• 제목, 요약, 키워드: ATP

검색결과 1,699건 처리시간 0.044초

Protein Kinase C Activates ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes

  • Kim, Na-Ri;Youm, Jae-Boum;Joo, Hyun;Kim, Hyung-Kyu;Kim, Eui-Yong;Han, Jin
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.9 no.4
    • /
    • pp.187-193
    • /
    • 2005
  • Several signal transduction pathways have been implicated in ischemic preconditioning induced by the activation of ATP-sensitive $K^+$ $(K_{ATP})$ channels. We examined whether protein kinase C (PKC) modulated the activity of $K_{ATP}$ channels by recording $K_{ATP}$ channel currents in rabbit ventricular myocytes using patch-clamp technique and found that phorbol 12,13-didecanoate (PDD) enhanced pinacidil-induced $K_{ATP}$ channel activity in the cell-attached configuration; and this effect was prevented by bisindolylmaleimide (BIM). $K_{ATP}$ channel activity was not increased by $4{\alpha}-PDD$. In excised insideout patches, PKC stimulated $K_{ATP}$ channels in the presence of 1 mM ATP, and this effect was abolished in the presence of BIM. Heat-inactivated PKC had no effect on channel activity. PKC-induced activation of $K_{ATP}$ channels was reversed by PP2A, and this effect was not detected in the presence of okadaic acid. These results suggest that PKC activates $K_{ATP}$ channels in rabbit ventricular myocytes.

Eugenol Inhibits ATP-induced P2X Currents in Trigeminal Ganglion Neurons

  • Li, Hai Ying;Lee, Byung-Ky;Kim, Joong-Soo;Jung, Sung-Jun;Oh, Seog-Bae
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.12 no.6
    • /
    • pp.315-321
    • /
    • 2008
  • Eugenol is widely used in dentistry to relieve pain. We have recently demonstrated voltage-gated $Na^+$ and $Ca^{2+}$ channels as molecular targets for its analgesic effects, and hypothesized that eugenol acts on $P2X_3$, another pain receptor expressed in trigeminal ganglion (TG), and tested the effects of eugenol by whole-cell patch clamp and $Ca^{2+}$ imaging techniques. In the present study, we investigated whether eugenol would modulate 5'-triphosphate (ATP)-induced currents in rat TG neurons and $P2X_3$-expressing human embryonic kidney (HEK) 293 cells. ATP-induced currents in TG neurons exhibited electrophysiological properties similar to those in HEK293 cells, and both ATP- and $\alpha$, $\beta$-meATP-induced currents in TG neurons were effectively blocked by TNP-ATP, suggesting that $P2X_3$ mediates the majority of ATP-induced currents in TG neurons. Eugenol inhibited ATP-induced currents in both capsaicin-sensitive and capsaicin-insensitive TG neurons with similar extent, and most ATP-responsive neurons were IB4-positive. Eugenol inhibited not only $Ca^{2+}$ transients evoked by $\alpha$, $\beta$-meATP, the selective $P2X_3$ agonist, in capsaicin-insensitive TG neurons, but also ATP-induced currents in $P2X_3$-expressing HEK293 cells without co-expression of transient receptor potential vanilloid 1 (TRPV1). We suggest, therefore, that eugenol inhibits $P2X_3$ currents in a TRPV1-independent manner, which contributes to its analgesic effect.

Paddle-wheel유형의 2차 쌓음 단위 $Zn_2(CO_2R)_4$에 기초한 2차원 아연 배위 고분자: [Zn(ATP)(DMF)] $(ATP=2-aminoterephthalate,\;H_2N-C_6H_3-1,4-(COO)_2;\;DMF\;=\;N,\;N-dimethylformamide)$ (Two-dimensional Zinc Coordination Polymer Based Paddle-Wheel Type Secondary Building Units of $Zn_2(CO_2R)_4$: [Zn(ATP)(DMF)] $(ATP=2-aminoterephthalate,\;H_2N-C_6H_3-1,4-(COO)_2;\;DMF\;=\;N,\;N-dimethylformamide)$)

  • 민동원;이희근;이순원
    • 한국결정학회지
    • /
    • v.15 no.2
    • /
    • pp.78-82
    • /
    • 2004
  • 벤젠 존재 하에서, DMF와 에탄올의 혼합 용매에서 zinc(II) nitrate $(Zn(NO_3)_2\;{\cdot}\;6H_2O)$$ATP(2-aminoterephthalate,\;H_2N-C_6H_3-1,4-(COO)_2)$의 용매열 반응으로 2차원 배위 고분자 [Zn(ATP) (DMF)] (1)이 얻어졌다 X-ray구조 결정 결과, 2개의 아연 금속과 4개의 ATP 리간드가 paddle-wheel유형의 2차 쌓음 단위들을 형성하고, 이것들은 ATP 리간드에 의해서 연결되어 2차원 4각 망을 이룬다는 것이 밝혀졌다. 아연 금속을 기준으로 각 4각형의 크기는 약$11.1\times11.1\;{\AA}$효이다. 고분자 1을 양질의 결정 상태로 얻기 위해서는 벤젠이 요구되었다.

효소법에 의한 젓갈 중의 ATP 관련물질 측정 (Enzymatic Method .for Measuring ATP Related Compounds in Jeotkals)

  • 조영제;임영선;서덕훈;김태진;민진기;최영준
    • 한국수산과학회지
    • /
    • v.33 no.1
    • /
    • pp.16-19
    • /
    • 2000
  • 젓갈 중의 ATP 관련물질의 정확한 분석방법을 확립하기 위하여, 전통적인 방법으로 멸치젓갈을 상온 ($20{\pm}2{\circ}C$)에서 90일 동안 숙성시키면서 숙성 중의 ATP 관련물질, 그리고 시판 젓갈류의 ATP 관련물질을 HPLC법과 효소법으로 측정하여 비교하였다. 효소법에 의한 멸치젓갈의 $ATP{\~}IMP$ 함량은 숙성기간에 따라서 감소하여 90일 후에는 극미량을 나타내었으며, HxR은 50일까지 $1.83 {\mu}mole/g$로 최고값을, 그리고 Hx은 $50{\~}70$일에 $5.22{\~}5.23{\mu}mole/g$로 최고값을 나타내었으며, 그 이후로 감소하였다. 요산량은 30일 후에 $0.87{\mu}mole/g$로 검출되기 시작하여 그 이후로 증가하였으며, ATP 관련물질 총량은 숙성기간이 길어짐에 따라서 감소하는 경향을 보였다. 시판 젓갈 중의 $ATP{\~}IMP$, HxR및 Hx함량은 HPLC법과 효소법에 의한 측정값이 거의 비슷하였으며, HPLC법으로는 요산이 검출되지 않았다. 한편, 효소법으로 측정한 요산량은 $0.92{\~}2.29{\mu}mole/g$ 범위로 ATP 관련물질 총량의 $14.6{\~}28.4{\%}$를 차지하였다. 이상의 결과로부터, 젓갈 중의 ATP 관련물질은 요산도 검출이 가능한 효소법으로 분석해야 할 것이다.

  • PDF

eCRM을 연계한 ATP 모델 구현에 관한 연구 (A Design of ATP Model Related eCRM)

  • 양광모;박재현;강경식
    • 한국산업경영시스템학회:학술대회논문집
    • /
    • /
    • pp.485-490
    • /
    • 2002
  • Demands of customers are being changed and varied. And in this circumstance, it become a main issue of management that the company should produce and sell products according to the customer demands. With these trends, each company has been concentrating effects on generalization of product development technique and distinction of service for customer. To fulfill these demands of customer, they need a concept of eCRM(Web based Customer Relationship Management), and go from soiling products and services, or gathering customer requests, up to the phase of solving customer's problem by real time or previous action. With the help of internet, the frequency and speed of the problem solving has improved greatly. In the Supply chain, The ATP(Available to Promise) function doesn't only give customers to conformation of delivery. It can be used by the core function with ATP rule that can reconcile supplies and demands on the supply chain. Therefore We can be acquire the conformation about on the due date of supplier by using the ATP function of management about real and concurrent access on the supply chain, also decide the affect about product availability due to forecasting or customer's orders through the ATP. In this paper, It consolidates the necessity on a ATP and analyzes data which is concerned of ATP. Under the these environments, defines the ATP rule that can improve the customer value and data flow related the eCRM and builds on a algorithm.

  • PDF

Cyclopiazonic acid 및 aflatoxin B1이 토끼의 혈소판 응집 및 ATP 방출에 미치는 영향 (Effects of cyclopiazonic acid and aflatoxin B1 on rabbit platelet aggregation and ATP release)

  • 홍충만;조명행
    • 대한수의학회지
    • /
    • v.36 no.4
    • /
    • pp.887-894
    • /
    • 1996
  • Cyclopiazonic acid(CPA) known as stimulating the release of intracellular calcium, aflatoxin $B_1(AFB_1)$ causing gastrointestinal hemorrhage frequently were used as model toxic mycotoxins in these studies. First of all, the effects of various mycotoxins on the platelet aggregation response were determined. The effects of mycotoxins on the ATP release from platelet by aggregating factors were investigated. The results and conclusions obtained from these studies are : 1) CPA promoted ADP, collagen, thrombin, A.A. and PAF-induced rabbit platelet aggregation. $AFB_1$ inhibited collagen, A.A. and PAF-induced rabbit platelet aggregation only. 2) CPA increased both aggregation and disaggregation time, whereas $AFB_1$ decreased in a dose dependent manner. 3) CPA increased ADP, thrombin, A.A. and PAF-induced ATP release. $AFB_1$ increased A.A.-induced ATP release and decreased PAF-induced release in a dose dependent manner. In conclusion, CPA promoted platelet aggregation by the increase of ATP. Antiaggregating effects of AFB1 may be due to decreases of ATP. These data provide the basis for the future study of roles of ATP release in platelet aggregation.

  • PDF

돼지 분만 시기의 조절에 관하여;IV. 자궁 평활근의 운동성에 대한 APT의 영향 (Control of Parturition Time on Pig;IV. Effect of ATP on Uterine Smooth Muscle Motility)

  • 박상은;황보원;변유성;조광제
    • 한국가축위생학회지
    • /
    • v.19 no.2
    • /
    • pp.154-162
    • /
    • 1996
  • The effcets of adenosine 5'-triphosphate(ATP) were investigated on the uterine smooth muscle motility in the pig. The results were summarized as follows: 1. The effects of the porcine uterine smooth muscle and the contractile responses increased between the concentration of ATP $10^{-5}$ and $10^{-3}$ M with a dose-dependent manner. 2. The contractile response induced by ATP($10^{-4}$ M) was not blocked by pretreatment with cholinergic receptor blocker, atropine ($10^{-6}$ M) 3. The contractile response induced by ATP ($10^{-4}$ M) was not blocked by pretreatment with $\alpha$ -adrenergic receptor blocker, phentolamine(10$^{-6}$ M) and ${\beta}$-adrenergic blocker, propranolol ($10^{-6}$ M). 4. The contractile response induced by ATP($10^{-4}$ M) was not appeared in 4Ca^{++}$ -free medium. As the concentration of $Ca^{++}$ in $Ca^{++}$ -free medium was increased, the contractile response induced by ATP ($10^{-4}$ M) was enhenced but was completely inhibited by pretreatment with $Ca^{++}$ -channel blocker, papaverine($10^{-6}$ M) or verapamil($10^{-6}$ M). From these results, it was conclued that the effects of ATP were the contraction mediated by purinergic receptor in uterine smooth muscle of pig.

  • PDF

Effect of pH on the ATP-sensitive $K^+$ Channel in Aortic Smooth Muscle Cells from Rats

  • Kim, Se-Hoon;Kim, Il-Su;Kim, Hoe-Suk;Jeon, Byeong-Hwa;Chang, Seok-Jong
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.1 no.5
    • /
    • pp.555-563
    • /
    • 1997
  • The effects of pH on $K^+$ currents were investigated in single smooth muscle cells isolated from the thoracic aorta of Wistar-Kyoto rats. Whole-cell $K^+$ currents were recorded in the conventional configuration of the voltage-clamp technique. Pinacidil (10uM) activated the whole-cell current and the pinacidil-activated current was completely inhibited by glibenclamide (10uM) , an inhibitor of ATP-sensitive $K^+$ channel ($K_{ATP}$ channel). Pinacidil-activated current was reversed at near the $K^+$ equilibrium potential. This current was time- and voltage-independent and reduced by elevating intracellular ATP. Pinacidil-activated current was reduced by lowering the external pH. However, alteration of internal pH has controversial effects on pinacidil-activated current. When the single cell was dialyzed with 0.1 mM ATP, alteration of internal pH had no effect on pinacidil-activated $K^+$ current. In the contrast, when the single cell was dialyzed with 3 mM ATP, pinacidil-activated current was increased by lowering internal pH. Our results suggest that $K^+$ channel activated by pinacidil may be $K_{ATP}$ channel and internal $H^+$ may reduce the inhibitory effect of ATP on $K_{ATP}$ channel.

  • PDF

The Effects of Intracellular Monocarboxylates on the ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes

  • Kim, Na-Ri;Han, Jin;Kim, Eui-Yong;Ho, Won-Kyung;Earm, Yung E.
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.2 no.5
    • /
    • pp.581-589
    • /
    • 1998
  • A regulating mechanism of the ATP-sensitive potassium channels $(K_{ATP}\;channels)$ is yet to fully explained. This study was carried out to investigate the effects of intracellular application of monocarboxylates (acetate, formate, lactate, and pyruvate) on $K_{ATP}$ channels in isolated rabbit ventricular myocytes. Single channel currents of $K_{ATP}$ channels were recorded using the excised inside-out or permeabilized attached (open-cell) patch-clamp technique at room temperature. Intracellular application of acetate, formate and pyruvate led to an inhibition of channel activity, whereas intracellular application of lactate increased channel activity. These effects were reversible upon washout. Analysis of single channel kinetics showed that monocarboxylates did not affect open-time constant and close-time constant. These results suggest that monocarboxylates participate in modulating $K_{ATP}$ channels activity in cardiac cells and that modulation of $K_{ATP}$ channels activity may resolve the discrepancy between the low $K_i$ in excised membrane patches and high levels of intracellular ATP concentration during myocardial ischemia or hypoxia.

  • PDF

Characterization of Mixed Apple and Carrot Retentates Using Response Surface Methodology

  • Lee, Jun-Ho
    • Preventive Nutrition and Food Science
    • /
    • v.11 no.2
    • /
    • pp.155-159
    • /
    • 2006
  • Models capable of predicting the product quality of mixed apple and carrot retentates (MACR) have been developed using response surface methodology and used to characterize the effects of processing conditions including average transmembrane pressure (ATP), temperature, and blend ratio. Color, soluble solids, total sugar, vitamin C, acidity, turbidity, and viscosity were used to assess the product quality following the ultrafiltration (UF) process. $L^*-value$ decreased with increased ATP, but the value was not affected by changes in temperature. Blend ratio also greatly influenced the $L^*-value$. Redness ($a^*-value$), on the other hand, was less affected by temperature and ATP. As the ATP and temperature increased, yellowness increased gradually. Soluble solids contents appeared to decrease gradually as the ATP increased for all blend samples, but the effect of temperature seemed to be less. Total sugar content was more affected by temperature than ATP. In general, samples containing 75% carrot had higher amounts of vitamin C regardless of processing conditions. Changes in acidity were also complex and appeared to respond to interactions among ATP, temperature, and blend ratio. Turbidity increased for all samples as both ATP and temperature increased. The higher the amount of carrot in the blend samples, the higher values for turbidity. Although the changes were small, viscosity appeared to increase as the ATP and temperature increased during UF.