• Title, Summary, Keyword: 인지질

Search Result 1,061, Processing Time 0.04 seconds

The Thermotropic Phase Behaviors of Artificial Phospholipid Liposomes Incorporated with Soyasaponin (대두사포닌이 침투된 인공 인지질 생체유사막의 열에 의한 상변화에 관한 연구)

  • Kim, Nam-Hong;Roh, Sung-Bae
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.22 no.3
    • /
    • pp.323-327
    • /
    • 1993
  • The effect of soyasaponin on the liposomal phospholipid membrane was investigated by differential scanning calorimetry (DSC). Soyasaponins were obtained and the enthalpy changes and the sizes of cooperative unit of the transition were calculated. The thermograms of L-$\alpha$-dimyristoyl phosphatidylcholine (DMPC) incorporated with soyasaponin showed that the phase transition temperature was significantly lowered and the peak was broadened. This was attributed to the possibility that incorporation of soyasaponin into the lipid bilayers reduced the cooperative unit of phospholipid bilayers. These results indicate soyasaponin might have significant effect on the fluidity of biological membrane.

  • PDF

Antioxidant Effect of Soyasaponin on the Liposomal Phospholipid Membrane (인지질 Loposome 에 미치는 대두 Saponin의 항산화효과)

  • 신미옥;배송자;김남홍
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.21 no.4
    • /
    • pp.381-385
    • /
    • 1992
  • The effect of antioxidant activity of soyasaponin on the liposomal phospholipid membrane were investigated by spectrophotometry. The oxidation index and oxidation rate of ${\alpha}-tocopherol$ containing egg phosphatidylcholine (EPC) liposomes were markedly decreased in the presence of soyasaponin relative to those of purephospholipid liposmes. ${\alpha}-tocopherol$ containing liposomes delayed the oxidation of liposomes. Especially soyasaponin stimulated the antioxidant activity of ${\alpha}-tocopheroal$ in liposomes. These results indicates that soyasaponin in liposomes had some additive effect on antioxidant of ${\alpha}-tocopherol$ toward liposomes.

  • PDF

A 2H solid-state NMR study on the lipid phase change in the presence of an antimicrobial peptide (항균성 펩타이드와 혼합된 인지질 분자의 상 변화에 대한 고체 중수소 핵자기 공명 연구)

  • Choi, Hyung Geun;Kim, Chul
    • Analytical Science and Technology
    • /
    • v.26 no.1
    • /
    • pp.61-66
    • /
    • 2013
  • The activity of an antimicrobial peptide, protegrin-1, on the well-aligned lipid bilayer deposited on a thin coverglass plate was investigated by $^2H$ solid-state NMR spectroscopy. Orientational distribution and molecular motion in the lipid bilayer were determined from $^2H$ solid-state NMR spectrum. Reorientational motion of lipid molecules in the vacuum-dried state was found to be small but their orientational distribution was not able to be determined. As storage times were longer, the order of the alignment of lipid molecules in the lipid bilayer and percentages involved in the toroidal pore structures increased. We found that much longer time is required to get the equilibrium state of the peptide-lipid mixture under our experimental condition for investigating the action of the antimicrobial peptide like protegrin-1 on the lipid bilayers deposited on the thin coverglass plates.

Phospholipid and Fatty Acid Metabolism in Escherichia coli-On the Effects of Various Metal Compounds

  • Ma, Hae-Young;Choi, Jong-Hak;Lee, Chong-Sam
    • Journal of Environmental Health Sciences
    • /
    • v.22 no.2
    • /
    • pp.114-123
    • /
    • 1996
  • Escherichia coli에 copper chloride 50 ppm, manganese chloride 100 ppm, nickel chloride 100 ppm을 각각 처리하여 세포를 배양하는 동안에 이들 세포에서 일어나는 인지질 생합성 및 지방산 조성의 변화를 대조구와 비교하여 분석하였다. 세포의 생장과 total lipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, cardiolipin은 대조구조에 비해 금속 화합물 처리구에서 저해되었는데 nickel chloride가 가장 큰 억제 효과를 나타내었다. 그러나 phosphatidylinositol은 금속화합물의 영향을 받지 않았다. 인지질 생합성에 이용된 주요 지방산은 대조구는 palmitic acid(평균 25.47%)와 palmitoleic acid(평균 12.27%)가 인지질 생합성에 도입되었고 copper chloride 처리구는 palmitic acid(평균 30.13%)와 stearic acid(평균 9.12%)로 나타났다. manganese chloride 처리구와 nickel chloride 처리구에서는 모두 palmitic acid(평균 24.16%, 평균 21.77%)와 linoleic acid(평균 9.48%, 평균 11.88%)가 인지질 생합성에 이용된 주요 지방산으로 분석되었다.

  • PDF

Lipid and Fatty Acid Composition of Culled Laying Hen (산란노계육의 지질함량 및 지방산 조성)

  • 문윤희;공양숙
    • Korean Journal of Poultry Science
    • /
    • v.16 no.3
    • /
    • pp.169-174
    • /
    • 1989
  • Culled laying hens used in this study were Arbor acres, which reared to S 35 days old on commerical formula feed for chicken. Liver, gizzard, breast and thigh muscles separated from each carcass, and total lipid was extracted and fractionated to neutral, phospho and glycolipid and then fatty acid composition were analyzed. Liver had the highest level of. total lipid, and breast tissue had the least among tissues tested. The neutral, phospho and glycolipid contents of total lipid had more thigh, breast and gizzard than other tissues, respectively. The major fatty acid in total and neutral lipid were palmitic, stearic, oleic and linoleic acid. And the major fatty acid in phospholipid was palmitic, stearic, oleic, linoleic, arachidonic and docosahexaenoic acid, and palmitic, palmitoleic, stearic, oleic and linoleic acid in case of glycolipid. The fatty acid contents of neutral, phospho and glycolipid in total lipid had more oleic, docosahexaenoic and linoleic acid than other lipid, respectively. Contents of unsaturated fatty acid of total and neutral lipid were comparatively high in thigh, and phospho and glycolipid were high in breast and liver, respectively. Contents of Polyunsaturated fatty acids were comparatively high in phospholipids than other lipids.

  • PDF

Effect of Antibiotics on Phospholipid Biosynthesis and Fatty Acid Composition in Chlorella ellipsoidea Chloroplasts (Chlorella ellipsoidea 엽록체의 인지질 생합성 및 지방산 조성에 미치는 항생제의 효과)

  • 조수열
    • Journal of Plant Biology
    • /
    • v.35 no.1
    • /
    • pp.25-36
    • /
    • 1992
  • The effects of amphotericin B ($150\;\mu\textrm{g}/ml$) and cycloheximide ($10\;\mu\textrm{g}/ml$) on the biosynthesis of phospholipid and the composition of fatty acids in chloroplasts isolated from ChZorella were analyzed. The contents of the total lipid and phospholipid (PC, PE, PI) in treatment with antibiotics were lower compared with the control. In the whole cell system, the major fatty acids utilized for biosynthesis of phospholipid were palmitic acid (31.96%) and linoleic acid (16.96%) in control while those were palmitic acid (36.15%) and linolenic acid (16.71%) in treatment with amphotericin B. And in treatment with cycloheximide, palmitic acid (31.90%) and stearic acid (15.32%) were used in phospholipid formation. The major fatty acids in chloroplasts were analyzed as to be palmitic acid and linolenic acid in control (33.75%, 18.90%) and in treatment with amphotericin B (36.75%, 9.46%). However, it was shown that the major fatty acids in chloroplasts treated with cycloheximide were palmitic acid (28.01%) and oleic acid (19.27%).9.27%).

  • PDF

Phospholipids Isolation from Squid Viscera Residues After Supercritical Carbon Dioxide Extraction (오징어 내장의 초임계 이산화탄소 추출 잔류물로부터 인지질의 분리)

  • U, Pyoung-Ook;Chun, Byung-Soo
    • Korean Chemical Engineering Research
    • /
    • v.48 no.6
    • /
    • pp.741-746
    • /
    • 2010
  • Phospholipids were recovered from squid viscera residues by ethanol extraction after supercritical carbon dioxide($SCO_2$) extraction and from squid viscera was not processed $SCO_2$ by various organic solvent extraction. $SCO_2$ extraction were performed at $45^{\circ}C$ and 20 MPa for removal of non polar lipid molecules from freeze dried squid viscera sample. Phospholipids were extracted from freeze dried squid viscera sample by chloroform, hexane, methanol, and ethanol and from $SCO_2$extracted squid viscera sample by ethanol. The pH was fixed at 5.7 for all phospholipids extraction conditions. Phospholipid classes were analyzed by HPLC equipped with evaporative light scattering detector (ELSD). Phosphatidyl choline(PC) extracted by ethanol from $SCO_2$ extracted residues was higher than that of extracted by ethanol from squid viscera. But phosphatidyl ethanolamine(PE) and phosphatidic acid(PA) were extracted higher percentage in raw squid viscera. The fatty acid compositions in phospholipids extract by ethanol extract from $SCO_2$ extracted residues were analyzed by gas chromatography(GC). Docosahexanoic acid(DHA) was found in highest percentage in phospholipid extract.

A Study on the Electrochemical Properties of Langmuir-Blodgett Nano-film Mixed with Polyimide and Phospholipid (폴리이미드와 인지질 혼합물의 나노 Langmuir-Blodgett막의 전기화학적 특성에 관한 연구)

  • Park, Keun-Ho
    • Journal of the Korean Applied Science and Technology
    • /
    • v.29 no.3
    • /
    • pp.421-428
    • /
    • 2012
  • We investigated an electrochemical properties for Langmuir-Blodgett (LB) nano-films of polyimide and phospholipid mixture. LB films of polyamic acid and phospholipid monolayer were deposited by the Langmuir-Blodgett method on the indium tin oxide(ITO) glass. The electrochemical properties measured by cyclic voltammetry with three-electrode system(an Ag/AgCl reference electrode, a platinum wire counter electrode and LB film-coated ITO working electrode) in $KClO_4$ solution. The current of reduction and oxidation range was measured from 1650 mV to -1350 mV, continuously. The scan rates were 50, 100, 150, 200 and 250 mV/s, respectively. As a result, monolayer LB films of polyamic acid and phospholipid mixture was appeared on irreversible process caused by the reduction current from the cyclic voltammogram. Diffusion coefficient (D) effect in the polyamic acid and phospholipid mixture was used in the LAPC with LLPC fewer than the diffusion coefficient values.

Studies on the Lipids of 'Bugbangjohgae' Spisula sachalinensis (북방 조개의 유지에 관한 연구)

  • JOH Yong Goe;HATA Mitsuo
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.9 no.3
    • /
    • pp.195-202
    • /
    • 1976
  • The present investigation was performed to find the lipid composition of the total lipids, the fatty acid components of the neutral lipids and the phospholipids, and the composition of sterols, from Spisula sachalinensis. The results obtained are as follows ; 1) The main components of the total lipids are phospholipids$(43.1\%)$, triglyceride$(36.2\%) $, and sterol $(10.3\%)$. 2) The phospholipids are mainly composed of phosphatidyl choline, phosphatidyl ethanols, mine, phosphatidal ethanolamine and phosphatidyl serine. 3) The main fatty acids of the neutral lipids, the ethanolamine phospholipids and choline phospholpids, are C20:5, C16:1, C16:0, C20:5, C18:0, C22:6 and C16:0, C20:5, C22:6, respectively. Oleic acid content of all fractions is very small compared with one of gastropoda lipids and fish oil. 4) Most of plasmalogen are present in the ethanolamine phospholipids and only trace of plasmalogen in the choline phospholipids. 5) Sterols to be found are 22-trans-norcholesta-5, 22-diene-$3\beta$-ol, 22-dehydrocholesterol, cholesterol, brassicasterol, 24-methylenecho-lesterol and $\beta-sitosterol$.

  • PDF

Effects of Phytohromones on Biosynthesis of Phospholipids and Their Fatty Acid Compositions in Chlorella ellipsoidea Chloroplast (Chlorella ellipsoidea 엽록체의 인지질 생합성 및 지방산 조성에 미치는 식물호르몬의 효과)

  • 나형심
    • Journal of Plant Biology
    • /
    • v.35 no.4
    • /
    • pp.371-383
    • /
    • 1992
  • The effects of IAA $(10^{-3}M)\;an;GA_3\;(2{\times}10^{-8}\;M)$ on the biosynthesis of phospholipids and their fatty acid compositions in chloroplast isolated from Chlorella ellipsidea were analyzed. Growth ratio and the levels of total lipids and phospholipids in whole cell system and chloroplast treated with phytohormones were higher than those of control, and when treated with GAl. phosphatidylcholine content was predominantly higher than that of control. It was showed that in whole cell system the fatty acid utilized for biosynthesis of phopholipids was 27.43% for palmitic acid in control, while that was 20.25% for linolenic acid in treatment with 1M. In treatment with $GA_3$, 23.17% for linolenic acid was used in phospholipid formation. The major fatty acid in the chloroplst system was analyzed to be 35.67% for palmitic acid in control and in treatment with 1M, 24.91% for linolenic acid was used in phospholipid formation, while in treatment with $GA_3$, major fatty acid of phospholipids was 22.80% for linoleic acid.c acid.

  • PDF