• Title, Summary, Keyword: 인산화반응

Search Result 224, Processing Time 0.053 seconds

Involvement of Serine Phosphorylation of Spinal Cord NR-2B Subunit of the N-methyl-D-aspartate Receptor Following Electroacupuncture Stimulation (전침자극이 척수 N-methy1-D-aspartate receptor외 NR-2B Subunit 인산화에 미치는 영향)

  • Kang, Byeol-Rim;Choi, Byung-Tae;Yoon, Hyun-Min;Min, Young-Kwang;Ahn, Chang-Beohn
    • Journal of Acupuncture Research
    • /
    • v.24 no.2
    • /
    • pp.63-71
    • /
    • 2007
  • 목적 : 저주파에 해당하는 2Hz 전침 자극이 척수 N-methyl-D-aspartate receptor (NMDAR)의 NR-2B subunit의 발현 및 인산화에 미치는 영향을 조사하였다. 방법 : Sprague-Dawley계 흰쥐를 Storkson등의 방법에 의해 척수막의 지주막하강에 catheter를 삽입하는 수술을 행한 후 마비등의 척수 손상을 나타내지 않는 개체를 대상으로 하였다. N-methyl-D-aspartate (NMDA) antagonist인 D-2-amino-5- phosphonopentanoic acid (AP-5)를 투여한 후 족삼리와 삼음교에 해당하는 부위에 30분간 전침 자극하였다. 무통각 여부는 hot plate test를 시행하였으며 NMDAR NR-2 subunit 발현과 인산화 여부는 Western blot과 면역조직화학적으로 살펴보았다. 결과 : 전침 무통각은 전침 자극 후 180분 후까지 지속되었으며 NMDA antagonist인 AP-5를 투여하였을 때 전침 무통각이 저하되었으나 유의성은 나타내지 않았다. Western blot 분석으로 보아 NMDAR NR-2B 및 인산화 NR-2B의 발현은 전침자극에 의해 미약한 증가를 보이나 AP-5투여에 의해 현저한 저해를 보였다. 면역조직화학에 의한 척수배각 구역별 발현을 보면 NMDAR NR-2B 및 인산화 NR-2B는 전 배각에 걸쳐 관찰되나 경부(층판 V-VI)에서 약한 반응을 보였다. 전침 자극에 의한 각 군별 NR-2B 발현은 유의한 차이를 보여 주지 않았으나 인산화 NR-2B는 천층(층판I-II) 및 고유핵 층판(III-IV)에서 유의성 있는 증가를 보였다. 전침 자극시 AP-5 투여는 유의성은 보이지 않았으나 인산화 NR-2B 발현을 저해하였다. 결론: 저주파 2Hz 전침에 의한 무통각은 NMDA antagonist인 AP-5 투여에 의해 저해될 뿐 아니라 NMDAR NR-2B subunit의 인산화를 저해하는 것으로 보아 전침 무통각의 과정에 NMDAR 및 NMDAR NR-2B의 인산화가 관여함을 알 수 있다.

  • PDF

Ozone-water Treatment on the Morphological Changes of Endosperm cell and the activity of Acid Phosphatase during Soybean(Glycine max) Germination (대두 발아중 오존수 처리가 acid phoshatase 및 배유세포의 형태학적인 변화)

  • 박홍덕
    • Journal of Life Science
    • /
    • v.11 no.5
    • /
    • pp.489-495
    • /
    • 2001
  • The effect of ozone-water treatment on the morphological change of endosperm cells and the activity of acid phosphatase during Glycine max germination was investigated with electron microscope. Acid phosphatase showed the activity in the cell organelles of germinating endosperm of seed. it's activity occurrs in 12 hrs cultivation after 0.5 ppm ozone-water treatment. As the differentiation of endosperm, reaction products of the acid phosphatase appear to be accumulated invacuole after treatment of ozone-water. This result confirm that acid phosphatase is inveolved in the decomposition and translation of the intracellular storage materials. The characteristics of grganelle in the endosperm cell during germination were discussed.

  • PDF

Long-term Leaching Characteristics of Lead Contaminated Soils treated with Soluble Phosphate (액상인산염으로 처리된 납 오염토양 복원의 장기용출 특성)

  • Lee Eui-Sang
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.7 no.3
    • /
    • pp.453-457
    • /
    • 2006
  • A long-term leaching column experiment was performed to evaluate the teachability of the stabilized lead-contaminated soil using soluble phosphate. The study shows that Pb in the leachate was little detected and the remaining $PO_4-P$ concentration kept below 0.1 mg/L due to the formation of geochemically stable lead phosphate minerals from the reactions of labile soil Pb forms with the added soluble phosphate salt. After the experiment, there was no Pb migration from the top to the bottom of the stabilized soil column. But the Pb concentrations of the 12 soil samples from the control column decreased with the increase of the soil depth.

  • PDF

Ammoxidation of Methylpyrazine over Molybdenum Phosphate Catalyst (몰리브덴인산화물 촉매에 의한 메틸피라진의 가암모니아 산화반응)

  • Shin, Chae-Ho;Chang, Tae-Sun;Cho, Deug-Hee;Lee, Dong-Koo;Lee, Young K.
    • Applied Chemistry for Engineering
    • /
    • v.8 no.5
    • /
    • pp.749-755
    • /
    • 1997
  • Molybdenum phosphate(P/Mo = 0.6) has been synthesized with ammonium molybdate and phosphoric acid under aqueous solution. The kinetics of the ammoxidation of methylpyrazine over molybdenum phosphate catalyst was investigated with the variation of reaction temperature and partial pressure of methylpyrazine, oxygen and ammonia, respectively at atmospheric pressure. The catalytic activity was constant for 300hrs operation under our experimental conditions. Under the steady-state condition, the rate equation of methylpyrazine was shown as $-r=kP_{MP}P_{NH3}{^0}P_{O2}{^{\gamma}}({\gamma}=2.2;1.3{\leq}P_{O2}(kPa){\leq}4)$. The apparent activation energy was 29.6kcal/mol below 623K. The main product obtained in the ammoxidation of methylpyrazine is cyanopyrazine whose selectively was kept always over 90% regardless of conversion.

  • PDF

Effects of Oxidant, Reductant Treatment and Its Phosphorylation on Qualities and Functional Properties of Defatted Rice Bran Protein Isolates (산화.환원제처리 및 인산화가 분리탈지미강단백의 품질 및 기능적 성질에 미치는 영향)

  • Park, Wan-Kyu;Kim, Seung-Yeol;Lee, Ka-Sun
    • Korean Journal of Food Science and Technology
    • /
    • v.20 no.1
    • /
    • pp.106-111
    • /
    • 1988
  • Comparative effects of oxidant, reductant treatment and its phosphorylation on qualities and functional properties of defatted rice bran protein isolates were investigated. Effects of oxidant and reductant treatment were that essential amino acid content of protein isolates was high and its color, pepsin digestibility were good. The phosphorylated defatted rice bran protein isolated was taken by incubating sodium trimeta phosphate in aqueous solution at pH 10.5 and $35^{\circ}C$ for 3 hours and its protein score was 55. Functional properties such as solubility, whipping activity and foam stability were much improved. But color, pepsin digestibility, bulk density and fat absorption were not affected by phosphorylation.

  • PDF

Investigations of Reactive Intermediate formed in the oxidation of 4-Methyl-2,6,7-trioxa-1-phosphabicyclo[2,2,2] octane 1-sulfide (4Methyl-2,6,7-trioxa-1-phosphabicyclo[2,2,2]octanel-sulfide의 산화반응 중 생성되는 반응성 중간체에 대한 연구)

  • Kim, Jeong-Han
    • Applied Biological Chemistry
    • /
    • v.39 no.3
    • /
    • pp.227-234
    • /
    • 1996
  • 4-Methyl-2,6,7-trioxa-1-phosphabicyclo [2,2,2] octane 1-sulfide (BPS), 4-Methyl-2,6,7-trioxa-1-phosphabicyclo[2,2,2] octane 1-oxide (BPO) and related monocyclic methylphosphates were prepared and the oxidation of BPS with MCPBA in chlorofrom or methanol was carried out to investigate the formation of reactive intermediates and reaction mechanism. BPO was the only product in chloroform while in methanol isomeric monocyclic methylphosphates were formed through opening of bicylic structure with subsequent phosphorylation of methanol by reactive intermediate formed in reaction. Formation of little amount of BPO was also observed. The structure of phosphorylating intermediate was probed with various spectroscopic methods and monocyclic methyl sulfenyl ester was suggested as a possible structure.

  • PDF

Heterogeneously Catalyzed Oxidations of Cyclopentene and of 1-Pentene (시클로펜텐과 1-펜텐의 불균일 촉매 산화반응)

  • Yang, Hyun S.;Kim, Young H.
    • Applied Chemistry for Engineering
    • /
    • v.7 no.5
    • /
    • pp.888-901
    • /
    • 1996
  • Oxidations of cyclopentene and of 1-pentene with air have been studied on a V/Mo/P/Al/Ti-mixed oxide catalyst in a fixed bed integral reactor. At high levels of conversion maleic anhydride was in each case produced as the major organic product, along with minor amounts of phthalic anhydride and, only starting from 1-pentene, also of citraconic anhydride. At lower levels of conversion a total of 30 organic products have been identified, some of which may be intermediates on the way from the substrates to the three anhydrides mentioned above. Based on the dependence of selectivities of the organic products on conversion, reaction schemes for the formation of maleic anhydride, phthalic anhydride and citraconic anhydride have been proposed. Oxidation at $310^{\circ}C$ led to increasing conversions and selectivities for maleic anhydride with decreasing space velocities. The highest selectivities for maleic anhydride were obtained at conversion of ca. 100%. Oxidation at a constant space velocity of $2{\cdot}10^4h^{-1}$ led to increasing conversions with increasing temperatures in the range of $300^{\circ}C{\sim}420^{\circ}C$, while the selectivity for maleic anhydride passed through a maximum value of ca. 39% at $370^{\circ}C$ in the oxidation of cyclopentene and a maximum value of ca. 30% at $400^{\circ}C$ in the oxidation of 1-pentene.

  • PDF

Effect of $GA_3$ on Ribosomal Protein Phosphorylation in Germinating Zea mays (발아 중인 옥수수에서 리보조옴 단백질의 인산화반응에 미치는 $GA_3$의 효과)

  • 안경섭
    • Journal of Plant Biology
    • /
    • v.33 no.1
    • /
    • pp.59-64
    • /
    • 1990
  • In order to study the effect of GA3 on the phosphorylation of ribosomal proteins during germination in Zea mays, ribosomal proteins were labelled with 32P, extracted, electrophoresed and autoradiographed. There are five phosphorylated ribosomal proteins. One of these is in 40S subunit and has molecular weight of 33,000 daltons. Others are in 60S subunit and have molecular weights of 37,000, 16,000, 15,200 and 13,500, respectively. Phosphorylation of ribosomal proteins was increased maximum 47.7% in shoots of Zea mays treated with GA3.

  • PDF

Microstructure Evolution of Solid State Reacted HAp/β-TCP Composite Powders by Post-Treatment Processing (후처리공정에 따른 고상반응 β-TCP/HAp 복합분체의 미세구조 변화)

  • 박영민;양태영;박상희;윤석영;박홍채
    • Journal of the Korean Ceramic Society
    • /
    • v.41 no.8
    • /
    • pp.582-587
    • /
    • 2004
  • Biphasic Calcium Phosphate (BCP) consisted of hydroxyapatite (HAp) and $\beta$-tricalcium phosphate (P-TCP) has been prepared by solid state reaction. The size reduction of the resultant BCP agglomerate was carried out by reaction with hot water under atmospheric condition uld also under high pressure using an autoclave. The influence of processing conditions on the change of crystalline phase and composition, relative amount of constituent, specific surface area, and microstructure was investigated by means of XRD, FT-IR, BET method using a nitrogen adsorption and SEM.

Assessment of the Struvite Crystallization Process for Phosphate Removal and Recovery from a Sludge Treatment System of a Domestic Wastewater Treatment Plant (하수처리장 슬러지처리 계통에서의 인 제거 및 회수를 위한 Struvite 결정화 공정 적용성 평가)

  • Baek, Seung Ryong;Lee, Byung Joon
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.39 no.8
    • /
    • pp.462-469
    • /
    • 2017
  • Eutrophication and shortage of phosphate ore raise the necessity of phosphate removal and recovery from wastewater treatment plants. Especially, a sludge treatment system containing highly concentrated phosphate should be targeted for phosphate removal and recovery. This study thus aimed to evaluate the capability of the struvite crystallization process for phosphate removal and recovery from a sludge treatment system of a wastewater treatment plant. Analysis on phosphate concentrations and masses in the sludge treatment system revealed that digested sludge and centrate have phosphate concentrations and masses, high enough to adopt the struvite crystallization process. Chemical equilibrium modeling indicated that the struvite crystallization reaction substantially occurred with pH higher than 8 and $Mg^{2+}$ concentration 1.2 times higher than its theoretical requirement. A series of batch tests with digested sludge and centrate indicated that the phosphate removal reaction by struvite crystallization followed a first-order kinetics and reached over 80% removal efficiency at equilibrium. Aeration in the batch tests was found to purge $CO_2$ in sludge or centrate and increase pH up to 8.7, without adding NaOH. Thus, we concluded that the struvite crystallization process could be an efficient and economical process for phosphate removal and recovery from a wastewater treatment plant.