• Title, Summary, Keyword: Arabidopsis thaliana

Search Result 311, Processing Time 0.044 seconds

Cloning and Nucleotide Sequencing of a Partial Glutamate Decarboxylase Gene from Arabidopsis thaliana cDNA Library (애기장대 cDNA library로부터 Glutamate Decarboxylase 유전자의 부분 클로닝 및 서열분석)

  • 오석흥;최원규;최동성
    • KSBB Journal
    • /
    • v.16 no.1
    • /
    • pp.36-40
    • /
    • 2001
  • In order to study the molecular mechanism of $\gamma$-aminobutyric acid (GABA) production in plants, we cloned and sequenced a partial glutamate decarboxylase (GAD) cDNA from the Arabidopsis thaliana cDNA library, using primers targeted at highly conserved sequences of the petunia GAD gene. The cDNA fragment was inserted into TA cloning vector with T7 promoter and the recombinant plasmid obtained was used to transform E. coli. The plasmid DNA purified from the transformed E. coli was digested with EcoRI and the presence of the insert was confirmed. Nucleotide sequence analysis showed that the fragment is a partial Arabidopsis thaliana GAD gene and that the sequence showed 98% and 78% identity to the region of the putative Arabidopsis thaliana GAD sequences deposited in GenBank, Accession nos: U46665 and U10034, respectively. The amino acid sequence deduced from the partial Arabidopsis thaliana GAD gene showed 99% and 91% identities to the GAD sequences deduced from the genes of the U46665 and U10034, respectively. The partial cDNA sequence determined may facilitate the study of the molecular mechanism of GABA metabolism in plants.

  • PDF

Epigenetic aspects of telomeric chromatin in Arabidopsis thaliana

  • Lee, Won Kyung;Cho, Myeon Haeng
    • BMB Reports
    • /
    • v.52 no.3
    • /
    • pp.175-180
    • /
    • 2019
  • Telomeres are nucleoprotein complexes at the physical ends of linear eukaryotic chromosomes. They protect the chromosome ends from various external attacks to avoid the loss of genetic information. Telomeres are maintained by cellular activities associated with telomerase and telomere-binding proteins. In addition, epigenetic regulators have pivotal roles in controlling the chromatin state at telomeres and subtelomeric regions, contributing to the maintenance of chromosomal homeostasis in yeast, animals, and plants. Here, we review the recent findings on chromatin modifications possibly associated with the dynamic states of telomeres in Arabidopsis thaliana.

Activities of Sulfhydryl-Related and Phenylpropanoid-Synthesizing Enzymes during Leaf Development of Arabidopsis thaliana

  • Sa, Jae-Hoon;Park, Eun-Hee;Lim, Chang-Jin
    • BMB Reports
    • /
    • v.31 no.6
    • /
    • pp.554-559
    • /
    • 1998
  • Activities of glutathione- and thioredoxin-related enzymes and phenylpropanoid-synthesizing enzymes were measured and compared in the developing leaves of Arabidopsis thaliana. Phenylalanine ammonia-lyase activity is maximal in the leaves of 2-wk-grown Arabidopsis. Tyrosine ammonia-lyase activity is maximal in the leaves of 3-wk-grown and 4-wk-grown Arabidopsis. Activity of thioitransferase, an enzyme involved in the reduction of various disulfide compounds, is higher in younger leaves than in older ones. A similar pattern was obtained in the activity of thioredoxin, a small protein known as a cofactor of ribonucleotide reductase and a regulator of photosynthesis. Activity of glutathione reductase is also higher in the younger leaves. Malate debydrogenase activity remains relatively constant during the development of Arabidopsis leaves. The results offer preliminary information for further approach to elucidate the mechanism of growth-dependent variations of these enzymes.

  • PDF

Glutathione S-Transferase Activities of S-Type and L-Type Thioltransferases from Arabidopsis thaliana

  • Cho, Young-Wook;Park, Eun-Hee;Lim, Chang-Jin
    • BMB Reports
    • /
    • v.33 no.2
    • /
    • pp.179-183
    • /
    • 2000
  • The glutathione S-transferase (GST) activities of S-type and L-type thioltransferases (TTases), which are purified from the seeds and leaves of Arabidopsis thaliana, respectively, were identified and compared. The S-type and L-type TTases showed $K_m$ values of 9.72 mM and 3.18mM on 1-chloro-2,4-dinitrobenzene (CDNB), respectively, indicating the L-type TTase has higher affinity for CDNB. The GST activity of the L-type TTase was rapidly inactivated after being heated at $70^{\circ}C$ or higher. The GST activity of the S-type TTase remains active in a range of $30-90^{\circ}C$. $Hg^{2+}$ inhibited the GST activity of the S-type TTase, whereas $Ca^{2+}$ and $Cd^{2+}$ inhibited the GST activity of the L-type TTase. Our results suggest that the GST activities of two TTases of Arabidopsis thaliana may have different catalytic mechanisms. The importance of the co-existence of TTAse and GST activities in one protein remains to be elucidated.

  • PDF

Proteome Analysis of Vernalization-Treated Arabidopsis thaliana by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

  • Cho, Mi-Ran;Lee, Kyung-Hyeon;Hyun, You-Bong;Lee, Il-Ha;Kim, Hie-Joon
    • Bulletin of the Korean Chemical Society
    • /
    • v.28 no.3
    • /
    • pp.427-431
    • /
    • 2007
  • In order to gain insight into the molecular changes at the protein level in plants exposed to low temperature for a long period of time (vernalization), proteome analyses of vernalization-treated Arabidopsis thaliana have been carried out by two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Fourteen proteins including ATP binding/GTP binding/translation elongation factor and glycine-rich RNA-binding protein 7 (GRP7) showed differential expression in vernalization-treated Arabidopsis thaliana. GRP7 showed the most dramatic increase in expression suggesting its involvement in response to vernalization treatment.

Investigation of Splicing Quantitative Trait Loci in Arabidopsis thaliana

  • Yoo, Wonseok;Kyung, Sungkyu;Han, Seonggyun;Kim, Sangsoo
    • Genomics & Informatics
    • /
    • v.14 no.4
    • /
    • pp.211-215
    • /
    • 2016
  • The alteration of alternative splicing patterns has an effect on the quantification of functional proteins, leading to phenotype variation. The splicing quantitative trait locus (sQTL) is one of the main genetic elements affecting splicing patterns. Here, we report the results of genome-wide sQTLs across 141 strains of Arabidopsis thaliana with publicly available next generation sequencing datasets. As a result, we found 1,694 candidate sQTLs in Arabidopsis thaliana at a false discovery rate of 0.01. Furthermore, among the candidate sQTLs, we found 25 sQTLs that overlapped with the list of previously examined trait-associated single nucleotide polymorphisms (SNPs). In summary, this sQTL analysis provides new insight into genetic elements affecting alternative splicing patterns in Arabidopsis thaliana and the mechanism of previously reported trait-associated SNPs.

Cytohistological Study of Abnormal Cell Division of Arabidopsis Stem Infected with Geminivirus (Geminivirus에 감염된 Arabidopsis 줄기의 이상세포분열에 관한 세포조직학적 연구)

  • 박종범;이석찬
    • Korean Journal of Plant Tissue Culture
    • /
    • v.25 no.3
    • /
    • pp.153-158
    • /
    • 1998
  • The internal structures of Arabidopsis thaliana infected with beet curly top virus (BCTV) were studied by light microscopy. Hyperplasia was observed in the inflorescence stems of Arabidopsis thaliana ecotype Sei-O at 2 weeks after BCTV-Logan inoculation and callus was induced on symptomatic tissues at 4 weeks after virus inoculation. The infection processes were revealed as follows: hyperplasia of phloem tissue, necrosis of hyperplastic phloems, lacuna formation of necrotic tissues, elongation and enlargement of cortex and epidermal cells surrounding the lacuna formed phloem tissues, induction of cell division in the enlarged cortex and epidermal cells, and induction of callus tissue. Callus formation on Arabidopsis was caused by the virus infection, and virus inclusion body was observed in both phloem and callus tissue by azure-A staining.

  • PDF

Overexpression of the Metal Transport Protein1 gene (MTP1) in Arabidopsis Increased tolerance by expression site (금속전달 유전자(MTP1)의 과발현 애기장대에서 발현 위치에 따른 내성 증가 연구)

  • Kim, Donggiun
    • The Journal of the Convergence on Culture Technology
    • /
    • v.5 no.3
    • /
    • pp.327-332
    • /
    • 2019
  • Today's scientists try to remove heavy metals with many new technologies such as phytoremediation. One of the best cutting edge technologies is developing transgenic plants to remove certain heavy metal in soil. I constructed the transformation vector expressing T. goesingense Metal Transport Protein1 gene and TgMTP1: GFP genes. The transgenic plants were selected and confirmed the transformed genes into Arabidopsis thaliana genome. Expression was confirmed in several parts in Arabidopsis cells, tissues and organs. When TgMTP1 overexpressing Arabidopsis thaliana were subjected, transgenic plants showed higher heavy metal tolerance than non-transgenic. For further study I selected the transgenic plant lines with enhanced tolerance against four different heavy metals; Zn, Ni, Co, Cd. The accumulation of these metals in these plants was further analyzed. The TgMTP1 overexpressing Arabidopsis thaliana plant of selected lines are resistant against heavy metals. This plant is characterized by the expression of the MTP1 gene accumulating heavy metal in the vacuole and being simultaneously expressed on the plasma membrane. In conclusion, these plants may be used in plant purification applications, and as a plant with increased tolerance.

Identification of Three Genetic Loci Required for Progression of Leaf Senescence in Arabidopsis thaliana

  • Oh, Sung-Aeong;Park, Joon-Hyun;Lee, Gyu-In;Paek, Kyung-Hee;Park, Soon-Ki;Nam, Hong-Gil
    • Proceedings of the Botanical Society of Korea Conference
    • /
    • /
    • pp.19-25
    • /
    • 1996
  • Three key genetic loci required for proper progression of leaf senescence were identified in Arabidopsis thaliana. Mutations in these loci cause delay in all senescence symptoms examined, including both anabolic and catabolic activities, during natural senescence and upon artificial senescence induced by various senescence-inducing treatments. The result provides a decisive evidence that leaf senescence is a genetically programmed phenomenon controlled by several monogenic loci in Arabidopsis thaliana. The result further indicates that leaf senescence caused by various senescence signals occurs, at least in part, through common pathways in Arabidopsis and that the threed genetic loci function at the common steps.

  • PDF