• Title, Summary, Keyword: Carthami Flos

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Cytotoxicity of Carthami Flos on Human cancer cell-lines(I) (홍화(紅花)가 인체(人體)의 암세포주(癌細胞柱)에 미치는 영향(影響))

  • Han, Jong-Hyun;Yoo, Kwang-Suk;Kang, Sung-Young
    • The Journal of Korean Medicine
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    • v.17 no.2
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    • pp.303-310
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    • 1996
  • The purpose of this study was to investigate effect of water extract of Carthami Flos on the proliferation of human cancer cell-lines. The effects of Carthami Flos on the proliferation of A431, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. The results were as follows; 1. Carthami Flos did not effect A431, HeLa, MOLT-4, K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was increased by the combination of Carthami Flos. 3. Carthami Flos inhibited the proliferation of Balb/c 3T3 cells. 4. Carthami Flos stimulated the proliferation of thymocytes. 5. Carthami Flos stimulated the proliferation of splenocytes. 6. Carthami Flos stimulated the proliferation of human lymphocytes.

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Studies on the antioxidant Effects of Carthami Flos Extract (홍화(紅花) 추출물의 항산화 효과에 대한 연구)

  • Yoo, Jin-Sook;Lim, Hyung-Ho
    • The Journal of Korean Medicine
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    • v.28 no.1
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    • pp.137-147
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    • 2007
  • Objective : The objective of this study was to investigate the antioxidative effects of Carthami Flos extract. Methods : Total antioxidant status was examined by total antioxidant capacity(TAC) and total antioxidant response(TAR) against potent free radical reactions. The effect of Carthami Flos extract was examined far details of total phenolic content concentration at which 1,1-dipheny1-2-picrylhydrazyl(DPPH) radical scavenging activity was inhibited, the inhibitory effect on lipid peroxidation, and the effect on reactive oxygen species(ROS) generation. Results : TAC of Carthami Flos extract at the concentration of 5 mg/ml was 1.84 mM Trolox equivalent. 2. TAR of Carthami Flos extract, on the other hand, couldn't be determined due to interference from unidentified compounds. 3. Total phenolic content of Carthami Flos extract at the concentration of 5 mg/ml was 2.01 mM gallic acid equivalent. 4. Concentration of Carthami Flos extract at which DPPH radical scavenging activity was inhibited by 50% was 6.43 mg/ml as compared to 100% by Pyrogallol solution as a reference. 5. The inhibitory effect of the extract on lipid peroxidation was examined using rat liver mitochondria induced by FeS04/ascorbic acid. Carthami Flos extract at the concentration of 10 ms/ml slightly but significantly decreased TBARS concentration. The extract continued to prevent lipid peroxidation in a dose-dependent manner. 6. The effect of Carthami Flos extract on reactive oxygen species(ROS) generation was examined using a cell-free system induced by hydrogen peroxide/FeS04. Addition of 1 mg/ml of Carthami Flos extract significantly reduced dichlorofluorescein(DCF) fluorescence. Carthami Flos extract caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that the ektract significantly prevented ROS generation in vitro. Conclusion: : Antioxidant efffcts of Carffami ffor extract seem to be due, at least in part, to the prevention offree radical-induced oxidation, fellowed by inhibition of lipid peroxidation.

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Effects of Carthami flos on pacemaker potentials of small intestinal and colonic interstitial Cells of Cajal (홍화의 생쥐 소장 및 대장 카할 간질세포의 향도잡이 전위 조절에 미치는 효능에 관한 연구)

  • Kim, Byung Joo
    • Herbal Formula Science
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    • v.27 no.4
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    • pp.237-244
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    • 2019
  • Objectives : The purpose of this study was to investigate the effects of Carthami flos on pacemaker potentials of small intestinal and colonic Interstitial Cells of Cajal (ICC). Methods : To dissociate the ICC, we used enzymatic digestions from the small intestine and colon in mice. In the ICC, the electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICC. Results : 1. The ICC generated pacemaker potentials in the murine small intestine and colon. 2. Pretreatment with a Ca2+ free solution and thapsigargin, a Ca2+-ATPase inhibitor in the endoplasmic reticulum, stopped the pacemaker potentials. In the case of Ca2+-free solutions, Carthami flos did not induce membrane depolarizations in the murine small intestine and colon. However, when thapsigargin in a bath solution was applied, Carthami flos induced membrane depolarizations only in the murine colon. 3. Pretreatment with 2-APB (transient receptor potential melastatin (TRPM) channel inhibitor) abolished the pacemaker potentials and suppressed Carthami flos-induced effects in the murine small intestine and colon. 4. However, pretreatment with T16Ainh-AO1 (Ca2+ activated Cl- channel; anoctamin 1 (ANO1) inhibitor) did not affect the pacemaker potentials and induced Carthami flos-induced effects only in the murine small intestine. Conclusions : These results suggest that Carthami flos can modulate the pacemaker activity of ICC and the mechanisms underlying pacemaking in ICC might be different in the small intestine and the colon.

The Scavenging Effect on Nitric Oxide in Carthami Flos Herbal-Acupunture Solution (홍화 약침액의 Nitric Oxide에 대한 소거효과)

  • Woo, Dong-Su;Lee, Kyung-Min;Lee, Bong-Hyo;Lim, Seong-Chul;Jung, Tae-Young;Seo, Jung-Chul
    • Journal of Pharmacopuncture
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    • v.10 no.2
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    • pp.25-30
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    • 2007
  • Objectives : Free radical metabolism seems to occupy a remarkably common position in the mechanisms of aging and aging related disease. Oxidative damage to DNA, lipids. proteins and other molecules may contribute to the development of cancer, cardiovascular disease and possibly neurodegenerative disease. This study was designed to find out whether Carthami Flos Herbal-Acupuncture Solution can scavenge Nitric Oxide(NO) or not. SNAP is NO generator. NO concentration was estimated after 2,6, 12 and 24 hrs in no treatment group, after treatment with Vit. C or 1, 10, 100${\mu}$g/m1 of Carthami Flos Herbal-Acupuncture Solution. There was no significant scavenging effect of Carthami Flos Herbal-Acupuncture Solutionon NO after 2 hrs. But there was a significant scavenging effect of Cafhami Flos Herbal-Acupuncture Solution on NO in 10${\mu}$/m1 group after 6hrs. And there was a significant scavenging effect of carthami Flos Herbal-Acupuncture Solution on NO in 1, 10${\mu}$g/ml group after 12, 24 hrs. These results suggest that Carhami Flos Herbal-Acupuncture Solution has scavenging effect on NO. This study shows that Carthami Flos Herbal-Acupuncture Solution can be used for aging related disease and further studies are required to investigate the antioxidative effects of it.

The study of Semen Persicae & Flos Carthami in Bang-Yak-Hap-Pyun (도인(桃仁) 및 홍화(紅花)를 포함하는 방약합편처방(方藥合編處方)에 대한 고찰(考察))

  • Hwang, Sang Joon;Oh, Min seok;Song, Tae won
    • Journal of Haehwa Medicine
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    • v.13 no.1
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    • pp.311-320
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    • 2004
  • In oriental medicine, Semen Persicae & Flos Carthami are frequently used materials for promoting blood circulation to remove blood stasis. Bang-Yak-Hap-Pyun(方藥合編) is widely used for clinical herbal prescription book. In Bang-Yak-Hap-Pyun(方藥合編), Semen Persicae is used 2g~6g. and Flos Carthami is used 1.2g~4g. the nuber of prescription Semen Persicae & Flos Carthami in Bang-Yak-Hap-Pyun is total 17. 2 in sang-tong(上統), 5 in jung-tong(中統), 10 in ha-tong(下統). The group of mostly used disease is about gynecology(婦人科) & five sense organs(五官科). The mostly quoted medical literature what prescription included Semen Persicae & Flos Carthami in Bang-Yak-Hap-Pyun is Dong-Yi-Bo-Gam.

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Effects of Carthami Flos on Human Gastric Cancer Cells (홍화가 인체 위암세포에 미치는 효과)

  • Kim, Jung-A;Han, Song-Ee;Song, Ho-Joon;Chae, Han;Kwon, Young-Kyu;Kim, Byung-Joo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.3
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    • pp.466-470
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    • 2011
  • The purpose of this study was to investigate the anti-cancer effects of Carthami Flos in some kinds of human gastric cancer cells. We used two kinds of human gastric cancer cell lines, such as AGS cells and MKN45 cells. We examined cell death by MTT assay and observed the morphological changes with Carthami Flos. Also, we showed that the combination of sub-optimal doses of Carthami Flos and cisplatin noticeably suppresses in AGS cells and doxorubicin in MKN45 cells. Furthermore, we studied the caspase 3 activity to identify the apoptosis. Therefore, our findings provide insight into unraveling the effects of Carthami Flos in human gastric cancer cells and developing therapeutic agents against gastric cancer.

Effects of Carthami Flos on Human Colorectal Adenocarcinoma Cells (홍화가 인체 대장암세포에 미치는 효과)

  • Han, Song-Ee;Kim, Jung-A;Song, Ho-Joon;Chae, Han;Kwon, Young-Kyu;Kim, Byung-Joo
    • Korean Journal of Oriental Medicine
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    • v.17 no.2
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    • pp.129-134
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    • 2011
  • Objective : The purpose of this study was to investigate the anti-cancer effects of Carthami Flos in some kinds of human colorectal adenocarcinoma cells. Method : We used two kinds of human colorectal adenocarcinoma cell lines, such as HT-29 and WiDr cells. We examined cell death by MTT assay and observed the morphological changes with Carthami Flos. Result : We showed that the combination of sub-optimal doses of Carthami Flos and cisplatin noticeably suppresses in HT-29 cells and doxorubicin in WiDr cells. Furthermore, we studied the caspase 3 activity to identify the apoptosis. Conclusion : Our findings provide insight into unraveling the effects of Carthami Flos in human colorectal adenocarcinoma cells and developing therapeutic agents against colorectal cancer.

Clinical Study on the case of Lumbar compression fracture with Traditional Korean Medicine, Especially the SAAM Acunpture, Carthami-Flos Herbal Acupuncture (사암 어혈방과 홍화약침요법을 병행한 요추압박골절 1례에 관한 고찰)

  • Lee Kil Soong;Lee Geon Mok;Yeom Seong Chul
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.4
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    • pp.1228-1231
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    • 2004
  • There are little report on treatment of compression fracture with Traditional Korean Acupuncture Therapy. We suggest to treat Lumbar compression fracture with the SAAM(사암) Acunpture, Carthami-Flos Herbal Acupuncture Therapy. The Treatment methods of the SAAM(사암). Acnpucture, Eliminating extravasated Blood, was apllied at SP3, LU9, LI11 and Carthami-Flos Herbal Acupuncture at the waist. We treated the patient who was diagnosed as lumbar compression fracture by MRI. After 15 days of Treatment, SAAM Acupuncture and Carthami-Flos Herbal Acupuncture, a remarkable improvement was made for Lumbar compression fracture. There was reports about SAAM Acupuncture and Carthami-Flos Herbal Acupuncture of Patient with Lumbar Compression Fracture. It is very effective to reduce the pain and shortening the period of therapy.

Effect of Corydalidis Tuber.Carthami Flos.Paeoniae Radix Rubra on Anti-Inflamation and function of the Liver at Arthritis Rats (현호색(玄胡索).홍화(紅花).적작약(赤芍藥)이 관절염 흰쥐의 염증과 간(肝)에 미치는 영향)

  • Yoon, Si-Jin;Kim, Youn-Sub
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.2
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    • pp.475-480
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    • 2005
  • This study is designed to find out the effect of Corydalidis Tuber Carthami Flos Paeoniae Radix Rubra mixed water extract on adjuvant arthritis, and to check the stability for Corydalidis Tuber Carthami Flos Paeoniae Radix Rubra mixed water extract about the liver. At first, rats were devided into four groups; Normal(Non-treated group with FCA), Control(Group administrated saline everyday for 1 week after treatment of FCA), Sample A(group administrated Corydalidis Tuber Paeoniae Radix Rubra Carthami Flos mixed water extract $9mg/200{\sim}220g$ everyday 1 week after treatment of FCA) and Sample B(Group administrated Corydalidis Tuber Paeoniae Radix Rubra Carthami Flos mixed water extract $18mg/200{\sim}220g$ everyday 1 week after treatment of FCA). After then, separated searches were made on the change of edema rate, the number of WBC, the contented quantities of total protein, total bilirubin, GOT, GPT in the serum. Result : In the right planter edema, both of Sample groups showed a decrease with statistical significance in comparison with control group. In the WBC count, Sample B group showed a decrease with statistical significance in comparison with control group and also sample A group showed a decrease, but did not show statistical significance. In the serum total protein, both of samples did not show statistically significant decrease in comparison with the control group. In the serum total bilirubin, GOT and GPT, both of samples did not show any harmful change about the liver in comparison with the control group. According to the above results, it is concluded that Corydalidis Tuber Paeoniae Radix Rubra Carthami Flos mixed water extract had the therapeutic effect of anti-inflammation in adjuvant arthritis with no injury to liver.

The Clinical Effects of Carthami-Flos Herbal Acupuncture in Neck Pain Due to Soft Tissue Damage (연부조직(軟部組織) 손상(損傷)으로 인한 경항통(頸項痛) 환자의 홍화(紅花) 약침(藥鍼) 치료(治療)에 관한 임상적 고찰)

  • Kim, Hyo-eun;Kang, Young-hwa;Cho, Meoung-jae;Kim, Tae-woo;Kim, Eun-young;Na, Chang-su
    • Journal of Acupuncture Research
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    • v.19 no.4
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    • pp.112-123
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    • 2002
  • Objective : This study was designed to find out the effects of Carthami-Flos herbal acupuncture theraphy on neck pain due to soft tissue damage. Methods : This study was carried out for 34 patients who had neither structural defectiveness of cervical spine nor neural injury but simple soft tissue damage among poeple who visited Hye-dang Oriental Hospital from December 1, 2001 to June 15, 2002. Group A of 17 patients was taken Carthami-Flos herbal acupuncture treatment. Group B of 17 patients was taken common acpuncture treatment. Results : We have found out the good effects of Cathami-Flos herbal acupuncture on the patients due to soft tissue damage. Conclusion : The Carthami-Flos herbal acupuncture treatment is useful on the patients of neck pain due to soft tissue damage.

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