• Title, Summary, Keyword: Aspergillus fumigatus

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Regulation of Development in Aspergillus nidulans and Aspergillus fumigatus

  • Yu, Jae-Hyuk
    • Mycobiology
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    • v.38 no.4
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    • pp.229-237
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    • 2010
  • Members of the genus Aspergillus are the most common fungi and all reproduce asexually by forming long chains of conidiospores (or conidia). The impact of various Aspergillus species on humans ranges from beneficial to harmful. For example, several species including Aspergillus oryzae and Aspergillus niger are used in industry for enzyme production and food processing. In contrast, Aspergillus flavus produce the most potent naturally present carcinogen aflatoxins, which contaminate various plant- and animal-based foods. Importantly, the opportunistic human pathogen Aspergillus fumigatus has become the most prevalent airborne fungal pathogen in developed countries, causing invasive aspergillosis in immunocompromised patients with a high mortality rate. A. fumigatus produces a massive number of small hydrophobic conidia as the primarymeans of dispersal, survival, genome-protection, and infecting hosts. Large-scale genome-wide expression studies can now be conducted due to completion of A. fumigatus genome sequencing. However, genomics becomes more powerful and informative when combined with genetics. We have been investigating the mechanisms underlying the regulation of asexual development (conidiation) and gliotoxin biosynthesis in A. fumigatus, primarily focusing on a characterization of key developmental regulators identified in the model fungus Aspergillus nidulans. In this review, I will summarize our current understanding of how conidiation in two aspergilli is regulated.

Studies on the Maildew-Proofing of Cotton Fabrics by Growth of Aspergillus niger H-18 and Aspergillus fumigatus E-29 (면섬유에 번식하는 Aspergillus niger H-18과 Aspergillus fumigatus E-29에 대한 방미제의 항균효과)

  • 홍정민
    • Journal of the Korean Home Economics Association
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    • v.29 no.2
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    • pp.47-53
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    • 1991
  • A. niger H-18 and A. fumigatus E-29 wrer selected for their strong abilities to produce cellulase. The λd numerical values of the cotton fabrics inoculated with A. niger H-18 and A. fumigatus E-29 were 580 nm for the both strains of molds. By the growth of molds, lightness, original color scale, and grey scale of the fabrics gradually decreased while chroma increased. The minimum inhibitory concentrations of mold-proofing agents, such as Leperon WL, 8-hydroxyquinoline copper acetate, trimethylol melamine and dimethyl ethylene urea was 50 ppm. Glycoxale was not effective at the above mentioned concentration. Since Leperon WL, trimethylol melamine and dimethyl ethylene urea effectively inhibited the growth of A. niger H-18 and A. fumigatus E-29, tensile strength and elongation of the fabrics were not changed. however, cotton fabrics treated with glycoxale of the fabrics were not changed. However, cotton fabrics treated with glycoxale and inoculated with A. niger H-18 and A. fumigatus E-29 showed decreased in tensile strength by 31.1% and 33.9%, respectively.

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Aspergillus Fumigatus Infection in a Captive Carribean Flamingo (Phoenicopterus Ruber) (국내 사육중인 쿠바홍학에서 Aspergillus fumigatus 감염증례)

  • Kim, Bo-Sook;Jung, Yong-Mok;Kim, Han-Jun;Ko, Ji-Seung;Do, Sun-Hee
    • Journal of Veterinary Clinics
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    • v.30 no.1
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    • pp.71-74
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    • 2013
  • Aspergillus fumigatus (A. fumigatus) is one of the most important mycotic infection in wild animals stressed by captivity and concurrent disease. A 14-year-old Carribean flamingo controlled bumble foot with antibiotics died after having dyspnea, depression, and anorexia. Necropsy findings revealed pyogranulomatous inflammations of lung and greenish gray-colored mold on air sacs. A carribean flamingo was diagnosed as pyogranulomatous pneumoniae due to A.fumigatus infection by results of lung tissue culture and microscopic examination.

Antifungal activities of peptides with the sequence 10-17 of magainin 2 at the N-termini against aspergillus fumigatus (Antifungal Activities of Peptides with the Sequence 10-17 of Magainin 2 at the N-termini against Aspergillus fumigatus)

  • Lee, Myung Kyu;Lee, Dong Gun;Shin Song Yub;Lee, Sung Gu;Kang Joo Hyun;Hahm, Kyung Soo
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.274-278
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    • 1996
  • Two peptides, MA-inv AND MA-ME, with the sequence 10-17 of maganin 2 at their-N-termini were designed and synthesized. The peptides had higher antifungal activities against Aspergilus fumigatus without hemolytic activities. The minimal inhibition concentratory (MIC) values of both peptides against A. fumigatus were 5 .mu.g/ml, whereas those of the native peptides, magainin 2 and melittin, were 10.mu.g/ml. At 3 .mu.g/ml, MA-inv and MA-ME inhibited the mycelium growth of A. fumigatus by 94.6% and 97.3% respectively, whereas magainin 2 and melittin inhibited by 62.2% and 32.4, respectively. MA-inv showed up to 80% inhibition of (1, 3)-.betha.-D-glucan synthase activity of A. fumigatus. The peptides also showed up to 80% inhibition of (1, 3)-.betha.-D glucan synthase activity of A. fumigatus. The peptides also showed antifungal activities for other fungi of Aspergillus sp. However, the antibiotic activities of MA-ME against Escherichia coli, Bacillus subtilis and Fusarium oxysporum were more effective than those of MA-inv, suggesting that the C-terminal sequences of MA-inv and MA-ME may also influence their antibiotic activities. These results suggest that the N-terminal sequence of the designed peptides, KKFGKAFV, is important for their antifungal activities against A. fumigatus and their C- terminal sequences are related to the organism selectivity.

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Aspergillus Fumigatus Infection in Wild Goose (흑기러기에 있어서 Aspergillus fumigatus에 의한 감염증)

  • 신태균;양기천;이헌준;김옥녀;김우택;권오덕;이두식
    • Journal of Veterinary Clinics
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    • v.13 no.2
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    • pp.195-197
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    • 1996
  • 제주도에 서식중인 흑기러기(Branta bernicla)가 식욕감퇴, 점진적인 수척, 호흡촉박 등을 나타내다 폐사하였다. 부검소견으로서는 폐 및 복부기낭에 다수의 위약한 흰색-황색 결절이, 병리조직학적검사결과 괴사병소에 균사와 Aspergillus의소포가 확인되었다. 배양결과 Aspergillus fumigatus 감염증으로 진단된 국내 최초의 보고로 생각된다.

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Chronic Aspergillus fumigatus Infection in a Ostrich (Struthio camelus) (타조에서 Aspergillus fumigatus 만성 감염증)

  • Kiku Matsuda;Seol, Min-Suk;Kim, Yeo-Jung;Valieryevna, Yetobayeva-Irina;Lee, Hyoung-Ja;Lim, Chae-Woong
    • Journal of Veterinary Clinics
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    • v.19 no.1
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    • pp.107-109
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    • 2002
  • An 8-month-old ostrich (Struthio camelus) which had shown lethargy died in 2-day clinical course. Gross necropsy revealed greenish gray mold grow on the wall of thickened air sacs and multiple tiny nodules on the liver. Microscopically, the granulomatous lesions of air sac membrane, lung, and liver contained numerous septate, branching fungal hyphae. The typical conidial heads of fungi were observed in inner membrane of inflammatry thickened air sacs. This case was a chronic and systemic mycotic air sacculitis and pneumonitis caused by Aspergillus fumigatus in a ostrich at a zoo.

Study of Aspergillus Species from Clinical Specimen Isolate (임상검체에서 분리된 Aspergillus Species의 연구)

  • Lee, Jang Ho;Koo, Bon-Kyung
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.1
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    • pp.15-21
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    • 2016
  • Aspergillus is the most common opportunistic fungus causing infection. Aspergillus is the most morphologically identified in the laboratory. Recently, molecular genetic methods have been proposed for identification of fungi that unidentified morphologically or identified genus level. Of 475 cases of Aspergillus isolated from clinical specimens, there were Aspergillus fumigatus 257 (54.1%), A. niger 101 (21.3%), A. flavus 43 (9.1%), A. terreus 29 (6.1%), Aspergillus nidulans 2 (0.4%), Aspergillus clavatus 1 (0.2%), and the Aspergillus species 42 (8.8%). Eleven cases of unidentified or identified at the genus level included Aspergillus fumigatus 5, Aspergillus falvus 1, Aspergillus terreus 1, and Aspergillus lentulus 1 was identified in the sequencing of the strain level. It was identified as Aspergillus versicolor 2, and Emericella parvathecia 1. 92.2% of Aspergillus was identified as a possible morphological, 8.8% could not be identified at the species level. Sequence-based molecular analysis using the ITS and D1D2 is considered useful for identification of the species level.

Screening of Microorganisms with High Poly (butylene succinate-co-butylene adipate)-Degrading Activity (고활성 Poly(butylene succinate-co-butylene adipate) 분해균의 선발)

  • Kim, Mal-Nam;Lee, Sun-Hee;Kim, Wan-Gyu;Weon, Hang-Yeon
    • Korean Journal of Environmental Biology
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    • v.25 no.3
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    • pp.267-272
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    • 2007
  • Microorganisms capable of degrading poly(butylene succinate-co-butylene adipate) (PBSA) were isolated from 40 soil samples such as landfill site soil, cultivating soil and activated sludge soil from 20 different sites in Korea by using the enrichment culture and the clear zone test at $37^{\circ}C$. Based on the 16S rDNA sequences, the isolated bacterium was identified to be Streptomyces sp. PBSA-1. Morphological and cultural characteristics were employed for the identification of the isolated fungi and they were proved to be Aspergillus fumigatus PBSA-2 and Aspergillus fumigatus PBSA-3. The PBSA degradation activity of the isolated microorganisms was enhanced through the serial acclimation in PBSA plate medium. The PBSA degrading microorganisms appeared to be highly active for the PBSA degradation in that 83% of PBSA was degraded by Streptomyces sp. PBSA-l, and 65% and 75% of PBSA was mineralized by A. fumigatus PBSA2 and A. fumigatus PBSA-3 respectively during 40 days of the modified Sturm test.

Studies on the Conditions of Enzyme Production of Endocellular Cytosine Deaminase from Aspergillus fumigatus IFO 5840 (Aspergillus fumigatus IFO 5840의 균체내 Cytosine Deaminase의 생성에 관한 연구)

  • 김재근;하영득
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.2
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    • pp.179-186
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    • 1991
  • The nutritional requirement and cultural condition such as carbon and nitrogen sources, cultural temperature, initial pH, cultural time and aeration for the production of endocellular cytosine deaminase from Aspergillus fumigatus IFO 5840 were investigated. The cultural broth giving maximum cytosine deaminase yield was found to consist of 2% glucose as a carbon source and 1% yeast extract and 0.1% peptone as a nitrogen source. Optimal initial pH of the culture broth was pH 8.5 and the enzyme production in the cell usually reached a maximum after 28 hours of cultivation in the 500ml shaking flask containing 100ml broth at $30^{\circ}C$. The endoenzyme production of the used strain was inhibited by inorganic nitrogen, but activated by orgainc nitrogen, yeast extract.

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The Chemical Components and Nutritional Evaluation of Aspergillus fumigatus Cells (Aspergillus fumigatus균체의 화학적 성분과 영양학적 평가)

  • 최종덕;조성환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.1
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    • pp.41-47
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    • 1995
  • This experiments was designed to evaluated the chemical components and nutrition of Aspergillus fumigatus cells. This dried fungal mycellia was consist of crude protein 48.5%, crude lipid 2.9%, carbohydrate 44.7% and total ash 3.4%, respectively. The major fatty acid of total lipid were 27.9% of linoleic acid, 24.6% of oleic acid, 15.4% of palmitic acid and 10.6% of linolenic aicd. Amino acid analysis indicated that the protein was rich in aspartic acid, glutamic acid, leucine, lysine but poor in cystein, methionine, histidine. The fungal cake of Aspergillus fumigatus, when dried and specially processed, has been found to serve as a source of protein in place of soybean meal in the diet of experimental mice. Animal were fed a control diet first, and an incease in weight proved the formulation to be satisfactory. At the end of a 30-day period, the experimental mice showed increases in weight comparable to those of the control animals. The net protein efficiency ratio for the control diet was 3.42$\pm$0.15 and the fungal protein and succinylated fungal protein with DL-methionine they were 3.12$\pm$0.39 and 2.98$\pm$0.06 respectively. This supports the view that dried and succinylated fungal protein can be substituted as a protein source.

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