• Title, Summary, Keyword: 인산화반응

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인슐린의 신호전달 기전 : Transcription Factor AP-1 의 역활

  • 김성진
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.17-21
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    • 1995
  • 대부분의 인슐린의 작용들은 인슐린 수용체를 통하여 이루어진다. 인슐린이 수용체에 결합하면, 수용체 고유의 tyrosine kinase 효소활성의 증가를 유발시키며, 결과적으로 세포내에 존재하는 기질 단백질, IRS-1, 의 tyrosine 잔기의 인산화를 증가시키게 된다. 이후, 여러 형태의 serine / threonine protein kinase 의 연속적인 활성화가 일어난다. 이들에 부가해서, 인슐린의 효자는 세포핵 내에까지 전달되어 유전자 발현의 조절과 같은 세포핵 고유의 활동에도 관여한다. 현재, 세포막에서 시작된 인슐린의 신호들이 세포핵까지 전달되는 정확한 기전에 대해서는 알려진 바 없지만, 최근의 연구에 의하면 MAP Kinase 와 S6 Kinase 그리고 Transcription Factor AP-1의 중요성이 제시되고 있다. 특히 유전자 조절 기전에는 핵단백질인 transcription factor의 인산화 반응이 큰 역할을 한다고 보고되고 있는바, 본 연구에서 AP-1. transcription factor 의 인산화 반응이 인슐린의 신호전달계에 미치는 역할에 대하여 고찰하였다. 요약하면, AP-1 transcription factor의 구성원인 c-Jun, c-Fos 그리고 Fos 관련 단백질들의 인산화가 인슐린에 의해 증가되며, 동시에 그들의. DNA-binding activity 와 유전자 발현의 활성이 증가됨을 밝힘으로써, AP-1 transcription factor의 인산화 반응이 인슐린의 핵 내에서의 작용기전에 중요한 역할을 함이 제시되고 있다. 또한 AP-1 의 인산화 반응에 관여하는 세포핵 protein kinase로서 Casein Kinase II 의 중요성이 밝혀졌다.

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Effects of Caffeic Acid on the Rates of Maillard Reaction (마이얄반응속도에 미치는 카페인산의 영향)

  • Son, Jong-Youn;Ahn, Myung-Soo
    • Korean journal of food and cookery science
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    • v.10 no.2
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    • pp.161-165
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    • 1994
  • It was aimed to investigate the effects of caffeic acid on the rates of Maillard reaction. The rates of browning reaction increased as the browning temperature increased. The color intensity of the browning mixtures indicated to depend on the amino acid rather than reducing sugar. Also, the color intensity of the browning mixtures increased more rapidly in the presence of caffeic acid. The increase in color intensity seemed to depend mainly to the polymerization of o-quinones formed from caffeic acid. The caffeic acid, furthermore, appeared to enhance the color intensity of the browning mixtures through the interaction with amino acid, especially methionine and phenylalanine. The activation ener-gies of the browning reaction without caffeic acid were 108∼130 J/mol, and Q10 values were 2.6∼3.2. The activation energies and Q10 values of browning mixtures decreased in the presence of CA. The activation energies of the browning mixtures with caffeic acid were 90∼101J/mol, and Q$\_$10/ values were 2.0∼2.6.

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Effects of Annealing Conditions of Corn Starch Slurry on the Formation of Phosphorylated Cross-linked Resistant Starch (옥수수 전분유의 Annealing 조건이 인산가교 저항 전분의 형성에 미치는 영향)

  • Bae, Chun-Ho;Park, Heui-Dong
    • Korean Journal of Food Preservation
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    • v.19 no.2
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    • pp.216-222
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    • 2012
  • The optimum annealing conditions of corn starch slurry were studied for RS4 type resistant starch production by phosphorylated cross-linking. When a corn starch slurry was cross-linked by using phosphate salts (STMP/STPP mixture) in the presence of 0.9%, 1.2% and 1.5% NaOH/st.ds, a high concentration of NaOH resulted in a rapid increase of the RS contents at the early reaction stage. However, similar RS contents were obtained after 12 h of cross-linking regardless of NaOH concentrations. The annealing treatment was conducted under various conditions such as pH between 2-10, temperature $40-60^{\circ}C$, time 0-14 h followed by phosphorylated cross-linking. The lower slurry pH was for the annealing treatment, the higher RS contents were obtained after cross-linking. When the slurry annealed for various period of time and temperature, a maximal amount of RS was formed after 2 h of annealing at $50^{\circ}C$ of annealing temperature of the starch slurry (pH 2.0). Therefore, an optimal annealing conditions at pH 2.0 and $50^{\circ}C$ for 2 h were proposed under the cross-linking conditions of sodium sulfate 10%/st.ds, NaOH 1.2%/st.ds and 12 h of the reaction time. The RS contents were linearly increased with the increase of phosphate salt addition. The RS4 prepared under the optimal conditions contained RS 72.3% and its phosphorus content was 0.36%/st.ds, which was below the limit (0.4%/st.ds) of modified starch by Korea Food Additives Code.

Phosphorylation of silk fibroin and its properties (견 피브로인의 인산화와 그 특성)

  • 문장희;김정호;배도규;신봉섭
    • Journal of Sericultural and Entomological Science
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    • v.43 no.2
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    • pp.116-124
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    • 2001
  • To improve the functional properties as a food, silk fibroin was phosphorylated with STMP In the phosphorylation reaction of silk fibroin, the degree of phosphorylation was increased with high alkali index and treatment temperature. Depending on treatment time and concentration of STMP it was rapidly increased up to 1hr. and 50%, but slowly above that time and 100%. It was indicated in the results of FT-IR analysis and $\^$31/p NMR spectroscopy of phosphorylated fibroin that it had a close ∝-helix and poly-phosphate structure. The more phosphorylation of fibroin made more turbidity, foam expansion and foam stability, but less solubility. Emulsifying activity was increased up to P100, but slightly decreased above Pl00 and emulsifying stability was constantly increased on the progressing of phosphorylation.

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Chemical Synthesis of Oligodeoxyribonucleotide ; Improvement of Deoxyribonucleoside Phosphorylation and Dideoxyribonucleotide Synthesis (Oligodeoxyribonucleotide의 화학적 합성 ; Deoxyribonucleoside의 인산화와 이량체 합성 방법의 개선)

  • Sang Jik Lee;Byung Soo Song;Jong Dae Kim
    • Journal of the Korean Chemical Society
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    • v.31 no.1
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    • pp.84-93
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    • 1987
  • The study was done with a focus on making the optimum condition on phosphorylation of deoxyribonucleoside with o-chlorophenylphosphoroditriazole as a phosphorylating agent. The result showed that the addition of 5 volume % pyridine to the dioxane solution accelerated the rate of reaction to a great extent and turned out to nearly quantitative yields on phosphorylation. On the basis of this improvement of optimum reaction conditions, a more efficient method to synthesize all-protected dideoxyribonucleotide from N, 5-O-blocked deoxyribonucleoside was developed. The dodecamer with a Hind Ⅲ recognition site was readily synthesized from five different dimers which were prepared through the newly improved method.

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Dehydration of Alcohol Solutions Through Crosslinked Chitosan Composite Membranes II. Dehydration of Ethanol Solution Through Modified Chitosan Composite Membranes (가교키토산 복합막을 통한 알콜수용액의 탈수 II. 변성 키토산 복합막을 통한 에탄올의 탈수)

  • 이영무;남상용;유제강;류경옥
    • Membrane Journal
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    • v.6 no.4
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    • pp.242-249
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    • 1996
  • To improve pervaporation performance of water/ethanol mixtures, chitosan/poly(vinyl alcohol) blended and phosphorylated chitosan composite membranes were prepared. Chitosan/poly(vinyl alcohol) blends were prepared with various blend ratios and then crosslinked with glutaraldehyde by two methods. With increasing crosslinking agent content and crosslinking times separation factor increased and permeate flux decreased. Separation factor of the membrane which contains glutaraldehyde as a crosslinking agent was higher than that of the membrane surface crosslinked. Phosphorylated chitosan was prepared with various reaction times and composite membrane was prepared. As reaction times increased, the separation factor increased with high affinity for water.

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A study on the preparation of phosphatic calcium compounds using the shell resources (패각을 이용한 인산칼슘계 화합물의 제조에 관한 연구)

  • 이인곤;김판채
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.10 no.2
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    • pp.171-176
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    • 2000
  • The phosphatic calcium compounds such as calcium hydrogen phosphate, bone ash, hydroxyapatite and tricalcium phosphate were prepared using the high purity calcium hydroxide and calcium carbonate obtained from shell resources. Calcium hydrogen phosphate had been prepared using the high purity calcium hydroxide and phosphoric acid solution. Using the calcium hydrogen phosphate as a starting materials, bone ash have been prepared by solid state reaction method and hydroxyapatite could be obtained by hydrothermal treatment method, respectively. The tricalcium phosphate was prepared by the solid state reaction of a stoichiometic mixture of bone ash and high purity calcium carbonate. In this paper, the optimal preparation process and conditions of phosphatic calcium compounds were established.

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Characteristics and Catalytic Activities of 12-Molybdophosphoricacid Modified by Ring Opening Polymerization of Tetrahydrofuran (테트라하이드로퓨란의 개환 중합반응에 의해 변형된 12-몰리브도인산의 특성 및 촉매 반응성)

  • Park, Gyo Ik;Lee, Wha Young;Song, In Kyu
    • Applied Chemistry for Engineering
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    • v.9 no.7
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    • pp.1018-1022
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    • 1998
  • Characteristics and catalytic activities of 12-molybdophosphoricacid catalysts modified by ring opening polymerization of tetrahydrofuran (THF) were studied in this work. 12-Molybdophosphoricacid catalysts modified by THF showed higher conversions and product yields than the mother acid in the vapor-phase ethanol conversion. It was believed that the enhanced catalytic activities of modified 12-molybdophosphoricacids were due to structural flexibility of heteropolyanions and weakened hydrogen bond around heteropolyanions. THF interacted with protons and crystalline water molecules of heteropolyacids. The interaction between THF and heteropolyanoins, which crystalline water molecules act as intermediary, was the dominant factor deciding the catalytic activities of modified 12-molybophosphoricacids.

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Immobilization Characteristics of Copper Contaminated Soil Using Phosphate( I ) (인산염을 이용한 Cu 오염토양의 고정화 특성 연구 ( I ))

  • Lee, Eui-Sang;Sung, Ho-Young;Kim, Ji-Young;Lee, Ju-Goang
    • Proceedings of the KAIS Fall Conference
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    • pp.291-293
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    • 2007
  • 인산염을 이용한 구리 고정화 실험의 토양적용가능성을 평가하고자 액상반응실험과 고정화물의 재용출 실험을 수행하였다. 액상반응실험 결과 인산염과 알칼리제 1mole을 넣은 반응에서 99.9% 의 구리제거효율을 보였고 구리의 재용출 농도는 0.64mg/L로 초기농도인 1000mg/L보다 현저히 낮은 값을 나타내었다.

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Phosphorylated Proteins of Mitogen Stimulated-Rat Peripheral Blood Lymphocytes (분열유발인자에 의한 흰쥐 림프구 단백의 인산화)

  • Jou, I-Lo;Ko, Sung-Soo;Ahn, Young-Soo
    • The Korean Journal of Pharmacology
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    • v.29 no.1
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    • pp.121-130
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    • 1993
  • This study was done to classify the proteins involved in the specific phosphorylation using the rat peripheral blood lymphocytes (rPBL) stimulated with mitogens, phorbol 12-myristate 13-acetate (PMA) and concanavalin A (Con A). The lymphocytes were incubated with $^{32}P-orthophosphate$ before PMA or Con A stimulation. The migration patterns of the phosphorylated proteins of mitogen-treated rPBL in two dimensional electrophoretic fields were analyzed after autoradiography. The stimulation of the lymphocytes with PMA and Con A increased the phosphorylation of thirteen protein fractions. The phosphorylation intensities of the protein spots differ to the treatments of the cells with specific kinase inhibitors, H-7 and W-7. These protein fractions were grouped into 3 classes, namely, PKC-mediated, CaM kinase-mediated, and other kinase mediated proteins. The effect of the duration of the stimulation on the phosphorylated behaviors occurred concurrently, not sequentially, although each individual protein fraction had a different time for the peak phosphorylation during the stimulation period upto 30 minutes. The phosphoproteins found in the cytosolic soluble fraction were phosphorylated prior to those in the pellet, whose phosphorylations were sustained at a high level for over 10 minutes. The above results suggest that the early events in lymphocyte activation involve 3 different sets of proteins which are phosphorylated by CaM kinase, PKC and other kinases, and those kinases do not work sequentially, but rather, independently or cooperatively.

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