• Title, Summary, Keyword: Coix lachryma-jobi L. var. ma-yuen Stapf

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Effect of the Seeds of Coix Lachryma-Jobi L. var. Ma-yuen Stapf. on the Proliferation of L1210 cells, a Leukemic cell-lines (율무가 백혈병세포주인 L1210 세포의 증식에 미치는 영향)

  • Lee, Dong-Hee;Jeon, Yong-Geun;Eun, Jae-Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.3
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    • pp.705-709
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    • 2005
  • The purpose of this research was to investigate the effects of Coix Lachryma-Jobi L. var. Ma-yuen Stapf. on the proliferation of L1210 cells and immune cells. The hexane fraction of Coix Lachryma-Jobi decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. Also, the fraction decreased the cell viability of murine thymocytes and splenocytes, but increased the phagocytic activity of murine peritoneal macrophages. In addition, fatty acids and fatty acid methyl esters decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. The main components of the fraction are fatty acid and their derivatives. These results indicate that Coix Lachryma-Jobi has a cytotoxicity on tumor cells and immune cells.

Electrophoretic Characterization of Job′s tears (Yulmoo: Coix lachryma-jobi L. var. Ma-yuen stapf. & Yeomjoo: Coix lachryma-jobi L.) proteins (율무와 염주 단백질의 전기영동 특성)

  • 우자원
    • Korean journal of food and cookery science
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    • v.7 no.3
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    • pp.13-20
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    • 1991
  • This study was performed to examine the electrophoretic properties of Job's tears (Yulmoo: Coix lachryma-jobi L. var. Ma-yuen Stapf. & Yeomjoo: Coix lachryma-jobi L.) proteins. Albumins, globulins, gliadins and glutelins were extracted from the polished Yulmoo and brown Yeomjoo by the modified Osborne method. For a comparison, rice proteins were extracted and fractionated by the same method. The relative proportions of protein fractions were 17.4 : 19.6 : 55.2 : 7.7% in polished Yulmoo, 12.6 : 62.2 : 4.2 :21.0% in brown Yeomjoo and 14.2 : 57 4 : 0.77 : 27.8% in rice, in the order of albumis, globulins, gliadins and glutelins. Polyacrylamide gel electrophoresis (PAGE) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) were peformed to identify the subfractions of each protein fraction extracted from polished Yulmoo, brown Yeomjoo and rice. The electro-phoregrams of polyacrylamide gel electrophoresis showed that the same fractions of both polished Yulmoo protein and brown Yeomjoo protein had very similar electrophoretic patterns to each other respectively, but there were significant differences in the patterns between Job's tears proteins and rice proteins.

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Tyrosinase-inhibitory and Radical Scavenging Activities from the Seeds of Coix lachryma-jobi L. var. ma-yuen [Roman] Stapf (율무의 tyrosinase 저해활성과 radical 소거효과)

  • Kim, Jong-Kil;Lee, Heum-Sook
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1409-1413
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    • 2000
  • The seeds of Coix lachryma-jobi L. var. ma-yuen(yullmoo) were investigated for the free radical scavenging and mushroom tyrosinase inhibitory activities. Brown yullmoo, which contains bran in the seed, has also been evaluated for comparison. After partitioning of the total methanol extracts of yullmoo and brown yullmoo, each organic layer exhibited mild or potent dose-dependent inhibition(about 20-80%) on 1,1-diphenyl picryl hydrazyl(DPPH) free radical with the concentration of $300\;{\mu}g/ml$ to $800\;{\mu}g/ml$. n-Butanol fraction of yullmoo showed the most potent scavenging effect on DPPH free radical. $IC_{50}$ of n-butanol fraction was $423\;{\mu}g/ml$, about six to seven fold higher concentration than standard BHT at the same inhibition rate. As for the tyrosinase inhibitory activity, all of the fractions including the methanol extract exhibited the similar activities even after partitioning. The chloroform fraction of brown yullmoo was found to show the most potent inhibitory activity with an $IC_{50}$ of $321.5\;{\mu}g/ml$.

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Binding of Calcium and Iron by Job′s tears (Yulmoo: Coix lachryma-jobi L. var. Ma-yuen Stapf.) Total Dietary Fiber and Acid Detergent Fiber (율무 식이섬유의 칼슘, 철분과의 결합특성)

  • 우자원
    • Korean journal of food and cookery science
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    • v.7 no.4
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    • pp.87-91
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    • 1991
  • This study was performed to examine the mineral binding properties of total dietary fiber (TDF) and acid detergent fiber (ADF) from Job's tears (Yulmoo: Coix lachryma-jobi L. var. Ma-yuen Stapf.) bran. The endogenous Ca and Fe contents of dietary fiber extracted from Yulmoo bran were measured by atomic absorption spectrophotometer (AAS). Ca contents were 40, 118.6$\mu\textrm{g}$ Ca/g TDF and 131.6 $\mu\textrm{g}$ Ca/g ADF. Fe contents were 1,219.7 $\mu\textrm{g}$ Fe/g TDF and 277.5 $\mu\textrm{g}$ Fe/g ADF. The calcium binding capacities of dietary fiber at pH 5.0, 6.0, 7.0 were 50.77, 77.57, 86.64% for TDF, 40.27, 51.42, 40.90% for ADF, respectively. The iron binding capacity of dietary fiber at pH 5.0~7.0 was 95~97% for TDF and 85~95% for ADF.

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Phytochemical constituents of Coix lachryma-jobi var. ma-yuen roots and their tyrosinase inhibitory activity (율무근의 식물화학적 성분 연구 및 Tyrosinase 저해 활성)

  • Choi, Yun-Hyeok;Choi, Chun Whan;Lee, Jae Yeon;Ahn, Eun-Kyung;Oh, Joa Sub;Hong, Seong Su
    • Journal of Applied Biological Chemistry
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    • v.60 no.1
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    • pp.49-54
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    • 2017
  • In the course of screening tyrosinase inhibitory activity, EtOAc-soluble fraction of Coix lachryma-jobi var. mayuen Stapf. (Gramineae) roots showed significant inhibition. Further fractionation of the EtOAc-soluble fraction resulted in six compounds, which were identified as (+)-icariol $A_2$ (1), zhepiresionol (2), 4-hydroxybenzaldehyde (3), trans-${\rho}$-coumaric acid (4), N-(2-hydroxy-4-methoxyphenyl)-2-hydroxyacetamide (5), and coixol (6). The chemical structures of these compounds were identified on the basis of spectroscopic methods (MS, 1D and 2D NMR) and comparison with literature values. Compound 1 was first reported from this plant. Also, this is the first time that the isolation of compound 5 has been reported from nature source. Among the isolated compounds, compounds 4 and 6 showed enzyme inhibitory activity, with $IC_{50}$ values of 6.5 and $62.4{\mu}M$, respectively, in comparison with these of positive control, arbutin.

Cytotoxicity and Multidrug -Resistance Reversing Activity of Extracts from Gamma-Irradiated Coix Zachryma-jobi L. var. ma-yuen Stapf Seed (감마선 조사된 율무종자의 세포독성 및 다제내성 극복활성)

  • Cha, Young-Ju;Lee, Sook-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.5
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    • pp.613-618
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    • 2005
  • This study was carried out to examine the effects of gamma irradiation on the cytotoxicity and multidrug-resistance reversing activity of methanol extracts from Coix lachryma-jobi L. var. me-yuen Stapf seed. The seed was irradiated with doses of 1, 4, 8, 16, 32 and 64 Gy of the gamma radiation, and then extracted by methanol. The extracts were examined for cytotoxicity on the human cancer cell lines, MCF-7 (human breast adenocarcinoma pleural effusion), Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells, and investigated for multidrug-resistance reversing activity using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. The growth inhibitory activity of irradiated seed extracts on human cancer cell lines was higher than that of the control. In the case of Calu-6 cell line, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. $IC_{50}$ value in the MCF-7 cell line was measured in the only 8 Gy extract. And in the SNU-601 cell line as Calu-6, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. But the extracts of gamma-irradiated seed over 32 Gy showed little growth inhibitory effect against human cancer cell lines. In this result, 8 Gy extract had significant growth inhibitory in all human cancer cell lines $(Calu-6:\;633\;{\mu}g/mL,\;MCF-7:\;653\;{\mu}g/mL\;and\;SNU-601:\;683\;{\mu}g/mL)$. The extracts of 4, 8 and 16 Gy strongly potentiated vincristine cytotoxicity in AML-2/D100 cells. The reversal fold (RF) of 4, 8 and 16 Gy extracts was 1.7, 1.8 and 1.6, respectively. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. These results indicate that the above samples would contain some principles which have cytotoxicity and multidrug-resistance reversing activity. Irradiation technology can be applied to promote physiological activities of medicinal plant seeds.

Analysis of Volatile Components in Korean Rice Wine by the Addition of Yulmoo (탁주 제조 시 율무 첨가에 따른 휘발성 성분의 분석)

  • 신순영;서수환;조원대;이효구;황한준
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1206-1213
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    • 2003
  • In order to use nutraceutical components in Yulmoo (Coix lachryma-jobi L. var. ma-yuen Stapf), the acceptability of Yulmoo for Takju (Korean rice wine) was examined in terms of the production of volatile components. When Yulmoo was added to the Takju made by commercial koji as a substitute of starch at 0 (rice 100%), 15, 30, 60, and 100%, the ethanol content slightly increased to 13.6, 15.2, 15.2, 14.1, and 13.8%, the Production of isobutanol and iso-imyl alcohol increased as the amount of Yulmoo increased. While the use of Yulmoo to the Takju as the nuruk (Korean traditional koji), inoculated with Aspergillus kawachii (AK) and Rhizopu japonicus (RJ), decreased the contents of n-propanol, iso-butanol, and iso-amyl alcohol as well as the contents of ethanol from 14.3% to 11.2 [AK], 7.5 [RJ], and 10.4% [AK + RJ]. The Yulmoo nuruk in Takju increased the production of acetaldehyde and ethyl acetate indicating the negative effect in the organoleptic evaluation. It was determined that the addition of Yulmoo as a starch source or as nuruk has a critical effect on the production of fusel oils and other volatile compounds in Takju fermentation.

Effects of Sowing Times and Spacing on Growth and Yield of Coix lachryma-jobi L. var. ma-yuen STAPF (율무의 파종기(播種期)와 재식밀도(栽植密度)에 따른 생육(生育) 및 수량(收量))

  • Yi, Eun-Sub;Lee, Jun-Seok;Lee, Hyo-Sung
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.3
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    • pp.225-231
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    • 1997
  • This study was conducted to evaluate the growth and yield by sowing times and spacing using machine seeder on Coix Lachryma-jobi L. var ma-yuen $S_{TAPF}$. Adlay was seeded at four different dates (April 20, May 5, May 20 and June 5). Planting spaces were controlled by the seed roller of tractor drill seeder attached to tractor at three spacing ($60{\times}15cm,\;70{\times}15cm\;and\;80{\times}15cm$). The results were summarized as follows. Required days to emergence and days to anthesis were shortened as sowing date was late. But days to maturity were prolonged when sowing date was late. The accumulated temperature increased such as required periods increased. In growth characteristics, culm length was significantly different at different sowing times. But all growth characteristics was not affected by different spacings. In yield components, 1,000 grain weight and ripening rate were significantly different at different sowing times, also the number of tillers and branches was significantly affected at different spacings. Grain yield/ha was significantly different at different sowing times. But it was not significantly different at different spacings. Thus, in order to improve the yield of adlay using drill seeder, if it is not frost, sowing should be done as early as possible. As a result, suitable sowing time was April 20 with spacing at $60{\times}15cm$ using machine seeder in Korea.

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Functional Characteristics of Job's-tears Flakes Subjected to Varying Degree of Heating (율무 flake 제조시 가열정도에 따른 특성)

  • Lee, Young-Tack;Seog, Ho-Moon;Kim, Sung-Soo;Hong, Hee-Do;Kim, Kyung-Tack
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.640-645
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    • 1995
  • Job's-tears(Coix lachryma-jobi L. var. Ma-yuen Stapf) seeds were processed to flakes by soaking, heat treatment, intermediate-moisture drying, flaking, drying, and cooking. Some functional properties of job's-tears flakes subjected to varing degree of heating were characterized by specific volume, texture, water absorption index(WAI), water solubility index(WSI), and viscosity. As degree of gelitinization increased within the intermediate range of $60{\sim}70%$, steam-cooked Job's-tears demonstrated appropriate rheological properties for further processes, resulting in increased specific volume and decreased breaking strength. However, excessive heat treatment rendered Job's-tears undesirably sticky for flaking, which caused adverse effects on the functional properties of flakes such as specific volume and breaking strength. Increasing steaming time$(5{\sim}30\;min)$ caused an increase in WAI and WSI values. Apparent viscosity of powdered flakes was increased with the extent of gelitinization as a function of heat processing. Viscosity of powdered flakes determined using the amylograph increased with the degree of heating applied to Job's-tears at low pasting temperatures, accompanied by a decrease in maximum viscosity as the pasting temperature increased.

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