• Title, Summary, Keyword: Bacillus strain

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Characterization of a Mosquitocidal Delta-endotoxin from Bacillus thuringiensis subsp. guiyangiensis strain 21-2(H serotype 43) (모기 살충성 내독소를 생산하는 Bacillus thuringiensis subsp. guiyangiensis 21-2균주(H serotype 43)의 특성)

  • 김위종;김광현
    • Microbiology and Biotechnology Letters
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    • v.27 no.5
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    • pp.359-363
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    • 1999
  • To prevent appearance of resistant mosquitoes against $\delta$-endotoxin of bacillus thuringiensis subsp. israelensis (Bti) in field, a mosquitocidal Bacillus thuringiensis strain 21-2(Bt21-2) producing a new type of $\delta$-endotoxin was isolated. The strain Bt 21-2 belongs to H serotype 43, B. thuringiensis subsp. guiyangiensis (Btg). The $\delta$-endotoxins from the strain Bt 21-2 and the strain Bti were a cuboid shape morphologically, but the $\delta$-endotoxin of the strain Bt 21-2 was composed of 150, 90 and 70kDa proteins on SDS-PAGE, and the antigenicity of $\delta$-endotoxin of the strain Bt 21-2 was different from that of the strain Bti on immunoblot. The $\delta$-endotoxin gene of the strain Bt 21-2 was not amplified with specific primers of $\delta$-endotoxin gene (cry4A and cry4B) of the strain Bti on PCR.

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Isolation and Identification of Bacteria Producing a Soybean Milk Clotting Enzyme (두유 응고효소 생산균의 분리 및 동정)

  • 하덕모;이철우
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.109-114
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    • 1989
  • Seventeen bacterial strains producing an extracellular soybean milk clotting enzyme were Isolated from 150 soil samples, and identified as Bacillus cereus(8 strains), Bacillus pumilus(8 strains) and Bacillus licheniformis (1 strain). Among them, Bacillus pumilus strain 118 and Bacillus licheniformis strain 192 showed relatively high soybean milk clotting activity. The coagulability of enzymes from these strains decreased as the pH of soybean milk was increased from 6.0 to 7.0. The optimum temperature for soybean milk clotting activity was $65^{\circ}C$.

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Isolation of Neutral Protease Hyperproducing Bacillus sp. KN103N and Some Properties of the Enzyme (中性 Protease 高 生産性 Bacillus sp. KN103N의 分離 및 酵素의 特性)

  • Kim, Hong-Rip;O, Pyong-Su
    • Microbiology and Biotechnology Letters
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    • v.19 no.2
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    • pp.116-121
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    • 1991
  • A bacterial strain KN, which highly produced a protease, was isolated from several soil samples and identified to to belong to the genus Bacillus. We selected mutant strain Bacillus sp. KN103N, which was hyperproducer of protease and was resistant to D-cyclowerine, from the strain KN by several steps of mutagenesis. Neutral protease productivity of mutant strain KN103N was about 55 times as much as that of the original strain KN. The optimum pH and temperature for the enzyme activity were 7.0 and 50$^{\circ}C$, respectively and the enzyme was relatively stable at pH6.0~8.0 and below 40$^{\circ}C$. The enzyme was inactivated by EDTA, but not by DFP. These results indicate that the enzyme from Bacillus sp. KN103N was a neutral (metallo-) protease.

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Isolation and Identification of a Bacteriolytic Enzyme-producing Bacterial Strain from Pusan Coastal Sea (해양에서 용균효소를 분비하는 균주의 분리와 동정)

  • 진성현;류병호
    • Microbiology and Biotechnology Letters
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    • v.23 no.5
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    • pp.580-587
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    • 1995
  • In order to produce the bacteriolytic enzyme, bacterial strains capable of excreting a large amount of the enzyme were screened from the coastal sea water samples in Pusan. The bacterial strain SH-1, which showed the highest activity among 43 bacteriolytic enzyme producing bacteria, was finally selected for further studies. The strain SH-1 was an endospore-forming grampositive rod, and the position of spore was paracentral. These morphological characteristics assigned the isolated strain to the morphological group I classified by Gordon. The fatty acid composition of the bacterial stain was analyzed to be consisted of branched chains of iso-Cn and anteiso-Cn. Based on the percent content of the branched chain (93.85%), the isolates could be identified as a species of Bacillus. According to the experimental results of the API system (API 50CHB & API 20E) the strain was identified as Bacillus subtilis. Numerical texonomy, in which 82 major characters were examined using several species of Bacillus as the standard bacteria, indicated that the strain SH-1 showed 90% similarity to Bacillus subtilis. Thus, the isolated strain SH-1 could be identified as Bacillus subtilis.

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Screening and Identification of the Fibrinolytic Bacterial Strain from Jeot-Gal, Salt-fermented Fish (젓갈류로부터 혈전용해 균주의 분리 및 동정)

  • Jang, Young-Ryeol;Kim, Won-Keuk;Kwon, Ik-Boo;Lee, Hyun-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.655-659
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    • 1998
  • Bacterial strain showing the strong fibrinolytic activity (2.04 plasmin unit) was screened from Jeot-Gal, Korean salt-fermented fish collected from various region. For the identification, when the strain was characterized morphologically, culturally, and biochemically, it was identified to Bacillus pumilus. And, when the fatty acids composition of the strain was analyzed, it was identified to Bacillus atropheus. Finally, the 16S rRNA partial sequence (V3 region) showed that the fibrinolytic stain screened from Jeot-Gal was identified as Bacillus subtilis. So, we named it Bacillus subtilis KJ-48.

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Isolation and Characterization of Thermophilic Bacillus sp. UJ03 from Spent Mushroom (Flammulina velvtipes) Substrates (팽이버섯 수확 후 배지로부터 고온성 Bacillus sp. UJ03의 분리 및 특성)

  • Gal, Sang-Wan;Cho, Soo-Jeong
    • Journal of Life Science
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    • v.21 no.10
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    • pp.1481-1486
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    • 2011
  • Spent mushroom substrate (SMS) is a by-product remained after a crop of mushrooms. About seven thermophilic strains were isolated from SMS (Flammulina velvtipes). Among them, one isolate, designated UJ03, showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium, potentially. The strain UJ03 produced cellulase and xylanase as extracellular hydrolases. The strain UJ03 was identified as a member of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain UJ03 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus amyloliquefaciens with sequence similarity of 98.9%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain UJ03 was classified within the genus Bacillus, for which the name Bacillus sp. UJ03 is proposed. The antifungal compound from Bacillus sp. UJ03 was similar to lipopeptide iturin A of Bacillus sp.

Dominant-species Variation of Soil Microbes by Temperate Change (온도변화에 기인한 토양미생물 우점종의 변화에 관한 연구)

  • Park, Kap-Joo;Lee, Byeong-Chol;Lee, Jae-Seok;Park, Chan-Sun;Cho, Myung-Hwan
    • Korean Journal of Environmental Biology
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    • v.29 no.1
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    • pp.52-60
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    • 2011
  • Today, the weather is changing continually, due to the progress of global warming. As the weather changes, the habitats of different organisms will change as well. It cannot be predicted whether or not the weather will change with each passing day. In particular, the biological distribution of the areas climate change affects constitutes a major factor in determining the natural state of indigenous plants; additionally, plants are constantly exposed to rhizospheric microorganisms, which are bound to be sensitive to these changes. Interest has grown in the relationship between plants and rhizopheric microorganisms. As a result of this interest we elected to research and experiment further. We researched the dominant changes that occur between plants and rhizospheric organisms due to global warming. First, we used temperature as a variable. We employed four different temperatures and four different sites: room temperature ($27^{\circ}C$), $+2^{\circ}C$, $+4^{\circ}C$, and $+6^{\circ}C$. The four different sites we used were populated by the following species: Pinus deniflora, Pinus koraiensis, Quercus acutissima, and Alnus japonica. We counted colonies of these plants and divided them. Then, using 16S rRNA analysis we identified the microorganisms. In conclusion, we identified the following genera, which were as follows: 10 species of Bacillus, 2 Enterobacter species, 4 Pseudomonas species, 1 Arthrobacter species, 1 Chryseobacterium species, and 1 Rhodococcus species. Among these genera, the dominant species in Pinus deniflora was discovered in the same genus, but a different species dominated at $33^{\circ}C$. Additionally, that of Pinus koraiensis changed in both genus and species which changed into the Chryseobacrterium genus from the Bacilus genus at $33^{\circ}C$.

Characterization of Mosquitocidal Bacillus thuringiensis Strain H9B (모기유층에 대한 살충성 Bacillus thuringiensis H9B 균주의 특성)

  • 이기희;김광현;김병우
    • Microbiology and Biotechnology Letters
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    • v.21 no.5
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    • pp.393-398
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    • 1993
  • One strain of mosquitocidal Bacillus thuringiensis, H9B, was isolated from soil. The biochemical characteristics and flagella antigenicity of the strain H9B is similar to that of B. thuringiensis subsp. darmstadiensis. The delta-endotoxin of the strain H9B coincided with that of B. thuringiensis subsp. darmstadiensis strain 73E10-2 on agarose double immunodiffusion test. The delta-endotoxin of B. thuringiensis subsp. israelensis contains hemolysin fragment (28 kb) on SDS-PAGE when the delta-endotoxin was solubilized in alkali, while that of the strain H9B does not contain 28 kb protein.

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The studies on microbe isolated from the cocoon in Korea. (Isolation and identification of bacteria) (한국산 잠견에서 분리된 미생물에 관한 연구 제 1보 잠견에서 분리된 Bacteria의 분리동정)

  • 이상원;이철준
    • Journal of Sericultural and Entomological Science
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    • v.7
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    • pp.53-63
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    • 1967
  • In order to identify the bacteria living on the cocoons in Korea, the isolated bacterias' morphological. cultural and physiological characters has been determined through the detailed study. The second aim of this experiment was to protect against the bacteria which damage silk protein during storage. 1. The twelve strains of the bacteria were isolated and identified in the cocoons produced in Korea. The results of the identification are as the following. No 1, No 8; Bacillus subtilis variation No 2, ; Bacillus stearothermophilus No 3, ; Bacillus circulans No 5, No 6; Bacillus thuringiensis No 7, No 11; Bacillus brevis No 12, No l0; Bacillus cereus variation

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Isolation of Bacillus atrophaeus MPL-01 from A Wild Boar and Characterization of Its Antifungal Activity (멧돼지 대장으로부터 Bacillus atrophaeus MPL-01의 분리 및 항진균 활성의 특성)

  • Yun, Sung-Jo;Rho, Jae-Young
    • Korean Journal of Microbiology
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    • v.49 no.2
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    • pp.195-199
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    • 2013
  • A bacterial strain MPL-01 was isolated from the large intestine of a wild boar. The strain was shown to have morphological, physiological and biochemical characteristics, fatty acids composition typical of Bacillus. The 16S rRNA gene sequence showed that the isolate formed distinct phyletic line that was most closely related to this of Bacillus atrophaeus (99.99%). It was proposed that the strain is classified as B. atrophaeus MPL-01. The strain MPL-01 exhibited the strongest antifungal activity against Colletotrichum acutatum, the pathogen of anthracnose of chili peppers. The ethyl acetate extract of culture filtrate possessed not only the antifungal activity but also the bio-surfactant activity. Therefore, the strain MPL-01 could be a useful bacterium in the development of bio-control process against the pathogenic fungi.