• Title, Summary, Keyword: Alginate

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An Developmental Study of Artificial Skin Using the Alginate Dermal Substrate: Preliminary Report (알지네이트 진피지지체 인공피부 개발: 예비보고)

  • Park, Dae Hwan;Shin, Jeong Im
    • Archives of Plastic Surgery
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    • v.33 no.1
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    • pp.21-30
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    • 2006
  • Alginate, a polymer of guluronic and mannuronic acid, is used as a scaffolding material in biomedical applications. The research was to produce highly-purified alginate from seaweeds and to evaluate the efficacy of alginate as dermal substrate. Our alginate purification method showed a production rate as high as 25%. The purified alginate contained little polyphenol contents and endotoxin, proteins. For study of wound healing, full thickness skin defects were made on the dorsal area of the animal models. And then alginate, fibroblast-growth-factor mixed alginate, alginate-collagen complex, vaseline gauze as control were applied on the wound, respectively, and were evaluated grossly and histopathologically. For biocompatibility test, alginate and alginate-collagen complex discs were implanted on the back of Sprague-Dawly rats. Four weeks after implantation, the animals were examined immunologically against alginate and collagen. Alginate and FGF-mixed alginate, alginate-collagen complex group showed statistically higher percentage of wound contraction and wound healing than control group(p<0.05). Alginate-collagen complex group and FGF-mixed alginate group showed statistically higher percentage of wound healing than alginate group. The experiment of biocompatibility and immunologic reaction against impanted alginate or collagen needs more investigation. Highly-purified alginate from seaweeds by our purification method, showed the effect of wound healing, and addition of FGF or collagen increases the alginate's wound healing effect. It shows the possibility of alginate as a dermal substrate.

Release proporties of ovalbumin from alginate microspheres prepared using the nozzle in spray dryer system

  • Park, Jeong-Eun;Lee, Chang-Moon;Park, Hee-Jung;Kim, Gwang-Yun;Rhee, Joon-Haeng;Lee, Ki-Young
    • 한국생물공학회:학술대회논문집
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    • pp.570-573
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    • 2005
  • The spray method was chiefly used to prepare alginate microspheres. Additionally due to formation at mild conditions, the alginate microspheres were coated with chitosan. The particle size of alginate microspheres increased when the sodium alginate increased. Release pattern of OVA in alginate microspheres was evaluated at PBS buffer(pH 7.4) and HCl buffer(pH 1.2). Release rate of OVA from chitosan/alginate microsphere was also lower than that with the concentration of alginate in the microspheres, the amount of OVA released from alginate microspheres increased from alginate micorsphere. Therefore, the alginate microspheres can be prepared by spray rozzle for a protein drug delivery. OVA release from the alginate microspheres was controlled by a coating with chitosan.

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Preparation and in Vitro Release of Melatonin-Loaded Multivalent Cationic Alginate Beads

  • Lee, Beom-Jin;Min, Geun-Hong;Kim, Tae-Wan
    • Archives of Pharmacal Research
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    • v.19 no.4
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    • pp.280-285
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    • 1996
  • The sustained release dosage form which delivers melatonin (MT) in a circadian fashion over 8 h is of clinical value for those who have disordered circadian rhythms because of its short halflife. The purpose of this study was to evaluate the gelling properties and release characteristics of alginate beads varying multivalent cationic species $(Al^{+++}, \; Ba^{++}, \; Ca^{++}, \; Mg^{++}, \; Fe^{+++}, \; Zn^{++})$. The surface morphologies of Ca- and Ba-alginate beads were also studied using scanning electron microscope (SEM). MT, an indole amide pineal hormone was used as a model drug. The $Ca^{++}, \; Ba^{++}, \; Zn^{++}, \; Al^{++}\; and\; Fe^{+++}\; ions\; except\; Mg^{++}$ induced gelling of sodium alginate. The strength of multivalent cationic alginate beads was as follows: $Al^{+++}\llFe^{+++} the induced hydrogel beads were very fragile and less spherical. Fe-alginate beads were also fragile but stronger compared to Al-alginate beads. Ba-alginate beads had a similar gelling strength but was less spherical when compared to Ca-alginate beads. Zn-alginate beads were weaker than Ca- and Ba-alginate beads. Very crude and rough crystals of Ba- and Ca-alginate beads at higher magnifications were observed. However, the type and shape of rough crystals of Ba- and Ca-alginate beads were quite different. No significant differences in release profiles from MT-loaded multivalent cationic alginate beads were observed in the gastric fluid. Most drugs were continuously released upto 80% for 5 h, mainly governed by the passive diffusion without swelling and disintegrating the alginate beads. In the intestinal fluid, there was a significant difference iq the release profiles of MT-loaded multivalent cationic alginate beads. The release rate of Ca-alginate beads was faster when compared to other multivalent cationic alginate beads and was completed for 3 h. Ba-alginate beads had a very long lag time (7 h) and then rapidly released thereafter. MT was continuously released from Feand Zn-alginate beads with initial burstout release. It is assumed that the different release rofiles of multivalent cationic alginate beads resulted from forces of swelling and disintegration of alginate beads in addition to passive diffusion, depending on types of multivalent ions, gelling strength and drug solubility. It was estimated that 0.2M $CaCl_2$ concentration was optimal in terms of trapping efficiency of MT and gelling strength of Ca-alginate beads. In the gastric fluid, Ca-alginate beads gelled at 0.2 M $CaCl_2$ concentration had higher bead strength, resulting in the most retarded release when compared to other concentrations. In the intestinal fluid, the decreased release of Ca-alginate beads prepared at 0.2 M $CaCl_2$ concentration was also observed. However, release profiles of Ca-alginate beads were quite similar regardless of $CaCl_2$ concentration. Either too low or high $CaCl_2$ concentrations may not be useful for gelling and curing of alginate beads. Optimal $CaCl_2$ concentrations must be decided in terms of trapping efficiency and release and profiles of drug followed by curing time and gelling strength of alginate beads.

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The Antimutagenic Activity of Sulfated Alginate and Its Effect on the Growth of Lactobacillus acidophillus (황 흡착 알긴산의 Lactobacillus acidophillus 성장에 미치는 영향 및 항 돌연변이 작용)

  • 강경금;박난희;김정목
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.757-761
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    • 2004
  • The alginate extracted from Hizikia fusiform by the acid alkali soluble alginate (AASA) extraction method was reacted with Na$_2$SO$_4$. The amount of sulfate absorbed in the 3% alginate solution was determined about 18,435 ppm by inductively coupled plasma-atomic emission spectrometry. Both alginate and S-alginate stimulated the growth of Lactobacillus acidophilus in MRS broth and peptone water medium. The addition of S-alginate to the media showed the higher bacterial numbers than alginate supplement. There was no mutagenic activity of S-alginate in the Ames test using the Salmonella Typhimurium TA98 and TA100 strains. The S-alginate showed suppressive effect against 2-NF and MMS of mutagens.

Isolation and Characterization of Novel Alginate-Degrading Pseudoalteromonas sp. Y-4

  • Cho, Hyeon-Ah;Kim, Hyun-Woo;Kim, Young-Mog
    • Fisheries and aquatic sciences
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    • v.15 no.3
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    • pp.259-263
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    • 2012
  • To isolate an alginate-degrading bacterium, we conducted a single colony isolation using a solid medium containing alginate as the sole carbon source. A marine bacterium Y-4 capable of degrading alginate was isolated from seawater. The strain was identified to be Pseudoalteromonas sp., based on morphological, biochemical, 16S rDNA homology, and phylogenetic analyses. Moreover, Pseudoalteromonas sp. Y-4 exhibited alginate lyase activity in the presence of 4% alginate even though many known alginate-degrading bacteria degrade in the range of 0.5-1% alginate. The optimum culture conditions for the Y-4 strain were 2% alginate, pH 8.0, and 3% NaCl at $30^{\circ}C$. The highest alginate lyase activity was also observed under the same conditions. To our knowledge, this is the first reported isolation of a marine bacterium degrading high concentrations of alginate.

Effects of extracting conditions on film properties of seatangle alginate (다시마 Alginate의 추출조건이 alginate 필름의 성질에 미치는 영향)

  • You Byeong-Jin;SHIM Jae-Man;CHANG Mi-Hwa
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.5
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    • pp.664-668
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    • 1999
  • The extracting conditions of alginates from sea tangle were evaluated by measuring water vapor permeability (WVP) and tensile properties of alginate film to obtain basic data of making an edible and biodegradable film. The alginates were extracted with $1\%,\;3\%$ and $5\%$ sodium carbonate ($Na_2CO_3$) for 1, 3, 5 and 10 hours, and the alginate film was made with various plasticizers. The higher concentration of $Na_2CO_3$ solution showed the lower viscosity and polymerization degree of alginate and the film prepared with alginates having low viscosity showed the higher WVP. The extracting hours had little effect on the WVP and the elongation of alginate film, but the polymerization degree of alginates directly affected the tensile strength of the film. The addition of sorbitol and polyethylene glycol as a plasticizer lowered the WVPs and the elongation of alginate film.

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Eletron Microscopic Observation of Calcium-Acetylated Seaweed Alginate Gel

  • Jin Woo Lee
    • Journal of Life Science
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    • v.9 no.1
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    • pp.45-49
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    • 1999
  • Seaweed alginate was acetylated by activated carbon immobilized Pseudomonas syringae in a fluidized bed, up-flow reactor. The acetylation degree of seaweed alginate was about 30%. Calcium-acetylated seaweed alginate gel bead was made and compared to calcium-seaweed alginate gel bead by the scanning electron microscopy (SEM). Structural difference of two gel beads may results from increased viscosity and decreased affinity of acetylated seaweed alginate for calcium ion. On the basis of interior and exterior structure of calcium-acetylated seaweed alginate gels and property of acetylated seaweed alginate, it seems that acetylated seaweed alginate is used for the supporter for electrophoresis and packing materials for liquid chromatography and gel filtration.

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Preparation of Antibacterial Agent using Alginate and Its Antibacterial Effect (알긴산염을 이용한 항균제의 제조 및 항균효과)

  • 이학성;서정호
    • KSBB Journal
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    • v.17 no.1
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    • pp.63-67
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    • 2002
  • Silver-alginate and copper-alginate were prepared with Na-alginate extracted from marine brown algae(Sargassum fluitans). The antibacterial effect of Ag-alginate or Cu-alginate against Staphylococcus aureus and Escherichia coli was carried out by measuring optical density of liquid culture at 600 nm. The cell growth of Staphylococcus aureus and Escherichia coli was very active at pH 7, and was inhibited by adding Ag-alginate with more than 0.006 wt.% of silver content. The antibacterial effect of Ag-alginate against S. aureus and E. coli was better than that of Cu-alginate at the same metal concentration. The cell growth of S. aureus was less inhibitory than E. coli at the same concentration of Ag-alginate. The cell growth of S. aureus and E. coli was also influenced by the characteristics of counter ion of silver.

Preparation and Properties of Antimicrobial Zinc Alginate Films according to Solution Concentration (용액 농도에 따른 항균성 알긴산 아연 필름의 제조 및 그 특성)

  • Lee, Ju-Hyun;Seo, Hye-Jin;Son, Tae-Won;Lim, Hak-Sang
    • Polymer(Korea)
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    • v.37 no.6
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    • pp.677-684
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    • 2013
  • Zinc alginate films were prepared by a film maker from sodium alginate solutions of different weight ratios and then they solidified into 3 wt% content $ZnCl_2$ coagulation solution and washed and dried at a $60^{\circ}C$ oven for 20 min. The characteristics were measured by several methods (antimicrobial activity, viscosity, FTIR, TGA, SEM, EDS, contact angle, tensile strength and solubility) and the film properties were investigated. The antimicrobial test showed that the zinc alginate films result in excellent antimicrobial activity in the two strains (Klebsiella pneumonia, Staphylococcus). The surface of zinc alginate film from the solution of 9 wt% sodium alginate showed more uniform shape than any other films and the cross-section were hard and rough when the films were well-solidified by the $ZnCl_2$ solution. The tensile strength of zinc alginate films increased along with the concentration of sodium alginate solution due to the cross-linking, and the initial thermal decomposition temperature increased gradually.

Effects of Sodium Alginate and Cellulose on Fasting Plasma Lipoprotein Composition and Choelsterol Metabolism in Rats (I) (Sodium Alginate와 Cellulose 가 흰쥐의 공복 혈장 Lipoportein 조성과 콜레스테롤 대사에 미치는 영향 (I))

  • 강희정;서명자;김은희;송영선
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.6
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    • pp.879-886
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    • 1994
  • This study was carried out to determine, the effects of sodium alginate and cellulose on the plasma lipoportein composition and cholesterol metabolism inrats.Each experimental diet contained 105 sodium alginate and cellulose by weight, respectivley and rats were fed fro 4 weeks. The results obtained were as follows : The feeding of sodium alginate and cellulose decreased total plasma cholesterol slightly . total cholesterol of Chylomicron /VLDL- , LDL-fraction and liver were decreased significantly insodium alginate group. HDL-cholesterol was slightly increased in soidum alginate group. The feeding of sodium alginate significantly lowered plasma , Chylomicron VLDL-, LDL-fraction and liver TG concentrations compared with those fed fiber-free diet . The HMG-CoA reductase activity was not different among diet groups but the lowest activity was observed in sodium alginate group. The feeding of sodium alginate significantly increased fecal cholesterol , TG, and bile acid excretion . In summary , the ingestion of sodium alginate decreased cholesterol and TG concentrations of plasma and liver. This may be explained by the facts that fecal cholesterol, bile acid and TG level were increased significantly in sodium alginate group.

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