• Title, Summary, Keyword: ARTEMISIAE ARGI FOLIUM

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Effect of Water Extract from Artemisiae Argi Folium on Hepatotoxicity Caused by Acetaminophen and Acetaldehyde (Acetaminophen과 Acetaldehyde로 유발된 간세포독성에 대한 애엽 물추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.5
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    • pp.1210-1214
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    • 2008
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium (WAAF) on hepatotoxicity caused by acetaminophen (AAP) and acetaldehyde which are regarded as hepatotoxin. Artemisiae Argi Folium was known to have the antibacterial, immune-enhancing, and anticoagulative properties. In Korean Medicine, Artemisiae Argi Folium is supposed to be related with 'liver meridian' according to traditional medical theory. AAP and acetaldehyde reduce the intracellular production of hydrogen peroxide ($H_2O_2$) and nitric oxide (NO) production of human hepatocyte HepG2. The intracellular production of hydrogen peroxide ($H_2O_2$) was measured by dihydrorhodamine 123 (DHR) assay. NO production was measured with Griess test. WAAF increased the production of $H_2O_2$ and NO reduced by AAP and acetaldehyde in HepG2 cells. Therefore, It could be suggested that WAAF has the hepatoprotective activity against AAP and acetaldehyde.

Cytotoxicity and Antioxidative Activity of Artemisiae Argi Folium Alcoholic Extracts and Their Fractions (애엽(艾葉) 에탄올 및 메탄올 추출물과 용매별 분획의 세포독성과 항산화활성)

  • Lee, Kyoung-In;Cho, Joo-Hyun;Pyo, Byoung-Sik
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.6
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    • pp.421-425
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    • 2011
  • This experiment was carried out to obtain the cytotoxicity and antioxidative activity of Artemisiae Argi Folium. The total polyphenol contents in the ethyl acetate fraction of the ethanol extract and the methanol extract were 430.27mg/g and 427.84mg/g, respectively. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate fraction of the ethanol extract and the methanol extract were 32.64 ${\mu}g/m{\ell}$ and 27.70 ${\mu}g/m{\ell}$ as the same level of statistical with ascorbic acid. In the cytotoxicity measurement by MTT assay, the chloroform and hexane fraction, and each extract were exhibited higher cytotoxicity than the other fractions. In particular, the ethyl acetate fractions appeared high activity in DPPH radical scavenging ability were began to show cytotoxicity in 125 ${\mu}g/m{\ell}$. As a result, the ethyl acetate fraction of Artemisiae Argi Folium extract was the most highly active fraction in antioxidative activity. However, for the use of extracts and fractions from Artemisiae Argi Folium to related fields, the setting of appropriate concentration is required.

Study on Biological Effect of Water Extract from ARTEMISIAE ARGI FOLIUM on Mouse Macrophage Raw 264.7 Cells (마우스 대식세포(Raw 264.7)에 대한 애엽(艾葉) 물추출물의 생리활성 연구)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.4
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    • pp.815-820
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    • 2008
  • Macrophage is the important cell for the immune system. Many of herbal drugs were searched about their immune-modulating activity. The purpose of this study is to investigate the biological effect of water extract from ARTEMISIAE ARGI FOLIUM (WAAF) on mouse macrophage Raw 264.7 cells. ARTEMISIAE ARGI FOLIUM was known to have the antibacterial, immune-enhancing, and anticoagulative properties. Cytotoxicity of WAAF was verified by MTT assay. The intracellular production of hydro peroxide ($H_2O_2$) by WAAF was examined. The productions of nitric oxide (NO) and $TNF-{\alpha}$ from Raw 264.7 cell by WAAF were also examined. WAAF showed no cytotoxicity on RAW 264.7 cells for 3 hours. WAAF increased the production of $H_2O_2$ in Raw 264.7 cells. WAAF decrease the production of NO from the cells at low concentrations but increased at high concentrations. WAAF increased the production of $TNF-{\alpha}$ from the cells. Therefore, It could be suggested that WAAF has the immune-modulating effect.

Effect of Mixture of Fermented Artemisiae Argi Folium and Fermented Sophorae Radix on Hydrogen Peroxide Production within Mouse Macrophage Raw 264.7 with EtOH and Nicotine (고삼과 애엽의 발효 혼합물이 에탄올과 니코틴으로 유발된 마우스 대식세포 내 hydrogen peroxide 생성감소에 미치는 영향)

  • Park, Wan-Su;Kim, Do-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.5
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    • pp.1293-1298
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    • 2008
  • The purpose of this study is to investigate the effect of a mixture of fermented Artemisiae Argi Folium and fermented Sophorae Radix (FAS) on hydrogen peroxide production within mouse macrophage Raw 264.7 with ethanol (EtOH) and nicotine. Artemisiae Argi Folium is known to have the antibacterial, immune-enhancing properties. And Sophorae Radix is also known to have the antibacterial, anti-inflammatory, anti-allergic properties. EtOH and nicotine are the ones of toxicants which could impair immunocytes like macrophage. EtOH and nicotine reduce the intracellular production of hydrogen peroxide ($H_2O_2$) of Raw 264.7. FAS increased the production of hydrogen peroxide reduced by EtOH and nicotine within Raw 264.7. These results indicate that FAS could restore the immuno-activity of macrophage impaired by EtOH and nicotine.

Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus and Saccharomyces Cerevisiae on TNF-${\alpha}$ Production in RAW 264.7 and HepG2 Cells (유산균 발효 애엽과 효모균발효 애엽 물추출물의 종양괴사인자-알파 생성촉진효과)

  • Kim, Youn-Sub;Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.6
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    • pp.956-961
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    • 2010
  • Tumor necrosis factor-alpha (TNF-${\alpha}$) is a major mediator of immuno-inflammatory activity. The purpose of this study is to investigate whether TNF-${\alpha}$ productions of mouse macrophage RAW 264.7 and human hepatocyte HepG2 are modulated by Artemisiae argi Folium water extract (AW), Lactobacillus pentosus-fermented Artemisiae argi Folium water extract (AFL), and Saccharomyces cerevisiae-fermented Artemisiae argi Folium water extract (AFS) for 3 h of incubation. Effect of AW on cell viability of HepG2 was also investigated. TNF-${\alpha}$ productions were measured by Enzyme-Linked Immnunosorbent Assay method and cell viability was measured by MTT assay. Both AFL and AFS significantly increased TNF-${\alpha}$ productions of RAW 264.7 at the concentration of 50, 100, and 200 ${\mu}g$/mL (p<0.05). Also, AFL and AFS significantly increased TNF-${\alpha}$ productions of HepG2 at the concentration of 50, 100, and 200 ${\mu}g$/mL (p<0.05). AW significantly increased TNF-${\alpha}$ production of HepG2 at the concentration of 100 and 200 ${\mu}g$/mL (p<0.05). AW did not show any cytotoxicity on HepG2 cells for 3 h. These results suggest that AFL, AFS, and AW have the immune-enhancing property related with its increasing effect on TNF-${\alpha}$ production of macrophage and hepatocyte.

Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Gallic acid 등으로 유발된 인간 간 조직세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향)

  • Park, Wan-Su;Kim, Do-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.6
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    • pp.1379-1384
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    • 2009
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFL (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFL could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.

Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Viability of Human Hepatocyte Treated with Toxicants (EtOH 등의 독성물질에 대한 유산균발효애엽 추출물의 간세포보호효과)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.3
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    • pp.457-462
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    • 2010
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on viability of human hepatocyte HepG2 cells treated with hepatotoxicants such as EtOH, gallic acid, nicotine, acetaminophen, acetaldehyde, and lipopolysaccharide. AFL (0~400 ug/mL) was treated with EtOH, gallic acid, nicotine, acetaminophen, acetaldehyde, and lipopolysaccharide. And the viability of HepG2 cells was measured by MTT assay. AFL at the high concentration such as 400 ug/mL showed to increase significantly viabilities of HepG2 cells compared with hepatotoxicants (EtOH, gallic acid, nicotine, acetaminophen, and lipopolysaccharide) only (p<0.05). AFL could be supposed to have the hepatoprotective effect against hepatotoxicants such as gallic acid, EtOH, nicotine, acetaminophen, and lipopolysaccharide at the high concentration.

Effect of Artemisiae Argi Folium Fermented with Sacchromyces Cerevisiae on Viability of Human Hepatocyte Treated with Toxicants (EtOH 등의 독성물질에 대한 효모균발효애엽 추출물의 간세포보호효과)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.2
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    • pp.284-289
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    • 2010
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Sacchromyces cerevisiae (AFS) on viability of human hepatocyte HepG2 cells treated with hepatotoxicants such as EtOH, gallic acid, nicotine, acetaminophen, acetaldehyde, and lipopolysaccharide. AFS (0~400 ug/mL) was treated with EtOH, gallic acid, nicotine, acetaminophen, acetaldehyde, and lipopolysaccharide. And the viability of HepG2 cells was measured by MTT assay. AFS showed to increase significantly viabilities of HepG2 cells compared with hepatotoxicants (EtOH, gallic acid, nicotine, acetaminophen, and lipopolysaccharide) only (p<0.05). AFS could be supposed to have the hepatoprotective effect against hepatotoxicants such as gallic acid, EtOH, nicotine, acetaminophen, and lipopolysaccharide.

Effect of Artemisiae Argi Folium Fermented with Sacchromyces Cerevisiae on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Nicotine 등으로 유발된 인간 간조직세포 내 hydrogen peroxide 생성억제에 대한 효모균발효애엽 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.1
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    • pp.96-101
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    • 2010
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Sacchromyces cerevisiae (AFS) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFS (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFS could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.

Effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium on Hydrogen Peroxide Production of Macrophage Treated with Toxicants (EtOH 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 효모균발효애엽 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.3
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    • pp.608-612
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    • 2009
  • The effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium Water extract (AFS) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with EtOH, gallic acid, Nicotine, Acetaminophen, and Acetaldehyde was investigated through this study. AFS (0-400 ug/mL) was simultaneously treated with EtOH, gallic acid, Nicotine, Acetaminophen, and Acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS restorated the intracellular productions of hydrogen peroxide reduced by EtOH, gallic acid, Nicotine, Acetaminophen within Raw 264.7 Cells. AFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst.