• Title, Summary, Keyword: Campylobacter jejuni

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Chaperon Effects of Campylobacter jejuni groEL Genes Products in Escherichia coli (Campylobacter jejuni의 groEL 유전자 산물의 대장균에서의 Chaperon효과)

  • Lim, Chae-Il;Kim, Chi-Kyung;Lee, Jae-Kil
    • Korean Journal of Microbiology
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    • v.32 no.1
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    • pp.47-52
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    • 1994
  • The cells of Campylobacter jejuni heat-shocked at 48${\circ}C$ for 30 min synthesized the heat shock proteins of HSP90, HSP66 and HSP60. Those heat shock proteins were found to correspond to the heat shock proteins of HSP87, HSP66 (DnaK), and HSP58 (GroEL) of E. coli, respectively. By Southern blot analysis of the chromosomal DNAs of C. jejuni with groESL and dnaK genes of E. coli as DNA probes, the heat shock genes of C. jejuni which are homologous to the E. coli groESL and dnaK genes were found to exist in the chromosomal DNA. The genomic libraries of C. jejuni were constructed with the cosmid vector pWE15 and the groEL gene of C. jejuni were cloned in E. coli B178 groEL44 temperature senstive mutant. The hybrid plasmid (pLC1) was inserted with the DNA fragment (about 5.7kb in size) containing the groEL gene. E. coli groEL44 mutant cell transformed with the pLC1 could grow at 42${\circ}C$ by synthesizing the HSP60 of C. jejuni and regained the susceptibility to the ${\lambda}$ vir phage by expression of the groEL gene in the cloned cells. These indicated that the groEL products of C. jejuni had chaperon effects by synthesizing the heat shock proteins in the cloned cells of E. coli.

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Role of flgA for Flagellar Biosynthesis and Biofilm Formation of Campylobacter jejuni NCTC11168

  • Kim, Joo-Sung;Park, Changwon;Kim, Yun-Ji
    • Journal of Microbiology and Biotechnology
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    • v.25 no.11
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    • pp.1871-1879
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    • 2015
  • The complex roles of flagella in the pathogenesis of Campylobacter jejuni, a major cause of worldwide foodborne diarrheal disease, are important. Compared with the wild-type, an insertional mutation of the flgA gene (cj0769c) demonstrated significant decrease in the biofilm formation of C. jejuni NCTC11168 on major food contact surfaces, such as polystyrene, stainless steel, and borosilicate glass. The flgA mutant was completely devoid of flagella and non-motile whereas the wild-type displayed the full-length flagella and motility. In addition, the biofilm formation of the wild-type was inversely dependent on the viscosity of the media. These results support that flagellar-mediated motility plays a significant role in the biofilm formation of C. jejuni NCTC11168. Moreover, our adhesion assay suggests that it plays an important role during biofilm maturation after initial attachment. Furthermore, C. jejuni NCTC11168 wild-type formed biofilm with a net-like structure of extracellular fiber-like material, but such a structure was significantly reduced in the biofilm of the flgA mutant. It supports that the extracellular fiber-like material may play a significant role in the biofilm formation of C. jejuni. This study demonstrated that flgA is essential for flagellar biosynthesis and motility, and plays a significant role in the biofilm formation of C. jejuni NCTC11168.

Morphology and Adhesion of Campylobacter jejuni to Chicken Skin Under Varying Conditions

  • Jang, Keum-Il;Kim, Min-Gon;Ha, Sang-Do;Kim, Keun-Sung;Lee, Kyu-Ho;Chung, Duck-Hwa;Kim, Cheorl-Ho;Kim, Kwang-Yup
    • Journal of Microbiology and Biotechnology
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    • v.17 no.2
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    • pp.202-206
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    • 2007
  • The adhesion of Campylobacter jejuni to chicken skin, along with the associated morphological changes under aerobic conditions at 4, 25, and $37^{\circ}C$ and microaerobic ($O_2\;5%,\;CO_2\;10%,\;N_2\;85%$) conditions, were investigated using confocal laser scanning microscopy (CLSM), flow cytometry, and plate counting. The morphological change of C. jejuni from a spiral shape to a coccoid form or VBNC form (viable but nonculturable form) progressed rapidly under aerobic conditions at 25, 37, and $4^{\circ}C$. As regards adhesion, the C. jejuni cells were mostly located in the crevices and feather follicles of the chicken skin, where the cells in the feather follicles floated freely in the entrapped water, even after the skin was rinsed quite thoroughly. CLSM also revealed the penetration of some spiral-shaped C. jejuni cells into the chicken skin. Even after changing their shape at various temperatures, coccoid-form C. jejuni cells were still found in the crevices and feather follicles of the chicken skin.

Inactivation of Campylobacter jejuni using Radio-frequency Atmospheric Pressure Plasma on Agar Plates and Chicken Hams

  • Kim, Joo-Sung;Lee, Eun-Jung;Cho, Eun-Ah;Kim, Yun-Ji
    • Food Science of Animal Resources
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    • v.33 no.3
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    • pp.317-324
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    • 2013
  • Radio-frequency driven atmospheric pressure plasma using argon gas was studied in the inactivation of Campylobacter jejuni in order to investigate its applicability. First, the inactivation study was conducted on an agar surface. C. jejuni NCTC11168 was reduced by more than 7 Log CFU after an 88 s treatment. Another strain, ATCC49943, was studied; however, the inactivation was less efficient, with a 5 Log CFU reduction after a 2 min treatment. Then, chicken breast ham was studied at the $10^6$ CFU inoculation level. The inactivation efficiency was much lower for both strains compared to that on the agar plates. C. jejuni NCTC11168 and ATCC49943 were reduced by 3 Log CFU after a 6 min treatment and by 1.5 Log CFU after a 10 min treatment, respectively. The scanning electron microscopy analysis indicated that C. jejuni cells were deformed or transformed into coccoid form under the plasma treatment. During the plasma treatment, the temperature of the samples did not rise above $43^{\circ}C$, suggesting that heat did not contribute to the inactivation. Meanwhile, water activity significantly decreased after a 10 min treatment (p<0.05). This study conveyed that radio-frequency atmospheric pressure plasma can effectively inactivate C. jejuni with strain-specific variation.

Screening of Genes Expressed In Vivo During Interaction Between Chicken and Campylobacter jejuni

  • Hu, Yuanqing;Huang, Jinlin;Jiao, Xin-An
    • Journal of Microbiology and Biotechnology
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    • v.24 no.2
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    • pp.217-224
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    • 2014
  • Chicken are considered as the most important source of human infection by Campylobacter jejuni, which primarily arises from contaminated poultry meats. However, the genes expressed in vivo of the interaction between chicken and C. jejuni have not been screened. In this regard, in vivo-induced antigen technology (IVIAT) was applied to identify expressed genes in vivo during interaction between chicken and C. jejuni, a prevalent foodborne pathogen worldwide. Chicken sera were obtained by inoculating C. jejuni NCTC 11168 into Leghorn chickens through oral and intramuscular administration. Pooled chicken sera, adsorbed against in vitro-grown cultures of C. jejuni, were used to screen the inducible expression library of genomic proteins from sequenced C. jejuni NCTC 11168. Finally, 28 unique genes expressed in vivo were successfully identified after secondary and tertiary screenings with IVIAT. The genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, regulation and other processes, in addition to Cj0092, with unknown function. Several potential virulence-associated genes were found to be expressed in vivo, including chuA, flgS, cheA, rplA, and Cj0190c. We selected four genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results indicated that these selected genes were significantly upregulated in vivo but not in vitro. In short, the expressed genes in vivo may act as potential virulence-associated genes, the protein encoded by which may be meaningful vaccine candidate antigens for campylobacteriosis. IVIAT provides an important and efficient strategy for understanding the interaction mechanisms between Campylobacter and hosts.

Prevalence of Campylobacter jujuni in Broilers and Chicken Processing Plants (육계 및 도계장에서의 Campyobacter jejuni의 오염에 관한 연구)

  • 오정선;신광순;윤용덕;박정문
    • Journal of Food Hygiene and Safety
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    • v.3 no.1
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    • pp.27-36
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    • 1988
  • Generally, carrier chickens contaminate the processing plant equipment to such an extent that negative chickens procell afterwards result in contaminated. meat. This study was performed to investigate the prevalence of Complliobacter jejuni in two chicken procelling plants. Altogether two hundred samples were collected from cloaca, carcasses, chilling water, and evis-cerationknives at different processing stages during the period of June to September 1987. The isolated organisms were tested for distribution of biotype, serotype. The results obtained were summarized as follows: 1. C. jejuni was isolated from 41(34.2%) of 120 chicken feces, 9(45.0%) of 20 carC888eS before chilling, 11(55.0%) of 20 carcasses after chilling, 12(60.0%) of 20 eviscerationlmives. The evilceration knives and chilling water were considered as major means of croll contamination. 2. In biotyping 82 isolates of C. jejuni, 64(78.1%) were cl888ified as biotype I, and 18(21.9%) belonged to biotype II. 3. In serotyping 82 isolates of C. jejuni, 64(78.1%) were identified as serotype LIO 37, and 18(21.9%) were untypable.

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Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

  • Cho, Hyun-Ho;Kim, Sang-Hyun;Min, Wongi;Ku, Bok-Kyung;Kim, Yong-Hwan
    • Korean Journal of Veterinary Research
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    • v.54 no.1
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    • pp.39-48
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    • 2014
  • The prevalence of thermophilic Campylobacter (C.) spp. in stray, breeding, and household dogs was 25.2, 12.0, and 8.8%, respectively. C. jejuni and C. upsaliensis were the predominant Campylobacter spp. from household dogs. cdtA, cdtB, and cdtC were detected by PCR in all isolates. Despite the high cytolethal distending toxin (CDT) gene prevalence, only 26 (31%) C. jejuni strains and one (15.3%) C. coli strain showed evidence of CDT production in HEp-2 cell cytotoxicity assays. Virulence-associated genes detected in the C. jejuni and C. coli isolates were cadF, dnaJ, flaA, racR, ciaB, iamA, pldA, virB11, ceuE, and docC. cadF, dnaJ, flaA, and ceuE were found in all C. jejuni and C. coli isolates. When detecting Guillain-Barr$\acute{e}$ syndrome-associated genes (galE, cgtB, and wlaN), galE was identified in all isolates. However, cgtB and wlaN were more prevalent in C. jejuni isolates from humans than those from dogs. Adherence and invasion abilities of the C. jejuni and C. coli strains were tested in INT-407 cells. A considerable correlation (adjusted $R^2$= 0.678) existed between adherence and invasion activities of the Campylobacter spp. isolates.

Electron Microscopic Studies on the Morphology and Ultrastructures in Campylobacter jejuni treated with Physico-chemical Disinfectants (이화학적 살균제로 처리한 Campylobacter jejuni의 세포 형타와 미세구조에 대한 전자현미경적 연구)

  • Yun, Man-Seok;Oh, Hak-Shik;Kim, Chi-Kyung
    • Applied Microscopy
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    • v.19 no.2
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    • pp.17-26
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    • 1989
  • The cells of Campylobacter jejuni treated with physical or chemical disinfection agents were comparatively examined by scanning and transmission electron microscopies for their morphological features and internal ultrastructures. The normal cells of C. jejuni, showed typical spiral rod shapes. The ribosomes, nucleoids, and other cellular constituents were observed to be distributed evenly throughout the cytoplasm. The cells treated with heat or UV-light were changed to spherical or irregular shapes and their cell envelopes were destroyed to form ghost cells by liberating their cytoplasmic components. The cells treated with chlorine or monochloramine were also changed into irregular round shapes. The chlorinated cells showed very rough surface structures with many blob-like protrusions, while the surface of the monochloramine-treated cells appeared to be relatively smooth.

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Comparison of Multilocus Sequence Typing (MLST) and Repetitive Sequence-Based PCR (rep-PCR) Fingerprinting for Differentiation of Campylobacter jejuni Isolated from Broiler in Chiang Mai, Thailand

  • Patchanee, Prapas;Chokboonmongkol, Chomporn;Zessin, Karl-Hans;Alter, Thomas;Pornaem, Sarinya;Chokesajjawatee, Nipa
    • Journal of Microbiology and Biotechnology
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    • v.22 no.11
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    • pp.1467-1470
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    • 2012
  • We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.

Prevalence and Antimicrobial Susceptibility of Erythromycin-Resistant Campylobacter jejuni and Campylobacter coli Isolated from Swine

  • Choi, Mi-Rai;Kim, Shin-Moo;Kim, Sang-Ha;Choi, Wan-Soo;Kim, Young-Kwon
    • Biomedical Science Letters
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    • v.18 no.2
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    • pp.152-159
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    • 2012
  • Campylobacter species are known to the high optimum growth temperature ($42^{\circ}C$) and the cause of enteritis in people. Erythromycin has a curative effect for enteritis caused by the bacteria. However, the rate of erythromycin-resistant bacteria was not well known until recently in Korea. Swine are one of sources of the infection with a Campylobacter species which cause the symptom of a high temperature. In this study, we cultured rectum fecal specimens of 100 pigs in an area of Buan-gun, Jeonbuk Province during July 2009. As a result, the detection rate of C. jejuni and C. coli and the rate of erythromycin-resistant bacteria for the separated Campylobacter species on the condition of high temperature were investigated. The possession or not of hipO and glyA gene and ciprofloxacin-resistant gene gyrA was also reviewed with biochemical characteristics and PCR.