• Title, Summary, Keyword: DPPH

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The Scavenging Effect on NO, DPPH and Inhibitory Effect on IL-4 in Radix Cynanchi Wilfordii Herbal-Acupunture Solution (백하수오약침액(白何首烏藥鍼液)의 NO, DPPH 소거(消去) 및 IL-4 억제효과(抑制效果))

  • Kim, Dong-hyun;Seo, Jung-chul;Lim, Sung-chul;Jung, Tae-young;Han, Sang-won
    • Journal of Acupuncture Research
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    • v.20 no.4
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    • pp.42-52
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    • 2003
  • Objective: For designing an optimal countermeasure against ageing and ageing related disease, it is necessary to understand the ageing mechanism and other reactive species. Free radicals are associated with the mechanisam of biological ageing and age-related diseases. This study was performed to identifiy the scavenging effect of Radix Cynanchi Wilfordii Herbal-Acupunture Solution(RCWHA) on Nitric Oxide(NO), 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) and Interleukin-4(IL -4). Methods: The activity to scavenge DPPH radicals and NO was observed in vitro, We evaluated the antioxidant activity of RCWHA by using DPPH or NO free radical method. Also the inhibitory effect on IL-4 in RCWHA was observed in vitro, Results: The followings are the summary of the results; (1) There is a significant scavenging effect of RCWHA on NO in 0.1mg/ml and 10mg/ml group after 24hrs. (2) There is no significant scavenging effect of RCWHA on DPPH. (3) There is a significant scavenging effect of RCWHA on IL-4 in $10{\mu}g/m{\ell}$ and $100{\mu}g/m{\ell}$ group. Conclusions: These findings indicate that RCWHA can be used as antioxidant or antiimflamatory drug. But further study is needed about the effect of RCWHA.

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Scavenging Effect on NO, DPPH and Inhibitory Effect on IL-4 in Astragali Radix Herbal-Acupuncture Solution (황기약침액(黃耆藥鍼液)의 NO, DPPH 소거(消去) 및 IL-4 억제효과(抑制效果))

  • Lee, Kyung-min;Seo, Jung-chul;Han, Sang-won
    • Journal of Acupuncture Research
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    • v.20 no.2
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    • pp.184-194
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    • 2003
  • Objective : This study was designed fo find out whether Astragali Radix Herbal-Acupuncture Solution(ARHA) can scavenge NO, DPPH and IL-4 or not. Astragali Radix has been clinically used to reforce the depression of immune reponse etc. in Oriental Medicine. Methods : Free radical metabolism seems to occupy a remarkably common position in the mechanisms of ageing and ageing related disease. Oxidative damage to DNA, lipids, proteins and other molecules may contribute to the development of cancer, cardiovascular disease and possibly neurodegenerative disease. The effects of ARHA on NO, DPPH and IL-4 were measured. Results : The followings are the summary of the result; (1) There is a significant scavenging effect of ARHA on NO in 1, $10mg/m{\ell}$ group in after 24hrs. (2) There is no significant scavenging effect of ARHA on DPPH. (3) There is a significant scavenging effect of ARHA on IL-4 in 1, $10,100{\mu}g/m{\ell}$ group. Conclusions : These results suggest that ARHA has significant scavenging effect on NO, IL-4 and not on DPPH. This study shows that ARHA can be used for ageing related disease and further studies are required to investigate the antioxidative effects of it.

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Assessment of Antioxidative Capacity in Relation to Seed Trails of Rice Varieties

  • Song, Hong-Keun;Ahn, Joung-Kuk;Kim, Kwang-Ho;Lee, Sun-Joo;Baek, Jin-Yeong;Chung, Ill-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.6
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    • pp.544-553
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    • 2006
  • In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r=0.29***), DPPH (r=0.80***) and TBA (r=0.76***). Between the three activities, SOD was not positively correlated with DPPH (r=0.15*), while TBA was significantly correlated with DPPH value (r=0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).

DPPH Radical Scavenging Effect and Antimicrobial Activities of Plantain (Plantago asiatica L.) Extracts (질경이 추출물의 DPPH 라디칼 소거효과 및 항균활성)

  • Jeong, Chang-Ho;Bae, Young-Il;Shim, Ki-Hwan;Choi, Jine-Shang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.10
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    • pp.1601-1605
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    • 2004
  • To develop the functional food, methanol extracts of dried plantain (Plantago asiatica L.) was fractionated in hexane, chloroform, ethyl acetate, butanol and aqueous fraction. DPPH radical scavenging effect, reducing power, nitrite scavenging effect and antimicrobial activities of these fractions were investigated. Ethyl acetate fraction among these fractions showed the highest effect of all experiments. Ethyl acetate fraction showed about 60% scavenging effect on the DPPH radicals at 400 $\mu$g/5 mL. Nitrite scavenging effect of ethyl acetate and butanol fraction was more than 70~80% at concentration above of 500 $\mu$g. Among various solvent fractions from methanol extracts of plantain, ethyl acetate fraction showed the highest inhibitory effect on the microorganism such as Staphylococcus aureus, Bacillus cereus and Vibrio parahaemolyticus at 5 mg/disc.

Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities (과산화아질산염과 DPPH 라디칼에 대한 해조추출물의 소거 활성 효과)

  • Lee, Hee-Jung;Kim, You-Ah;Park, Ki-Eui;Jung, Hyun-Ah;Yoo, Jong-Su;Ahn, Jong-Woong;Lee, Burm-Jong;Seo, Young-Wan
    • Ocean and Polar Research
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    • v.26 no.1
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    • pp.59-64
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    • 2004
  • As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for $ONOO^-$ and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at $5{\mu}g/ml$ of methanol extracts was observed in order of Ishige okamurae(95.3%), Sargassum hemiphyllum(90.2%), Symphyocladia latiuscula(89.6%), Porphyra suborbiculata(86.7%), and Gelidium amamsii(85.9%), Also, a significant scavenging effect against direct authentic peroxynitrite was revekaled for methanol extracts of Ishige okamurae(66.2%) and Sargassum hemiphyllum(55.2%) and the acetone/methylene chloride(1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride(1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.

Analysis of Total Phenolic Content and Antioxidant Activity from Fruits of Vaccinium oldhamii Miq. (정금나무 열매의 총 페놀 함량 및 항산화활성 분석)

  • Kim, Hyeusoo;Kim, Moon Sup;Kim, Sea Hyun;Yun, Kyeong Won;Song, Jeong Ho
    • Journal of Korean Society of Forest Science
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    • v.102 no.4
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    • pp.566-570
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    • 2013
  • Vaccinium oldhamii Miq. is a Korean native tree, which is deciduous and shrub tree with broad leaf. To develop superior cultivars for edible or medicinal purposes, we evaluated total phenolic content and antioxidant activity. Total phenolic content was measured by folin-denis method and antioxidant activity was measured by DPPH free radical scavenging activity (DPPH method) and reducing power (potassium ferricyanide method). Total phenolic content, DPPH free radical scavenging activity (at 400 ppm) and reducing power (at 700 ppm) were 15.8~19.9 mg/g, 69.1~91.4% and 0.56~0.96, respectively. A linear correlation was shown between DPPH-radical scavenging activity and reducing power (r=0969).

DPPH Radical Scavenging Effect and in vitro Lipid Peroxidation Inhibition by Portulaca oleracea (쇠비름(Portulace oleracea) 추출물의 DPPH radical 소거능과 in vitro 지질과산화 억제 효과와 그 활성성분)

  • 이희정;이범종;이동석;서영완
    • KSBB Journal
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    • v.18 no.3
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    • pp.165-169
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    • 2003
  • The antioxidative activity of Portulaca oleracea was tested using in vitro experimental models. Antioxidative activities were determined by measuring DPPH radical scavenging activity and lipid peroxide using 2-thiobarbituric and (TBA). The crude extract was sequentially partitioned with n-hexane, 15% aq. MeOH, EtOAc, n-BuOH, $H_2O$. A remarkable antioxidative effect was observed in the EtOAc and n-BuOH fractions. The DPPH radical scavenging effect ($IC_{50}$=17.90 $\mu\textrm{g}$/ml) of the n-BuOH soluble fraction was comparable with that of the natural antioxidant, $\alpha$-tocopherol ($IC_{50}$=6.99 $\mu\textrm{g}$/ml) and the inhibition effect of lipid peroxidation in mouse liver homogenate was similar to that of the natural antioxidant, L-ascorbic acid at a concentration of 1.0 mg/ml to 5 mg/ml.

Antioxidant Activity and Biological Properties in Extracts of Euonymus alatus (Thnub.) Sieb. (화살나무 추출물의 항산화 활성 및 생물학적 특성)

  • 서경수;임종국;박재호;김충현;정규영;정형진
    • Journal of Life Science
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    • v.13 no.1
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    • pp.1-8
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    • 2003
  • Antioxidant activity and biological properties in the MeOH extracts from different plant parts of Euonymus alatus (THNUB.) were measured by DPPH free radical scavenging ability and inhibition ability against xanthine/xanthine oxidase and proliferation in HL-60 cells. DPPH free radical scavenging activities in extracts of plant parts were high such as leaf, wing, root, seed and stem, respectively. The EtOAc fractions of plant parts were purified through LH-20 column chromatography and identified by GC/MS. LH-4 fraction and LH-5 fraction of leaf, stem and root showed stronger activities than other fractions in the inhibitor activity of DPPH and xanthine/ xanthine oxidase. $IC_{50}$ values of LH-4 fraction eluted from stem extracts showed such as 2.38 and 5.32 in DPPH and xanthine/ xanthine oxidase assay. Polyphenolic compounds were identified in purified LH-20 fractions showed highest $IC_{50}$ value in DPPH and xanthine/xanthine oxidase assay The activity of POD according to sampling time was high in root harvested in May and leaf harvested in September, respectively. The activity of SOD showed only in the extracts of stem in plant parts. SOD and POD in leaf were similar in the patterns of isozyme to those of stem. The purified extracts from Euonymus alatus (THNUB.) exerted inhibition ability of proliferation in HL-60 cells.

Determination of Antioxidant Activity of Edible Calendula Flowers by Hot-air Drying Time (열풍건조 처리시간에 따른 식용꽃 금잔화의 항산화능 검정)

  • Oh, Sang Im;Kim, Seo Young;Lee, Ja Hee;Lee, Ae Kyung
    • FLOWER RESEARCH JOURNAL
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    • v.26 no.4
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    • pp.202-208
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    • 2018
  • This experiment was conducted to determine optimal hot-air drying time for producing antioxidative calendula tea. The edible calendula was dried in hot air at $60^{\circ}C$ for 5, 10, 15 hours and Hunter's color value was measured and extracted with 95% ethanol as a solvent. The contents of total polyphenol, total flavonoid, total carotenoid and DPPH (2,2 Dipheny-1-picrylhydrazyl) radical scavenging activity were investigated. The correlation between the amount of antioxidant and antioxidant activity was analyzed. As the treatment time increased, the Hunter values of L, a, and b tended to decrease, and the L value and b value decreased significantly at 15 hours. The color change was occurred in all treatments compared with the control, and the total color difference (${\Delta}E$) was the largest at 15 hours treatment. The contents of total flavonoids, total carotenoids and DPPH scavenging activity increased in the 5 hours, but decreased in the 15 hours compared to the 10 hours. The content of total polyphenol was not changed with different treatment time. A positive correlation ($p{\leq}0.01$, r = 0.610) between carotenoids and DPPH radical scavenging activity was demonstrated. The DPPH radical scavenging activity was increased at 5 and 10 hours compared to the control and decreased at 15 hours. Therefore, 5 hours or 10 hours of treatment is appropriate and further studies are needed to determine the specific treatment time.