• Title, Summary, Keyword: Confocal

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Solid-immersion lens based confocal microscopy using super-continuum generation effect (Super-continuum generation 현상을 이용한 Solid-immersion lens 기반 공초점 현미경)

  • Lee, Won-Sup;Moon, Hyungbae;Lim, Geon;Choi, Guk-Jong;Park, No-Cheol
    • Transactions of the Society of Information Storage Systems
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    • v.11 no.2
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    • pp.22-25
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    • 2015
  • In this paper, we demonstrate solid-immersion lens based confocal microscopy using super-continuum generation effect. Using super-continuum generation effect, we could diversify the excitation wavelength of confocal microscopy. Further, high refractive index of solid-immersion lens would increase the resolution of confocal microscopy. As a result, by applying the super-continuum generation effect and solid-immersion lens to confocal microscopy, some problems of confocal fluorescent microscopy, the excitation wavelength and the resolution, could be overcome. To verify it, we made home-built solid-immersion lens based confocal microscopy using super-continuum generation effect, and evaluate the performance of the system.

Signal increasing method in confocal scanning microscopy in fluorescence mode using curved mirror

  • Kang, Dong-kyun;Seo, Jung-woo;Gweon, Dae-gab
    • 제어로봇시스템학회:학술대회논문집
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    • pp.99.3-99
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    • 2001
  • In fluorescence mode confocal scanning microscope, level of detected signal is very low. In object scanning type confocal scanning microscope, the additional optical system with objective lens and plane mirror was proposed to increase signal intensity, but there was none for beam scanning type confocal scanning microscope. We propose reflecting optical systems which improve signal intensity in beam scanning type confocal scanning microscope. We choose one of the proposed optical systems and design the optical system, i.e., select optical components and assign distances between the selected components. To design the optical system, we use finite ray tracing method and make cost function to be minimized.

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Flow Visualization in Microchannel Using Confocal Scanning Microscope (공초점 주사현미경을 통한 미세 유로에서의 유동 가시화)

  • Chang Jun Keun;Park Sung-Jin;Kim Jung Kyung;Han Dong Chul
    • Journal of the Korean Society of Visualization
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    • v.1 no.1
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    • pp.28-33
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    • 2003
  • This paper presents the visualization method in which 3-dimensional(3D) microchannel flow can be detected using a confocal scanning microscope. By soft-lithography, we fabricated various Bio-MEMS(Micro Electro-Mechanical System) devices such as a disposable microchip for a flow cytometer and a micro-mixer, which have 3D structures. Injecting aqueous fluorescent solution in the microfluidic devices, we measured the flow in a steady state by the confocal scanning microscope. At first, we explain the principle of the confocal scanning microscope. And then we show the results from 3D visualization of microscopic flow structures using the confocal scanning microscope.

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A Study on Confocal Microscope for A Precise 3-Dimensional Surface Measurement (물체표면의 3차원 정밀형상측정을 위한 공초점 현미경에 관한 연구)

  • 송대호;안중근;강영준;채희창
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • pp.233-236
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    • 1997
  • In modem industry, the accuracy and the surface-finish requirements for machined parts have been becoming ever more stringent. Optical method in measurements is playing an important role in vibration measurement, crack and defect detection and surface topography with the advent of opto-mechatronics. In this study, the principle of the general confocal microscope is introduced for surface measurement, and the advanced confocal microscope that has better measuring speed than the traditional confocal microscope is developed. A study on improving the resolution of the advanced confocal microscope is followed. Finally, Software for data acquisition and analysis of various parameters in surface geometrical features has been developed.

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Numerical solution for multiple confocal elliptic dissimilar cylinders

  • Chen, Y.Z.
    • Smart Structures and Systems
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    • v.19 no.2
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    • pp.203-211
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    • 2017
  • This paper provides a numerical solution for multiple confocal elliptic dissimilar cylinders. In the problem, the inner elliptic notch is under the traction free condition. The medium is composed of many confocal elliptic dissimilar cylinders. The transfer matrix method is used to study the continuity condition for the stress and displacement along the interfaces. Two cases, or the infinite matrix case and the finite matrix case, are studied in this paper. In the former case, the remote tension is applied in y- direction. In the latter case, the normal loading is applied along the exterior elliptic contour. For two cases, several numerical results are provided.

Surface measurement using Confocal principle (공초점 원리를 이용한 표면 현상 측정)

  • 송대호;유원제;강영준;김경석
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • pp.51-54
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    • 2000
  • The traditional surface measuring method using confocal principle requires much time to measure an object surface since it is a scanning tool. In this paper, the upgraded confocal microscope is introduced. It is also a scanning tool but it requires 2D-scanning while the traditional one requires 3D-scanning. It means the time for measuring is considerably reduced. In addition, the measuring system is configured to increase the efficiency of beam. He-Ne laser whose frequency is 632.8nm is used for the laser source. An example of measuring result through the upgraded confocal microscope is showed.

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Scanning confocal microscope by direct oscillation of an optical fiber (광섬유의 직접 구동에 의한 공초점 현미경)

  • 김종배;류광현;박두성;노정은;권남익
    • Korean Journal of Optics and Photonics
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    • v.14 no.1
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    • pp.80-84
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    • 2003
  • We have constructed a scanning confocal microscope by directly oscillating an optical fiber in two different ways. Either a piezoelectric transducer or a tuning fork was used for the oscillation. Six frames of $640{\times}480$ pixel image were obtained in a second with piezoelectric oscillation and only one image of the same size was obtained in a second with tuning fork oscillation. Oscillation of optical fiber did not cause amy distortion of confocal images.

Measurement of the Internal Structure of an Optical Waveguide Embedded in a Flexible Optical Circuit Board by Enhancing the Signal Contrast of a Confocal Microscope

  • Lee, Won-Jun;Kim, Dae-Chan;O, Beom-Hoan;Park, Se-Geun;Lee, El-Hang;Lee, Seung-Gol
    • Journal of the Optical Society of Korea
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    • v.15 no.1
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    • pp.9-14
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    • 2011
  • In this study, the internal structure of an optical waveguide embedded in a flexible optical circuit board is observed with a confocal microscope. In order to increase the light reflection from an internal material interface with a very small index difference, and thus enhance the signal contrast, a theta microscopy scheme has been integrated into a conventional confocal microscope, and a high NA oil-immersion lens has been used. The interface reflectivity is increased from roughly 0.0015% to 0.025% by the proposed method, and the internal structure can thus be successfully measured.

Effects of Confocal Filter for Reading of Micro Holographic Data Storage (마이크로 홀로그래픽 저장장치의 공초점 필터의 효과)

  • Min, Cheol-Ki;Kim, Do-Hyung;Park, No-Cheol;Yang, Hyun-Seok;Park, Kyoung-Su;Park, Young-Pil
    • Transactions of the Society of Information Storage Systems
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    • v.6 no.1
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    • pp.25-30
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    • 2010
  • In this paper, we investigate the confocal filter for high quality reading signal of micro holographic data storage system. The confocal filter is used to remove crosstalk effects of adjacent recording-layer by multi-layer multiplexing method. To remove a noise of reading signal, we consider size and geometrical structure of filter. From the obtained results, we present optical characteristics including Fresnel and Fraunhofer diffraction effects. Finally, we propose the optimal confocal filter for good image quality and increased reflection power.

Measurement of metal materials structure by using the manufactured Scanning Confocal Microscopy (초소형 공초점 현미경의 제작과 금속의 구조 측정)

  • Seo, Myeong-Hee;Kim, Jong-Bae;Kwon, Nam-Ic
    • Journal of the Korean Society for Precision Engineering
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    • v.25 no.11
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    • pp.52-57
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    • 2008
  • We demonstrate the operation of an apparatus that we call the laser scanning confocal microscopy. It is valuable tool of the investigations for imaging process. We measured the thin metal structure through the SCM manufacture. Confocal microscopy offers several advantages including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens than conventional optical microscope. This research is manufactured of scanning confocal microscopy and after measured of metal materials structure.