• Title, Summary, Keyword: Antibacterial effect

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Characterization of Antibacterial Activity and Synergistic Effect of Cationic Antibacterial Peptide-resin Conjugates

  • Kim, Jeong-Min;Jang, Su-Jung;Yang, Mi-Hwa;Cho, Hyeong-Jin;Lee, Keun-Hyeung
    • Bulletin of the Korean Chemical Society
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    • v.32 no.11
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    • pp.3928-3932
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    • 2011
  • We synthesized peptide-resin conjugates (1 and 2) by immobilizing ${\beta}$-sheet antibacterial peptide and ${\alpha}$ helical antibacterial peptide on PEG-PS resin, respectively. Conjugate 1 showed considerable antibacterial activity in various conditions, whereas conjugate 2 did not exhibit antibacterial activity. The growths of various bacteria were inhibited by conjugate 1 even at lower concentrations than MIC. Conjugate 1 killed bacteria at MIC and had a potent synergistic effect with current antibacterial agents such as vancomycin and tetracycline, respectively. Overall results indicate that polymer surface modification using antibacterial ${\beta}$ sheet peptide is a powerful way to prevent microbial contamination on polymer surfaces.

A STUDY ON THE ANTIBACTERIAL EFFECT OF IRRIGATION SOLUTIONS TO STREPTOCOCCI (수종 근관 세척액의 연쇄구균에 대한 항균효과)

  • Shim, Jae-Han;Im, Mi-Kyung;Han, Du-Seok
    • Restorative Dentistry and Endodontics
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    • v.17 no.1
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    • pp.115-125
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    • 1992
  • It is advisable to use irrigation solutions which have strong antibacterial effect and weak cytotoxic effect on treating root infections. Streptococci are ones of the most frequently isolated microorganisms in infected root canals. The antibacterial effect of ten irrigation solutions were investigated on S. mutans (19449), S. sanguis. )10556) and 4 streptococci isolated from saliva of healthy persons. These streptococci were exposured to irrigation solutions during 10 sec, 30 sec,. 1 min, 3 min, 5 min, 10 min, 30 min and 2hrs respectively. Four irrigation solutions which showed strong antibacterial activity were diluted to 1 : 1 to 1 : 10,000. Then they were subcultured on blood agar plate an observed after 1 day. 5.25% NaOC1 had the strongest antibacterial effect. 30% urea and normal saline showed no antibacterial effect. 50 % citric acid and 3% $H_2O_2$ showed relatively weak antibacterial effect. 15 % EDTA had very weak antibacterial effect.

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Revelation of Antibacterial Effect Against Cariogenic Bacteria in Combination with 5-Fluorocytosine and Cytosine Deaminase from Chromobacterium violaceum YK 391. (Chromobacterium violaceum YK 391의 세포내 Cytosine Deaminnse와 5-Fluorocytosine의 병용사용이 치아우식 원인세균에 대한 항균성의 발현)

  • Kim, Jung;Jung, Hyuk-Jun;Yoo, Dae-Sik
    • Microbiology and Biotechnology Letters
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    • v.31 no.3
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    • pp.292-296
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    • 2003
  • The antibacterial effect against cariogenic bacteria was evaluated in combination of 5-FC and intracellular cytosine deaminase from Chromobacterium violaceum YK 391. While S. mutans, L. parabuchneri and A. naeslundii showed antibacterial effect against 10 mM of 5-FU, S. intermedius, S. mitis, S. agalactiae, L. lactis, A. israelii, A. viscosus don't caused antibacterial effect. The addition of the cytosine deaminase and 10 mM of 5-FC to S. mutans, S. sanguis, L. brevis, L. parabuchneri, L. oris and A. naeslundii caused weakly antibacterial effect. S. sanguis caused weakly antibacterial effect against 10 mM of 5-FC. These results suggested that combination of the cytosine deaminase and 5-FC was showed the possibility to precautionary measures of dental caries.

Antibacterial effect of bamboo charcoal on Streptococcus mutans (S. mutans에 대한 대나무 숯의 항균력에 관한 연구)

  • Choi, Mi-Suk;Ahn, Kwon-Suk
    • Journal of Korean society of Dental Hygiene
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    • v.14 no.1
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    • pp.95-100
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    • 2014
  • Objectives : The purpose of the present study was to investigate the effect of bamboo charcoal on Streptococcus mutans which is one of the most important causative agents of dental caries. Methods : S. mutans was incubated with or without bamboo charcoal and then changes were observed in its cell viability and antibacterial effect. Oral epithelial cells viabillity(human gingival fibroblast, HGF) was performed using MTT assay. Antibacterial effect was analyzed using a dilution plating method and agar diffusion method. Results : Oral epithelial cells, human gingival fibroblast (HGF) showed a tendency to increase in bamboo charcoal treatment solution concentrations(0.5, 1, 2, 3, 5, 10%). The bamboo charcoal had an antibacterial effect on S. mutans. Antibacterial effect of bamboo charcoal for the bacterium was 58%. Charcoal concentration of 2% and 5% in the inhibition zone showed a minimal growth, but the concentration of 10% bamboo charcoal in inhibition zone revealed a conspicuous antibacterial activity. Conclusions : Overall results suggested that the bamboo charcoal proved to be bactericidal effect on S. mutans.

The Antibacterial Activity and Deodorization of Fabrics Dyed with Lithospermi Radix Extract (자초 추출액을 이용한 염색직물의 항균성 및 소취성)

  • 박영희;남윤자
    • Journal of the Korean Society of Clothing and Textiles
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    • v.27 no.1
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    • pp.60-66
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    • 2003
  • This study shows the colorfastness, antibacterial activity, and deodorizing effect about cotton and silk fabrics dyed with Lithospermi Radix Extract. In the case of colorfastness, the light colorfastness showed the lowest grade, the other colorfastness was relatively good. However there are some differences according to fabrics and mordants. In the case of antibacterial activity to Staphylococcus aureus, the cotton fabrics had the most antibacterial effect at the dyed fabrics that had been mordanted SnC1$_2$ㆍ2$H_2O$, and the silk fabrics had the most antibacterial effect at the dyed fabrics that had been mordanted A1($CH_3$COO)$_3$. In the case of deodorizing effect, the cotton fabrics showed the most deodorizing effect at the dyed fabrics that had been mordanted SnC1$_2$ㆍ2$H_2O$, and the silk fabrics showed the comparatively high antibacterial effect at the dyed fabrics that had been mordanted both SnCl$_2$ㆍ2$H_2O$ and Al($CH_3$COO)$_3$.

A Study on Antibacterial Assesment of High Efficiency Antibacterial Air Filter (고효율 항균 필터의 항균력 평가에 관한 연구)

  • 권혁구;정진도;류해열;정우성;박덕신
    • Proceedings of the KSR Conference
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    • pp.549-554
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    • 2002
  • Recently, interest in hygiene has been arouse in the health care field. Consequently, Filters with antibacterial agent applied to improve air quality by sterilizing bacteria, fungi, etc. We actually installed antibacterial air filter containing 2.5wt%Ag zeolite on the air intake route to air-conditioner in passenger cu, and evaluated filter's performance on antibacterial effect. By the microbe liquid spattering method, we found that the antibacterial air filter has notably sufficient antibacterial efficiency against standard strains and wild type strains. Antibacterial effect was observed at whole area of filter media by zone of inhibition test. The evaluation of microbe quantity was conducted through mixing dilution plate culture method. In comparison with ordinary filter, the amount of germs attached on antibacterial air filters was larger. The amount of germs attached on ordinary filters was very small since ordinary filters contained less dust. In comparison in antibacterial air filter with thickness, the amount of germs attached on 9mm filter was smaller than that of on 6mm filter. i.e. thicker filter, superior efficiency.

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Synergistic Antibacterial Effect and Antibacterial Action Mode of Chitosan-Ferulic Acid Conjugate against Methicillin-Resistant Staphylococcus aureus

  • Eom, Sung-Hwan;Kang, Shin-Kook;Lee, Dae-Sung;Myeong, Jeong-In;Lee, Jinhwan;Kim, Hyun-Woo;Kim, Kyoung-Ho;Je, Jae-Young;Jung, Won-Kyo;Kim, Young-Mog
    • Journal of Microbiology and Biotechnology
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    • v.26 no.4
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    • pp.784-789
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    • 2016
  • We evaluated the synergistic antibacterial effect in combination with the chitosan-ferulic acid conjugate (CFA) and β-lactam antibiotics, such as ampicillin, penicillin, and oxacillin, against methicillin-resistant Staphylococcus aureus (MRSA) using fractional inhibitory concentration (FIC) indices. CFA clearly reversed the antibacterial activity of ampicillin, penicillin, and oxacillin against MRSA in the combination mode. Among these antibiotics, the combination of oxacillin-CFA resulted in a ΣFICmin range of 0.250 and ΣFICmax of 0.563, suggesting that the oxacillin-CFA combination resulted in an antibacterial synergy effect against MRSA. In addition, we determined that CFA inhibited the mRNA expression of gene mecA and the production of PBP2a, which is a key determinant for β-lactam antibiotic resistance, in a dose-dependent manner. Thus, the results obtained in this study supported the idea on the antibacterial action mechanism that oxacillin will restore the antibacterial activity against MRSA through the suppression of PBP2a production by CFA.

A Study of high Efficiency anti-Bacteria Filters for Passenger Car (객차용 고효율 항균 기능성 필터에 관한 연구)

  • 정우성;박덕신;정진도;류해열
    • Proceedings of the KSR Conference
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    • pp.975-980
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    • 2002
  • We actually installed antibacterial air filter on the air intake route to air conditioner in passenger car and evaluated filters performance on antibacterial effect. And also, antibacterial effect was observed at whole are of filter media by zone of inhibition test. In the evaluation of antibacterial test, it was shown that the air filter has notably sufficient antibacterial efficiency against ordinary filter.

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Simultaneous Determination of Benzoic Acid, Caffeic Acid and Chlorogenic Acid in Seeds of Eriobotrya japonica and their Antibacterial Effect

  • Jeong, Jun-Mo;Lee, Kyoung-In;Kim, Sun-Min
    • Journal of Applied Biological Chemistry
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    • v.57 no.1
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    • pp.89-93
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    • 2014
  • We aim to develop a simple method for simultaneous and quantitative determination of benzoic acid, caffeic acid and chlorogenic acid in seeds of Eriobotrya japonica. In addition, antibacterial effect of these three phenolic acids was examined. A basic method is performed on the high performance liquid chromatography system coupled to an UV-detector (230 nm) and reverse phase C-18 column ($4.6{\times}150mm$, $5{\mu}m$). Each phenolic acid was confirmed via liquid chromatography-mass spectrometry (MS)/MS system under the multiple-reaction monitoring with negative-ion electrospray ionization (ESI(-)) mode. It is demonstrated that the method was could be applied to samples for an analytical study of the phenolic acids. On the other hand, three phenolic acids in seeds of E. japonica exhibited antibacterial effect against several pathogenic bacteria. Of these, benzoic acid was found to have stronger antibacterial effect.

Photodecomposition Effect of Metal doped $Ti0_2$ System (I)

  • Jin, Eui;Lee, Kang-Hyeob;Kim, Young-Soon;Min, Tae-Jin;Yu, Kook-Hyun
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.403-405
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    • 2002
  • To study photocatalytic mechanism of metal doped $Ti0_2$, we investigated photodecomposition effect, photocurrent effect and antibacterial effect. When aluminium content was 2 wt %, photodecomposition effect was better than the others. Silver doped thin films had high photocurrent efficiency and antibacterial effect. This reactions were caused by dissolved oxygen in solution and oxygen adsorbed on surface of thin films.

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