• Title, Summary, Keyword: Bisroot

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Effects of Bisroot in the Diet on Growth, Body Composition, Immume responses of Nile Tilapia, Oreochromis niloticus (사료 중 비스루트가 나일틸라피아, Oreochromis niloticus의 성장, 체조성 및 면역 방응에 미치는 영향)

  • 김용화;오승용;황미혜;조재윤;박수일;김유희;윤길하;박정환
    • Journal of Aquaculture
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    • v.11 no.4
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    • pp.495-503
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    • 1998
  • The present study investigated the effects of Bisroot, that contains live bacteria (Bacillus polyfermenticus, Bacillus mesentericus, Streptococcus faecalis, & Bifidobacterium breve) and digestive enzymes (protease, lipase), on the growth, body composition and immune response of Nile tilapia fingerlings. One percent of the Bisroot was added to the experimenta feed. All exprimental fish were fed for 60 days. The weigh gains among the experimental fish were not significntly different (P>0.05). Hematocrit value, hemoglobin, total protein, glucose, GOT, and GPT were unaffected by Bisroot treatment. However, it was observed that glucose, GOT, and GPT value in the fish that were fed Bisroot, were lower than the control. The complement activity ($CH_50$) tended to be significantly increased by Bisroot treatment, but not lysozyme activity. Phagocytosis and respiratory burst activities of macrophages in the head kidney were enhanced by Bisroot. Therefore, the Bisroot diet enhances the cellular immune activities were enhanced by Bisroot. Therefore, the Bisroot diet enhances the cellular immune activities of non-specific immune responses. When fish were challenged with a virulent strain of Edwardsiella tarda, the Bisroot treated fish were more resistant than the control. The present results suggest that the introduction of Bisroot into the diet of Nile tilapia could increase their resistance against bacterial infection, reduce fish mortality, and offers economic benefits.

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Antibacterial Activities of B. polyfermenticus SCD Against Pathogenic Bacteria and Effects on Animals and Humans (Bacillus polyfermenticus SCD의 병원성 세균에 대한 항균성과 동물 및 임상에 미치는 영향)

  • 강재선;전경동;김원석;조우성;권주열;문경호
    • YAKHAK HOEJI
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    • v.48 no.1
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    • pp.70-74
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    • 2004
  • Bacillus polyfermenticus SCD which is commonly called as Bisroot (equation omitted) has been appropriately used for the treatment of long-term intestinal disorder's. This strain strongly inhibited against methicillin resistance Staphylococcus aureus (MRSA) and various pathogenic microorganisms. Effects of B. polyfermentius SCD administration on death rates and egg-laying rates in two groups of hens were investigated. This strain decreased the death rates of two groups by 16.26% and 11.72%, respectively. Also this strain increased the egg-laying rates of those 2.74% and 2.66%, respectively Clinical tests of B. polyfermenticus SCD administration to healthy adults showed not adverse effects but decreased glyceride concentration from 154.52 mg/dl to 135.41 mg/dl after two week administrations.

Characterization of Bacillus polyfermenticus SCD for Oral Bacteriotherapy of Gastrointestinal Disorders (식품용 프로바이오틱 비스루트균의 주요 생균제 특성)

  • Paik, Hyun-Dong;Jung, Min-Yong;Jung, Hwang-Young;Kim, Won-Seok;Kim, Kee-Tae
    • Korean Journal of Food Science and Technology
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    • v.34 no.1
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    • pp.73-78
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    • 2002
  • Bacillus polyfermenticus SCD, which is commonly called as Bisroot strain, is being used for functional foods through the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine in both humans and animals. The cells of B. polyfermenticus SCD were treated for 24 h in artifical bile after incubation for 2 h in artificial gastric juice and final number of the strain was reached to around $3.3{times}10^7\;CFU/mL$. In test of API ZYM kit, ${\beta}-glucuronidase$ or ${\beta}-glucosidase$ was not produced by B. polyfermenticus SCD. B. polyfermenticus SCD was resistant to antibiotics, such as nisin, streptomycin, tetracycline, and rifamycin. B. polyfermenticus SCD was also affected by alcohol concentration up to 4%, but more than 8%, their growth was not affected significantly. Finally, B. polyfermenticus SCD was shown to inhibit the growth of Listeria monocytogenes ATCC 19111 completely within 24 h of incubation, which indicated its bactericidal nature.

Chayacterization of Bacillus polyfermenticus SCD as a Probiotic. (Bacillus polyfermenticus SCD의 생균제로서의 특성)

  • 전경동;김혜진;이광호;백현동;강재선
    • Microbiology and Biotechnology Letters
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    • v.30 no.4
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    • pp.359-366
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    • 2002
  • Bacillus polyfermenticus SCD which is commonly called as Bisroot strain is being used for functional foods through the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine in humans. The cells of B. polyfermenticus SCD were treated for 4h in artificial gastric juice (pH 2.0,3.0) and bile acid. Final viability of the strain in artificial gastric Juice (pH 2.0, 3.0) is reached to 62.8% and 81.2% respectively B. polyfermenticus SCD is resistant to antibiotics such as streptomycin, rifampicin, nystatin and ampicilin. B. polyfermenticus SCD is well known supplies the nutrients by synthesizing vitamin $B_1$, $B_2$, C and K. B. polyfermenticus SCD produces various digestive enzymes and the enzymes enable to completely digest diets in our body. Above all, $\alpha$-amylase and pretense activities are very higher than B. subtilis KCTC 1020, about two fold and twenty five fold respectively. B. polyfermenticus SCD is very stable during long-term storage period in phosphate buffers of wide-range pH, solutions of various concentrations of sodium chloride, 5% glucose solution and water.

Increased Stability of Bacillus polyfermenticus SCD in Low pH, High Temperature and High Glucose Concentration via Three Layer Coating (Bacillus polyfermenticus SCD의 Three Layer Coating에 의한 pH, 열, 높은 glucose 농도에 대한 안정성효과)

  • 이진옥;전경동;강재선;이재화
    • KSBB Journal
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    • v.19 no.3
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    • pp.221-225
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    • 2004
  • Bacillus polyfermenticus SCD derived from Bacillus sp., which is commonly called as Bisroot$^{ⓡ}$. The goal of this study, is to Increase stability of Bacillus polyfermenticus SCD in low pH, high temperature and high glucose concentration via three layer coating. The viability of coated Bacillus polyfermenticus SCD increased to 30%, 20%, 14% in the condition of pH 2 4 6 than that of uncoated Bacillus polyfermenticus SCD at 37$^{\circ}C$ for 4 h. Final viability of the coated Bacillus polyfermenticus SCD in 80$^{\circ}C$ increased to 40% than that of uncoated Bacillus polyfermenticus SCD. In high glucose concentration coated Bacillus polyfermenticus SCD is more stable than uncoated about 50%. In conclusion, the three layer coated Bacillus polyfermenticus SCD is very stable for low pH, high temperature and high glucose concentration.

Development of a Culture Medium for Growth and Sporulation of Bacillus polyfermenticus SCD (프로바이오틱 비스루트균의 아포생산을 위한 최적배지 개발)

  • Lee, Kwang-Ho;Park, Kyu-Yong;Kim, Seong-Mi;Kim, Won-Seok;Paik, Hyun-Dong
    • Korean Journal of Food Science and Technology
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    • v.34 no.2
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    • pp.263-268
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    • 2002
  • Bacillus polyfermenticus SCD, which is commonly called a 'Bisroot' strain, has been appropriately used for the treatment of long-term intestinal disorders, since the live strains, in the form of active endospores, can successfully reach the target intestine. Goal of this study was to develop an industrial medium for growth and sporulation of B. polyfermenticus SCD. From the results of effect of mixed carbon sources on growth and sporulation of B. polyfermenticus SCD, glucose 2% and starch 2% was particularly found to be the most effective for the maximum number of spore production, resulting in spore cells of $4.3{\times}10^9\;spores/mL$ with a sporulation yield of 91%. For the effect of nitrogen sources, the maximum spore cells of $5.7{\times}10^9\;spores/mL$ of B. polyfermenticus SCD with a sporulation yield of 97% was obtained when B. polyfermenticus SCD was cultivated in an optimum nitrogen source medium containing 5% soybean flour. A medium involving proper phosphate salt yielded the maximum number of a spore cells of $6.0{\times}10^9\;spores/mL$ with a sporulation yield of 95%. Finally, the efficacy of an industrial medium (KH5 medium) on growth and sporulation of B. polyfermenticus SCD was investigated in jar fermenter. The higher number of viable cells $(3.3{\times}10^{10}\;cells/mL)$ and spore cells $(3.0{\times}10^{10}\;spores/mL)$ were obtained in 5 L fermenter when compared with a 500 mL baffle flask cultivation. Thus, KH5 medium developed in this study shows promise as an industrial medium because of higher cells and sporulation yield.