• Title, Summary, Keyword: Agaricus blazei $\beta-glucan$

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Microwave Assisted Extraction of Physiologically Active Materials from Agaricus blazei Fruiting Bodies (Agaricus blazei 자실체로부터 마이크로웨이브를 이용한 생리활성물질 추출공정)

  • 최정우;류동열;홍억기;권명상;한진수;이원홍
    • KSBB Journal
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    • v.15 no.3
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    • pp.307-312
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    • 2000
  • Physiologically active material ${\beta}$-glucan extracted from mushroom has an antitumor effect. Agaricus blazei murill(A blazel) fruiting bodies were reported to contain large amounts of ${\beta}$-glucan. The purpose of this study was to maximize ${\beta}$-glucn extraction from A. blazei by microwave assisted extraction method. Optimal extraction conditions were obtained under various extraction time and applied power which was examined as 200, 500 and 650 watt. The structure and concentrations of the raffinates were analyzed by nuclear magnetic resonance spectroscopy(NMR) and infrared spectroscopy(IR) It was proved that the extraction yield of microwave assisted extraction method was higher than that of conventional solvent extraction method. It was also observed that microwave assisted extraction method requires less amounts of solvents and extraction time compared to the conventional solvent extraction method.

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Extraction and Purification of Bioactive Materials from Agaricus blazei Fruiting Bodies (아가리쿠스 버섯에서 생리활성물질의 추출 및 정제)

  • 최정우;류동열;김영기;홍억기;권명상;한진수
    • KSBB Journal
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    • v.15 no.3
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    • pp.293-298
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    • 2000
  • ${\beta}$-Glucan a kind of polysaccharide which is particularly abundant in Agaricus blazei is known as the bioactive materials especially anticancer agents. The process development of the isolation and the purification process of water soluble ${\beta}$-glucans from A. blazei was achieved. and the process operation variables were optimized. Crude polysaccharides (CR.PS) were obtained from A. blazei by hot water extraction filtration solvent precipitation dialysis and freeze drying. Neutral and acidic fraction of polysaccharides were separated from crude polysaccharides by ion chromatography and then high molecular weight and low molecular weight fraction were separated from neutral fraction by gel chromatography. Quantitative and qualitative analysis of each compounds were performed with FT-IR NMR spectroscopy. Based on these analysis the optimal conditions of temperatures operating time organic solvent volume for precipitation and dialysis time were determined.

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Enhancement of $\beta$-D-Glucans Production by Agaricus blazei Murill by Nitrogen Supplementation

  • NA JEONG-GEOL;KIM HYUN-HAN;CHUN GIE-TAEK;CHANG YONG KEUN;LEE SANG JONG;CHUNG YEON HO
    • Journal of Microbiology and Biotechnology
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    • v.15 no.6
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    • pp.1388-1391
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    • 2005
  • Temporal changes of cell growth pattern and intracellular content of $\beta$-D-glucans were investigated with off-gas data in Agaricus blazei culture where glucose was intermittently fed. It was observed that the time point of carbon source depletion coincided with the point of sudden drop in the carbon dioxide evolution rate (CER), and that the sole supplementation of glucose was not enough to maintain active cell growth and glucan content. On the other hand, when yeast extract, a typical nitrogen source, was supplemented together with glucose when the CER suddenly dropped because of carbon source depletion, an active cell growth could be maintained until the end of the culture and the glucan content did not decrease with culture time, significantly enhancing glucan productivity.

Effective Production of $\beta$-Glucan by the Liquid Cultivation of Agaricus blazei (Agaricus blazei 균사체 배양기술을 통한 효율적인 $\beta$-glucan의 생산)

  • 이승현;임환미;김태영;조남석;박준성;유연우;김무성
    • Korean Journal of Microbiology
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    • v.40 no.1
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    • pp.54-59
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    • 2004
  • $\beta$-Glucan has been efficiently produced with higher yield by the optimization of liquid cultivation conditions. The optimal composition of medium for batch culture was 5% (w/v) of glucose as a carbon source, 0.5% (w/v) of yeast and 0.5% (w/v) of malt extract as a nitrogen source, 0.1% (w/v) of $KH_2PO_4$ and 0.05% (w/v) $MgSO_4{\cdot}7H_2O$, which had been the base medium for determination of other conditions. The set-up conditions are pH 5.0, $28^{\circ}C$, 1 vvm for aeration and 300 rpm for agitation. In order to minimize the inhibition effect of glucose on the initial growth of mycelia and to maximize the production of extracellular $\beta$-glucan, we have reduced the initial glucose feed to 4% and added 2nd feed at the point of 70 hr from the initial feed. The 2nd feed was composed of glucose 3%, yeast extract 0.1 % and malt extract 0.1 %. It improved the $\beta$-glucan yield upto 5.2 g/L in comparison with 2.8 g/L resulted from batch cultivation. Moreover, the serial treatment of a cell wall lytic enzyme and bromelain to the mycelia was effective for extraction of the cell wall bound $\beta$-glucan. The yield of $\beta$-glucan extraction by the enzyme treatment was 3.5 g/L, which was almost 4 times higher than that by hot-water extraction.

Immuno enhancing and chemopreventing agent from mushroom mycelial culture (버섯균사체 배양물로부터 면역증진 기능성 소재 개발)

  • Kim, Jeong-Ok
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • pp.27-31
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    • 2007
  • This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factors such as TNF-${\alpha}$ and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which showed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR. Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors (NK. cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.

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버섯균사체 배양물로부터 면역증진 기능성 소재 개발

  • Kim, Jeong-Ok
    • Food preservation and processing industry
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    • v.6 no.2
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    • pp.11-13
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    • 2007
  • This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factor such as TNF-a and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which shoed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR, Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors(NK cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.

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Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Agaricus blazei Murill

  • Chung, Ha-Yull;Cho, Young-Jun;Kim, Tae-Wook
    • Food Science and Biotechnology
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    • v.14 no.2
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    • pp.259-262
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    • 2005
  • A fraction (CMEx-AH-${\beta}$) of water-soluble polysaccharides, showing selective antitumor activities, was isolated from the mycelia of solid cultured Agaricus blazei Murill by hot-water extraction, ethanol precipitation, and series of chromatography. Chemical characteristics of CMEx-AH-${\beta}$, were as follows: carbohydrate content, 48%; monosaccharide composition, Man:Glu:Gal (2:93:5); molecular weight, $2{\times}10^5$; uronic acid content, 6.2%. Fundamental structure of CMEx-AH-${\beta}$, was deduced as ${\beta}-(1\;{\rightarrow}\;6)$-D-glucan with ${\beta}-(1\;{\rightarrow}\;3)$-D-glucosidic side chains based on results of methylation and $^{13}C$ NMR spectroscopic analyses.

Effects of ${\beta}-Glucan$ from Agaricus blazei Murill on Blood Glucose and Lipid Composition in db/db Mice (db/db 마우스에서 아가리쿠스 버섯 ${\beta}-Glucan$이 혈당과 지질성분에 미치는 영향)

  • Choi, Jung-Mi;Koo, Sung-Ja
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1418-1425
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    • 2000
  • Obesity and diabetes mellitus are associated with common pathogenic mechanism, and ${\beta}-glucan$ of Agaricus blazei Murill is potent inhibitor of intestinal ${\alpha}-glycosidase$ and inhibit the digestion of starch and sucrose in the small intestine. In this studies, there was observed the anti-hyperglycemic effect in obese diabetic mice(C57BLKsJ db/db), which were supplied Agaricus and Acarbose for 5 weeks. In db/db mice, food intake and body weight gain were decreased significantly in Agaricus groups(p<0.05). Also these group exhibited lower fasting serum glucose level compared with control group. HbA1c level, triglyceride level, total cholesterol level, HDL cholesterol level, LDL cholesterol level and VLDL cholesterol level were lowered in db/db mice. The activity of disaccharidases on proximal and distal segments of small intestine was decreased. In conclusion, it was assumed that ${\beta}-glucan$ of Agaricus blazei Murill has anti-hyperglycemic and anti-obesitic effects by reducing food intake and body weight gain, and also decreasing serum glucose and lipid level through inhibiting the activity of small intestinal disaccharidases.

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Effects of Extrusion and Enzyme Treatment on Extraction of β-Glucan from Agaricus blazei Murill (압출성형과 효소처리가 신령버섯 β-Glucan의 추출에 미치는 영향)

  • Gil, Sun-Kook;Shin, Joong-Yup;Kang, Dae-Il;Ryu, Gi-Hyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.3
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    • pp.380-385
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    • 2016
  • This study analyzed changes in ${\beta}$-glucan content in Agaricus blazei Murill concentrates according to extrusion and extraction conditions. Screw speed and feed rate were fixed to 250 rpm, and 100 g/min, respectively. Moisture contents (20 and 30%) and barrel temperature (130 and $140^{\circ}C$) were adjusted. ${\beta}$-Glucan content of the extruded sample at a moisture content of 20% and barrel temperature of $130^{\circ}C$ was higher compared to other extrusion conditions. ${\beta}$-Glucan content of the extruded sample at a moisture content of 20% and barrel temperature of $130^{\circ}C$ treated with Rohament CL enzyme was higher compared to Viscozyme L, and Plantase TL enzyme treatments under the same extrusion conditions. In conclusion, extrusion and pretreatment with Rohament CL enzyme enhanced yield of ${\beta}$-glucan extract.

Study on biopolymer delivery system and effect of wound healing with polysaccharide extracted from Agaricus blazei Murill

  • Kim, Min-Kyoung;Lee, Hye-Young;Hong, Eock-Kee
    • 한국생물공학회:학술대회논문집
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    • pp.816-820
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    • 2005
  • Polysaccharide-based dressings have increasingly become viable alternatives to somewhat less biocompatible and often problematic cotton or viscose gauzes traditionally used for wound dressings. Polysaccharide which is particularly abundunt in Agaricus blazei Murill is known as the bioactive materials. Polysaccharide extracted from Agaricus blazei Murill enhances the recovery of the damaged epidermal tissue and the production of epithelial cell growth factors which are necessary to heal the burn and wound on the epidermal tissue. In this study, the biopolymer delivery system of polysaccharide extracted from Agaricus blazei Murill into epidermal tissue was elucidated using the skin permeating enhancer in vitro. The enhancing effects of various penetration enhancers and some other enhancers on the permeation of ${\beta}-glucan$ were evaluated using Franz diffusion cell. Permeation of polysaccharide in nomal and burn skin was compared. Polysaccharide was studied for the burn and wound healing activity in the epidermal tissue on rats.

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