• Title/Summary/Keyword: D-pinitol

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Anticancer effects of D-pinitol in human oral squamous carcinoma cells

  • Shin, Hyun-Chul;Bang, Tea-Hyun;Kang, Hae-Mi;Park, Bong-Soo;Kim, In-Ryoung
    • International Journal of Oral Biology
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    • v.45 no.4
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    • pp.152-161
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    • 2020
  • D-pinitol is an analog of 3-methoxy-D-chiro-inositol found in beans and plants. D-pinitol has anti-inflammatory, antidiabetic, and anticancer effects. Additionally, D-pinitol induces apoptosis and inhibits metastasis in breast and prostate cancers. However, to date, no study has investigated the anticancer effects of D-pinitol in oral cancer. Therefore, in this study, whether the anticancer effects of D-pinitol induce apoptosis, inhibit the epithelial-to-mesenchymal transition (EMT), and arrest cell cycle was investigated in squamous epithelial cells. D-pinitol decreased the survival and cell proliferation rates of CAL-27 and Ca9-22 oral squamous carcinoma cells in a concentration- and time-dependent manner. Evidence of apoptosis, including nuclear condensation, poly (ADP-ribose) polymerase, and caspase-3 fragmentation, was also observed. D-pinitol inhibited the migration and invasion of both cell lines. In terms of EMT-related proteins, E-cadherin was increased, whereas N-cadherin, Snail, and Slug were decreased. D-pinitol also decreased the expression of cyclin D1, a protein involved in the cell cycle, but increased the expression of p21, a cyclin-dependent kinase inhibitor. Hence, D-pinitol induces apoptosis and cell cycle arrest in CAL-27 and Ca9-22 cells, demonstrating an anticancer effect by decreasing the EMT.

Inhibitory Effect of D-pinitol on Both Growth and Recurrence of Breast Tumor from MDA-MB-231 Cancer Cells (D-Pinitol의 유방암 증식 및 재발 억제 효능)

  • Kim, Yoon-Seob;Park, Ji-Sung;Kim, Minji;Hwang, Bang Yeon;Lee, Chong-Kil;Song, Sukgil
    • Korean Journal of Pharmacognosy
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    • v.45 no.2
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    • pp.174-180
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    • 2014
  • D-Pinitol, an anti-diabetic substance, is a naturally occurring compound found in legumes. In this study, we investigated the inhibitory effect of D-pinitol on growth and recurrence of breast cancer. When D-pinitol was treated on MDA-MB-231 or MCF-7 breast cancer cells, it was observed that the viability of the two cancer cell lines was reduced in MTT assay. In order to examine the effect on the growth of breast tumor, mouse xenograft assay was carried out. On day 0, nine millions cells of MDA-MB-231 were injected subcutaneously into nude mouse and D-pinitol was administered orally at the dose of 500 mg/kg or 1000 mg/kg body weight for consecutive 45 days. Tumor size was reduced in dose-dependent manner upto 95.4% in 1000 mpk-treated group, compared with the non-treated control group. When D-pinitol was co-administrated with $4{\mu}g$ of doxorubicin, recurrence of breast tumor was delayed by two weeks, compared with the mouse group of doxorubicin monotherapy. Consistent with this data, it was observed that the population of cancer stem cells (CSCs), responsible for recurrence of cancer, within tumor mass was significantly reduced. Taken together, D-pinitol inhibits the growth of breast cancer and relapse of the tumor by suppressing the proliferation of CSCs.

Isolation of Gene according to the Physiological Changes of Lespedeza cuneata. G don by the Convergence Study using a Computer Program and NGS (Next Generation Sequencing) (NGS (Next Generation Sequencing)와 컴퓨터 프로그램의 융합적 연구를 통한 비수리(Lespedeza cuneata. G. don)의 생리적 변화에 따른 유용 유전자 분리)

  • Ahn, Chul-Hyun
    • Journal of the Korea Convergence Society
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    • v.8 no.12
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    • pp.31-38
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    • 2017
  • This study was carried out to investigate the possibility of isolating the useful gene of soybean plant, anthocyanin, through NGS (Next Generation Sequencing) and molecular biology experiments. Lespedeza cuneata. G. don is a resource plant but has many useful materials. Especially, D-pinitol, which has anti-diabetic function, is contained in a large amount. However, the gene related to the biosynthesis of D-piniol has not been isolated in the non-spermatid. Lespedeza cuneata. G. don was treated with abiotic stress (drought), total RNA was extracted, and a library was constructed to perform NGS. In this way, the genes involved in D-pinitol biosynthesis were isolated and sequenced in silico. In order to support this, ononitol epimerase involved in D-pinitol amplification was identified using the Blast program and RT-PCR confirmed the increased gene expression in vitro, and the gene was isolated and identified by convergence study.

D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-κB

  • Rengarajan, Thamaraiselvan;Nandakumar, Natarajan;Rajendran, Peramaiyan;Haribabu, Lingaiah;Nishigaki, Ikuo;Balasubramanian, Maruthaiveeran Periyasamy
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1757-1762
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    • 2014
  • Development of drugs from natural products has been undergoing a gradual evoluation. Many plant derived compounds have excellent therapeutic potential against various human ailments. They are important sources especially for anticancer agents. A number of promising new agents are in clinical development based on their selective molecular targets in the field of oncology. D-pinitol is a naturally occurring compound derived from soy which has significant pharmacological activitites. Therefore we selected D-pinitol in order to evaluate apoptotic potential in the MCF-7 cell line. Human breast cancer cells were treated with different concentrations of D-pinitol and cytotoxicity was measured by MTT and LDH assays. The mechanism of apoptosis was studied with reference to expression of p53, Bcl-2, Bax and NF-kB proteins. The results revealed that D-pinitol significantly inhibited the proliferation of MCF-7 cells in a concentration-dependent manner, while upregulating the expression of p53, Bax and down regulating Bcl-2 and NF-kB. Thus the results obtained in this study clearly vindicated that D-pinitol induces apotosis in MCF-7 cells through regulation of proteins of pro- and anti-apoptotic cascades.

Increase of the D-chiro-inositol and D-Pinitol Contents by Abiotic Stress in the Buckwheat Seedlings (쓴메밀 유식물에서 비 생물학적 스트레스에 의한 D-chiro-inositol과 D-pinitol의 함량 증가)

  • Ahn, Chul-Hyun;Park, Phun-Bum
    • Journal of Life Science
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    • v.19 no.10
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    • pp.1456-1462
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    • 2009
  • D-chiro-Inositol, which is the isomer of myo-inositol, is a well known drug for the treatment of type II diabetes. The methylated form of D-chiro-inositol, D-pinitol and D-chiro-inositol are synthesized when the plants are exposed to the abiotic stresses such as drought, salinity and low temperature as osmoprotectants. In soybean, myo-inositol is converted to ononitol by O-methyltransferase, and ononitol is converted to D-pinitol by ononitol epimerase and finally converted to D-chiro-inositol by demethylase. However there have been some reports that in buckwheat, myo-inositol can be converted to D-chiro-inositol directly. This study was conducted to determine the changes of soluble cyclitols in buckwheat seedlings after exposure to salt and drought stresses by GC-FID. The results indicated that myo-inositol may be the precursor of D-chiro-inositol biosynthesis.

Comparison of NaCl and D-Pinitol Content of Freeze-Dried Ice Plant, Natural and Purified Commercial Salts and their Radical Scavenging Activity

  • Sim, Wan-Sup;Park, Sung-Ho;Choi, Sun-Il;Cho, Bong-Yeon;Choi, Seung-Hyun;Han, Xionggao;Jang, Gill-Woong;Kwon, Hee-Yeon;Choi, Ye-Eun;Men, Xiao;Yoon, Sangjin;Kim, Young-Jun;Cho, Ju-Hyun;Lee, Ok-Hwan
    • Journal of Food Hygiene and Safety
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    • v.34 no.6
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    • pp.595-600
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    • 2019
  • This study investigated the suitability of freeze-dried ice plant (Mesembryanthemum crystallinum) as the source of an edible healthy salt alternative by examining the content of NaCl, D-pinitol, total phenols, total flavonoids, and DPPH radical scavenging activity compared with those of natural and purified salts. Our results showed that the NaCl content of freeze-dried ice plant, natural and purified salts was 19, 94 and 99%, respectively. The D-pinitol content of freeze-dried ice plant was 777 mg/100 g, whereas D-pinitol was not detected in either natural or purified salts. The total phenol and flavonoid contents of freeze-dried ice plant were 115 mg GAE/100 g and 985 mg RE/100 g, respectively. The DPPH radical scavenging activity of freeze-dried ice plant was markedly higher than that of natural and purified salts. Altogether, these results show that freeze-dried ice plant contains both NaCl and bioactive compounds and can be used as a source of edible salt with positive health effects.

Characteristics of Pinitol as a Functional Food Biomaterial (건강기능성 식품소재로서 pinitol의 특성)

  • Son, Min-Sik;Seo, Myung-Seon;Lee, Sang-Han
    • Journal of Life Science
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    • v.19 no.1
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    • pp.147-151
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    • 2009
  • D-pinitol, another chemical structure of 3-O-methyl-D-chiro-inositol, is an important insulin-sensitizer. The purpose of this review is to examine the characteristics of pinitol and other analogs as functional food biomaterials which were well known to reduce blood glucose levels. Pinitol can be converted to chiro-inositol in normal humans, while diabetic patients can not use the molecule, resulting in exhibiting low level of chiro-inositol in their urine. Recently, it is reported that pinitol can trigger phospholipase C/D, thus the rate of glucose metabolism accelerates to use as fuel for human body. To not only reduce insulin resistance of diabetic patients, but also alleviate the symptoms of diabetes, obesity, and muscle contraction, pinitol and its dietary supplementation is needed.

Biochemical Components and Physiological Activities of Ice Plant (Mesembryanthemum crystallinum) (아이스플랜트(Mesembryanthemum crystallinum)의 생화학적 성분 및 생리활성 효과)

  • Kang, Seungmi;Kim, Seonjeong;Ha, Suhyun;Lee, Changryul;Nam, Sanghae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.12
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    • pp.1732-1739
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    • 2016
  • The general components and cyclitol compounds of ice plant (Mesembryanthemum crystallinum) were analyzed to examine the possibility of using extracts as functional food materials, the antioxidant effects and antidiabetic activities of the extracts by solvent fraction were tested. Among the mineral contents, contents of K and Na were the largest ($1,213.33{\pm}2.52$ and $545.53{\pm}12.01mg/100g$, respectively), followed by S, Ca, P, and Mg in order of precedence. Among cyclitol compounds, content of D-pinitol was the largest ($4.04{\pm}0.08mg/g$) while contents of chiro-inositol and myo-inositol were relatively small ($2.82{\pm}0.01$ and $0.25{\pm}0.01mg/g$, respectively). Among total phenol contents by solvent fraction, contents of chloroform and ethyl acetate fractions were large ($35.80{\pm}1.33$ and $23.70{\pm}0.62mg\;GAE/g$, respectively). Among antioxidant activity levels examined by DPPH, ABTS, FRAP, and lipid/MA assays, the chloroform fraction commonly showed the highest level of activity while the ethyl acetate fraction showed relatively high levels of activity. The antioxidant activity levels were proportional to total phenol contents by solvent fraction. As for antidiabetic effects, all solvent fractions showed at least 50% ${\alpha}-glucosidase$ inhibitory activity levels while the ethyl acetate, butanol, and chloroform fractions showed high levels activity of $90.33{\pm}0.40$, $87.98{\pm}0.16$, and $86.38{\pm}0.51%$, respectively. The ${\alpha}-amylase$ inhibitory actively levels were in the range of $25.63{\pm}1.45{\sim}60.34{\pm}2.67%$, which was lower than the ${\alpha}-glucosidase$ inhibitory activity levels, but the inhibitory activity levels by solvent fraction were similar. Given the above study results, ice plant can be utilized as a natural material with antioxidative and antidiabetic functionality.

Effect of Fermented Ice Plant (Mesembryanthemum crystallinum L.) Extracts against Antioxidant, Antidiabetic and Liver Protection (아이스플랜트(Mesembryanthemum crystallinum L.) 발효추출물의 항산화, 항당뇨 및 간 보호효과)

  • Nam, Sanghae;Kang, Seungmi;Kim, Seonjeong;Ko, Keunhee
    • Journal of Life Science
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    • v.27 no.8
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    • pp.909-918
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    • 2017
  • Ice plant (Mesembryanthemum crystallinum L.) was fermented in brine in the form of mulkimchi (IPMB), and its contents of organic acid and cyclitols and biological activities were compared with those before fermentation. The pH of the IPMB continuously decreased until the sixth day of fermentation. The lactic acid yield was greatest on the fourth day. D-pinitol in ice plant mulkimchi solids (IPMS) decreased during fermentation. However, myo-inositol and D-chiro-inositol increased. The radical scavenging activities of ABTS and DPPH, in addition to the activity of FRAP, of the IPMS extract were generally higher after fermentation, with the activities highest on the fifth ($79.09{\pm}0.69%$), fourth ($87.55{\pm}1.21%$), and sixth ($78.72{\pm}0.99%$) days of fermentation, respectively, when treated with 1 mg/ml of the extract. As shown by a lipid/MA assay, antioxidant activity was generally higher after fermentation. The viability of BNL CL.2 cells damaged by t-BHP, $H_2O_2$, and ethanol was $14.19{\pm}0.98$, $13.80{\pm}2.25$, and $25.89{\pm}2.90%$, respectively. When treated with $200{\mu}g/ml$ of IPMS extract, the cell viability was $57.06{\pm}4.52%$ on the first day, and $66.06{\pm}1.36%$ on the fourth day, and $50.07{\pm}04.85%$ on the sixth day of fermentation. Hepatocyte protective effects did not increase significantly after fermentation. ${\alpha}-glucosidase$ inhibitory activity was quite high, with a range of $83.52{\pm}2.69$ to $92.79{\pm}2.16%$, and the activity increased gradually in all the groups over the fermentation period. There was no clear correlation between ${\alpha}-amylase$ inhibitory activity and fermentation.

Effect of Organic Solvent Extractives on Korean Softwoods Classification Using Near-infrared Spectroscopy

  • Yeon, Seungheon;Park, Se-Yeong;Kim, Jong-Hwa;Kim, Jong-Chan;Yang, Sang-Yun;Yeo, Hwanmyeong;Kwon, Ohkyung;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.4
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    • pp.509-518
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    • 2019
  • This study analyzed the effect of organic solvent extractives on the classification of wood species via near-infrared spectroscopy (NIR). In our previous research, five species of Korean softwood were classified into three groups (i.e., Cryptomeria japonica (cedar)/Chamaecyparis obtuse (cypress), Pinus densiflora (red pine)/Pinus koraiensis (Korean pine), and Larix kaempferi (Larch)) using an NIR-based principal component analysis method. Similar tendencies of extractive distribution were observed among the three groups in that study. Therefore, in this study, we qualitatively analyzed extractives extracted by an organic solvent and analyzed the NIR spectra in terms of the extractives' chemical structure and band assignment to determine their effect in more detail. Cedar/cypress showed a similar NIR spectra patterns by removing the extractives at 1695, 1724, and 2291 nm. D-pinitol, which was detected in cedar, contributed to that wavelength. Red pine/Korean pine showed spectra changes at 1616, 1695, 1681, 1705, 1724, 1731, 1765, 1780, and 2300 nm. Diterpenoids and fatty acid, which have a carboxylic group and an aliphatic double bond, contributed to that wavelength. Larch showed a catechin peak in gas chromatography and mass spectroscopy analysis, but it exhibited very small NIR spectra changes. The aromatic bond in larch seemed to have low sensitivity because of the 1st overtone of the O-H bond of the sawdust cellulose. The three groups sorted via NIR spectroscopy in the previous research showed quite different compositions of extractives, in accordance with the NIR band assignment. Thus, organic solvent extractives are expected to affect the classification of wood species using NIR spectroscopy.