• Title, Summary, Keyword: Campylobacter jejuni

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Experimental infection of Campylobacter jejuni and Campylobacter coli in chicks (유추(幼雛)에 있어서 Campylobacter jejuni 및 Campylobacter coli의 실험적(實驗的) 감염(感染))

  • Chung, Byong-gon;Kang, Ho-jo
    • Korean Journal of Veterinary Research
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    • v.28 no.2
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    • pp.345-353
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    • 1988
  • The present study was carried out to investigate the incidence of C jejuni and C coli in chicken. Also were examined the pathogenicity of the isolates in chick by experimental inoculation. Thermophilic Campylobacter were isolated from 34(45.9%) of the 74 specimens, and classified as 21.6% C jejuni, and 24.3% C coli. In the biotyping of 16 stranis of C jejuni isolates, 37.5% of the strains were grouped as biotype I, 62.5% as biotype II. In the case of 18 strains of C coli isolates, 49.9% of isolates were grouped as biotype I, 55.6% as biotype II. n oral inoculation with $10^4cfu$ of Campylobacter isolates into infant chicks(1 to 3 days-old), 17 days-old and 34 days-old chicks, 32.5% of the chicks developed diarrhea on day 1, 52.5% on day 3, 70.0% on day 5, and 27.5% on day 7, and the peak incidence of diarrhea was reached on day 5. The organisms were found to be discharged in feces one day afterwards. C jejuni and C coli strains were detected from the feces in 87.5% of the chicks on day 5. The organisms were multiplied from $10^4$ to $10^6cfu/gm$ in feces 5 to 7 days after inoculation. C jejuni and C coli recovered from 100% of the cecum, 64.3% of the duodenum, 50.0% of the spleen, 42.9% of the livers, and from 21.4% of gallbladders 7 days after inoculation.

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Inactivation of Listeria monocytogenes and Campylobacter jejuni in Chicken by Aqueous Chlorine Dioxide Treatment

  • Hong, Yun-Hee;Ku, Gyeong-Ju;Kim, Min-Ki;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • v.12 no.4
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    • pp.279-283
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    • 2007
  • Aqueous chlorine dioxide $(ClO_2)$ treatment was used for the inactivation of Listeria monocytogenes and Campylobacter jejuni in chicken. Chicken breasts and legs were inoculated with $8{\sim}9log\;CFU/g$ of Listeria monocytogenes and Campylobacter jejuni, respectively, and then treated with 0, 50, and 100 ppm of $ClO_2$ solution. Aqueous $ClO_2$ treatment decreased the populations of the pathogenic bacteria on the chicken samples. One hundred ppm $ClO_2$ treatment on the chicken breast and leg reduced the populations of Listeria monocytogenes and Campylobacter jejuni by $0.61{\sim}1.93\;and\;0.99{\sim}1.21log\;CFU/g$, respectively. Aqueous $ClO_2$ treatment affected the microbial growth during storage at $4^{\circ}C$ by decreasing the initial microbial populations. These results clearly suggest that aqueous $ClO_2$ treatment should be useful in improving the microbial safety of chicken during storage and extending the shelf life.

Prevalence and Drug Susceptibility of Campylobacter jejuni and Campylobacter coli in Korean Native Goats (한국 재래산양에서 있어서 Campylobacter jejuni 및 Campylobacter coli의 분포와 약제감수성)

  • Kang, Ho-jo;Kim, Yong-hwan;Cho, Hyun-ho
    • Korean Journal of Veterinary Research
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    • v.27 no.2
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    • pp.227-233
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    • 1987
  • This study was conducted to determine the epidemiological characteristics of Campylobacter enteritis. A total of 187 fecal specimens of Korean native goat were examined for the presence of C. jejuni and C. coli by direct plating. Fifty strains isolated were examined for biochemical and serological properties and susceptibility to 19 chemotherapeutic agents. A total of 29(15.5%) C. jejuni and 21 (11.2%) C. coli were isolated from the fecal specimen of 187 Korean native goats. Of the 50 isolates of C. jejuni and C. coli, 29 isolates of C. jejuni grouped as 7 biotypes (1,2,3,4,6,7 and 8) and biotypes 1(34.5%), 2(17.2%) and 3(20.7%) were encountered most frequently. Twenty-one C. coli strains were differentated into biotype I (61.9% of the isolates) and biotype II (38.1%). Of the 29 C. jejuni strains examined, 24(83.0%) were typable by the Lior serotyping scheme and five isolates were non typable. C. jejuni grouped as 8 serotypes, serotype 4(24.1%) and 26(20.7%) were encountered most frequently. In the case of 21 strains of C. coli grouped as 6 serotypes, the most frequent serotypes were 21(28.6%) and 25(23.8%). Total of 50 strains of isolated were all susceptible to amikacin, clindamycin and tobramycine. Overall 85% of isolates were sensitive to erythromycin, doxycycline, chloramphenicol, flume-quine, kanamycin, gentamicin, nalidixic acid, polymyxin B, colistin, tetracycline and ampicillin, but about 65% of isolates were resistant to cefamandole and ethyl hydrocuprein hydrochloride.

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Isolation, Identification, and Characterization of Aero-Adaptive Campylobacter jejuni

  • LEE YOUNG-DUCK;MOON BO-YOUN;CHOI JUNG-PIL;CHANG HAK-GIL;NOH BONG-SOO;PARK JONG-HYUN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.5
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    • pp.992-1000
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    • 2005
  • Campylobacter is one of the emerging foodborne pathogens, and its worldwide incidence rate is extremely high. This study was undertaken to isolate and identify Campylobacter strains from chicken carcasses in the local markets, and analyze their characteristics regarding oxygen tolerance. They were isolated after aerobic enrichment and identified by biochemical, physiological, and morphological characteristics, PCR, and 16S rDNA sequencing. Their oxygen tolerances were analyzed in terms of the cell surface hydrophobicity, cell fatty acid composition, and oxidoreductase. Five strains of C. jejuni were isolated and identified from 61 isolates from 50 chickens. Among them, C. jejuni IC21 grew well in Brucella broth and commercial milk under aerobic condition. However, in the aerobic exposure, the cell surface hydrophobicity of C. jejuni IC21 was almost the same as the other isolates, even though its morphology changed from the spiral-bacilli form into the coccoid form. Fatty acid analyses showed that all Campylobacter strains had a high composition of $C_{19:1}$, cyclopropane fatty acid, and that the amount of the other fatty acids were very similar between them. Interestingly, however, only oxidoreductase activities of C. jejuni IC21 increased highly under aerobic exposure even though its activities were almost the same as the other C. jejuni strains just after microaerobic culture. It had 11.8 times higher catalase activity, 4.4 times higher for SOD, and 2.0 times higher for NADH oxidase activities. Therefore, in the case of the aero-adaptive C. jejuni IC21, expression of oxidoreductase significantly increased under oxidative stressed condition, which might allow it to survive for a longer time and grow on food under aerobic exposure. Such new strain might be one of the explanations for the increase of campylobacteriosis.

A Review on Recently Isolated Campylobacter fetus ss. jejuni (최근 분리된 Campylobacter fetus subsp. jejuni에 관한 소고)

  • Lee, Woong-Yeal;Park, Seung-Hahm
    • The Journal of the Korean Society for Microbiology
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    • v.17 no.1
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    • pp.49-55
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    • 1982
  • Campylobacter fetus subsp. jejuni was isolated from 7 stool specimens(4%) from the total number of 175 stool specimens during February and March, 1982 and the 15 specimens(39.5%) from 33 stool specimens of the chicken in the urban area of Seoul. All isolates showed typical characteristics of the Campylobacter fetus subsp. jejuni in Gram reaction, morphology and biochemical tests. They were all resistant to cephazolin and sensitive to gentamicin and chloramphenicol. During same period of time, the shigella was isolated from 11 stool specimens and the salmonella was isolated from 1 stool specimen.

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Quantitative microbial risk assessment of Campylobacter jejuni in jerky in Korea

  • Ha, Jimyeong;Lee, Heeyoung;Kim, Sejeong;Lee, Jeeyeon;Lee, Soomin;Choi, Yukyung;Oh, Hyemin;Yoon, Yohan
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.2
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    • pp.274-281
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    • 2019
  • Objective: The objective of this study was to estimate the risk of Campylobacter jejuni (C. jejuni) infection from various jerky products in Korea. Methods: For the exposure assessment, the prevalence and predictive models of C. jejuni in the jerky and the temperature and time of the distribution and storage were investigated. In addition, the consumption amounts and frequencies of the products were also investigated. The data for C. jejuni for the prevalence, distribution temperature, distribution time, consumption amount, and consumption frequency were fitted with the @RISK fitting program to obtain appropriate probabilistic distributions. Subsequently, the dose-response models for Campylobacter were researched in the literature. Eventually, the distributions, predictive model, and dose-response model were used to make a simulation model with @RISK to estimate the risk of C. jejuni foodborne illness from the intake of jerky. Results: Among 275 jerky samples, there were no C. jejuni positive samples, and thus, the initial contamination level was statistically predicted with the RiskUniform distribution [RiskUniform (-2, 0.48)]. To describe the changes in the C. jejuni cell counts during distribution and storage, the developed predictive models with the Weibull model (primary model) and polynomial model (secondary model) were utilized. The appropriate probabilistic distribution was the BetaGeneral distribution, and it showed that the average jerky consumption was 51.83 g/d with a frequency of 0.61%. The developed simulation model from this data series and the dose-response model (Beta Poisson model) showed that the risk of C. jejuni foodborne illness per day per person from jerky consumption was $1.56{\times}10^{-12}$. Conclusion: This result suggests that the risk of C. jejuni in jerky could be considered low in Korea.

Ethanol Tolerance of Campylobacter jejuni by Ethanol Shock (Ethanol 충격에 의한 Campylobacter jejuni 의 Ethanol 내성)

  • 김치경;가익현
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.377-382
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    • 1992
  • The responses of C. jejuni to ethanol shock were studied for their survival. synthesis of ethanol shock proteins, and increased survival at higher concentration of ethanol upon prior treatments of ethanol. When C. jejuni were shocked with ethanol at 1. 3. and 5% for 60. 30 and 10 minutes, respectively. those cells synthesized the ethanol shock proteins of 90, 66, 60, 45, and 24 kd in molecular weight. When the C. ,jejuni shocked with 1 and 3% ethanol were exposed to 3 and 5% ethanol for 30 minutes. their survival rates were increased by $10^1$~$10^2$ as compared with those of the cells without ethanol-shock. In the same way. C. ,jejuni shocked with 5% ethanol for 10 minutes :.bowed about 102 times higher survival rates than the cells without ethanol-shock. This result suggests that C jejuni shocked with I-5% ethanol for 10-30 minutes synthesized five kinds of ethanol shock proteins. and that the shock proteins contributed to increase ethanol tolerance for their survival at the higher concentrations of ethanol.

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Prevalence and antimicrobial resistance patterns of Campylobacter jejuni from duck feces (오리 분변에서의 Campylobacter jejuni 오염도와 항생제 내성유형 조사)

  • Kim, Neung-Hee;Chae, Hee-Sun;Kang, Yong-Il;Shin, Bang-Woo;Choi, Nong-Hoon;Kim, Hyo-Bi
    • Korean Journal of Veterinary Service
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    • v.36 no.1
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    • pp.57-60
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    • 2013
  • This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.

Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea

  • Cho, Hyun-Ho;Kim, Sang-Hyun;Min, Wongi;Ku, Bok-Kyung;Kim, Jong-Hyun;Kim, Yong-Hwan
    • Korean Journal of Veterinary Research
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    • v.54 no.2
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    • pp.91-99
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    • 2014
  • An antimicrobial susceptibility test was conducted to compare the resistance rates among Campylobacter spp. isolates from dogs (n = 50) raised under diverse conditions and humans (n = 50). More than 60% of Campylobacter (C.) jejuni from dogs and humans showed resistance to nalidixic acid, enrofloxacin and ciprofloxacin. C. jejuni isolates from humans showed higher resistance to tetracycline (83.3%) and ampicillin (91.3%) than those from dogs. None of the C. jejuni or Campylobacter coli isolates from humans or dogs were resistant to erythromycin. Overall, 85% of Campylobacter spp. isolates showed a multidrug resistant phenotype. Nucleotide sequencing analysis of the gryA gene showed that 100% of $NA^R/CIP^R$ C. jejuni isolates from dogs and humans had the Thr-$86^{th}$-Ile mutation, which is associated with fluoroquinolone resistance. flaA PCR restriction fragment length polymorphism (RFLP) typing to differentiate the isolates below the species level revealed 12 different clusters out of 73 strains. The human isolates belonged to eight different RFLP clusters, while five clusters contained dog and human isolates.

Studies on Epidemiology of Campylobacter fetus subsp. jejuni infection (Campylobacter fetus subsp. jejuni에 관한 역학적 연구)

  • 김신무;정윤섭;이형환
    • Korean Journal of Microbiology
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    • v.21 no.3
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    • pp.170-176
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    • 1983
  • Compylobacter fetus subsp. jejuni infection in Korea has been reported in 1981, but the epidemiological information was lacking at the report. Therefore this study was undertaken to determine the Campylobacter carrier rate of the healthy Korean and of the Korean chicknens, and the antimicrobial susceptibity of the isolates from chicken. 129 stool samples from man and 296 fecal samples from chicken were collected and screened to determine the rates of Campylobacter carrier. Forty-four chicken isolates from Seoul and 30 chicken isolates from Iri city were examined the antimicrobial susceptibility. 1. The carrier rate in were examined the antimicrobial susceptibility 2. The isolation rates of the Campylobacter from Iri chickens were 50.3%. The highest rate was 76.3% on February and July, while the lowest rate ws 20.7% on March. 3. All of chicken isolates from Seoul and Iri were susceptible to amikacin and chromphenicol. 63.3% of the Seoul samples showed resistant to erythromycin.

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