• Title, Summary, Keyword: Crush Injury of Sciatic Nerve

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Effects of Low Power Laser on Pain Response and Axonal Regeneration in Rat Models with Sciatic Nerve Crush Injury

  • Lee, Hong-Gyun;Kim, Yong-Eok;Min, Kyung-Ok;Yoo, Young-Dae;Kim, Kyung-Yoon;Kim, Gye-Yeop
    • Journal of International Academy of Physical Therapy Research
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    • v.3 no.1
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    • pp.345-355
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    • 2012
  • This study purposed to examine the effect of low power laser on pain response and axonal regeneration. In order to prepare peripheral nerve injury models, we crushed the sciatic nerve of Sprague-Dawley rats and treated them with low power laser for 21 days. The rats were divided into 4 groups: normal group(n=10); control group(n=10) without any treatment after the induction of sciatic nerve crush injury; experimental group I(n=10) treated with low power laser(0.21$mJ/mm^2$) after the induction of sciatic nerve crush injury; and experimental group II(n=10) treated with low power laser(5.25$mJ/mm^2$) after the induction of sciatic nerve crush injury. We measured spontaneous pain behavior(paw withdrawal latency test) and mechanical allodynia(von Frey filament test) for evaluating pain behavioral response, and measured the sciatic function index for evaluating the functional recovery of peripheral nerve before the induction of sciatic nerve crush injury and on day 1, 7, 14 and 21 after the induction. After the experiment was completed, changes in the H & E stain and toluidine blue stain were examined histopathologically, and changes in MAG(myelin associated glycoprotein) and c-fos were examined immunohistologically. According to the results of this study, when low power laser was applied to rat models with sciatic nerve crush injury for 21 days and the results were examined through pain behavior evaluation and neurobehavioral, histopathological and immunohistological analyses, low power laser was found to affect pain response and axonal regeneration in both experimental group I and experimental group II. Moreover, the effect on pain response and axonal regeneration was more positive in experimental group I to which output 0.21$mJ/mm^2$ was applied than in experimental group II to which 5.25$mJ/mm^2$ was applied.

The Effect of Electrical Stimulation on MAP2 Expression in the Cerebral Cortex following Sciatic Nerve Crush Injury in Rat (흰쥐 좌골신경손상 후 전기 자극이 대뇌피질에서의 MAP2 발현에 미치는 영향)

  • Ahn, Eun-Young;Kim, Jin-Sang
    • The Journal of Korean Physical Therapy
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    • v.17 no.3
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    • pp.391-401
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    • 2005
  • The purpose of this study was to investigate the effect of electrical stimulation(EST) on MAP2(Microtubule Associated Protein 2) expression in cerebral cortex following sciatic nerve crush injury in rats. Twelve Sprague-Dawley adult female rats, six for control and six for experimental, were anesthetized and their sciatic nerves were crushed. The electrical stimulation (EST) was applicated with 3 Hz for 10 minuties in a day for muscles innervated sciatic nerve. The MAP2 expression in cerebral cortex was identified from immunohistochemistry against MAP2. The result of this study were as follow: 1) In control group, MAP2 immunoreactive neurons were observed but there no significant increase for 3 days. 2) MAP2 immunoreactive neurons were increased markably in experimental group than control group. 3) MAP2 immunoreactive neurons were increased markably after applicating with EST in sciatic nerve crush injury induced group from 2nd day. This study showed that the application of EST for muscles after sciatic nerve crushed injury made MAP2 immunoreactive neurons in the cerebral cortex increased. Therefore, the electrical stimulation on the peripheral site, denervated muscle, may facilitate MAP2 expression in the cerebral cortex.

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The Effects of Ohyaksungi-san(Wuyaoshunqi-san) and Electrical Acupuncture on Nerve Regeneration after Crush Injury in Rat Sciatic Nerve (흰쥐 좌골신경 압좌 손상 후 오약순기산 및 전침이 신경손상의 회복에 미치는 영향)

  • Yang, Mi-Sung;Shin, Mi-Suk;An, Hye-Lim
    • Journal of Korean Medicine Rehabilitation
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    • v.18 no.4
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    • pp.25-37
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    • 2008
  • Objectives : Ohyaksungi-san(Wuyaoshunqi-san) has been used for many years as a treatment for sciatica in oriental medicine. And electrical acupuncture also has been used as a treatment for sciatica in recent study. By the way, it is hard to find the study that apply two treatments in the same time. This study was designed to evaluate the effects of Ohyaksungi-san(Wuyaoshunqi-san) and electrical acupuncture on nerve regeneration after crush injury in rat sciatic nerve in the same time. Methods : This study was designed with 4 subgroups to evaluate the effects of Ohyaksungi-san(Wuyaoshunqi-san) and electrical acupuncture on nerve regeneration. As control groups, group I has not been treated during 3 weeks after crush injury in rat sciatic nerve. Group II has been treated Ohyaksungi-san(Wuyaoshunqi-san), Group III has been treated electrical acupuncture, and Group IV has been treated both during 3 weeks. This study has been estimated sciatic function index and change of GAP-43 immunoreactivity about sciatic nerve regeneration. Results : 1. The test for nerve regeneration had significantly good result of sciatic function index in the experimental groups as compared with control groups(p<.05). Especially, Group IV has the greatest result of sciatic function index. 2. GAP-43, the marker of nerve regeneration, more increased in the experimental groups as compared with control groups. Especially, Group IV has the greatest result of GAP-43. Conclusions : Ohyaksungi-san(Wuyaoshunqi-san) and electrical acupuncture have good effects on nerve regeneration after crush injury in rat sciatic nerve.

Improved axonal regeneration by Boyanghwano-tang treatment in mice given sciatic nerve injury (좌골신경 손상 모델에서 보양환오탕 처리에 의한 축삭 재생반응성 분석)

  • Chang, In-Ae;Kim, Ki-Joong;Namgung, Uk
    • Journal of Haehwa Medicine
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    • v.25 no.1
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    • pp.99-108
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    • 2016
  • While axons in the peripheral nerve can regenerate and lead to functional recovery to a certain extent after injury, its efficacy varies depending on the severity and duration of the injury. Here, we investigated the effects of Boyanghwano-tang (BYHOT) treatment on the regenerative responses in the sciatic nerves after prolonged transection and coaptation surgery. In mice given crush injury, axonal regeneration was completed when analyzed 1 week later and did not show any difference in regenerative reponses in the distal portion of the nerve between saline- and BYHOT-treated groups. In animal models with transection and reconnection, axonal regeneration was markedly retarded compared to animals with crush injury. Regenerating axons were extended into the reconnected distal portion of the nerve more actively in animals treated with BYHOT than saline controls. Cdc2 protein was similarly induced in nerves with crush injury and with transection and recollection, and its level was lower in BYHOT-treated animal than saline control when measured 2 weeks after nerve reconnection. These results suggest that BYHOT may be useful to promote axonal regeneration in the peripheral nerve after severe injury.

The Effects of c-Fos Expression on Ultrasound Treatment in Sciatic Nerve Crush Damaged Rats (초음파 치료가 좌골신경 압좌 손상된 흰쥐의 c-Fos 발현에 미치는 영향)

  • Kim, Dong-Dae
    • Journal of Korean Physical Therapy Science
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    • v.14 no.1_4
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    • pp.11-23
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    • 2007
  • This study was performed to evaluate the effects of low-intensity ultrasound application to the peripheral nerve injury animal model on enhancement of nerve regeneration and functional recovery. Using aseptic microsurgical techniques, the sciatic nerve of adult male Sprague-Dawley rats was crushed at the outside of right mid-thigh for 30 seconds with fine forceps. Beginning just after surgery, various continuous-wave ultrasound treatments with intensities of 0.2 W/$cm^2$, 0.5 W /$cm^2$ and 1.0 W /$cm^2$ operated at 1 MHz or sham treatment were applied to the opposite inside of the crush site for 1 minute every other day with a transducer moving speed of 2cm/sec. For evaluation of the progress of sciatic nerve regeneration, c-Fos expression in the lumbar spinal cord (L4-5) dorsal horn was investigated. c-fos expression was markedly increased at 1hour after sciatic nerve crush injury, then gradually decreased thereafter. The c-fos expressions were significantly decreased (p<0.05) in all the experimental groups in comparison with the control group until 3days post-crush, and the degrees of decrease were higher in 0.5 W/$cm^2$ and 1 W/$cm^2$ intensity ultrasound application groups. It is suggested that low-intensity ultrasound application to an animal model of sciatic crush injury may suppress pain transmission and promote nerve regeneration, and which may result in delayed progress of muscle atrophy and accelerated progress of muscle recovery and eventually may result in accelerated and improved foot function recovery.

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The Effects of Exercise Intensity and Initial Timing on Functional Recovery after Sciatic Nerve Crush Injury in Rats

  • Cai, Junyan;Na, Sang-su;Hwangbo, Gak
    • Journal of the Korean Society of Physical Medicine
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    • v.10 no.3
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    • pp.1-7
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    • 2015
  • PURPOSE: The aim of this study was to investigate the effects of exercise intensity and initial timing on functional recovery following sciatic nerve injury in rats. METHODS: Total of 80 Sprague-Dawley rats was used and randomly divided 6 groups. Under deep anesthesia, the sciatic nerve was nipped by adapted hemostatic tweezers for 30 seconds and the injured nerve was transparent under naked eyes. Acute exercise groups was applied treadmill after sciatic nerve crush injury during 5days with three type intensity. Late exercise groups was also applied treadmill during 5 days with three type intensity after 5 days break. Values of sciatic functional index were measured and analyzed in each group after exercise period. RESULTS: The sciatic functional index values between control groups 1, acute low-intensity group, acute middle-intensity group in acute phase showed statistical significant (p<.05). The sciatic functional index values between control groups 2, late low-intensity group, late middle-intensity group and late high-intensity in late phase showed statistical significant (p<.05). The comparison in acute and late phase, sciatic functional index values of each low-intensity group and each high-intensity group showed statistical significant (p<.05). CONCLUSION: Whether at acute or late phase, treadmill exercise as a therapy obtained beneficial effects of functional recovery and exercise training at low speed is more beneficial effects on the recovery of motor function in acute phase.

Influence of Transcutaneous Electrical Nerve Stimulation and Electroacupuncture on C-fos Expression in Spinal Cord and Functional Recovery After Rat Sciatic Nerve Crush Injury (경피신경전기자극과 전침자극이 흰쥐 좌골신경 압좌손상 후 척수내 c-fos 발현과 기능회복에 미치는 영향)

  • Lee, Hyun-Min
    • The Journal of the Korea Contents Association
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    • v.9 no.6
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    • pp.187-195
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    • 2009
  • The purpose of this study was to identify the effect of transcutaneous electrical nerve stimulation(TENS) and electroacupuncture(EA) after sciatic nerve crush injury in rats. Subjects were classified TENS group with TENS application, EA group with EA application and Control group which is not applicated electrical stimulation. TENS and EA stimulations were applied post-injury day(PD) 1 to 14 after sciatic nerve injury. This study observed c-fos expression in rat lumbar spinal cord. In addition, the paw withdrawal latency(PWL) and sciatic function index(SFI) were measured. The results were as follows: At PD 1, control group had higher c-fos immunoreactivity than experimental groups. At PD 7 and 14, control group had higher c-fos immunoreactivity than experimental groups. The PWL of experimental groups were significantly lower than control group. The SFI had not significant difference in all groups. But the average of experimental groups were higher than control group. These results suggest that TENS and EA applications increasing sensory and motor nerve recovery while decreasing c-fos immunoreactivity after sciatic nerve crush injury.

The Experimental Study of Electroacupuncture and Cervi Pantortichum Cornu Pharmacopuncture on Pain Decrease and Nerve Regeneration after Crush Injury of Sciatic Nerve (좌골신경 압좌 손상 후 전침 및 녹용 약침의 통증 감소와 신경 재생에 관한 실험적 연구)

  • Ahn, Hye-Lim;Yang, Mi-Sung;Shin, Mi-Sook;Choi, Jin-Bong;Kim, Sun-Jong
    • Journal of Korean Medicine Rehabilitation
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    • v.19 no.1
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    • pp.39-55
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    • 2009
  • Objectives : This study was designed to evaluate effects of electroacupuncture and Cervi Pantortichum Cornu pharmacopuncture on pain and nerve regeneration after crush injury of sciatic nerve in rats. Methods : We divided the subjects into 5 groups : control group, acupuncture treated group, electroacupuncture treated group, Cervi Pantortichum Cornu pharmacopuncture treated group, electroacupuncture and Cervi Pantortichum Cornu pharmacopuncture group. Hot plate test and degree of Substance P antibody in each group were observed at the 1st, 2nd, 3rd and 5th day. Sciatic function index and change of BDNF immunoreactivity had been estimated at the 7th, 14th, 21st day respectively. Atrophy of the gastrocnemius at the 21st day was observed. Results : All the experimental groups showed significant changes in hot plate test, degree of Substance P antibody, sciatic function index and change of BDNF immunoreactivity compared with control group. Especially, when electroacupuncture and Cervi Pantortichum Cornu pharmacopuncture used together, results were more effective than other groups. Recovery of the gastrocnemius was also the same. Conclusions : It is suggested that electroacupuncture and Cervi Pantortichum Cornu pharmacopuncture may play a significant role on pain decrease and nerve regeneration after crush injury of sciatic nerve in rats and these two treatments could be more effective when used together.

The Effect of EA and TENS on GAP-43 Expression in Spinal Cord after Rat Sciatic Nerve Crush Injury (전침자극과 경피신경전기자극이 흰쥐 좌골신경 압좌손상 후 척수내 GAP-43 발현에 미치는 영향)

  • Lee, Hyun-Min;Park, Eun-Se;Kim, Min-Hee;Kim, Souk-Boum;Kim, Dong-Hyun;Kim, Jin-Sang
    • The Journal of Korean Physical Therapy
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    • v.18 no.1
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    • pp.65-73
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    • 2006
  • Purpose: The purpose of this study was to identify the effect of electroacupuncture(EA) and transcutaneous electric nerve stimulation(TENS) after sciatic nerve crush injury in rats. Methods: The EA for experimental group I (Exp I, n=15) and TENS for experimental group II (Exp II, n=15) was applied from post-injury day(PD) 1 to PD 14 after sciatic nerve injury using low frequency stimulator that gave electrical stimulation(15min/60Hz). In order observe the effect of EA and TENS, this study examined GAP-43 expression in rat lumbar spinal cord at the PD 1, PD 7 and PD 14. In addition, the stride length(SL) and toe out angle(TOA) were measured at the PD 7 and PD 4. Results; Exp I and Exp II had higher GAP-43 immunoreactivity than control group(PD 1, 7, 14). The SL of Exp I and Exp II were significantly higher than control group(PD 7, 14). The TOA of Exp I and Exp II were significantly lower than control group(PD 7, 14). Conclusion: EA and TENS application increased motor nerve recovery and expression of GAP-43 immunoreactivity after sciatic nerve crush injury. Therefore effect of TENS and EA had similar effect on nerve regeneration and functional recovery.

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Cdc2 promotes activation of Schwann cell in regenerating axon after sciatic nerve injury in the rat. (좌골신경섬유 재생시 Cdc2 kinase 매개성 슈반세포 활성화의 역할 규명)

  • Han, In-Sun;Seo, Tae-Beom;Kim, Jong-Oh;NamGung, Uk
    • Journal of Haehwa Medicine
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    • v.14 no.1
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    • pp.201-211
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    • 2005
  • Cdc2 kinase is a prototypical cyclin-dependent kinase critical for G2 to M phase cell cycle transition. Yet, its function in the nervous system is largely unknown. Here, we investigated possible role of Cdc2 in axonal regeneration using sciatic nerve system in rat. Cdc2 protein levels and activity were increased in the injured sciatic nerves 3 and 7 days after crush injury and then decreased to basal level 14 days later. Administration of Cdc2 kinase inhibitor roscovitine in vivo at the time of crush injury significantly inhibited axonal regeneration when regrowing axons were analyzed using retrograde tracers. Cdc2 protein levels in cultured Schwann cells which were prepared from sciatic nerves 7 days after crush injury were much higher compared with those from uninjured sciatic nerves, suggesting that Cdc2 protein expression was primarily induced in the Schwann cells. To further investigate Cdc2 function in Schwann cell, we examined changes in cultured Schwann cell proliferation and migration in culture system. Both the number of proliferating Schwann cells and the extent of neurite outgrowth from co-cultured DRG neurons were significantly decreased by Cdc2 inhibitor roscovitine treatment in DRG culture which was prepared from animals with sciatic nerve injury for 7 days. Also, Schwann cell migration in the injured sciatic nerve explant was significantly inhibited by roscovitine treatment. Taken together, the present data suggest that Cdc2 may be involved in peripheral nerve regeneration via Schwann cell proliferation and migration.

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