• Title, Summary, Keyword: Artemisia Capillaris Thunberg

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Effect of Artemisia Capillaris Thunberg EtOH Ext. on Lowering Lipid, Anti-oxidation and Concentration of Plasma Inflammatory Mediators Using Rats Fed on High-oxidized Fat (인진호(茵蔯蒿) 추출물이 과산화지질 투여한 쥐의 지질강하, 항산화효과 및 염증매개물질의 생산에 미치는 영향)

  • Kong, In-Pyo;Lee, Eun;Cha, Yun-Yeop
    • Journal of Korean Medicine Rehabilitation
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    • v.21 no.1
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    • pp.23-33
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    • 2011
  • Objectives : The present study investigated effects of Artemisia Capillaris Thunberg ethanol extract(EtOH ext). on lowering lipid, anti-oxidation and concentration of plasma inflammatory mediators using rat fed on high oxidized fat. Methods : We divided fat sprague-dawley rats fed on high oxidized into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 4 weeks. We measured concentration of plasma free fatty acid(FFA), plasma triglyceride, plasma total cholesterol, and plasma low density lipoprotein-cholesterol(LDL-cholesterol), plasma high density lipoprotein-cholesterol(HDL-cholesterol), concentration of liver total cholesterol and liver triglyceride (TG), concentration of plasma thiobarbituric acid reactive substance(TBARS) and liver thiobarbituric acid reactive substance(TBARS), glutathione peroxidase (GSH-Px) activity, superoxide dismutase(SOD) activity and catalase(CAT) activity, plasma nitric oxide(NO), ceruloplasmin and ${\alpha}-glycoprotein$. Results : 1. The Artemisia Capillaris Thunberg EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL-cholesterol was increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 2. Concentration of liver total cholesterol and liver TG showed a significantly decrement in all Artemisia Capillaris Thunberg EtOH ext. groups than that of control group. 3. The Artemisia Capillaris Thunberg EtOH ext. groups showed lower values in concentration of plasma TBARS and liver TBARS than that of control group. The values of GSH-Px activity, SOD activity and CAT activity were increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 4. The values of plasma NO, ceruloplasmin and ${\alpha}-glycoprotein$ were decreased in Artemisia Capillaris Thunberg EtOH ext. groups. Conclusions : Based on the results in this study, the Artemisia Capillaris Thunberg EtOH ext. showed a positive effect in lowering lipid, anti-oxidation and decrement of plasma inflammatory mediators.

Anti-inflammatory Effect of Artemisia Capillaris Thunberg in Lipopolysaccharide-exposed Rats (인진호(茵蔯蒿)가 LPS 염증유발 흰쥐의 전염증성 cytokine 생산 및 혈액성상에 미치는 영향)

  • Seo, Yong-Seok;Lee, Eun;Cha, Yun-Yeop
    • Journal of Korean Medicine Rehabilitation
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    • v.20 no.3
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    • pp.27-35
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    • 2010
  • Objectives : The present study investigated anti-inflammatory effect of Artemisia Capilaris Thunberg in lipopolysaccharide-exposed rats. Methods : We divided lipopolysaccharide-exposed Sprague-Dawley rats into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 6 weeks. We measured counts of red blood cell(RBC), the values of hemoglobin(Hb) and packed cell volume(PCV), plasma total protein concentration, albumin concentration, the ratio of albumin/globulin, the activities of plasma glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase(GPT), lactate dehydrogenase(LDH), the counts of white blood cell(WBC), the ratio of neutrophils, lymphocytes, monocytes, basophils, eosinophils, the concentration of plasma interleukin-$1{\beta}$($IL-1{\beta}$), plasma interleukin-6(IL-6), plasma tumor necrosis factor-$\alpha$($TNF-{\alpha}$), plasma interleukin-10(IL-10), the concentration of liver $IL-1{\beta}$ and IL-6, $TNF-{\alpha}$, IL-10. Results : Counts of RBC and the values of Hb and PCV, plasma total protein concentration and albumin concentration, the activities of plasma GOT, GPT and LDH showed no significant difference in the treatment groups. and the ratio of albumin/globulin was increased in Artemisia Capillaris Thunberg groups. The counts of WBC showed lower values in Artemisia Capillaris Thunberg groups than those of control group, In the ratio of neutrophils Thunberg groups. The ration of monocytes, basophils and eosinophils were below 5%, and showed no characteristic trend. The concentration of plasma interleukin-$1{\beta}$($IL-1{\beta}$), plasma inerleukin-6(IL-6) and plasma tumor necrosis factor-$\alpha$($TNF-{\alpha}$) showed a lower values in the Artemisia Capillaris Thunberg groups than those of control group, and the concentration of plasma interleukin-10(IL-10) showed no significant difference in the treatment groups. The concentration of liver $IL-1{\beta}$ and IL-6 showed a lower values in the Artemisia Capillaris Thunberg groups than those of control group, however the concentration of liver $TNF-{\alpha}$ and IL-10 showed no significant difference in the treatment groups. Conclusions : The Artemisia Capillaris Thunberg groups gives positive results of anti-inflammatory response by lipopolysaccharide(LPS) derivation.

Fabrication of Electrospun PVA Nanofibers Loaded with Artemisia capillaris Thunberg Extracts (인진쑥 추출물 함유 PVA 나노섬유 제조)

  • Kim, Myoung Ok;Lee, Jung-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.42 no.2
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    • pp.269-277
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    • 2018
  • This study extracted Artemisia capillaris Thunberg with distilled water and ethanol to investigate its antioxidant effect. We then investigated the possibility of producing nanofibers by an electrospinning process by adding the extracts to polyvinyl alcohol (PVA). The electrospinning method used a PVA concentration of 12wt.%, an applied voltage of 10kV, and a tip-to-collector distance of 15cm. Total polyphenol and total flavonoid contents were measured to verify the antioxidant activity of Artemisia capillaris Thunberg extracts (ACEs). The total polyphenol content of the distilled water extract and the ethanol extract were measured as 218.47 and 271.26mg/g, respectively, and the total flavonoid content of the distilled water extract and the ethanol extract were measured as 141.68 and 34.98mg/g, respectively. As the content of the ACEs in the PVA nanofibers increased, the Diameters of the nanofibers and the uniformity of the diameters decreased. The electrospinning process was fabricated in a relatively uniform form without beads, and the diameters of the nanofibers that were produced ranged from 340 to 390nm. The results of FT-IR, XRD and DSC analyses confirmed that the ACEs were well mixed with the PVA molecules and were electrospun.

Effects of Artemisia Capillaris Thunberg on Apoptosis in HeLa Cells (사철쑥의 HeLa 세포고사 효과)

  • Lee, Hyoung-Ja;Kim, Kee-Hwan;Park, Jong-Kun;Hwang, Eun-Hee
    • Journal of Nutrition and Health
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    • v.41 no.1
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    • pp.22-30
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    • 2008
  • This study analyzes the apoptosis of HeLa cells to see if we can use the Artemisia capillaris Thunberg for the prevention of chronic degenerative diseases. We used the HeLa cells to see what effects the A. capillaris Thunberg had on apoptosis of the cancer cells. We checked the cell activity, cell morphological change, DNA fragmentation, and DNA content after administering 0, 100, 500, 1000, and $2000{\mu}g/ml$ methanol, ethyl acetate, n-butanol extract of the A. capillaris Thunberg. As for the cell viability, the increase of concentration of methanol and ethyl acetate decreased the survival rate of the cell, but the phenomenon was much weakened in n-butanol extract and was not observed in aqueous extract. The higher the density of the methanol, ethyl acetate, n-butanol and aqueous extract was, the lower the survival rate of the HeLa cell was. These extracts obstructed the cell cohesion and caused the blebbing of he cell membrane and fragmentation of the nucleus, both of which are symptoms of apoptosis. Laddering-pattern DNA fragmentation was observed in the groups that were treated with the $1000{\mu}g/ml$ and $2000{\mu}g/ml$ of methanol extract. The DNA content of the cells apoptosis measured by fluorescent-activated cell sorter (FACS) increased as the density of the methanol, ethyl acetate and butanol extract increased. The result of the study shows that A. capillaris Thunberg fosters the apoptosis of HeLa cells, which suggests that the A. capillaris Thunberg has a great potential value as food additives, medicinal supplements for patients with chronic diseases, and preventive measures against cancer.

Screening on Radical Scavenging Activity of Salt Marsh Plants (염생식물로부터 천연항산화 활성소재 탐색)

  • Kim, You-Ah;Lee, Hee-Jung;Seo, Young-Wan
    • 한국생물공학회:학술대회논문집
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    • pp.673-675
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    • 2003
  • The antioxidant activities of methanol and dichloromethane extracts of eighteen salt marsh plants were tested by using 1,1-Diphenyl-2-picryl-hydrazyl(DPPH) at a $100{\mu}g/ml$ concentrations. The methanol extracts of four salt marsh plants(Rosa rugosa Thunberg, Erigeron annuus, Ixeris tamagawaensis kitamura, Artemisia capillaris Thunberg) were found to be most effective in DPPH radical scavenging activity. The DPPH radical scavenging effect of these plants was Artemisia capillaris Thunberg(88.67%), Rosa rugosa Thunberg(87.51%), Erigeron annuus(78.49%), Ixeris tamagawaensis kitamura(69.99%) in order and was comparable with that of natural antioxidant, L-ascorbic acid(96.64%). The next effective ones were the methanol extracts of Teragonia tetragonoides(58.66%) and the dichloromethane extracts of Artemisia capillaris Thunberg(54.67%) and the others did not show a considerable activity. These salt marsh plants were evaluated using the pyrogallol UV-VIS spectrophotometeric method to generate superoxide anion. Among them, the dichloromethane extracts of four salt marsh plants and the methanol extracts of fifteen ones were weak superoxide dismutase-like activities.

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Antimicrobial activity and chemical components of two plants, Artemisia capillaris and Artemisia iwayomogi, used as Korean herbal Injin

  • Seo, Kyoung-Sun;Jeong, Hyung-Jin;Yun, Kyeong-Won
    • Journal of Ecology and Environment
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    • v.33 no.2
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    • pp.141-147
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    • 2010
  • This study compared the antimicrobial activity and chemical composition of two plants used as "Injin" (Korean herbal medicine), namely, Injinho (Artemisia capillaris Thunberg) and Haninjin (Artemisia iwayomogi Kitamura). The ethyl acetate and ether fractions of crude methanol extracts from A. capillaris and A. iwayomogi were tested against three grampositive bacteria (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus), two gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens), and a yeast (Saccharomyceus cerevisiae). The antimicrobial activity of the ethyl acetate and ether fraction of both plants was strong, but that of A. iwayomogi extracts was higher than that of A. capillaris extract for the microbes tested. The minimum inhibitory concentration of the ether and ethyl acetate fraction of A. iwayomogi was highest for P. fluorescens and lowest for S. aureus and E. coli. We analyzed the chemical composition of the ethyl acetate fraction of A. capillaris and A. iwayomogi using gas chromatography-mass spectrometry. The main components of A. capillaris and A. iwayomogi were escoparone (86.82%) and scopoletin (20.47%), respectively.

Effects of Artemisia Capillaris Thunberg on the Plasma and Liver Lipid Metabolism in Rats (사철쑥 (Artemisia Capillaris Thunberg)이 흰쥐 혈액 및 간지질 대사에 미치는 영향)

  • 이형자;황은희
    • Journal of Nutrition and Health
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    • v.35 no.4
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    • pp.421-430
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    • 2002
  • This study was performed to investigate the influence of dietary and extract foods from A. capilliaris Thunberg on plasma and liver lipid metabolism in male Sprague-Dawley rats. For the experiment of liver and plasma lipid metabolism, Rats were find diets containing normal concentrations of fat or high concentrations of lard and two different preparations of A. capillaris Thunberg ; control diet (group C),50 mg/kg body weight A. capillary Thunberg methanol extract (group M), 6 g/kg diet A. capillary Thunberg dried powder (group P), high lard control diet (group L), 50 mg/kg body weight A. capillaris Thunberg with high lard (group LM) and 6 g/kg diet A. capillary Thunberg with hi\ulcorner lard (group LP). Effects of A. capillary Thunberg on plasma total cholesterol. High-density lipoprotein (HDL) cholesterol, Atherogenic index, triglyceride, plasma and liver peroxide contents, fatty acid composition of liver lipid and the distribution of fat droplets of liver. Supplementation of A. capillaris Thunberg resulted in lower plasma cholesterol, atherogenic index and triglyceride, and higher HDL-cholesterol in rats find high lard diets. However, these effects were not observed with low level of fat (groups C, M and P). A shift caused by feeding high lard diets in increased plasma and liver peroxides, saturated fatty arid composition of liver lipid and the more frequent distribution of fat droplets in liver could be reversed by feeding A. capillary Thunberg. This study suggests that A. capillary Thunberg co alter lipid metabolism in plasma and liver.

The analysis of Nutrients in Artemisia capillaris Thunberg (사철쑥 (Artemisia capillaris Thunberg)의 영양성분 분석)

  • Lee, Hyoung-Ja;Hwang, Eun-Hee;Yu, Hyeen-Hee;Song, In-Sang;Kim, Chang-Min;Kim, Myung-Chul;Hong, Jin-Hwan;Kim, Dong-Sul;Han, Sang-Bae;Kang, Kil-Jin;Lee, Eun-Ju;Chung, Hyung-Wook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.361-366
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    • 2002
  • A. capillaris Thunberg is often used as a medicinal herb. This analysis on A. capillaris Thunberg, showing its natural ingredients and nutritive elements, is to provide a better understanding of its content and help find more various ways of use. The ingredients of A. capillaris Thunberg are as follows : 14.12% of crude protein, 4.80% of crude lipid, 2.30% of crude ash, 8.10% of crude fiber, and the rest of the ingredients are vitamins and minerals. Minerals are 3295.02 mg% of K, 2787.01 mg% of P, 1436.01 mg% of Ca, 172.32 mg% of Mg, 21.23 mg% of Fe, 18.02 mg% of Mn, 8.11 mg% of Na, 1.24 mg% of Cu, and 0.002 mg% of Sn, and vitamins are 18602.00 ug% of $\beta$-carotene and 5.82 mg% of ascorbic acid. Fatty acids in A. capillaris Thunberg are of 23.86% of oleic acids (C18:1), 46.67% of saturated fatty acids, 33.40% of monousaturated fatty acids, and 19.83% of polyunsaturated fatty acids. Oleic acid (C18:1) is the most abundant fatty acid in A. capillaris Thunberg. P/S is 0.24. A. capillaris Thunberg contains about 20 kinds of amino acid. The total amount of amino acids is 1345.29 mg%, which can be divided into 79.95% of amino acids and 13.11% of essential amino acids. This 79.95% of amino acids consist of proline, tyrosine, asparagines, glutamic acid, and valine with amount of 438.58mg%, 310.20mg%, 120.30mg%, 118.66mg%, and 88.02mg% respectively. The essential amino acid is 176.83mg%. It is shown that A. capillaris Thunberg contatins various nutrients such as minerals, vitamins, fatty acids, and amino acids, so A. capillaris Thunberg can be regarded as a highly nutritious food.

Comparison of Anti-inflammatory effects between Artemisia capillaris and Artemisia iwayomogi by extraction solvents (인진호(茵蔯蒿)와 한인진(韓茵蔯)의 추출용매별 항염증 효능 비교)

  • Noh, Dongjin;Choi, Jin Gyu;Hong, Soon-Sun;Oh, Myung Sook
    • The Korea Journal of Herbology
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    • v.33 no.3
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    • pp.55-61
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    • 2018
  • Objectives : Artemisia capillaris Thunberg (AC) and Artemisia iwayomogi Kitamura (AI) have been used without distinguishment since ancient times due to similar appearance. In this study, we compared the inhibitory effects of AC and AI on the expression of inflammatory cytokines induced by lipopolysaccharide (LPS) in murine macrophages. Methods : AC and AI were extracted by reflux with distilled water (DW) and 70% ethanol (EtOH). We investigated the inhibitory effects of AC and AI on the expression of nitric oxide (NO), inducible NO synthase (iNOS) and tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$) induced by LPS in macrophages. Results : Firstly, yield of the samples was higher in order of Artemisia iwayomogi DW Extract (AID), Artemisia iwayomogi 70% EtOH Extract (AIE), Artemisia capillaris DW Extract (ACD) and Artemisia capillaris 70% EtOH Extract (ACE). All of the samples were not toxic in macrophages. The inhibitory effect of the samples on LPS-induced NO expression was stronger in the order of AIE, ACE, AID and ACD. The inhibitory effect of the samples on LPS-induced inducible iNOS expression was stronger in the order of AIE, ACE and AID. Effect of ACD was same with that of AID. In addition, inhibitory effect of the samples on LPS induced $TNF-{\alpha}$expression wes stronger in the order of AIE, ACE, AID and ACD. Conclusion: These results showed that AI would be more effective than AC and 70% EtOH would be more effective than DW as an extraction solvent in inflammatory diseases.