• Title, Summary, Keyword: Bottle cultivation

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Selection of an Substitute Sawdust Material in Pleurotus ostreatus by Bottle Cultivation (느타리버섯 병재배의 톱밥 대체 배지 재료 선발)

  • Jang, Myoung-Jun;Lee, Yun-Hae;Ju, Young-Cheol
    • The Korean Journal of Mycology
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    • v.38 no.2
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    • pp.142-145
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    • 2010
  • In this study, we attempted to find substitute materials, swelling rice hull, cocopeat, corncob and coconut sawdust, for sawdust in bottle cultivation of Pleurotus ostreatus. Chemical characters of mixture substrates with four substitute materials for pine sawdust were not different significantly. By comparison in mycelial growth and yield of fruitbody, mixture substrate of cocopeat were showed the same level in P. ostreatus. Therefore, it is suggested that cocopeat was substituted for sawdust for cultivation of P. ostreatus.

Cultural characteristics of Pholiota adiposa according to substrates composition of sawdust medium by bottle cultivation (검은비늘버섯의 병재배 시 톱밥배지 조성에 따른 재배적 특성)

  • Kim, MIn-Ja;Chang, Who-Bong;Lee, Kwan-Woo;Joo, Gyeong-Nam;Kim, Yee-Gi
    • Journal of Mushroom
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    • v.13 no.1
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    • pp.21-25
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    • 2015
  • This study was carried out to develop optimum sawdust medium for bottle cultivation of Pholiota adiposa. Five kinds of sawdust media were tested. Period of primordial formation was shorter 1-5 days in T3 and in T4 than in other treatments. Total cultivation period was 3-5 days longer in treatments including oak sawdust than in T5, treatment of poplar+rice bran(8:2). Stipe diameter, stipe length, and number of valid stipe were the biggest in T3. Yield of fruiting body was increased 33% in T3 and 12% in T4, respectively compared to T1 as control. Therefore, T3, treatment of oak sawdust+beet pulf+cottonseed meal(5:3:2), would be appropriate for the commercial production of Pholiota adiposa by bottle cultivation.

Cultural characteristics and consumer acceptance of Pleurotus pulmonarius (산느타리버섯 재배특성 및 기호도 조사)

  • Lee, Kwang-Jae;Kim, Kyoung-Hee;Cho, Byung-Ju;Park, Young-Hak
    • Journal of Mushroom
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    • v.6 no.3_4
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    • pp.146-149
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    • 2008
  • Pleurotus pulmonarius usually grows on the dead wood of deciduous trees in mid summer to early fall. The pileus color is white to tan and fruiting body is generally smaller than P. ostreatus. This study was carried out to investigate the characteristics of cultivation and consumer acceptance of P. pulmonarius. The period of incubation and primordial occurrence were required about 33days, 5days in bottle cultivation repectively, and the yield was 170g/850cc bottle. In bed cultivation, the period of primordial occurrence was similar to that of bottle cultivation and the yields was $35.7{\sim}46.2kg/3.3m^2$. Taste and favor of P. pulmonarius were superior to P. oostreatus., but the appearance, color and shape, was slightly inferior to P. ostreatus.

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Occurrences of Major Mushroom Diseases and Microbial Densities of Mushroom Cultivation Facilities (국내 주요 버섯류의 병해 발생과 재배사의 미생물 밀도 조사)

  • An, Yu-Na;Jang, Bo-Ra;Kim, Myun-Su;Weon, Hang-Yeon;Jhune, Chang-Sung;Cheon, Se-Chul
    • The Korean Journal of Mycology
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    • v.37 no.2
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    • pp.144-149
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    • 2009
  • The occurrences of the major diseases and the densities of air-born microbes were surveyed in the cultivation facilities for oyster mushroom (Pleurotus ostreatus), king oyster mushroom (Pleurotus eryngii), and enoki mushroom (Flammulina velutipes) in different areas of Korea. Green mold disease was most often developed in oyster mushroom bed cultivation with the disease incidence rate of approximate 10% while the disease incidences from bottle and plastic envelop cultivation were less than 1~2%. In the bed cultivation, the major air-born microbes in the growth room were Aspergillus, Penicillium, Trichoderma, and Curvularia with the total fungal population density of 567~1,297 CFU/$m^3$ . However, only Trichoderma and Penicillium were detected in the growth rooms and innoculation rooms of bottle and plastic envelop cultivation with the densities of 350~700 CFU/$m^3$ and 160~260 CFU/$m^3$, respectively. The bacterial diseases become evident in the growth rooms of bottle and plastic envelop cultivation with the approximate incidence rate of 10%. The identified bacterial species were Brevibacillus levelkil, Rhizobium radiobacter, Brevundimonas vesicularis, Pseudomonas mosselii, Microbacterium testaceum. Sphingomonas panmi, Sphingomonas yabuuchiae, Paracocus dinitrificans, Curtobacterium flaccumfaciens pv. flaccumfaciens and some unidentified bacteria with the densities of 40~6,359 CFU/$m^3$ in the growth rooms and 9 CFU/$m^3$ in the inoculation room. This study indicated that the green mold disease by fungal strains was the major mushroom disease in the bed cultivation and suggested that the contamination of bacteria and fungi together in the growth media could result in severe production loss. The plastic envelope and bottle cultivation were evidenced to be less susceptible to such contaminations.

Methods of temperature measurement of medium in bottle during sterilization (병재배 배지살균 시 배지내부의 실시간 온도측정 방법)

  • Cheong, Jong-Chun;Jhune, Chang-Sung;Lee, Chan-Jung;Oh, Jin-A;Shin, Pyeong-Gyun
    • Journal of Mushroom
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    • v.9 no.2
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    • pp.74-79
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    • 2011
  • This study was carried out to develop a method related to measuring the temperature of sterile medium in bottle cultivation. When the medium is sterilized, the device is able to be inserted inside of the medium and the temperature can be directly measured in real time although high temperature and pressure are detected in the sterilization. This device can be able to measure the sterilization temperature in short intervals inside of autoclave and medium used in bottle cultivation. As the method were applied to the field cultivation of mushroom, we could produce mushroom in consistent manner through the optimum sterilization of the medium.

Cultivation of Oyster Mushrooms Using the Garlic Peel as an Agricultural by-product (마늘껍질을 이용한 느타리버섯의 인공재배)

  • Lee, Sang-Sun;Kim, Soon-Keun;Lee, Tae-Soo;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.25 no.4
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    • pp.268-275
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    • 1997
  • High prices of raw materials used as media for the mushroom cultivation increased the cost of commercial production of oyster mushroom (Pleurotus ostreatus). In this study, garlic peels (Allium sativum f. pekinese) as an agricultural by-product were investigated to replace the saw dust for the bottle cultivation of oyster mushroom. Mycelial growth of oyster mushroom were examined by the extracts made from the sawdust, rice bran and garlic peels. The mycelial growth was very poor in the agar media containing the extracts of sawdust or garlic peels, but was good when those of the rice bran were added. In the polypropylene bottle experiment, the sawdust medium which ammended with minerals vitamin was essential for the production of the mushroom fruitbodies. The rice bran was considered to stimulate the mycelial growth, but not the development of basidiocarps. The garlic peel was not a factor to stimulate the production of mushroom fruitbodies, but a raw material ammended with the rice bran produced much amounts of mushroom. In this work, garlic peels ($10{\sim}70%$ v/v) added to the mixture of sawdust and rice bran (4 : 1, v/v) was considered to help the productions of mushroom fruitbodies. Based on the result, the replacement of expensive saw dust with inexpensive garlic peels was a good example to reduce production cost of the bottle cultivation of mushroom.

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Comprehensive model formedium composition for mushroom bottle cultivation (버섯 병재배를 위한 배지조성물의 종합관리 모델)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Moon, Ji-Won
    • Journal of Mushroom
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    • v.14 no.3
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    • pp.111-118
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    • 2016
  • The medium used for bottle cultivation of mushrooms is made according to a specific workflow; this includes mixing the raw materials, adjusting the moisture content, placing the mixture into bottles, and sterilizing and cooling the medium. Medium composition should be kept constant throughout the year. placed in the bottle, choice of materials, moisture content, weight of the medium, bulk density, nitrogen content, three-phase medium, such as suitable conditions. To achieve constant medium composition a comprehensive model has been established in accordance with the technology developed in the work process with respect to medium compositions piecemeal. By comprehensively applying the various methods to establish medium composition, our country can expect to grow at over four seasons and overcome the many difficulties in mushroom mycelial growth periods, cultivation periods, quantity, and quality.

Sawdust Cultivation of Lentinula edodes Using a Detachable Plastic Bottle (분리형 용기를 이용한 표고버섯 톱밥재배 연구)

  • Jeong, Yeun Sug;Jang, Yeongseon;Ryoo, Rhim;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.385-392
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    • 2019
  • Currently, plastic bags are being used for sawdust cultivation of shiitake mushroom. However, due to serious environmental problems caused by the use of plastic bags, we studied the efficacy of bottle cultivation method to replace the sawdust bag method. Small detachable plastic bottles (400 g capacity) filled with Quercus spp. sawdust and wheat bran (4:1 w/w) media were incubated for 80 and 120 days. The weight loss (%) of the media was higher for the NIFoS 2464 strain at an approximate light intensity of 300 Lux than light intensity of 500 Lux; the light intensity was associated with the loss of sawdust medium-weight during the cultivation period. The highest yield was observed when the strain was cultivated for 80 days under dark conditions, 40 days under 500 Lux light, and air circulation fan speed of 30 rpm. When incubated for 120 days, mushroom yield in the bottle media was higher at 40 days of light exposure than 20 days of light exposure. In the bottle media incubated for 80 days under dark conditions, the mushrooms fruited due to repetitive water spraying on the top of the media and light stimulation during the fruiting period. The media could be separated from the bottles because the media shrank after the first harvest. These separated plastic bottles could be re-used for mushroom cultivation, thereby reducing the amount of plastic waste.

Physiological activitive of Grifola frondosa by log cultivation and bottle cultivation (원목재배와 병재배 잎새버섯(Grifola frondosa)의 생리활성)

  • Kim, Jong-Bong;Seo, Hye-Young
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.185-191
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    • 2015
  • This research was carried out to determine the differences of physiological activites between Grifola frondosa of log cultivation(LC) and Grifola frondosa of bottle cultivation(BC). Total flavonoids content, total phenolics content, electron donating ability(EDA), nitrite-scavenging ability(NSA), SOD-like activity and inhibitory effect of Xanthine oxidase were examined. The highest value of total flavonoid content is $5.96{\pm}0.81mg/g$ in water extract from Grifola frondosa of log cultivation at $40^{\circ}C$ (LC-W40) but, one of total phenolics compound is $44.53{\pm}0.89mg/g$ in water extract from Grifola frondosa of bottle cultivation at $40^{\circ}{\cdots}$ (BC-W40). The EDA using DPPH of BC-W40 extract showed the highest value of $97.14{\pm}0.71%$. Nitrite-scavenging ability was $62.55^{\circ}{\ae}0.36%$ in extract from Grifola frondosa of BC-W40 at pH 1.2. The value was SOD-like activity showed the highest value of $18.95^{\circ}{\ae}1.39%$ in extract from LC-W40. Xanthine oxidase inhibitory activity was the highest value of $54.31{\pm}0.40%$ in extract from Grifola frondosa BC-W40, and dependent on concentrations. These results showed that a the antioxidant effects of Grifola frondosa is excellent. However, physiological activities of Grifola frondosa were not depend on caltivation method regulary, and were different according to kind of solvents, concentraitions and physialogical factors examined such as EDA, SOD-like activity and NSA.

Methods of Agrocybe cylindracea simultaneous cultivation for small scale bottle cultivation farmers of Pleurotus eryngii (소규모 큰느타리 병재배 농가에서 버들송이 동시재배 방법)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Oh, Jin-A;Yoo, Young-Bok
    • Journal of Mushroom
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    • v.9 no.4
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    • pp.161-165
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    • 2011
  • Small scale mushroom growers take a lot of the costs in the bottle cultivation than the large scale growers. Therefore, they are not competitive in the market. Mushroom cultivation in small scale mushroom farm is labor-intensive and this experiment was carried out to establish the method for the cultivation of various kinds of mushrooms together under the similar conditions in the production system. As a result, the common medium were selected based on the growing conditions of Pleurotus eryngii and Agrocybe cylindracea, and the level of medium moisture content were studied. The results were shown below. When the medium in the input stage for bottle cultivation was filled by using automatic machine, the range of the three state rates in the bottle is different depending on ingredients and the mixing ratio. The optimum moisture content of the medium for some mushroom production was a different trend based on the medium cluster of the raw materials. The optimum moisture content for P. eryngii in the medium was 70% (douglas fir sawdust, rice bran or sawdust, dried bean curd refuse). In the medium containing douglas fir sawdust, wheat bran was 65%, and the medium including douglas fir sawdust, wheat bran, dried bean curd refuse was 67%. The optimum moisture content of the above three media for A. cylindracea was 70%. The suitable medium for the bottle cultivation of P. eryngii was selected as douglas fir sawdust 75%, wheat bran 20%, dried bean curd refuse 5%, and moisture content 67%. The medium of A. cylindracea was selected as douglas fir sawdust 75%, wheat bran 25%, and moisture content 70%. The suitable medium for bottle cultivation of P. eryngii and A. cylindracea was shown as douglas fir sawdust 75%, rice bran20%, dried bean curd refuse 5%, and 70% moisture content to be used as a common medium of the growing. The incubation period, primordial formation, and growth environment conditions of P. eryngii and A. cylindracea were a similar trend. Therefore, the small scale farmers of P. eryngii can cultivate A. cylindracea together with P. eryngii.