• 제목, 요약, 키워드: Campylobacter jejuni

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Campylobacter jejuni, C. coli, Arcobacter butzleri와 Helicobacter pylori의 PCR에 의한 분리검출 (Selective Detection of Campylobacter jejuni, C. coli, Arcobacter butzleri and Helicobacter pylori by Polymerase Chain Reaction)

  • 이영덕;박종현
    • 한국식품과학회지
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    • v.34 no.6
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    • pp.1134-1139
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    • 2002
  • Campylobacter, Arcobacter, Helicobacter는 분류학적으로 동일한 rRNA superfamily Ⅵ로 식중독 이외에도 위궤양, 위암, 유산 및 신경 장애를 유발한다. Campylobacter, Arcobacter, Helicobacter를 오염된 식품 등에서 선택적으로 검출하기 위해 PCR, multiplex-PCR, RFLP(restriction fragment length polymorphism)의 기법을 이용하였다. Campylobacter, Arcobacter, Helicobacter의 16S rRNA를 target으로 하는 CHA primer를 사용하여 동일한 PCR product의 검출할 수 있었다. C. jejuni와 C.coli를 A. butzleri와 H. pylori로부터 선택적으로 검출하기 위해 fla A gene을 target으로 하는 pg3, p50을 사용하였으며, A. butzleri는 23S rRNA를 target으로 하는 Arco2, Butz를 이용했다. 또한 H. pyloyi는 isocitrate dehydrogenase gene을 target으로 하는 icd1, icd2를 사용하였고, C. jejuni는 ceuE gene을 target으로 하는 JEJ1, JEJ2를 이용하여 효과적으로 분리검출이 이루어졌다. 또한 제한효소 Dde I 을 사용하여 PCR-RFLP를 통해 C. jejuni, C. coli를 A. butzleri, H. pylori로부터 분리할 수가 있었다. 따라서 이러한 primer를 이용하여 C. jejuni, C. coli, A. butzleri, H. pylori가 함께 오염되었을 때 각각 균주의 선택적인 검출이 가능할 것이다.

PCR을 이용한 육류 내 Campylobacter sp. 및 Campylobacter jejuni의 분리 검출 (Selective Detection of Campylobacter sp. and Campylobacter jejuni in Meat Food by Polymerase Chain Reaction)

  • 주종원;홍경포;김용휘;조상범
    • 동아시아식생활학회지
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    • v.18 no.5
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    • pp.753-759
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    • 2008
  • 본 연구는 식품 샘플에서 단시간 내에 간단한 방법으로 Campylobacter jejuni를 검출하기 위하여 10가지의 Campylobacter genus-specific primer와 C. jejuni species-specific oligonucleotide를 제작하였고, amplification efficiency test를 통하여 4종으로 축소한 후 다시 specificity, sensitivity analysis를 통하여 최종적으로 CB4, CJ1 2종의 oligonucleotide primer를 선별하였다. 선별된 oligonucleotide primer는 각각 Campylobacter genus specific, Campylobacter jejuni에 대한 species specific한 특성을 지닌다. 또한, sensitivity analysis를 통하여 isolated colony에서 reaction tube당 $10^0{\sim}10^1$까지의 detection limit을 확보하였다. 육류 시료에서는 Sensitivity가 $10^1{\sim}10^2$으로 떨어지는 양상을 보였으며, 이는 쇠고기나 돼지고기에 존재하는 hemoglobin이나 immunoglobulin 등의 PCR inhibitor의 영향에 의한 것으로 추정된다.

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노변식품에서 분리한 Campylobacter jejuni의 특성에 관한 연구 (Study on Characterizations of Campylobacter jejuni Isolated from Street Vended Foods)

  • 빈재훈;차인호;김용환;하상태;김경숙;권혁동;이채남
    • 한국식품위생안전성학회지
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    • v.9 no.3
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    • pp.117-121
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    • 1994
  • On the purpose of epidemiological survey related to foods poisoning, a total of 316 specimens collected from street vended foods in Pusan area were examined for the presence of Campylobacter Jejuni(C. jejuni) and also the isolation rates, biochemical properties and antibiotic susceptibility were investigated. Of the 316 specimens, 13 strain(4.1%) of Campylobacter jejuni were isolated. Isolation rate for each food was 37.5% in gizzard, 4.1% in Korean sausage and 3.9% in kimpop. Isolated C. jejuni were grouped as biotype I(84.6%), II(7.7%) and IV(7.7%). C. jejuni isolated from street vended foods were resistant to cephalothin(100%), penicillin(84.6%) and erythromycin(76.9%), but sensitive to gentamicin(92.3%), kanamycin(84.7%) and chloramphenicol(77.0%).

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ISOLATION OF Campylobacter jejuni AND C. coli FROM DOMESTIC AND EXPERIMENTAL ANIMALS AND THEIR DRUG SUSCEPTIBILITY

  • Nakai, Y.;Kimura, K.;Sato, M.;Inamoto, T.;Ogimoto, K.
    • 아세아태평양축산학회지
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    • v.7 no.4
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    • pp.505-507
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    • 1994
  • A total of 526 domestic and experimental animals in Miyagi prefecture, Japan were investigated for fecal carriage of Campylobacter jejuni and Campylobacter coli. C. jejuni was detected in chickens (8.2%), dogs (6.3%), pigs (4.3%), cattle (1.8%) and hamsters (1.4%). C. coli was only detected from pigs (20.7%). Drug susceptibility test was performed on 5 strains of C. jejuni isolated from chickens and 13 strains of C. coli isolated from pigs to tylosin (TS), thianphenicol (TP), carbadox (CDX), chroltetracyclin (CTC), vancomycin (VCM), cefoperazone (CPZ), latamoxef (LMOX), GM were highly effective and CTC, CP and PL were moderately effective against both C. jejuni and C. coli. TS and TPH were moderately effective against C. jejuni; however, they were less effective to C. coli. One strain of C. jejuni against CTC considered to be drug resistant. The results suggest that C. jejuni and C. coli can be controlled by several drugs effectively, although a drug resistant strain exists.

Inverse Correlation between Extracellular DNase Activity and Biofilm Formation among Chicken-Derived Campylobacter Strains

  • Jung, Gi Hoon;Lim, Eun Seob;Woo, Min-Ah;Lee, Joo Young;Kim, Joo-Sung;Paik, Hyun-Dong
    • Journal of Microbiology and Biotechnology
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    • v.27 no.11
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    • pp.1942-1951
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    • 2017
  • Campylobacter jejuni and Campylobacter coli are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from chickens, to understand the relationship between extracellular DNase activity and biofilm formation. A biofilm-forming reference strain, C. jejuni NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants. The biofilm non-forming strains or supernatants significantly prohibited the biofilm formation of C. jejuni NCTC11168. In addition, the strains degraded pre-formed biofilms of C. jejuni NCTC11168. Degradation of C. jejuni NCTC11168 biofilm was confirmed after treatment with the supernatant of the biofilm non-forming strain 2-1 by confocal laser scanning microscopy. Quantitative analysis of the biofilm matrix revealed reduction of extracellular DNA (16%) and proteins (8.7%) after treatment. Whereas the biofilm-forming strains C. jejuni Y23-5 and C. coli 34-3 isolated from raw chickens and the C. jejuni NCTC11168 reference strain showed no extracellular DNase activity against their own genomic DNA, most biofilm non-forming strains tested, including C. jejuni 2-1, C. coli 34-1, and C. jejuni 63-1, exhibited obvious extracellular DNase activities against their own or 11168 genomic DNA, except for one biofilm non-former, C. jejuni 22-1. Our results suggest that extracellular DNase activity is a common feature suppressing biofilm formation among biofilm non-forming C. jejuni or C. coli strains of chicken origin.

Campylobacter장염에 의한 식품위생학적인 고찰 (Hygienic Aspects of Campylobacter Enteritis)

  • 이용욱;홍종해
    • 한국식품위생안전성학회지
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    • v.1 no.1
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    • pp.57-66
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    • 1986
  • Campylobacter jejuni has been recognized as one of the causes of human gastroenteritis. The feces of a variety of reservoir animals contain c. jejuni as commensals in the intestinal tracts, and are fundamental source of contamination. The intestinal organisms contaminate carcasses, equipment tools hands of the processing line workers and air of the processing facility. Once the contamination happens in the slaughterhouses or the meat processing facilities, it is very difficult to keep the carcasses free from the infection of c. jejuni. Various disinfectants are effective in minimizing the number of Campylobacter infections in the processing facilities by washing contaminated carcasses, tools, and hands. Direct contact with infected animals has been incriminated in transmission of infection caused by C. jejuni. Freezing, cooling with dry air and gamma irradiation are an effective way for preserving the meat and eliminating the transmission, but broad and enforced studies are needed for the practical use.

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한방사료 첨가제의 항균성 및 재래닭에서의 Campylobacter jejuni 방제효과 (Antimicrobial Activity and Preventive Effect of Oriental Herbal Medicine Feed Additives for Campylobacter jejuni in Korean Native Chickens)

  • 김곤섭;정태성;신기욱;한대용;차혜진;김용환
    • 한국임상수의학회지
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    • v.23 no.1
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    • pp.41-49
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    • 2006
  • In this study, antimicrobial activity of oriental herbal medicine extract (OHME) was tested for some organisms and the preventive effects of OHME for the colonization of Campylobacter jejuni on epithelium of small intestine were examined in Korean native broiler chickens fed a forage added 1.0% OHME. The isolated Campylobacter spp were biotyped, serotyped and the susceptiblility of isolates to antimicrobial agent were examined. The growth of Staphylococcus aureus was inhibited in 0.25% OHME. C. jejuni and C. coli were inhibited in 0.1% OHME, and Salmonella spp, Lactobacillus acidophilus and Escheichia coli 0157 were inhibited in 2.0% OHME. For the application of forage added 1.0% OHME in broiler chicken farm, the frequency of Campylobacter spp from feces, liver and spleen sample of chickens were examined during 2 weeks interval. The frequence of Campylobacter spp in feces from chickens fed assorted forage (control group) was increased from 25% in first week to 75% in seventh week. But the frequence of Campylobacter spp in feces sample from chickens 134 forage added OHME was slightly reduced from 25% in first week to 15% in seventh week. The frequence of Campylobacter spp in liver, and spleen was 13.7% and 10% respectively after seventh week in control group, but the Campylobacter spp was not isolated after fifth week in live and spleen from chickens fed forage added OHME. Isolated 56 strains of thermophilic Campyiobacter from Korean native chickens was classified as C. jejuni (76.7%), C. coli (214%) and C. laridis (1.6%). The majority of 43 isolates of C. jejuni was classified on biotype I (60.4%), II (30.2%). Most of 12 isolates of C.coli were biotype I (83.3%). Isolated 31 strains C. jejuni of showed 11 different serotype, and serotype 36 (18.6%), 17 (13.9%)were most frequent. Isolated 10 strains of C. coli showed 5 different serotypes and serotype 31 (33.3%) and 21 (25%) were relatively common. Isolated Campylobacter spp were highly susceptible to nalidixic acid, amikacin, gentamicin, colistin and chloramphenucol.

도계장 도계의 Campylobacter 균 오염에 관한 연구 (Studies on Campylobacter jejuni and Campylobacter coli contamination on broiler carcasses in slaughterhouse)

  • 나호명;고바라다;박성도;김용환
    • 한국가축위생학회지
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    • v.30 no.1
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    • pp.77-84
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    • 2007
  • The present study was carried out to investigate the incidence of Campylobacter spp. from the chicken carcasses in slaughterhouse. A total of 9 strains were primarily isolated from enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Nine of Gram-negative, catalase-positive and oxidase-positive strains were further isolated by the determination of biochemical characteristics and finally identified as Campylobacter jejuni with HIP 400F and HIP l134R primers. Therefore, this PCR method proved to be useful as a routine diagnostic test for the Campylobacter detection and confirmation of C. jejuni and C. coli in naturally contaminated poultry samples.

계육에 오염된 Campylobacter 균의 불활성화 평가 (Assessment of Inactivation for Campylobacter spp. Attached on Chicken Meat)

  • 장금일;정헌상;김정호;김광엽
    • 한국미생물·생명공학회지
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    • v.33 no.4
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    • pp.302-307
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    • 2005
  • 본 연구에서는 위생제 처리에 의해 계육에 존재하는 Campylobacter 균의 불활성화 효과를 신속하고 직접적으로 평가하고자 하였다 먼저 Campylobacter균의 계육중 오염 부위는 계육 표면의 주름진 틈 사이 및 모공 주변에 존재하였다. 그리고 TSP처리에 의한 Campylobacter균의 불활성화 효과를 in vitro 방법으로 평가한 결과, Campylobacter 균은 활성상태인 나선형에서 구형으로 형태변환이 발생하였는데, 구형으로 변환된 Campylobacter균은 배양된 배지성분이 제거된 경우 불활성화 효과가 나타내는 반면, 배지성분이 잔존한 경우 TSP처리에 의해 불활성화되지 않은 VBNC의 구형 상태로 잔존하였다. 또한 계육 표면에 오염된 Campylobacrer균을 불활성화 시키기 위해 TSP를 처리하였을 때, Campylobacter균의 모양이 구형으로 변환되었지만, TSP처리에 의해 불활성화 효과를 나타내지 않고 계육 표면에 VBNC 형태로 잔존하여 배지성분을 제거시킨 결과와 같은 결과를 나타내었다. 이는 Campylobacter균의 배지성분 내의 유기물 및 계육표면에 존재하는 유기물을 이용하여 TSP에 대한 저항력을 향상시킨 것으로 생각되며, 이는 다양한 위생제 처리에 의한 식품의 안전성을 추구하는데 있어 매우 중요한 문제점이라고 사료된다. 따라서 본 연구에서는 이와 같은 방법을 통해 계육에 존재하는 Campylobacter균에 대하여 위생제 처리에 의한 불활성화 효과를 직접적이면서 신속하게 평가할 수 있는 가능성을 확인하였다.

Campylobacter jejuni에 대한 염소 및 Monochloramine의 살균효과 (Disinfection Effects of Chlorine and Monochloramine on Campylobacter jejuni)

  • 윤만석;오학식;김치경
    • 한국미생물·생명공학회지
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    • v.17 no.6
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    • pp.539-544
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    • 1989
  • 인체에 설사질환을 일으키는 Campylobacter jejuni에 대하여 염소 및 monochlorarmine의 살균효과를 측정하고 세포의 구조형태 및 단백질과 DNA에 대한 영향을 주사전자현미경과 전기영동방법으로 비교 관찰하였다. 0.5mg/l의 염소용액에서 15분간 처리했을 때에는 쉽게 6 log가 사멸되었다. 그리고 염소용액의 pH가 4.5에서 7.0, 10.0으로 높아짐에 따라 C. jejuni에 대한 염소의 살균효과는 점점 떨어졌다. 또한 monochloramine의 경우 0.5와 1.0mg/l에서 60분간 처리했을 경우 각각 2와 7 log의 세균이 사멸되었다. 살균제로 처리된 C. jejuni들은나선간균의 형태가 모두 구형으로 변하면서 세포표면의 파괴와 용균현상이 일어났고 서로 엉켜져 있는 예가 많았다. 그 효과는 사멸효과의 경우에서와 같이 monochloramine보다 염소처리군에서 더 심하였다. 살균제로 처리된 C. jejuni의 단백질과 DNA는 정상세포의 것에 비하여 전기영동상에서 차이가 나타났으며 260nm에서의 흡광도도 감소하였다. 특히 염소처리된 세포의 단백질과 DNA는 처리한 염소농도에 비례하여 큰 차이를 나타냈다.

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