• Title, Summary, Keyword: Bifidobacterium spp

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Effects of Bifidobacterium spp. isolated from the feces of healthy adults on the enhancement of the presentation of exogenous particulate antigen in association of MHC Class I

  • Han, Shin-Ha;Yun, Yun-Ha;Song, Young-Cheon;Park, Eun-Jung;Choi, Sung-Sook;Ha, Nam-Joo;Kim, Kyung-Jae
    • Proceedings of the PSK Conference
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    • pp.205.1-205
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    • 2003
  • Bifidobacterium spp. is nonpathogenic. Gram-positive and anaerobic bacteria, which inhabit the intestinal tract of humans and animals. Bifidobacterium spp. plays important roles in human health. However. the influence of exogenous factors on species composition of fecal bifidobacteria is still unclear. In this study, we wished to determine whether presentation of exogenous OVA (10 $\mu\textrm{g}$/$m\ell$) could be enhanced by the culture supernatant of ten Bifidobacterium spp. (omitted)

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In vitro selection of lactic acid bacteria for probiotic use in pig (양돈용 생균제 균주개발을 위한 유산균주 선발)

  • Ryu, Ji-Sook;Han, Sun-Kyung;Shin, Myeong-Soo;Lee, Wan-Kyu
    • Korean Journal of Veterinary Service
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    • v.32 no.1
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    • pp.33-41
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    • 2009
  • In order to develop probiotic strain for pigs, Lactobacillus spp. (527 isolates), Streptococcus spp. (95 isolates) and Bifidobacterium spp. (25 isolates) were isolated from the feces of 35 pigs. These isolates were tested through in vitro experiment such as acid tolerance at pH 2.0 (Lactobacillus spp. and Streptococcus spp.) or pH 3.0 (Bifidobacterium spp.), bile tolerance in MRS broth containing 0.3% (w/v) Oxgall, heat resistance at $70^{\circ}C$ and $80^{\circ}C$ for 5 min, antibiotic resistance, antimicrobial activity against pathogenic bacteria and Caco-2 cell adherence assay. Finally ten most superior strain (5 Lactobacillus spp. strain, 3 Bifidobacterium spp. strain and 2 Streptococcus spp. strain) were selected as potential candidate for probiotic use in pig industry. It could be used as an alternative to antibiotics in feed additives.

Antibiotic Susceptibility of Bifidobacterium spp. Strains Isolated from Healthy Korean (한국형 유산균 Bifidobacterium 속 균주의 항생물질에 대한 감수성)

  • Chang, Hyun-Ah;Choi, Keum-Hwa;Oh, Tae-Kwon;Kwon, Ae-Ran;Kim, Dong-Hyun;Choi, Eung-Chil
    • YAKHAK HOEJI
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    • v.42 no.6
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    • pp.639-641
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    • 1998
  • Minimal inhibitory concentrations (MICs) of Bifidobacterium spp. strains (Bifidobacterium breve K-110, B. breve K-111 and B. infantis K-525) isolated from healthy Korean against antituberculosis agents and fluoroquinolones were determined. From the MICs it was found that Bifidobacterium breve K-110, B. breve K-111 and B. infantis K-525 were susceptible to rifampicin and fluoroquinolenes and resistant to other antituberculosis agents.

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Platform Technology for Food-Grade Expression System Using the genus Bifidobacterium

  • Park, Myeong-Soo;Kang, Yoon-Hee;Cho, Sang-Hee;Seo, Jeong-Min;Ji, Geun-Eog
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • pp.155-157
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    • 2001
  • Bifidobacterium spp. is nonpathogenic, gram-positive and anaerobic bacteria, which inhabit the intestinal tract of humans and animals. In breast-fed infants, bifidobacteria comprise morethan 90% of the gut bacterial population. Bifidobacteria spp. are used in commericial fermented dairy products and have been suggested to exert health promoting effects on the host by maintaining intestinal microflora balances, improving lactose tolerance, reducing serum cholesterol levels, increasing synthesis of vitamins, and aiding the immune enchancement and anticarcinogenic activity for the host. These beneficial effects of Bifidobacterium are strain-related. Therefore continued efforts to improve strain characteristics are warranted. in these respect, development of vector system for Bifidobacterium is very important not only for the strain improvement but also because Bifidobacterium is most promising in serving as a delivery system for the useful gene products, such as vaccine or anticarcinogenic polypeptides, into human intestinal tract. For developing vector system, we have characterized several bifidobacterial plasmids at genetic level and developed several shuttle vectors between E. coli and Bifidobacterium using them. Also, we have cloned and sequenced several metabolic genes and food grade selection marker. Also we have obtained bifidobacterial surface protein, which will be used as the mediator for surface display of foreign genes. Recently we have succeeded in expressing amylase and GFP in Bifidobacterium using our own expression vector system. Now we are in a very exciting stage for the molecular breeding and safe delivery system using probiotic Bifidobacterium strains.

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Effect of administration of synbiotics mixture containing Bifidobacterium longum and xylooligosaccharide on fecal microbiota and defecation characteristics in healthy volunteers (Bifidobacterium longum과 자일로올리고당을 포함한 synbiotics 섭취가 건강한 성인의 변내 균총과 배변 상태에 미치는 영향)

  • Lee, Jung-Sug;Park, Hyoung-Seop;Kyung, Myungok;Jo, Sung-Eun;Chang, Moon-Jeong
    • Journal of Nutrition and Health
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    • v.53 no.4
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    • pp.390-405
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    • 2020
  • Purpose: The effect of prebiotics intake after administration of a synbiotics mixture (a probiotic, Bifidobacterium longum, and a prebiotic, xylooligosaccharide containing sugar [XOS]) on human intestinal microflora and defecation characteristics was investigated in a randomized controlled trial. Methods: Twenty-five healthy young volunteers (11 males and 14 females) were randomly assigned to 2 groups (BL2XO2 and BL2XO6). The synbiotics mixture was orally administered to both groups for 2 weeks, and the prebiotics were subsequently administered to the BL2XO6 group for 4 additional weeks. The daily dose of the synbiotics mixture comprised 1010 colony-forming unit of Bifidobacterium longum and 10 g of XOS, and during the prebiotics period, the daily dose of prebiotics comprised only 10 g of XOS. The fecal pH, microflora, and defecation characteristics were analyzed at baseline and at weeks 1, 2, 4, and 6. Results: The counts of B. longum and Bifidobacterium spp. in the BL2XO6 group exhibited a steady, increasing trend during the synbiotics and prebiotics periods, whereas those of the BL2XO2 group exhibited considerable variation in each week of the study period. Although there was no significant difference, the counts of fecal Bifidobacterium in the BL2XO6 group tended to be higher than those of the BL2XO2 group at week 6. The growth of Lactobacillus spp. exhibited a time-dependent variation, peaking at week 6 in both groups. Low counts of Clostridium spp. were observed after treatment with the synbiotics and prebiotics in the BL2XO6 group (p < 0.05) throughout the study, whereas the inhibitory effect on Clostridium spp. was maintained only during the synbiotics period in the BL2XO2 group. The defecation characteristics did not differ between the two groups. Conclusion: Administration of XOS after a synbiotics mixture containing B. longum and XOS can exert a prebiotic effect in healthy young volunteers by stimulating Bifidobacteriun spp. growth and inhibiting growth of Clostridium spp.

Protective Effects of Bifidobacterium spp. on Experimental Colon Carcinogenesis with 1,2-Dimethylhydrazine

  • HAN, MYUNG JOO;HAE-YOUNG PARK;DONG-HYUN KIM
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.368-370
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    • 1999
  • The protective role of Bifidobacterium spp. (B. breve K-110, B. breve K-111, and B. infantis K-525) isolated from the fecal samples of healthy Koreans was investigated on 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci(ACF) formation in mouse colon. In mice fed normal diet with DMH treatment, an average of 68.5 ACF/colon was formed, whereas in mice administered with B. breve K-110, B. breve K-111, and B. infantis K-525, the numbers of DMH-induced ACF decreased to 7.2, 10.9, and 6.6 ACF/ colon, respectively. The mean number of crypts/focus was not significantly altered. Fecal harmful enzymes, such as β-glucuronidase, tryptophanase, and urease, were effectively inhibited during the administration of these bifidobacteria to mice. These results suggest that bifidobacteria could prevent colon cancer.

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A Micromethod for Rapid and Simple Isolation of Genomic DNA from Small Scale Culture of Bifidobacterium (소량의 Bifidobacterium 배양액에서 genomic DNA 추출을 위한 신속/간단한 방법)

  • Jeakal, Soo;Park, Hee-Kyung;Song, Ji-Eun;Heo, Tae-Ryeon;So, Jae-Seong
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.781-783
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    • 1995
  • A method is described for the rapid and simple isolation of genomic DNA from 3 ml culture of Bifidobacterium. The method is expected to be used in gene manipulation of Bifidobacterium spp. The isolated DNA using this method is shown to be an excellent substrate for restriction endonuclease digestion and ligation with T4 DNA ligase.

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Screening of Bifidobacterium spp. for the Development of Infant Probiotics (유아용 생균제 개발을 위한 Bifidobacterium spp.의 선발)

  • Yang, Hyun-Ju;Jang, Keum-Il;Kim, Chung-Ho;Lee, Yoon-Bok;Sohn, Heon-Soo;Kim, Kwang-Yup
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.790-794
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    • 2004
  • Bifidobacterium spp. exhibits the highest number of counts among species of microflora in breast-feeding infant intestines and has been used as probiotics. From infant groups with different diets, 42 Bifidobacterial strains were isolated by selective plate, Gram-staining, and morphology using method of Mitsuoka, among which seven isolates were identified as Bifidobacterium spp. by F6PPK test, MIDI, and PCR. B. bifidum PBH-30, selected for development of probiotics, showed high resistance against low pH and oxgall treatment, and inhibition against pathogens such as Salmonella typhimurium and Staphylococcus aureus. B. bifidum PBH-30 could be applicable to dairy products as probiotic strains due to its excellent growth in raw milk.

Antidiarrheal, Anticostive and Antimutagenic Effects of Bifidobacterium breve K-110, K-111 and B. infantis K-525 from Korean in Experimental Animals (한국형유산균인 Bifidobacterium breve K-110, K-111 및 B. infantis K-525 균주의 완하, 항사하 및 항돌연변이효과)

  • Rhee, Young-Kyung;Kim, Dong-Hyun;Han, Myung-Joo
    • Korean Journal of Food Science and Technology
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    • v.31 no.2
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    • pp.547-552
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    • 1999
  • Antidiarrheal, anticostive and antimutagenic effects of Bifidobacterium breve K-110, K-111 and B. infantis K-525 isolated from Korean were investigated in experimental animals. These Bifidobacteria were not significantly affected on the transport of barium sulfate in the small intestine. However, these Bifidobacteria significantly stimulated the transport of barium sulfate in the large intestine. Particularly, when Bifidobacterium breve K-110 (500 mg/kg) was orally administered, the transport of barium sulfate in the large intestine was increased 45%, compared to the control group. On the castor oil-induced diarrheal mice, Bifidobacterium breve K-111 had the antidiarrheal activity but the other Bifidobacteria did not had it. When the antimutagenicity of these Bifidobacteria and their peptidoglycans were examined using Salmonella typhimurium TA98/TA100 in an in vitro assay system, these Bifidobacteria and peptidoglycans showed inhibitory effect of $20{\sim}80%$. These results indicate that Bifidobacterium spp. had antidiarrheal, anticostive and antimutagenic activities as well as the inhibitory activity of harmful enzymes of intestinal bacteria in the intestine.

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Rapid Identification of Bifidobacteria in Dairy Products by Gene-targeted Species-specific PCR Technique and DGGE

  • Hong, Wei-Shung;Chen, Ming-Ju
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.12
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    • pp.1887-1894
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    • 2007
  • In this paper, a rapid and reliable gene-targeted species-specific polymerase chain reaction (PCR) technique based on a two-step process was established to identify bifidobacteria in dairy products. The first step was the PCR assay for genus Bifidobacterium with genus specific primers followed by the second step, which identified the species level with species-specific primer mixtures. Ten specific primer pairs, designed from nucleotide sequences of the 16-23S rRNA region, were developed for the Bifidobacterium species including B. angulatum, B. animalis, B. bifidum, B. breve, B. catenulatum, B. infantis, B. longum, B. minimum, B. subtile, and B. thermophilum. This technique was applied to the identification of Bifidobacterium species isolated from 6 probiotic products, and four different Bifidobacterium spp. (B. bifidum, B. longum, B. infantis, and B. breve) were identified. The findings indicated that the 16S-23S rDNA gene-targeted species-specific PCR technique is a simple and reliable method for identification of bifidobacteria in probiotic products. PCR combined with Denaturing Gradient Gel Electrophoresis (DGGE) for identification of the bifidobacteria was also evaluated and compared with the gene-targeted species-specific technique. Results indicated that for fermented milk products consistency was found for both species-specific PCR and PCR-DGGE in detecting species. However, in some lyophilized products, the bands corresponding to these species were not visualized in the DGGE profile but the specific PCR gave a positive result.