• Title, Summary, Keyword: 3-methylhistidine

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Determination and Application of 3-Methylhistidine Levels in Urine (뇨(尿)중 3-Methylhistidine 함량의 측정과 이용)

  • 정수현;서형주
    • The Korean Journal of Food And Nutrition
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    • v.9 no.2
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    • pp.213-216
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    • 1996
  • A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 M sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhlstidine from urine control were 95% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary .3-methylhistidine content of volunteers increased significantly after weight training.

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Effect of Exercise on Serum Lipids, Urinary Creatinine and 3-Methylhistidine in Human (운동(運動)이 혈청(血淸) 지질(脂質) 및 뇨중(尿中) Creatinine과 Methylhistidine 농도(濃度)에 미치는 영향(影響))

  • Song, Kyung-Hee;Choi, Hay-Mie
    • Journal of Nutrition and Health
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    • v.17 no.4
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    • pp.305-312
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    • 1984
  • The effect of exercise on serum cholesterol and triglyceride concentration and on urinary creatinine and 3-methylhistidine excretion was studied in eight healthy women, aged 21 to 25yr. Subjects were tested before and after a 3-day experimental period, during which 1000 times rope-jumping a day were performed. Serum cholesterol and triglyceride levels decreased significantly after exercise. Urinary excretion of creatinine and 3-methylhistidine slightly increased after exorcise.

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Effect of Maternal Dietary Restriction and Rehabilitation on the Muscle Protein Breakdown of Young Rats (어미 쥐의 식이제한과 식이회복이 새끼쥐의 근육단백질 분해에 미치는 영향)

  • Lim, Kyeong-Sook;Choi, Hay-Mie;Byun, Ki-Won
    • Journal of Nutrition and Health
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    • v.15 no.1
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    • pp.62-69
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    • 1982
  • A quantitative restriction of maternal diet was given to the Sprague Dawley rats during the lactation. The control group were fed a commercial diet ad libitum throughout the experimental period. Dietary restriction started from birth to weaning in deficient group. After weaning at 21 days, all offsprings were fed the same diet ad libitum for 4 weeks of rehabilitation. They were analyzed for body weight, muscle protein, muscle 3-methylhistidine and serum protein level of offsprings at 0, 1, 2, 3, 5, 7 weeks. Body weight of offsprings of deficient group were significantly lower than the control group, but after rehabilitation there were no significant differences between two groups. Maternal dietary restriction caused a decrease in the 3-methylhistidine, protein content in muscle and total protein level in serum, and rehabilitation after weaning induced a marked increase. These results suggest that there is a quantitative reduction in 3-methylhistidine metabolism in the undernourished rats and these reductions can be nearly recovered by 4 weeks of dietary rehabilitation after weaning.

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Effect of Plant and Animal Proteins on Serum Lipids and Urinary Methylhistidine in Human (동물성(動物性)과 식물성(植物性) 단백질(蛋白質) 섭취(攝取)가 혈청(血淸) 지질(脂質) 및 뇨중(尿中) Methylhistidine에 미치는 영향(影響))

  • Song, Kyung-Hee;Choi, Hay-Mie
    • Journal of Nutrition and Health
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    • v.15 no.3
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    • pp.212-222
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    • 1982
  • The effect of changing dietary protein sources (meat vs. textured soy protein, TSP) on serum cholesterol and triglyceride concentration and on urinary creatinine and 3-methylhistidine(3-MeHis) excretion was studied in eight healthy women. All subjects were put on the control diet, meat diet, and TSP diet for 3 days in each diet regimen and had 4-day adaptation period between the regimens. The 24-hour urine was collected on the third day of each diet period and fasting blood was sampled on the morning of the fourth day. Serum cholesterol and triglyceride concentrations increased significantly after the meat diet when compared with the control diet, but decreased significantly after the TSP diet. Urinary excretion of creatinine and 3-methylhistidine decreased significantly after the TSP diet when compared with the meat diet.

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HPLC Analysis of Methylated Amino Acids : Methylated Amino Acids on HPLC

  • Park, Kwang-Sook;Hong, Sung-Youl;Lee, Hyang-Woo;Kim, Snag-Duk;Paik, Woon-Ki
    • Archives of Pharmacal Research
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    • v.9 no.1
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    • pp.15-18
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    • 1986
  • Various naturally occuring methylated amino acid derivatives were resolved on high performance liquid chromatography (HPLC), using o-phthadialdehyde as a fluorogenic reagent. We separated .$\varepsilon$-N-monomethyllysine, $\varepsilon$-N- dimethyllysine, and $\varepsilon$-N-acetyllysine from lysine derivatives. $N^{G}$-Monomethylarginine and $N^{G}$-dimethylarginine were separated from arginine derivatives. However, $\varepsilon$-N-monomethyllsine and $\varepsilon$-N-trimethyllysine, $N^{G}$, $N^{G}$-dimethylarginine and $N^{G}$, $N^{G}$-dimethylarginine were not resolved under the conditions employed. S-Methylmethionine, S-methylcysteine, and 1-N-methylhistidine or 3-N-methylhistidine were clearly separated from their reference amino acids, even though 1-N-methyl-and 3-N-methylhistidine coul not be separated.

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Seasonal Change of Plasma Free Amino Acids with Special Reference to 3-Methylhistidine in Racehorses

  • Sawada, Kumiko;Li, Jun You;Kuribayashi, Yasuko;Itabashi, Hisao
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.12
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    • pp.1779-1784
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    • 2008
  • Seasonal changes in the concentration of free amino acids were determined monthly in plasma from the jugular blood of 50 thoroughbred racehorses that compete during the night between June and September and during the day between October and January. The concentration of most free amino acids remained relatively constant between June and January. However, those of glutamic acid, alanine, isoleucine and lysine tended to decrease and that of arginine (Arg) significantly decreased between July and September. The concentration of methionine (Met) gradually increased between June and September and significantly decreased thereafter. The concentration of 3-methylhistidine (3-MH) significantly increased between July and September and decreased thereafter. There were significant correlation between 3-MH and Arg. In conclusion, this study provided evidence of significant seasonal change in plasma 3-MH, Arg and Met of racehorses, and this was considered to relate to an environmental effect. Moreover, our study is the first to show 3-MH in plasma of racehorses affected by environmental change.

EFFECT OF CIMATEROL ON GROWTH AND 3-METHYLHISTIDINE EXCRETION IN RATS

  • Kim, Y.S.;Lee, Y.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.4
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    • pp.313-318
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    • 1990
  • Forty-two outbred female Sprague-Dawley rats weighing 145 g were used to study the effects of a beta-agonist, cimaterol, on growth, body composition and urinary excretion of 3-methylhistidine (MH) at 3, 6 and 18 d. Cimaterol (CIM) was administered in the feed at 10 mg/kg. The growth promoting effect of CIM was most evident during the initial part of the feeding period, followed by a gradual decrease in the magnitude of the response with no significant effect at 18 d. The action of CIM was confined to skeletal and cardiac muscles with no stimulating effect on other organs. The amount of urine excretion and urinary MH excretion was reduced (p<.01) at 3 d in the CIM group. No difference was found at 6 d, followed by an increased urine excretion (p<.05) and MH excretion (p<.01) at 18 d. An inverse relationship between growth rate and urinary MH excretion suggested that the increased growth rate of CIM-fed rats during the initial part of the feeding period is primarily attributed to the decreased protein degradation rate. It was further suggested that both fractional synthesis rate and fractional degradation rate increased during the later part of the feeding period.

Effect of Orally Administered Branched-chain Amino Acids on Protein Synthesis and Degradation in Rat Skeletal Muscle

  • Yoshizawa, Fumiaki;Nagasawa, Takashi;Sugahara, Kunio
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.133-140
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    • 2005
  • Although amino acids are substrates for the synthesis of proteins and nitrogen-containing compounds, it has become more and more clear over the years that these nutrients are also extremely important as regulators of body protein turnover. The branched-chain amino acids (BCAAs) together or simply leucine alone stimulate protein synthesis and inhibit protein breakdown in skeletal muscle. However, it was only recently that the mechanism(s) involved in the regulation of protein turnover by BCAAs has begun to be defined. The acceleration of protein synthesis by these amino acids seems to occur at the level of peptide chain initiation. Oral administration of leucine to food-deprived rats enhances muscle protein synthesis, in part, through activation of the mRNA binding step of translation initiation. Despite our knowledge of the induction of protein synthesis by BCAAs, there are few studies on the suppression of protein degradation. The recent findings that oral administration of leucine rapidly reduced $N^{\tau}$-methylhistidine (3-methylhistidine; MeHis) release from isolated muscle, an index of myofibrillar protein degradation, indicate that leucine suppresses myofiblilar protein degradation. The details of the molecular mechanism by which leucine inhibits proteolysis is just beginning to be elucidated. The purpose of this report was to review the current understanding of how BCAAs act as regulators of protein turnover.

Milk Protein Production and Plasma 3-Methylhistidine Concentration in Lactating Holstein Cows Exposed to High Ambient Temperatures

  • Kamiya, Mitsuru;Kamiya, Yuko;Tanaka, Masahito;Shioya, Shigeru
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.8
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    • pp.1159-1163
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    • 2006
  • This experiment was performed to examine the influences of high ambient temperature on milk production, nutrient digestibility, energy and protein sufficiency ratio, and plasma metabolites concentration in lactating cows. In a $2{\times}2$ crossover design, four multiparous lactating Holstein cows were maintained in a chamber under treatment of constant moderate ($18^{\circ}C$) ambient temperature (MT) or high ($28^{\circ}C$) ambient temperatures (HT). The DMI and milk protein yield were significantly lower in HT (p<0.05). The milk yield, milk lactose yield, and milk SNF yield tended to be lower in HT (p<0.10). No statistical differences for 4% fat-corrected milk and milk fat yield were observed. Rectal temperatures were significantly higher in HT than MT (p<0.05). The apparent DM, OM, ether extract, CF, and ash digestibility did not differ between treatments. On the other hand, the apparent CP digestibility was increased significantly (p<0.05) and nitrogen free extract tended to increase (p<0.10) in HT. The sufficiency ratio of ME and DCP intake for each requirement tended to be lower in HT than in MT (p<0.10). Concentrations of total protein (TP), albumin, and urea nitrogen in plasma did not differ between treatments. Plasma 3-methylhistidine (3MH) concentration as a marker of myofibrillar protein degradation tended to be higher in HT (p<0.15). In conclusion, high ambient temperature was associated with a lower energy and protein sufficiency ratio, and decreased milk protein production, even though the body protein mobilization tended to be higher.