• Title, Summary, Keyword: Chick Embryo

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TERATOGENICITY STUDY OF SODIUM GLYCYRRHIZINATE IN THE DEVELOPING CHICK EMBRYO

  • Park, Jae-Jun;Lee, Yong-Soon;Ahn, Hee-Yul
    • Toxicological Research
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    • v.5 no.2
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    • pp.167-171
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    • 1989
  • Potential teratogenicity of sodium glycyrrizinate in developing chick embryo was investigated. Body length was shortened significantly by dosage related when compared to untreated and vehicle control, but there was no significant difference on body weight, hind-limb length, claw length in all of the groups.

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Effects of Radioactive Phosphorus (32P) on the Growth of Chick Embryo and Young Chick (방사성(放射性) 인(燐) (32P)이 계태아(鷄胎兒) 및 초생추발육(初生雛發育)에 미치는 영향(影響))

  • Lee, Joon Sup;Yoon, Suk Bong;Ko, Kwang Doo
    • Korean Journal of Veterinary Research
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    • v.11 no.2
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    • pp.117-122
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    • 1971
  • This experiment was performed to study the effects of radioactive phosphorus($^{32}p$) on the growth of chick embryo and young chick. Radioactive phosphorus($^{32}p$) was administered into the yolk sac of chick embryo in doses of 2 uci/gm and 1 uci/gm and was administered intraperitoneally to the young chick in doses of 1 uci/gm and 0.5 uci/gm. The chick embryos were sacrificed on 6th, 7th, 8th, 9th and 10th day and the chicks were sacrificed on 1st, 3rd, 6th and 10th day after the administration and the liver, kidney, spleen, brain, eye ball and femur were weighed to observe the effects of growth inhibition on them. The results obtained were as follows. 1. A marked growth inhibitory effect was found on 8th, 9th and 10th day after the administration of $^{32}p$ in chick embryos and the same effect was found on 6 th and 10 th day after the administration in chicks. 2. The growth of the liver, kidney, spleen and femur was inhibited markedly but the brain and eye ball were not affected in chick embryos and chicks.

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Ultrastructure and Dehydrogenase activity on the Differentiation of the Cerebral Nerve Cell in the Chick Embryo (1) (계배 대뇌의 신경세포 분화에 따른 탈수소효소 활성 및 미세구조 (1))

  • Kim, Saeng-Gon
    • Applied Microscopy
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    • v.28 no.4
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    • pp.563-575
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    • 1998
  • To investigate the changes during the differentiation of the cerebral neurons of chick embryo of tne embryogenic day (ED) 7 and 8, the ultrastructural changes in the cerebral neurons, the activity of dehydronases (LDH, MDH and SDH), protein expression profile and adenosine triphosphate concentration were analyzed. In ED 7 chick embryos, relatively large nucleus, centrally located nucleolus, evenly spread chromatin over nucleoplasm, and prominent nuclear envelope were observed. Oval-shaped mitochondria with well-developed cristae were present over entire cytoplasm. In ED 8 chick embryos, evenly spread chromatin over nucleoplasm, and prominent nuclear envelope were observed. In the cytoplasm, well-developed rough endoplasmic reticulum and Golgi complex were observed. In ED 7 chick embryos and ED 8 chick embryos, 31 polypeptide bands and 34 polypeptide bands were observed, respectively. The activities of dehydrogenases were lower in ED 7 chick embryos than in ED 8 chick embryos. LDH activity was 8.16 (ED 7) and 9.28 (ED 8), MDH activity was 7.98 (ED 7) and 10.10 (ED 8), and SDH activity was 5.49 (ED 7) and 7.14 (ED 8) respectively. The ATP concentration remained unchanged over ED 7 and 8.

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Cell Biological Studies of the Effect of Aromatic Amino Acids on Early Development of Chick Embryo (방향족 아미노산이 초기계배에 미치는 영향에 관한 세포생물학적 연구)

  • 최임순;주충노;최춘근;김재원
    • The Korean Journal of Zoology
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    • v.28 no.4
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    • pp.257-278
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    • 1985
  • The effect of aromatic amino acids such as phenylalaine, tryptophan and tyrosine on somitogenesis at the early stage of chick embryo has been investigated morphologically using light and electron microscopy. Micrographs of aromatic amino acid injected chick embryo showed that an incomplete somite segmentation occurred and some decremental effect on the nervous system were observed. Somites were poorly developed and their size were variable. Electron micrograph of somatic cells from aromatic amino acid injected chick embryo showed that chromatins were coagulated, some of mitochondria were damaged, and nucleus were transformed considerably in some cases. The protein and nucleic acid levels and some enzyme activities of 15-day chick embryo which received the injection of 1mg of aromatic amino acid in 0.05 ml of saline 24 hours after the incubation were analyzed. Protein, DNA and RNA levels of the test group were not lowered significantly but the activities of enzymes for basic metabolism, such as lactate dehydrogenase, succinate dehydrogenase, malate dehydrogenase and glucose 6-phosphate dehydrogenase were considerably lowered as compared with those of control. From the present expeerimental results, it was tentatively suggested that the administration of amino acid might slow down the yolk granule degradation probably by feed back mechanism resulting in the disturbance of amino acid balance in the cell, which might give rise to impair normal metabolic pattern leading to abnormal somitogenesis to chick embryo at very early stage of development.

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Studies on the Teratogenicity of Food Additives in the Developing Chick Embryo (Chick Embryo를 이용한 식품첨가물의 독성에 관한 연구)

  • 최재준;이영순;안희열
    • Journal of Food Hygiene and Safety
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    • v.4 no.3
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    • pp.185-190
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    • 1989
  • 1. Srobic Acid 투여군은 무처치대조군에 비하여 embryo의 체장과 체중이 용량의존적으로 증가를 나타내었는데 체장에 있어서는 1.0ml/egg 및 5.0ml/egg 투여군이, 체중에 있어서는 0.5ml/egg 투여군에서 유의성이 있었다. 2. 2.5ml/egg 수준으로 BHT를 투여한 실험군은 무처치대조군 및 용매대조군에 비하여 체중, 체장, 전지 및 후지에 있어서 유의성 있는 감소를 나타내었다. 3. Sorbic acid 투여군의 기형발생은 뚜렷한 것은 나타나지 않았으나 1.0ml/egg 투여군에서는 hydrocephalus를 가지고 있는 embryo를 볼수 있었다. 4.BHT 투여군의 기형발생률은 무처치 대조군에 비하여 유의성은 없었으나 전지이상 , 혈종 및 hyrocephalus 가 나타났다.

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Congenital Malformation Caused by Bisphenol A in Developing Chick Embryo

  • Kim Su Won;Kim Jin Sik;Ryu Hye Myung;Nam Jin Sik;Cheigh Hong Sik;Min Byung Tae;Park Soo Hyun;Yoo Min
    • Biomedical Science Letters
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    • v.10 no.4
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    • pp.397-401
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    • 2004
  • We have examined congenital malformation in developing chick embryo caused by endocrine disruptor, bisphenol A (BPA). We injected BPA into the air sac of developing egg on day 4 of incubation. BPA-treated group with a concentration of 10 ㎍/egg showed a little decrease on their body length compared to the untreated group. But the group treated with 50㎍/egg revealed severe malformation in eyeballs and bills. The group treated with 100㎍/egg could not continue their development after few days of incubation. These results indicate that BPA clearly inhibits the normal development in chick and it should be toxic to the developing fetus at early stage and in various tissues. The study should contribute to the understanding of toxic effect of BPA in developing human fetus when exposed to the BPA.

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Studies on the Toxicity of Alcohol in the Developing Chick Embryo

  • Kim Jin Sik;Kim Su Won;Ryu Hye Myung;Nam Jin Sik;Min Byung Tae;Park Soo Hyun;Jeon Jung Tae;Yoo Min
    • Biomedical Science Letters
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    • v.10 no.3
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    • pp.305-308
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    • 2004
  • We have examined alcohol-induced malformation of chick embryo. Alcohol was considered to induce the malformation of developing embryo and to have bad effects on embryonic stage. We injected alcohol into air sac on day 4 of incubation. Ten % alcohol-treated group showed a little decrease on their body length compared to the untreated group and distilled water-treated group. Thirty % alcohol-treated group showed significant decrease on their body length compared to the untreated group and 10% ethanol treated group. In addition, we have observed malformation of eyeballs and bills. These results indicate that alcohol affects chicken developments and brings on malformation of developing stage.

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Cell Biological Studies of the EfEect of Serotonin on Chick Embryogenesis (초기계배 발생에 미치는 Serotonin의 영향에 관한 세포생물학적 연구)

  • 최임순;주충노
    • The Korean Journal of Zoology
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    • v.30 no.4
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    • pp.432-444
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    • 1987
  • Very early stage of chick embryo cultivated in the medium containing DLtryptophan by beaker method has been investigated in vitro morphologically using electron microscopy at cellular level and found that the development of tryptophan treated chick embryos corresponding to 18∼66 hrs incubation was impaired and york granule degradation was significantly delayed. It was also found that DNA, RNA and protein biosynthesis of tryptophan treated chick embryo was greatly lowered than those of control group. Conversion of L-tryptophan into serotonin was traced using 14C-L-tryptophan and found that 13.8cA of added radioactivity was recovered from serotonin formed during 18 hrs incubation and the amounts of serotonin formed were depend upon added amount of tryptophan in e99 yolk. It seemed that the serotonin formed from external tryptophan might inhibit the degradation of yolk granule by feedback mechanism, resulting in malformation of chick embryogenesis.

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