• Title, Summary, Keyword: 애기장대

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Molecular Cloning and Characterization of Outer Envelope Membrane Protein from Salicornia herbacea (퉁퉁마디로부터 색소체 외막 단백질 유전자의 분리 및 발현분석)

  • Ermawati Netty;Cha, Joon-Yung;Liang, Yingshi;Jung, Min-Hee;Shin, Dongjin;Lee, Byung-Hyun;Lee, Kon-Ho;Son, Daeyoung
    • Journal of Plant Biotechnology
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    • v.31 no.4
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    • pp.273-278
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    • 2004
  • Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na$^{+}$ sensitivity of yeast mutant (cnb$\Delta$) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.ant salt stress.

Comparison and analysis of multiple testing methods for microarray gene expression data (유전자 발현 데이터에 대한 다중검정법 비교 및 분석)

  • Seo, Sumin;Kim, Tae Houn;Kim, Jaehee
    • Journal of the Korean Data and Information Science Society
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    • v.25 no.5
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    • pp.971-986
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    • 2014
  • When thousands of hypotheses are tested simultaneously, the probability of rejecting any true hypotheses increases, and large multiplicity problems are generated. To solve these problems, researchers have proposed different approaches to multiple testing methods, considering family-wise error rate (FWER), false discovery rate (FDR) or false nondiscovery rate (FNR) as a type I error and some test statistics. In this article, we discuss Bonferroni (1960), Holm (1979), Benjamini and Hochberg (1995) and Benjamini and Yekutieli (2001) procedures based on T statistics, modified T statistics or local-pooled-error (LPE) statistics. We also consider Sun and Cai (2007) procedure based on Z statistics. These procedures are compared in the simulation and applied to Arabidopsis microarray gene expression data to identify differentially expressed genes.

Studies on nickel uptake in transgenic Arabidopsis thaliana introduced with TgMTP1 gene encoding metal tolerance protein (TgMTP1 과발현 애기장대에서 Nickel 흡수 연구)

  • Kim, Donggiun
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.409-413
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    • 2015
  • To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both $TgMTP_1$ (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that $TgMTP_1$-overexpressing plants may be useful for phytoremediation of soil.

AtMYB7 Acts as a repressor of lignin biosynthesis in Arabidopsis (애기장대 MYB7 유전자의 리그닌 생합성 억제 조절)

  • Kim, Won-Chan
    • Journal of Applied Biological Chemistry
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    • v.59 no.3
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    • pp.215-220
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    • 2016
  • Abstract Secondary cell wall is the most abundant biomass produced by plants. Plant secondary cell wall is composed of a complex mixture of cellulose, hemicellulose, and lignin. Lignin, a phenolic polymer that hinders the degradation of cell wall polysaccharides to simple sugars destined for fermentation to bio-ethanol. Cell wall biosynthesis pathway-specific biomass engineering offers an attractive 'genetic pretreatment' strategy to improve bioenergy feedstock. Recently, we found a transcription factor, MYB7, which is a transcriptional switch that may turns off the genes necessary for lignin biosynthesis. To gain insights into MYB7 mediated transcriptional regulation, we first established a dominant suppression system in Arabidopsis by expressing MYB7-SRDX. Then we used a transient transcriptional activation assay to confirm that MYB7 suppress the transcription of the lignin biosynthetic gene. Taken together, we conclude that MYB7 function as a repressor of the genes involved in the lignin biosynthesis.

Studies on the tolerance of Halophyte Arabis stelleri under heavy metals and Salt stress condition (염생식물 섬갯장대(Arabis stelleri var. japonica)의 중금속 및 고염 농도 스트레스 상태에서 내성 연구)

  • Kim, Donggiun
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.4
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    • pp.373-378
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    • 2019
  • In the marine area, the salt concentration in the soil increases, and the inland heavy metal pollution increases the damage of plants. In the inland industrial development area, researches on the genetic resources of plants together with the heavy metal accumulation of Co, Ni, Zn, and so on are required. Both of these problems have caused scientists to work hard to find plants that are likely to cause stress in plant roots. In this study, seeds of Arabis stelleri var. japonica collected near the shore were used for germination. The growth and development and tolerance of both Arabis and Arabidopsis seeds were investigated under laboratory culture conditions. As a result, Arabis showed resistance about 3 times in 250 mM nickle and cobalt, and more than 4 times in 1 mM zinc when compared to Arabidopsis. The tolerance of Arabis to Na salts increased by 20% or more at 50 mM concentration and Arabis was resistant to heavy metals and salt concentration. The accumulation of Na ions in the body was measured as a preparation for studying the intracellular mechanism. As a result, it showed a further decrease in resistance to ground water roots. It is considered that the activity of the exporting gene is important rather than the mechanism of accumulation.

Pseudosymptom Analysis Induced by Geminivirus L4 Gene in Transgenic Arabidopsis (Geminivirus L4 유전자 도입에 따른 형질전환 애기장대의 감염유사증상 분석)

  • Lee, Suk-Chan;Lee, Kyu-Bae;Park, Jong-Bum
    • Journal of Plant Biotechnology
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    • v.30 no.2
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    • pp.129-133
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    • 2003
  • The factors for symptom development caused by Beat curly top virus(BCTV) have been analyzed by using a molecular genetic approach based on expressing BCTV encoded proteins in transgenic plants. BCTV open reading frame (ORF) L4 expression in transgenic Arabidopsis resulted in abnormal plant development and the production of callus inflorescence stems and bumpy trichomes, confiming that this gene alone is a primary symptom determinant. The L4 gene expression by northern hybridization in transgenic plants and a range of phenotypes were analyzed.

Determination Times for Induction of Adventitious Shoots, Roots, Trichomes, and Calli from Segments of Arabidopsis thaliana by NAA and BA (애기장대(Arabidopsis thaliana)잎 절편에서 NAA와 BA에 의한 신초, 부정근, 모용 및 캘러스 형성 결정 시기)

  • Kim, Song-Lim;Han, Tae-Jin
    • Journal of Plant Biotechnology
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    • v.30 no.2
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    • pp.161-165
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    • 2003
  • The effect of NAA and Benzyladenine(BA) for determination times on the formation of adventitious shoots, roots trichmoes and calli in MS basal medium was investigated in leaf segments from ecotype 'Nosses' of Arabidopsis thalliana. Adventitious shoots, roots, trichomes and calli were formed fromed from leaf segments in a wede range of NAA and BA. The optimal combination of hormones for adventitious shoots formation, 20mg/L NAA for trichome formation, 100mg/L for callus formation. Inductive times for formation of adventitious shoots, roots, trichomes and calli were determined at 14, 4, 6 and 18 days respectively by periodical transfer of leaf segments from hormines containing media to hormone free medium.

Phytochromes are Involved in the Regulation of Growth and the Gravitropic Response via Ethylene Production in Hypocotyl of Arabidopsis (애기장대의 하배축에서 피토크롬이 생장과 굴중성 반응에 미치는 영향)

  • Lee, Sang Seung;Kim, Soon Young
    • Journal of Life Science
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    • v.28 no.1
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    • pp.9-16
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    • 2018
  • Light is essential to the growth and development of plants, and it is perceived by phytochromes, which are one of the photoreceptors that regulate physiological responses in plants. Ethylene regulates the dormancy, senescence, growth, and development of organs in plants. This research focused on the interaction of phytochromes and ethylene to control hypocotyl growth and gravitropism using phytochrome mutants of Arabidopsis, phyA, phyB, and phyAB, under three light conditions: red (R) light, farred (FR) light, and white light. The mutant phyAB exhibited the most stimulation of gravitropic response of all three phytochrome mutants and wild type (WT) in all three light conditions. Moreover, phyB in the R light condition showed more negative gravitropism than phyA. However, phyB in the FR light condition showed less curvature than phyA. The hypocotyl growth pattern was similar to the gravitropic response in several light conditions. To explain the mechanism of the regulation of gravitropic response and growth, we measured the ethylene production and activities of in vitro ACS and ACO. Ethylene production was reduced in all the mutants grown in white light in comparison to the WT. Ethylene production increased in the phyA grown in R light and phyB grown in FR light in comparison to the other mutants. The ACS activity coincided with the ethylene production in the phyA and the phyB grown in R light and FR light, respectively. These results suggest that the Pfr form of phyB in R light and the Pr form of phyA in FR light increased ethylene production via increasing ACS activity.

Expression Study on the Scaffold Gene of CRL4 Complex in Rice (Oryza sativa L.) (벼에 존재하는 CRL4 복합체 scaffold 유전자의 발현 양상에 대한 연구)

  • Bae, Yoowon;Kim, Hani;Kim, Sang-Hoon;Lee, Jae-Hoon
    • Journal of Life Science
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    • v.28 no.10
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    • pp.1132-1139
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    • 2018
  • The stability of diverse cellular proteins in eukaryotes is regulated via ubiquitination. Moreover, E3 ligase plays a crucial role in determining substrate specificity and transfers ubiquitins into the substrates during the ubiquitination process. As a type of multi-subunit E3 ligase, cullin4 (CUL4)-based E3 ligase (CRL4) complex is involved in a variety of cellular processes, such as hormonal and stress responses in plants. In spite of several reports on the versatile roles of CRL4 in various signalings in Arabidopsis, CRL4's function in rice has been poorly known. To learn about CRL4-mediated cellular processes in rice in more detail, OsCUL4 that exhibits the highest homology with Arabidopsis CUL4 was isolated, and its expression patterns in various tissues and in response to plant hormones and abiotic stresses were monitored. Exogenous application of ABA or cytokinin increased the transcript levels of the OsCUL4 gene. Moreover, OsCUL4 was significantly upregulated in response to drought and salt stresses. These findings imply that OsCUL4 may be functionally related to ABA- and/or cytokinin-mediated cellular responses. OsCUL4 directly interacted with OsDDB1, an adaptor protein of CRL4, indicating that OsCUL4 can act as a scaffold protein of CRL4. An expression study on the OsCUL4 gene from this report could be used as a starting point to elucidate cellular responses in which a CRL4-mediated ubiquitination process is involved in rice.

Isolation of cDNA Encoding Low Temperature-inducible L-asparaginase from Soybean (Glycin max) (저온 스트레스에 발현이 유도되는 콩의 L-asparaginase 유전자의 분리)

  • Park, Seong-Whan;Kim, Kee-Young;Chen, Liang;Lee, Jai-Heon
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.99-104
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    • 2002
  • Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.