• Title/Summary/Keyword: Bombyx mori

Search Result 980, Processing Time 0.092 seconds

The Whitening Effect by Water Extracts of Bombyx Mori Linne (백강잠(Bombyx Mori Linne) 물추출물의 미백 효능)

  • Ann, Young-Hee;Choi, Jeung-Sook
    • Journal of the Korean Society of Fashion and Beauty
    • /
    • v.4 no.4 s.10
    • /
    • pp.81-86
    • /
    • 2006
  • Numerous novel ingredients have been introduced for the hight functionality of whitening cosmetics. Tough the preliminary research, we have found water extracts Bombyx mori have high whitening efficacy. The results of the research for the whitening effect of Bombyx Mori L. are as follow 1. Bombyx Mori L. inhibited concentration dependently the generation of melanin increased by the stimulation of $\alpha$-MSH and protoporphyrin IX, and $IC_{50}$ value was 8.3, 9.2 ${\mu}M$ respectively. 2. Melanin increased by the stimulation of $\alpha$-MSH and protoporphyrin IX was five to seven times superior in the inhibiting effect, compared with kojic acid used as positive control group. 3. Bombyx Mori L. did not have a decolorizing effect on melanin already generated. 3. Bombyx Mori L. was observed to have toxicity of over 100 ${\mu}M$ for the mouse melanoma B16 cells. Therefore, These results suggest that water extracts of Bombyx mori have inhibitory activity against mushroom tyrosinase and inhibitory activity of melanin synthesis in B16 melanoma cells in vitro.

  • PDF

Effect of the Ecdysteroid UDP-Glucosyltransferase Gene of the Bombyx mori Nucleopolyhedrovirus on the Development of the Silkworm, Bombyx mori (누에 핵다각체병 바이러스의 Ecdysteroid UDP-glucosyltransferase 유전자가 누에의 발육에 미치는 영향)

  • ;;Shizuo George Kamita
    • Journal of Sericultural and Entomological Science
    • /
    • v.40 no.2
    • /
    • pp.105-110
    • /
    • 1998
  • The baculovirus egt gene encodes an ecdysteroid UDP-glucosyltransferase(EGT) which catalyzes the transfer of glucose from UDP-glucose to the insect moltion hormone ecdysteroid resulting in a functionally inactive ecdysteroid. In baculovirus-infected insect larvae, EGT has been shown block molting and pupation. In this study, we compared the development of 4th and 5th instar silkworm, Bombyx mori, larvae injected with either wild-type bombyx mori nucleopolyhedrovirus (BmNPV) or a mutant BmNPV(BmEGTZ) in which the egt gene was disrupted by the insertion of a lacZ gene cassette. Larvae injected with BmEGTZ died roughly 12 h more rapidly compared to indentical larvae infected with BmNPV. In addition, BmEGTZ- infected larvae prematurely stopped feeding and gain less weight compared to BmNPV-infected larvae. In order to investigate why BmEGTZ-infected larvae died more rapidly than BmNPV-infected larvae, the array of hemolymph proteins in BmEGTZ-or BmNPV-infected larvae were analyzed by SDS-PAGE. The hemolymph of BmEGTZ-infected larvae showed virus-specific proteins, including polyhedrin, about 12 h earlier than the hemolymph of BmNPV-infected larvae

  • PDF

Identification of Dopa decarboxylase associated protein from Bombyx mori

  • Hwang, Jae-Sam;Kim, Sung-Kuk;Kang, Seok-Woo;Goo, Tae-Won;Yun, Eun-Young;Park, Kwang-Ho;Chang, Jong-Soo
    • Proceedings of the Korean Society of Sericultural Science Conference
    • /
    • 2003.10a
    • /
    • pp.97-98
    • /
    • 2003
  • Current study was aimed to understand an interaction between Dopa decarboxylase (DDC) and proteins that specifically binds to DDC in the silkworm, Bombyx mori. Materials and Methods: Materials-Animal: Bombyx mori Construction of GST fusion protein Preparation of lysates: Protein extracted from whole body of Bombyx mori Methods-In vitro binding assay with lysates, Peptide sequence and RACE-PCR (omitted)

  • PDF

A Screening System for Environmental Mutagens by Means of Specific Locus Mutation of the Silkworm, Bombyx mori (누에의 특정좌위 돌연변이를 이용하는 변이원 검색계의 피검누에계통 선정 및 변이원 감수성)

  • 김삼은;안미영;원형주;김종길;최지영
    • Toxicological Research
    • /
    • v.16 no.1
    • /
    • pp.53-57
    • /
    • 2000
  • A mutagenicity testing system using a specific locus mutation of Bombyx mori was introduced from National Institute of Genetics in Japan. In the system, mutagenicity could be detected by the egg color manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. The efficiency to detect mutagenicity of the system was examined and improved. To promote the sensitivity of the system to mutagens, eight varieties of Bombyx mori were tested for their sensitivity to two mutagens, EMS and MMC. Two varieties of the silkworm, N12 and C5, were finally selected as the most sensitive ones. The most sensitive development stage of the silkworm to mutagens was mid and late pupal stages for female and male, respectively. The system will be applied to test unknown mutagens after some more detail examination about its sensitivity.

  • PDF

Prevention of Diapause in Bivoltine Eggs of the Silkworm, Bombyx mori, L., through a Cross with the Race KS-10 as Male Parent

  • Mundkur, Rajendra;Murthy, Mallesha;Mahadevappa;Raghuraman, R.;Bongale, U.D.
    • International Journal of Industrial Entomology
    • /
    • v.9 no.1
    • /
    • pp.107-109
    • /
    • 2004
  • The present investigation reports a phenomenon hitherto unknown in tropical sericulture, wherein dia-pause nature of bivoltine eggs is overcome through a cross with a non-diapausing race of silkworm, Bombyx mori, L. Eggs of bivoltine silkworm Bombyx mori, L. generally do not hatch under tropical conditions. To prevent diapause, they are subjected to acid treatment or low temperature hibernation scheduled. A race developed at KSSRDI is found to prevent the diapause nature of bivoltine eggs when crossed as male parent, without any acid treatment or hibernation schedule. This phenomenon is reported for the first time, being unique, opens up interesting area of research in silkworm genetics of commercial implications in the industry.

Characteristics and pathogenicity of the Entomopathogenic Fungus Beauveria bassiana101AA on the silkworm (Bombyx mori) (백강균(Beauveria bassiana)101A의 특성 및 가잠(Bombyx mori)에 대한 병원성 검정)

  • 정이연;남성희;조세연
    • Journal of Sericultural and Entomological Science
    • /
    • v.42 no.2
    • /
    • pp.99-103
    • /
    • 2000
  • This study was carried out to investigate incubating characteristics, LTSEM observation, nature of a disease of infected silkworms(bombyx mori), LD$\_$50/, optimum infective condition for mass production and infection percentage(%) on conservation periods. On the V8 media, the colony was 0.5∼2.0cm/8 days in diameter and white or slighty colored with a white fluffy to powdery appearance. The conidia was subglobos in shape, zig-zag appearance, 1-celled, hyaline and 3.2$\times$2.4 ㎛ in size on the average. The conidiophore was irregular grouped, hyaline, rounded or flask-shaped. The LD$\_$50/ values of the 2nd and 4th silkworms were each other 6.6142(Log) in natural temperature and humidity (25$\^{C}$, 65%). On optimum infective condition for mass production, all of preservation time(hr.) postinoculation in 1.0$\times$10$\^$8/ conidia/㎖ was over 97% and only 20 hr in 1.0$\times$10$\^$7/ condia/㎖ was over 90%.

  • PDF

Biochemical Properties of the Lectin Isolated from Bombyx mori (누에(Bombyx mori)로부터 분리한 렉틴의 생화학적 특성)

  • Kim, Se-Jin;Lee, Sang-Yong;Jeune, Kyung-Hee
    • Korean Journal of Pharmacognosy
    • /
    • v.42 no.1
    • /
    • pp.68-75
    • /
    • 2011
  • A new lectin was purified from Bombyx mori (BML) by physiological saline extraction, ammonium sulfate precipitants, anion exchange column chromatography on DEAE Sephadex A-50 and gel filtration column chromatography on Sephadex G-200. BML agglutinated trypsinized and glutaraldehyde-fixed erythrocytes, and was observed the most high activity with rabbit, chicken erythrocytes and rat splenic lymphocytes. Agglutinability was markedly affected at highly acidic pH, but was relatively stable with high temperature. The effect of metal ions was observed and BML was affected by bivalaent cations, especially depending on $Ca^{2+}$, $Fe^{2+}$, $Mn^{2+}$, whereas, inhibited by $Mg^{2+}$. Agglutination was strongly inhibited by heparin and glucuronic acid. BML was proved to be a glycoprotein which contains 17.16% of sugars. By mass spectrometry analysis, we found 2 bands that were considered as lectin subunits.

Cloning and Characterization of hydroxypyruvate isomerase (EC 5.3.1.22) gene in silkworm Bombyx mori

  • Lv, HongGang;Chen, KePing;Yao, Qin;Wang, Lin
    • International Journal of Industrial Entomology
    • /
    • v.17 no.2
    • /
    • pp.189-195
    • /
    • 2008
  • The sequence of hydroxypyruvate isomerase gene was obtained in NCBI. In this study, the hydroxypyruvate isomerase gene of Bombyx.mori was identified and annotated with bioinformatics tools. The result was confirmed by RT-PCR, prokaryotic expression, mass spectrographic analysis and sub-cellular localization. The hydroxypyruvate isomerase cDNA comtains a 783bp ORF, and has 4 exons. The deduced protein has 260 amino acid residues with the predicted molecular weight of 29169.30 Da, isoelectric point of 6.10, and contains conserved PRK09997 and Hfi domains. The hydroxypyruvate isomerases of Nasonia vitripennis and Bombyx mori have a high homology. Through RTPCR analysis, we found that this transcript was present in testis, ovary, blood-lymph, fat body, midgut, silk gland and tuba Malpighii. This protein was located in cytoplasm through immunohistochemistry. We submitted the cloned gene under the accession number EU344910. The enzyme has been classified under accession number EC 5.3.1.22.

Molecular Phylogenetics of Silkworm (Bombyx mori) Based on Mariner-Like Elements (MLEs) (Mariner-Like Elements (MLEs)를 이용한 누에의 분자적 계통 분석)

  • 황재삼;이진성;김영섭;성연문
    • Journal of Life Science
    • /
    • v.9 no.2
    • /
    • pp.176-181
    • /
    • 1999
  • In order to understand molecular phylogenetics of silkworm (Bombyx mori), we analyzed the sequences of BmoMAR isolated from Bomhyx mori that is partial coding gene of transposase of mariner-like element(MLE). By pairwise comparing nucleotide sequences of BmoMAR with ten previously reported insect MLEs accessed in GeneBank, the average genetic distance was estimated to be 0.4840. The phylogenetics tree constructed from nine insect species except for human MLE(Hsmarl) by UPGMA method indicated that MLEs are divided into three clusters, and Drosophila mariutiana was independently subgrouped. Bombyx mori(BmoMAR) was subgrouped with microcaddishfly (Orthotrichia cristata), webworm(Atteva punctella), almond moth(Ephestia cautella), Hyalopora cecropia which we lepidoptera. Phylogenetics tree according to UPGMA principle, on the basis of informative nucleotide sequences of nine insect MLEs, indicated that Bombyx mori was more closely related to microcaddishfly(Orthotrichia cristata) and webworm (Atteva punctella) of lepidoptera. We suggest that insect MLEs are a useful key for studying molecular phylogenetics among intra species of insects.

  • PDF