• Title, Summary, Keyword: Bifidobacterium

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Improved Selective Medium for Isolation and Enumeration of Bifidobacterium sp. (개량된 Bifidobacterium의 선택배지 개발)

  • Ji, Geun-Eog;Lee, Se-Kyung;Kim, In-Hee
    • Korean Journal of Food Science and Technology
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    • v.26 no.5
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    • pp.526-531
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    • 1994
  • In order to develop a new improved selective medium for the Bifidobacterium sp. from the human fecal samples, one hundred eight Bifidobacterium strains were isolated and identified. Sensitivity test for the antibiotics and antimetabolites and test for the specific substrate were performed to obtain basic data for the development of the Bifidobacterium selective medium. TOS(transgalactosylated oligosaccharide) was shown to be preferentially used by Bifidobacterium sp.. Sodium propionate promoted the growth of Bifidobacterium while inhibiting other intestinal bacteria. Upon these results, TP medium was designed and shown to be very effective for the selection of Bifidobacterium and better than Mitsuoka BS medium.

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Fermentation of Carrot Juice by Bifidobacterium (Bifidobacterium에 의한 당근발효)

  • Park, So-Young;Ko, Young-Tae;Lee, Joo-Yeon;Mok, Chul-Kyoon;Park, Jong-Hyun;Ji, Geun-Eog
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.571-575
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    • 1997
  • In the present study, characterization of fermented carrot juice by Bifidobacterium was performed. When inoculated at the level of $10^6\;CFU/mL$ with various Bifidobacterium strains, cell growth of B. longum, B. adolescentis and B. infantis reached more than $10^8\;CFU/mL$. On the other hand, B. bifidum strains reached less than $10^8\;CFU/mL$. Compared with carrot, grape juice did not allow the growth of Bifidobacterium, while peach juice and orange juice were as good as carrot for the growth of Bifidobacterium. On mixed culture with Lactobacillus, growth of Bifidobacterium decreased and cell death rate increased considerably. On panel test, Bifidobacterium cultured-carrot juice showed high score on sensory test than non-fermented carrot. Therefore, fermentation may lead to the quality improvement of carrot juice by combining health-promoting effect of Bifidobacterium and high nutrition value of carrot.

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Characterization of Functional Kimchi Using Bifidobacterium lactis (Bifidobacterium lactis를 이용한 기능성 김치의 특성)

  • Kim, Tae-Woon;Park, Ae-Kyung;Kim, Gum-Ran;Lee, Jung-Min;Chung, Dae-Kyun;Kim, Hae-Yeong
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.924-927
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    • 2003
  • This study was conducted to investigate the application of bifidobacteria on kimchi. Among several Bifidobacterium species, we selected Bifidobacterium lactis (DSM 10140), which is resistant to oxygen, acid and salt. Bifidobacterium lactis was cultured in a supplemented deMan, Rogosa and Sharpe (SMRS) medium under aerobic conditions. Its acid-tolerance and salt-tolerance were pH 3.0 and 3.5% (NaCl), respectively. The viability of Bifidobacterium lactis added to kimchi was confirmed by PCR, using specific primers on Bifidobacterium lactis. In sensory evaluation, kimchi containing Bifidobacterium lactis showed similar scores in overall acceptability with the control kimchi. Consequently, these results showed that it would be possible to prepare functional kimchi using Bifidobacterium.

Anti-Helicobacter pylori Activity of Bifidobacterium spp.

  • Bae, Eun-Ah;Kim, Dong-Hyun;Han, Myung-Joo
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.532-534
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    • 2000
  • The inhibitory effects of different Bifidobacterium spp. on the growth of Helicobacter pylori (HP) were investigated. A significant suppression of HP growth occurred only when HP was inoculated onto a petri dish containing 0.1 mg/ml of Bifidobacterium spp. When HP was separately cultured with B. breve K-110, B. catenulatum K-309, B magnum K-311, B. magnum K-321, and B. cuniculi K-513, the urease activity was also inhibited by these Bifidobacterium spp. Therefore, it appears that these Bifidobacterium spp. excrete a heat-labile inhibitory component for HP growth into the culture medium. Although most organic acids produced by the Bifidobacterium spp. inhibited the growth of HP, the HP growth was not inhibited by the physiological concentrations of organic acids produced in bifidobacteria-cultured media. Accordingly, these results suggest that some Bifidobacterium spp. may produce antibiotic-like compounds (bacteriocins).

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Isolation and Identification of Superior Bifidobacterium strains from Korean Feces

  • Kim, Ji-Yeon;Lee, Yun-Jong;Choe, Su-Im;Heo, Tae-Ryeon
    • 한국생물공학회:학술대회논문집
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    • pp.422-425
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    • 2001
  • For isolating and identifying Bifidobacterium spp. originating from Korea, feces were sampled from healthy Korean infants nursery school and postpartum care center. Through the use of gram staining and microscopic examination for cell morphology, 87 bacterial strains presumed to be the Bifidobacterium strains were isolated from 59 Koreans. To identify the Bifidobacterium strains at the genus level, these bacteria were then analyzed using the TLC method. As a result, 29 of the isolated strains were confirmed as members of the genus Bifidobacterium. 29 Bifidobacterium strains were tested acid, bile salts and oxygen tolerance and investigated antioxidative effect specially. And determined the superiority of 5 strains out of 29 Bifidobacterium strains. Finally, the selected bifidobacterium was identified with using designed 16S-ITS rDNA primer.

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Bifidogenic Effect of Glucooligosaccharide Prepared from Glucose by Extrusion Process

  • Ahn, Jun-Bae;Hwang, Jae-Kwan;Kim, Chong-Tai;Lee, Ke-Ho;Park, Jong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.7 no.3
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    • pp.174-179
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    • 1997
  • In order to investigate effect of glucooligosaccharide (GOS) prepared by extrusion process as a bifidogenic factor, cultivation of Bifidobacterium sp., Bacteroides fragilis and Clostridium perfringens was done and analyzed. B. fragilis and C. perfringens were able to utilize only 16% and 11% of the oligosaccharides in GOS, respectively, whilst Bifidobacterium sp. FBD-22 could utilize 38%. Especially, many kinds of oligo saccharides in GOS were able to be utilized selectively only by Bifidobacterium sp.. In case that GOS, as a carbon source, was used in the co-cultivation by Bifidobacterium sp., B. fragilis and C. perfringens, growth of Bifidobacterium sp. was not influenced by the existence of B. fragilis and C. perfringens. Bifidobacterium sp. showed advantage on carbon source competition for GOS with B. fragilis. Acetic acid, antimicrobial agent in the intestine, was produced two times more from GOS than glucose in co-cultures of three strains. Therefore, it is suggested that GOS can be a potent bifidogenic factor which proliferates the population of Bifidobacterium sp. and may finally improve the intestinal environments of human.

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Isolation and Identification of the Antilisterial Bifidobacterium Isolates from the Infants Fecal Samples (유아의 분변으로부터 항리스테리아 활성의 Bifidobacterium 속 균주의 분리 및 동정)

  • Kim, Song-Yi;Kim, Ki-Hwan;Youn, Soon-Yong;Yoon, Sung-Sik
    • Journal of Dairy Science and Biotechnology
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    • v.24 no.1
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    • pp.19-28
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    • 2006
  • This study was conducted to isolate antilisterial strains of the Bifidobacterium isolates from the infant feces. The bifidobacteria were isolated anaerobically on BL agar and screened for their inhibitory activity on the MRS-cysteine medium against three foodborne pathogens: Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus. Among the 52 bifidobacterial isolates, 5 strains(A24, Bl, B6, B10, and Bl2) were finally selected based on their stronger antilisterial activity against Listeria monocytogenes than other isolates tested. Morphologically, all the isolates were typically shown Y-and V-shaped under electron microscopic examination. Each isolate was primarily subjected to identification by a polymerase chain reaction(PCR) using a genus-specific primer designed for targeting the 16S rRNA gene sequence, and confirmed the primary identification data using an API-kit(Biomeriuex, France), commercially available product for identification based on biochemical and physiological traits. Of the isolates with antilisterial activity, strain A24 was finally confirmed as the Bifidobacterium longum A24.

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High Density Cell Culture of Bifidobacterium by Optimization of Medium Composition and Culture Conditions. (배지조성 및 배양환경 최적화에 의한 Bifidobacterium의 고농도 배양)

  • 송수한;김택범;지근억;오훈일;오덕근
    • Microbiology and Biotechnology Letters
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    • v.30 no.1
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    • pp.63-67
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    • 2002
  • Bifidobacterium strain was isolated from the feces of brast fed infants. The isolated strain was identified as Bifidobacterium longum by 16S rRNA sequence analysis and named as Bifidobacterium SH2. The MRS medium was modified to obtain high density cells of Bifidobacterium SH2. The optimal medium was determined to be 50 g/L lactose, 10 g/L beef extract, 10 g/L peptone, 5 g/L yeast extract, 7 g/L sodium acetate, 2 g/L ammonium citrate, 2 g/L disodium phosphate,1 g/L tween 80, 0.2 g/L MnSO$_4$ and 0.5 g/L L-cysteine. The pH and temperature were optimized as 5.0 and $37^{\circ}C$, respectively. Through out the optimization of medium composition and culture conditions, the dry cell weight and viable cell count were 2.5 times and 1.8 times higer than those in MRS medium, respectively.

Effect of Viability and Integrity of Bifidobacterium on Suppression of Allergy in Mice

  • Kim Hye-Young;Geun Eog-Ji
    • Journal of Microbiology and Biotechnology
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    • v.16 no.7
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    • pp.1010-1016
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    • 2006
  • The effects of the cell viability and integrity of Bifidobacterium on suppression of allergy were investigated. C3H/HeJ mice were sensitized on weeks 3, 4, 6, and 8 with ovalbumin and choleratoxin to induce an allergic reaction. Mice fed 0.2% of live, disrupted, or heat-killed Bifidobacterium bifidum BGN4 in the pellets of their diet for 8 weeks starting 2 weeks before initial sensitization differentially suppressed the allergy response in terms of levels of IgE and IgG1 in their sera, and symptoms on their tails. Viable Bifidobacterium was more effective than disrupted or heat-killed cells in suppressing the allergy. Growth inhibition, which occurred in the sham group at week 4, did not occur in the treated groups. These results show that Bifidobacterium has a suppressive effect on the allergic response of mice, and that the viability and integrity of the Bifidobacterium is required for effective suppression in our experimental model.

Isolation of Macrophage-activating Bifidobacterium for the Manufacture of Fermented Rice Products (쌀 발효제품 제조를 위한 마크로파지활성 비피더스균의 선발)

  • 차성관;홍석산;지근억;목철균;박종현
    • Microbiology and Biotechnology Letters
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    • v.27 no.6
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    • pp.509-514
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    • 1999
  • Forty seven amylolytic Bifidobacterium strains were isolated on starch-containing agar medium from the faecal samples of the various age groups of Korean. From these amyloytic Bifidobacterium spp., two strains of KFRI 1535, identified temporarily as Bifidobacterium longum, and KFRI 1550, identified as Bifidobacterium breve, showed great macrophage-stimulating activity for the production of tumor necrosis factor-$\alpha$ and inteleukin-6. As the cell concentration increased the cytokine production increased, although in some strains the cytokine levels started to decline over cell concentration increased the cytokine production increased, although in some strains the cytokine levels started to decline over cell concentration of $250\mu\textrm{g}$/ml. the strains which showed high cytokine-stimulating activity generally showed greater production of nitric oxide even though differences were less between strains. Selected Bifidobacterium strains were compared for their fermentation capability in saccharified rice solution and in apple pomace mixture.

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