• Title, Summary, Keyword: Arctium lappa

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Effects of Arctium lappa Linne on the DNFB-induced allergic dermatitis (우방자(牛蒡子)의 아토피 피부염에 대한 효과)

  • Kim, Gae-Eun;Jung, Sung-Ki;Jung, Hee-Jae;Jang, Hyung-Jin
    • The Journal of Internal Korean Medicine
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    • v.31 no.2
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    • pp.201-211
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    • 2010
  • Objectives : Atopic dermatitis is a chronic inflammatory skin disease which is characterized by severe pruritis, erythema, edema, effusion and scabs. The aim of this study was to understand effects of Arctium lappa Linne, which is well known for its efficacy for various skin diseases, on atopic dermatitis Methods : We conducted this experiment using the DNFB-induced NC/Nga mice. After two weeks of applying DNFB to NC/Nga mice, severe symptoms of atopic dermatitis occurred. We divided the mice into three different groups: a control group which was given no treatment at all, a group treated with dexamethasone (1mg/kg), and another group treated with Arctium lappa Linne (300mg/kg). After one week of treatment, results were recorded according to their improvement on skin, itching behavior, IL-4, and INF-$\gamma$ measurement, which is a significant criterion for diagnosing atopic dermatitis. Results : Itching behavior showed significant improvement in the Arctium lappa Linne group. However Arctium lappa Linne failed to reduce IL-4 and INF-$\gamma$ count. Also there was no satisfying improvement on AD-like skin lesions on the rostral back of the NC/Nga mice treated with Arctium lappa Linne. Conclusions : Arctium lappa Linne showed improvement in itching behavior in NC/Nga mice induced by DNFB. Arctium lappa Linne showed neither significant improvement on skin lesions nor in IL-4 and INF-$\gamma$ measurement.

Antioxidative and Antimutagenic Effects of $Arctium$ $lappa$ Ethanol Extract (우엉 에탄올 추출물의 항산화활성과 항돌연변이 효과)

  • Lee, Mee-Sook
    • The Korean Journal of Food And Nutrition
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    • v.24 no.4
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    • pp.713-719
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    • 2011
  • The antioxidant activities of the ethanol extract of Arctium lappa were assessed by measuring the 1,1-diphenyl-2-picrylhydrazyl( DPPH) radical scavenging effect, inhibition of $Fe^{2+}$-induced lipid peroxidation, inhibition of malondialdehyde(MDA)-bovine serum albumin(BSA) conjugation reaction and antimutagenic capacities using the Ames test. The DPPH radical scavenging activity and inhibition of $Fe^{2+}$-induced lipid peroxidation of the $Arctium$ $lappa$ ethanol extract significantly increased in a dose-dependent manner. In the radical scavenging assay using DPPH, the $IC_{50}$ of the Arctium lappa extract was 296 ${\mu}g$/assay(1.29 mg of dry sample). In addition, the $IC_{50}$ in the inhibition of $Fe^{2+}$-induced lipid peroxidation was 1,759 ${\mu}g$/assay(7.65 mg of dry sample). This extract also significantly inhibited the MDA-BSA conjugation reaction with an $IC_{50}$ of 57.58 mg/assay(250 mg of dry sample). However, no inhibitory effects against the direct and indirect mutagenicities in $Salmonella$ Typhimurium TA98 and TA100 were observed. Based on these results, the ethanol extract of $Arctium$ $lappa$ was shown to display considerable antioxidative activities.

Antioxidant and Anti-Obesity Activity of Ethanol Extracts from Fermented Arctium lappa L. (발효 우엉 에탄올 추출물의 항산화 및 항비만 활성)

  • Kim, Mi Hyun;Kim, Jong Gyu;Choi, Jae Hong
    • The Korean Journal of Food And Nutrition
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    • v.28 no.5
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    • pp.752-758
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    • 2015
  • This study was conducted to investigate the antioxidant and anti-obesity activity of ethanol extracts from fermented Arctium lappa L. Arctium lappa fermented twice with Phellinus linteus ($2^{nd}$ FA) showed more DPPH radical scavenging activity than non-fermented Arctium lappa (NFA), and Arctium lappa fermented once ($1^{st}$ FA) at a concentration of 62.5 ppm. The $2^{nd}$ FA showed the highest level of ABTS radical scavenging activity at concentration range of 31.5~125 ppm. The ABTS free radical scavenging activities of $1^{st}$ FA and $2^{nd}$ FA were similar to that of BHA, a synthetic antioxidant, at a concentration of 250 ppm. The total polyphenol content of $2^{nd}$ FA was higher than those of NFA and $1^{st}$ FA. The flavonoid content was significantly increased in $1^{st}$ FA and $2^{nd}$ FA than NFA. During adipocyte differentiation, lipid accumulation in 3T3-L1 cells was significantly decreased in the order of NFA, $1^{st}$ FA, and $2^{nd}$ FA at all concentrations. In conclusion, the antioxidant and anti-obesity activities of A. lappa were increased depending on the degree of fermentation. It is suggested that fermented Arctium lappa, especially $2^{nd}$ FA, could be used as a natural ingredient for functional foods and medicine.

Antioxidative Activity and Tyrosinase Inhibitory Activity of the Extract and Fractions from Arctium lappa Roots and Analysis of Phenolic Compounds (우엉 뿌리 추출물의 항산화 및 Tyrosinase 저해 활성과 Phenolic Compound 분석)

  • Im, Do Youn;Lee, Kyoung In
    • Korean Journal of Pharmacognosy
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    • v.45 no.2
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    • pp.141-146
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    • 2014
  • In this study, we investigated on antioxidative activities and tyrosinase inhibitory activities of methanol extract and its fractions from roots of Arctium lappa. The total phenolic compound and flavonoid content of the ethylacetate fraction was found to be 818.29 mg/g and 360.59 mg/g as the highest content. In the measurement of DPPH radical scavenging ability and tyrosinase inhibitory activity, the ethylacetate fraction was higher than the other fractions and the extract. In addition, comparative analysis of phenolic compounds by liquid chromatography-mass spectrometry (MS)/MS system under the multiple-reaction monitoring (MRM) with negative-ion electrospray ionization mode. The main phenolic compounds in the extract and fractions of roots from Arctium lappa were cynarin and chlorogenic acid. The main phenolic compound of the ethylacetate fraction was cynarin. n-Butanol fraction had a significantly higher chlorogenic acid content than other samples. In conclusion, DPPH radical scavenging ability and tyrosinase inhibitory activity of the cynarin-riched ethylacetate fraction showed the highest activity.

Removal Efficiency of odor substance Using Arctium Lappa Root extract (우엉발효추출물을 이용한 생활악취물질 제거효율 조사)

  • Lee, Dong-Sub;Park, Jin-Sik
    • Journal of Environmental Science International
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    • v.23 no.11
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    • pp.1953-1961
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    • 2014
  • Increasing public concerns over odors and air regulations necessitates the remediation of a wide range of odor substances for industrial and indoor purpose. Currently, the wet scrubbing technique by neutralization using oils is being used to treat odor substances such as Ammonia($NH_3$), Methyl Mercaptan($CH_3SH$), Hydrogen Sulfide($H_2S$), Methylamine($CH_3NH_2$), Acetaldehyde($CH_3CHO$). The chemical analysis is performed to analyze the composition of an Arctium Lappa Root extract by VOC analyzer(Phocheck 5000Ex, ION SCIENCE co.) The objectives of the this study are to clarify the possibility of the neutralization of odors sprayed in the fixed bed and determine the removal efficiencies for different input odor concentrations. It is found that Ammonia($NH_3$), Methyl Mercaptan($CH_3SH$), Hydrogen Sulfide($H_2S$), Methylamine($CH_3NH_2$), Acetaldehyde($CH_3CHO$) are significantly removed and their removal efficiencies are higher than 98%. The kitchen detergent with Arctium Lappa Root extract showed excellent removal efficiencies of odor substances and high possibility for the development of kitchen detergent with odor removal.

Evaluation of Pharmacognostical Characters and Comparative Morphoanatomical Study of Saussurea costus (Falc.) Lipchitz and Arctium lappa L. Roots

  • Pandey, Madan Mohan;Rastogi, Subha;Rawat, Ajay Kumar Singh
    • Natural Product Sciences
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    • v.13 no.4
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    • pp.304-310
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    • 2007
  • Saussurea costus (Falc.) Lipchitz syn S. lappa C. B. Clarke (commonly known as 'Kuth') belonging to the family Asteraceae is a well known medicinal plant which finds wide usage in different indigenous systems of medicine of India, China, Korea & Tibet. In different folk medicines the roots of S. costus are used to treat various disorders like ulcer, stomachache, malaria, leprosy, dysentery and toothache. However due to over exploitation, it has become endangered and has become the concern of different governmental bodies in India. The increasing demand of this endangered Himalayan species has resulted in a situation where it is often substituted, knowingly or unknowingly, by other morphologically similar species. Arctium lappa, belonging to the same family, is one such plant that has often been found to be present in the market samples of 'Kuth'. The present study was thus carried out and morphoanatomical characters, physicochemical as well as chemical parameters were developed for proper identification of roots of S. costus and its differenciation from A. lappa as well as authentication of the commercial market samples. The detailed morphoanatomical studies revealed that roots of S. costus can be distinguished from A. lappa on the basis of some important microscopial characters eg. the schizogenous resin ducts observed in roots of S. costus, were absent in A. lappa.. Besides, the HPTLC fingerprint profile showed a distinct band at Rf. 0.72 in S. costus, which was totally absent in A. lappa and a band at $R_f$ 0.64 in A. lappa which was absent in S. costus Chlorogenic acid, used as a chemical marker for HPTLC analysis, was estimated to be 0.077% in S. costus as compared to 0.107% in A. lappa. Thus these detailed pharmacognostical parameters can be successfully used to distinguish between roots of S. costus and A. lappa.

Major Characteristics of Burdock(Arctium lappa L.) Native to Yeong-Narn Region (영남지역 재래종 우엉(Arctium lappa L.)의 주요특성)

  • 이중환;임재하;정종도;서동환
    • Korean Journal of Plant Resources
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    • v.16 no.1
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    • pp.8-14
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    • 2003
  • This study was aimed to obtain basic information on the characteristics of local cultivars of burdock(Arctium lappa L.) grown in Yeong-Nam region. The characteristics of seeds were shown a remarkable diversity among the local cultivars. Germination rate was the highest at 25$^{\circ}C$ and Munkyong' as the highest germination rate(42.2%) at l0$^{\circ}C$ respectively. In Youchon' and 'Chilgok', about 10% was bolted but the other was rarely or none. The range of root length was 62∼77cm and lateral root number was maximum 5.5('Koryong') and minimum 0.3('Songju). 'Chongdo I' contents 14.2mg/100g of tannin in root and 'Kyongsan', 'Koryong', 'kyongiu',’ Kumi' and 'Haman' taste not bitter in leaves.

Purification and Characterization of Polyphenol Oxidase from Burdock (Arctium lappa L.) (우엉(Arctium lappa L.) 뿌리 Polyphenol Oxidase의 부분정제 및 특성)

  • Lim, Jeong-Ho;Jeong, Moon-Cheol;Moon, Kwang-Deog
    • Korean Journal of Food Preservation
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    • v.12 no.5
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    • pp.489-495
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    • 2005
  • Polyphenol oxidase (PPO) from Burdock (Arctium lappa L.) was purified and characterized. Purification of polyphenol oxidase was achieved by ammonium sulfate precipitation, Phenyl-sepharose CL-4B hydrophobic chromatography and Sephadex G-100 gel filtration chromatography. The molecular mass of the purified PPO was estimated to be 30 kDa by SDS polyacrylamide gel electrophoresis. In a substrate specificity, maximum activity was achieved with chlorogenic acid, followed by catechol and catechin. Whereas, there was low activity with hydroquinic acid, resorcinol or tyrosine. The optimum pH and temperature for enzyme activity were 7.0 and 35$\circC$ with catechol, respectively. The enzyme was most stable at pH 7.0 while unstable at acidic and alkaline pH. The enzyme was stable when heated to 40$\circC$. But heating at 50$\circC$ for more than 30 min caused 50% loss of activity. Ascorbic acid, L-cystein and $Cu^{2+}$ inhibited the activity of pholyphenol oxidase.

Antioxidant Effect of Traditional food Ingredient (전통 식재료의 항산화 효과)

  • Lee, Jae-Hyeok;Park, Jeong-Sook
    • Journal of the Korea Convergence Society
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    • v.11 no.2
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    • pp.309-314
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    • 2020
  • This study was designed to investigate the antioxidant effects of 10 kinds of medicinal plants and vegetable extracts and total extracts. The cytotoxicity was measured by MTS assay, and the antioxidant activity was measured by DPPH free radical scavenging activity and Riboflavin-derived superoxide inhibitory activity (SQA). As a result, cytotoxicity was safe for all 10 medicinal plants, vegetable extracts and total extracts. DPPH free radical scavenging ability was observed in Cinnamomum cassia Blume, Eugenia caryophyllata Thunb. Arctium lappa, Total extract was excellent, and Riboflavin-derived superoxide inhibitory activity (SQA) was found in Cinnamomum cassia Blume, Arctium lappa, Prunus mume Sieb. et Zucc., Excellent, but total extract showed the best antioxidant effect. As a result of comparing the antioxidant effects of medicinal plants and vegetables using traditional ingredients, the antioxidant activity was increased when used as a mixture than when used alone. It is considered that it can be used as an antioxidant functional material, and it is expected to be of value when developing antioxidant material in the future.

Inhibitory Constituents of LPS-induced Nitric Oxide Production from Arctium lappa

  • Park, So-Young;Hong, Seong-Su;Han, Xiang-Hua;Ro, Jai-Seup;Hwang, Bang-Yeon
    • Natural Product Sciences
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    • v.11 no.2
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    • pp.85-88
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    • 2005
  • The methanolic extract from the seeds of Arctium lappa was found to inhibit the LPS-induced nitric oxide (NO) production in murine macrophage RAW264.7 cells. Bioassay-guided fractionation of a methylene chloride soluble fraction led to the isolation of three lignan compounds, arctiin (1), arctigenin (2), and lappaol B (3). Their structures were elucidated by UV, IR, MS, and NMR data, as well as by comparison with those of the literatures. Arctigenin (2) and lappaol B (3) had an iNOS inhibitory activity with $IC_{50}$ values of 12.5 and $25.9\;{\mu}M$, respectively.