• Title, Summary, Keyword: Clostridium perfringens

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Detection of Escherichia coli(K99), Clostridium perfringens and Cryptosporidium parvum in Diarrhetic Feces of Korean Native Calves (한우 송아지의 설사 분변에서 Escherichia coli(K99), Clostridium perfringens와 Cryptosporidium parvum의 검출)

  • Chon, Seung-Ki;Lee, Han-Kyung;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.24 no.4
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    • pp.588-592
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    • 2007
  • The prevalence of E. coli(K99), Clostridium perfringens and Cryptosporidium parvum on acute diarrhea in suckling Korean native calves was evaluated in the field by a veterinary practice. In diagnosis, fecal samples were directly collected from calves that had diarrhea between 2 and 98 days of age. 40 samples were analyzed in October, 2006 and December, 2006. Clostridium perfringens and Cryptosporidium parvum were detected in 15(37.5%) and 4(10.0%) of the samples from diarrhetic calves, respectively. However, E. coli(K99) was not detected in the samples from diarrhetic calves. There was no significant difference(p>0.05) between October(5, 25.0%) and December(10, 50.0%) in incidence of detected Clostridium perfringens from diarrhetic calves. On the other hand, significant differences (p<0.05) in the detection rate of Clostridium perfringens were found between the within 1 month age and all other age groups. In the detection of Cryptosporidium parvum, there was no significant difference(p<0.05) between October (2, 10.0%) and December(2, 10.0%) in the incidence of detected Cryptosporidium parvum from diarrhetic calves. These results suggest that causative agents of calf diarrhea occurred frequently with Clostridium perfringens infection than E. coli(K99) and Cryptosporidium parvum.

Necrotic Enteritis Caused by Clostridium perfringens in California sea lion (Zalophus californianus) (바다사자에서 Clostridium perfringens 감염에 의한 괴사성 장염증례 보고)

  • 신남식;권수완;이기환;김양범;최재훈;박주연;유한상;김대용;권오경
    • Journal of Veterinary Clinics
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    • v.16 no.1
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    • pp.218-221
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    • 1999
  • A case of enetritis caused by Clostridium perfringens was observed in 4years old, male California sea lion(Zalophus californianus). Clinical signs were included in anorexia, depression, diarrhea and vomitting, and the animal died on 4 days after showing clinical signs. Grossly, bloody contents were presented in the intestine and the intestinal mucosa were congested and dark redcolored, and also there were serous fluid in the peritoneal cavity. Histopathologically coagulative necrosis destroyed two third of the villus. Clostridium perfringens were isolated from the intestinal contents. Based on the gross and histopathological findings, this case was diagnosed as necrotic enteritis caused by Clostridium perfringens infection.

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Antibiotic Sensitivity of Clostridium perfringens Isolated from feces of Siberian Tiger with Chronic Diarrhea (시베리안 호랑이의 만성설사 분변에서 분리한 Clostriduium perfringens의 항생제 감수성)

  • 나기정;이완규;양만표
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.475-479
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    • 2000
  • The purpose of this study was to investigate the cause of chronic diarrhea from anaerobic bacteria isolated from Siberian tiger with chronic diarrhea. Major anaerobic bacteria isolated from faces were Clostridium perfringens and their population was $6 {\times} 10^3$ cfu/g feces. Antibiotic sensi- tivity test against Clostridium perfringens was performed using 6 antibiotic drugs including colistin gentamicin, trimethoprim/sulfamethoxazole, amikacin, enofloxacin and penicillin. Clostridium per- fringens were sensitive to amikacin, enrofloxacin and penicillin while they were resistant to colistin. gentamicin and trmetboprim/sulfamethoxazole. And we found that chronic diarrhea of Siberian tiger was treated successfully with penicillin. These results suggested that Clostridium perfringens may bee a cause of chronic diarrhea in Siberian tiger.

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Studies on the Clostridium perfringens type C infection of pig in Korea (국내(國內) 돼지의 Clostridium perfringens type C 감염증에 관한 연구)

  • Yeh, Jae-gil;Park, Kyoung-yoon;Cho, Soung-kun
    • Korean Journal of Veterinary Research
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    • v.33 no.3
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    • pp.419-427
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    • 1993
  • Thirteen strains of Clostridium perfringens were isolated from the pigs with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxin and susceptibility to antimicrobial agents of the isolates were investigated. The results could be summarized as follows ; 1. Almost of the pigs affected with hemorrhagic enteritis, 17 cases examined from 1989 to 1992, were piglets less than 7 day old. 2. The average mortality rate of piglet less than 7 day old affected with hemorragic and necrotic enteritis was 48.5%. 3. The clinical signs of pigs with hemorrhagic enteritis were depression, hemorrhagic diarrhea, anemia and dehydration. Necropsy of the infected pigs showed typical hemorrhage of upper intestine and necrosis of mucosal membrane. 4. The characteristic biochemical properties of the isolates were 2-band hemolysis, positive reaction of reverse CAMP test and formation of LV precipitate in egg yolk medium. 5. The toxin type of the 13 isolates, investigated by mouse inoculation test, was all type C strains of Clostridium perfringens. 6. In susceptibility test to antimicrobial agents, 13 isolates of Clostridium perfringens were highly sensitive to ampicillin, enrofloxacin(Baytril), cephalothin, penicillin and trimethoprim-sulfamethoxazole.

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The change of the population of Clostridium perfringens isolated from intestinal contents in slaughter cattle (도축우 소장에서 Clostridium perfringens 분리 및 시간경과에 따른 균수변화 추이)

  • 김정화;최일영;홍현표;조민희;박영구
    • Korean Journal of Veterinary Service
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    • v.22 no.2
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    • pp.151-158
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    • 1999
  • This study was conducted to investigate the biochemical characteristics, the population and antibiotic susceptibility test of Clostridium perfringens isolated from intestinal contents of slaughter cattle in Kyung-ju and Po-hang. 1. In slaughter cattle Cl perfringens were isolated from intestinal contents of 51 of 101 cases(50.4%) and the population were $\leq$$10^5$cfu/ml of 44 cases(86.3%). 2. In antibiotic susceptibility test, ampicillin, bacitracin, cephalothin, penicillin polymyxin B were highly susceptible, ohloramphenicol, erythromycin, tetracycline were lowly susceptible, gentamicin, kanamycin, amikacin, streptomycin, sulfamethoxine, sulfamethazine were resistant. 3. In leaving test intestinal contents leaved for 0, 4, 8, 16, 32 hours in room temperature and population of Cl perfringens were gradually increased.

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Antibiotic Susceptibility of Clostridium perfringens Isolates from Healthy Sheep and Dogs (건강한 산양 및 개에서 분리한 Clostridium perfringens의 항생제에 대한 감수성)

  • 정희곤
    • The Korean Journal of Food And Nutrition
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    • v.11 no.3
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    • pp.289-292
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    • 1998
  • Identification and antibiotic susceptibility of Clostridium perfringens isolates from fecal specimens of healthy sheep and dogs were performed from December, 1995 to November, 1996 in Kwang-ju and Chonnam area. C. perfringens was isolated in 3 strains(15.0%) out of 20 healthy sheep and 2 strains(6.7%) out of 30 healthy dogs. In antibiotic susceptibility test of C. perfringens, 80% of the isolates was susceptible to ampicillin, baytril, and penicillin, 60% to cephalothin, and 40% to erythromycin.

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Optimal Extracting Condition of Growth-inhibitory Component of Wormwood (Artemisia princeps) against Clostridium perfringens (쑥의 Clostridium perfringens 생육 저해 물질의 최적 추출조건)

  • Park, Jong-Hyun;Kwon, Min;Yoo, Jin-Young;Koo, Young-Jo;Kwon, Dong-Jin
    • Applied Biological Chemistry
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    • v.40 no.4
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    • pp.267-270
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    • 1997
  • To industrialize the foods using wormwood extract, the optimal extraction yield of growth-inhibitory component of wormwood (Artemisia princeps) against Clostridium perfringens in vitro was investigated at various conditions. The optimal extraction of wormwood was produced from the condition of $40^{\circ}C$, 4 hours and 100% ethanol (30 volume).

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Mass mortality of Clostridium perfringens infection in Hanwoo (Clostridium perfringens 감염에 의한 한우 집단 폐사 증례)

  • Lee, Joung-Won;Kim, Ji-Young;Kwak, Kil-Han;Yuk, Hyun-Su;Shon, Ku-Rye;Koh, Won-Seuk
    • Korean Journal of Veterinary Service
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    • v.34 no.1
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    • pp.31-35
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    • 2011
  • This is a case report that 24 heads of cattle suddenly died without clinical signs in a Hanwoo farm. The cause of death was Clostridium perfringens enterotoxemia resulted from them with feeding leftover food. The clinical signs were observed just before the death; increase of heart rate, shallow and rapid respiration, amyostasia, spasm and so forth. In autopsy, blood coagulation disorder, a little abdominal inflation, hepatomegaly and different size of red spots, congestion hemorrhage and undercurrent of bloody exudation were observed in the entire parts of small and large intestines. C. perfringens were isolated from the substantive organs, and a unique fragment of 405bp C. perfringens was amplified by PCR. Therefore, this case was diagnosed as enterotoxemia caused by ${\alpha}$-toxin of C. perfringens A type.

PCR-Based Detection and Molecular Genotyping of Enterotoxigenic Clostridium perfringens Isolates from Swine Diarrhea in Korea

  • Kim Sang-Bum;Lim Hyeong-Jun;Lee Wan-Kyu;Hwang In-Gyun;Woo Gun-Jo;Ryu Sang-Ryeol
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.291-294
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    • 2006
  • Clostridium perfringens strains were isolated from swine diarrhea in Korea. Three out of nineteen (15.8%) isolates of C. perfringens were found to be enterotoxigenic by PCR analysis. PCR-based genotyping of the three enterotoxigenic isolates of C. perfringens revealed that they were types A, C and D, respectively. These results suggest that various types of enterotoxigenic C. perfringens can cause swine diarrhea, and that the presence of enterotoxigenic type A strain, known to be strongly associated with food poisoning, may cause public health problem in Korea.

Detection of Clostridium perfringens and its toxinotypes by enzyme linked immunosorbent assay from enterotoxaemic goats in Bangladesh

  • Islam, K.B.M.S.;Rahman, M.S.;Ershaduzzaman, Md.;Taimur, M.J.F.A.;Jang, Hyung-Kwan;Song, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.37-44
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    • 2010
  • An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.