effects.

The Effects on Antimicrobial and Cytotoxicity of Brassica oleracea L. Fractions. (적채 분획물의 항균, 암세포 증식 억제효과)

  • 박윤자;전광혜;김선희;배송자
    • Journal of Life Science
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    • v.14 no.4
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    • pp.567-572
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    • 2004
  • This study was carried out to investigate the affects on antimicrobial and cytotoxicity of red cabbage (Brassica oleracea L.,BO). In the paper disc test, antimicrobial activity of BO fractions was increased in proportion to its concentration. Among the various solvent fractions of methanol extract (BOM) of BO, the ethylacetate partition layer (BOMEA) showed the strongest antimicrobial activity We also determined the cytotoxicity and Quinone Reductase induced effect of BO extract and fractions on human cancer cells. The cytotoxicity of BO fractions on HepG2, HeLa and MCF-7 cells was evaluated by MTT assay. The BOMEE and BOMEA showed strong cytotoxic effects on all cancer cell lines we used. The quinone reductase induced effect of BO fractions on HepG2 cells, the hexane partition layer (BOMH) at a dose of 200 $\mu{g}$/ml was 2.88 times more effective compared to the control values of 1.0.

Inhibitory Effect of Low-molecularized Polymannuronate on Proliferation and DNA Synthesis of Human Colon Cancer Cells (저분자 polymannuronate의 인체 대장암세포 증식 및 DNA 합성 저해 효과)

  • Kim In-Hye;Nam Taek-Jeong
    • Journal of Life Science
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    • v.15 no.6
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    • pp.857-862
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    • 2005
  • This study investigated the proliferation and DNA synthesis inhibitory effect of concentrations ($0.01\%$, $0.1\%$, $0.25\%$, $0.5\%$) when added whole molecular-, 40 kDa-, or 10 kDa polymannuronate on human colon cancer cells, HT-29, DLD-1, and WiDr, in vitro. In order to determine the proliferation inhibitory effect of low-molecularized polymannuronate, the treatment of whole molecular-, 40 kDa-, 10 kDa-, polymannuronate ($0.25\%$) to the HT-29 cancer cells inhibited proliferation of cancer cells by $41\%$, $69.1\%$, and $75.6\%$, respectively. DLD-1 cancer cell was not relation of molecular weight and concentration. WiDr cancer cell depend on concentration without molecular weight. In addition, whole molecular-, 40 kDa-, 10 kDa poly mannuronate ($0.25\%$) significantly inhibited DNA synthesis of HT-29 cancer .cells by $78\%$, $58\%$, and $56\%$, respectively. And morphological changes not found under microscope by polymannuronate. Therefore polymannuronate would be helpful to colon cancer treatment as well as cancer prevention and this study would be the basic source for further research of polymannuronate.

The Effects on Antimicrobial and Cytotoxicity of Solanum Iyratum Fractions (배풍등 분획물의 항균 및 암세포 증식 억제효과)

  • Shin Mi-Ok
    • Journal of Life Science
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    • v.15 no.6
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    • pp.948-954
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    • 2005
  • In this study, we investigated antimicrobial and cytotoxicity effects to each fraction extracted from Solanum lyratum (SL), which were extracted methanol (SLM) and then the extract was fractionated into five different types : hexane (SLMH), ethyl ether (SLMEE), ethylacetate (SLMEA), butanol (SLMB) and aqueous (SLMA). The antimicrobial activity was analyzed by the paper disc method. Among the various solvent fractions, SLMEA showed the strongest antimicrobial activies. The cytotoxicity of SL fractions on HeLa, MCF-7, HT-29 and HepG2 cells was evaluated by MTT assay. Among various partition layers, SLMEE showed the strongest cytotoxic effects to all cancer cell lines. We also observed that quinone reductase (QR) was induced by all fraction layers of SL to HepG2 cells. Since the QR-induced effects of SLMEE on HepG2 cells at $160{\mu}g/ml$ concentration showed 2.1 when compared with a control value of 1.0, inducer of QR for cancer protection may be contained in this fraction.

Growth Inhibition Effects of Cancer Cell Lines by Gloiopeltis furcata Fractions in Vitro (불등가사리 분획물의 암세포 성장 억제 효과)

  • Park, Soung-Young;Jung, Bok-Mi;Choi, Yung-Hyun;Bae, Song-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.6
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    • pp.771-775
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    • 2005
  • The growth inhibitory effects on human cancer cell lines provide useful information regarding critical cellular targets. Reports on cytotoxicity of Gloiopeltis furcata (GF) to human cancer cell lines are conflicting. This study was performed to investigate the effects of cytotoxicity and quinone reductase activity of Gloiopeltis furcata on the human cancer cells. The four partition layers of methanol extracts (GFM) which are hexane (GFMH), methanol (GFMM), butanol (GFMB) and aquous (GFMA) were screened for their cytotoxic effects on HepG2, HeLa, MCF-7, HT-29, and normal liver cell lines. The GFMM showed the strongest growth inhibition effect on all cell lines we used. the GFMM showed the highest induction activity of quinone reductase on HepG2 cells among the other partition layers.

Growth-inhibitory Effect of the Extract of Porphyran-Chungkookjang on Cancer Cell (Porphyran-청국장 추출물의 암세포 성장 억제효과)

  • Min, Hyun-Kyeng;Kim, Hyo-Ju;Chang, Hae-Choon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.7
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    • pp.826-833
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    • 2008
  • The effects of porphyran-chungkookjang on cytotoxicity of human normal cell line (BJ) and human cancer cell lines (AGS and HT-29) were examined. Porphyran, which was prepared from laver (Porphyra yezoensis), decreased the viability of the cancer cells, however, it did not affect the viability of normal cells. Porphyranchungkookjang was prepared by the addition of 5% (w/w) porphyran into chungkookjang which was fermented by starter, Bacillus subtilis DJI. The cytotoxicity effects of the chungkookjang and porphyran-chungkookjang were evaluated with MTT assay. The methanol and the water extract of porphyran-chungkookjang at 1.0 mg/mL showed $23{\sim}38%$ decreases in proliferation of cancer cells (AGS and HT-29). However, the methanol and the water extracts of porphyran-chungkookjang did not inhibit the growth of normal cell. Moreover, the methanol extract of porphryan-chungkookjang at 1.0 mg/mL showed $1.2{\sim}1.5$ fold higher anticancer effects than that of the chungkookjang.

Fatty Acid Composition and Antiproliferative Activity of Extracts from Euphorbia Supina (애기땅빈대 추출물의 지방산 조성 및 인체 암세포 증식 억제 효과)

  • Choi, Hyang Mi;Lim, Sun Young
    • Journal of Life Science
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    • v.24 no.1
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    • pp.74-80
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    • 2014
  • The objective of this study was to determine the fatty acid composition and the antiproliferative effect of extracts and fractions from Euphorbia supina. With regards the fatty acid composition, the percentages of 18:3n-3 in acetone/methylene chloride (A+M) and methanol (MeOH) extracts were 53.4 and 42.1%, respectively. Among the fractions, an 85% aqueous methanol (85% aq. MeOH) fraction contained the highest percentage of 18:3n-3. Treatments with crude extracts and fractions significantly inhibited the growth of HT-29 and AGS human cancer cell lines (p<0.05). The A+M extract showed a higher inhibitory effect on the growth of both cancer cells compared to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions exerted a greater inhibitory effect on the proliferation of both types of cancer cells. Our results suggest that 85% aq. MeOH and n-hexane fractions exert potent inhibitory effects on the proliferation of human cancer cells.