• Title, Summary, Keyword: Dimethyl sulfoxide

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Effects of Trypsin, Collagenase and Dimethyl Sulfoxide on Dissociation of Rat Heart Cells (배양을 위한 심근세포분리에 미치는 Trypsin, Collagenase와 Dimethyl Sulfoxide의 영향)

  • Park, Chang-Woo;Lee, Yung-Chang
    • Yeungnam University Journal of Medicine
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    • v.4 no.1
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    • pp.17-23
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    • 1987
  • New born rat heart cells were dissociated using trypsin and/or collegenase to elucidate the dissociation efficiency of these two enzymes. And the effect of dimethyl sulfoxide during and immediately after cell dissociation was also investigated to clarify the so-called protective activity of dimethyl sulfoxide on cell performance. The results can be summarized as follows. 1. Cold trypsin 18 hours pretreatment followed by warm collagenase treatment resulted best cell viability and cell yield. 2. Single, warm trypsin treatment gave the poorest result. 3. Dimethyl sulfoxide did not seem to play any protective role during or immediately after rat heart cell dissociation. It had very damaging effect on rat heart cells.

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Dimethyl sulfoxide elevates hydrogen peroxide-mediated cell death in Saccharomyces cerevisiae by inhibiting the antioxidant function of methionine sulfoxide reductase A

  • Kwak, Geun-Hee;Choi, Seung-Hee;Kim, Hwa-Young
    • BMB Reports
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    • v.43 no.9
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    • pp.622-628
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    • 2010
  • Dimethyl sulfoxide (DMSO) can be reduced to dimethyl sulfide by MsrA, which stereospecifically catalyzes the reduction of methionine-S-sulfoxide to methionine. Our previous study showed that DMSO can competitively inhibit methionine sulfoxide reduction ability of yeast and mammalian MsrA in both in vitro and in vivo, and also act as a non-competitive inhibitor for mammalian MsrB2, specific for the reduction of methionine-R-sulfoxide, with lower inhibition effects. The present study investigated the effects of DMSO on the physiological antioxidant functions of methionine sulfoxide reductases. DMSO elevated hydrogen peroxide-mediated Saccharomyces cerevisiae cell death, whereas it protected human SK-Hep1 cells against oxidative stress. DMSO reduced the protein-carbonyl content in yeast cells in normal conditions, but markedly increased protein-carbonyl accumulation under oxidative stress. Using Msr deletion mutant yeast cells, we demonstrated the DMSO's selective inhibition of the antioxidant function of MsrA in S. cerevisiae, resulting in an increase in oxidative stress-induced cytotoxicity.

Inhibition of methionine sulfoxide reduction by dimethyl sulfoxide

  • Kwak, Geun-Hee;Choi, Seung-Hee;Kim, Jae-Ryong;Kim, Hwa-Young
    • BMB Reports
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    • v.42 no.9
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    • pp.580-585
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    • 2009
  • Dimethyl sulfoxide (DMSO) is widely used in chemistry and biology as a solvent and as a cryoprotectant. It is also used as a pharmaceutical agent for the treatment of interstitial cystitis and rheumatoid arthritis. Previous reports described DMSO as being reduced by methionine-S-sulfoxide reductase (MsrA). However, little is known about the DMSO reduction capability of methionine-R-sulfoxide reductase (MsrB) or its effect on the catalysis of methionine sulfoxide reduction. We show that mammalian MsrB2 and MsrB3 were unable to reduce DMSO. This compound inhibited MsrB2 activity but did not inhibit MsrB3 activity. We further determined that DMSO functions as an inhibitor of MsrA and MsrB2 in the reduction of methionine sulfoxides via different inhibition mechanisms. DMSO competitively inhibited MsrA activity but acted as a non-competitive inhibitor of MsrB2 activity. Our study also demonstrated that DMSO inhibits in vivo methionine sulfoxide reduction in yeast and mammalian cells.

Effect of Dimethyl Sulfoxide on Economic Traits and the Change of Some Metabolic Sustances of Bivoltine Silkworm, Bombyx Mori L.

  • Nirwani, R.B.;Hugar, I.I.;Kaliwal, B.B.
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.124-129
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    • 1996
  • Oral application of dimethyl sulfoxide in different concentration (1, 3 and 5 percent) to silkworm, Bomyx mori at the fourth and the fifth instar has significantly increased the commercial characters such as larval and silkgland weights, female and male cocoon weights, their shell weights and egg production. However, larval duration, cocooning and hatching percentages did not showed any significant change, as compared with that of the corresponding parameters of the carrier control. Similarly the glycogen and protein contents of the fat body and trehalose and protein contents of the haemolymph were significantly increased in 1 and 3 percent, whereas the increase of them in 5 percent concentration did not take place significantly, except haemolymph trehalose where it was found to be significant, as compared with that of carrier control.

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Block of ATP-Sensitive $K^+$ Channels Expressed in Xenopus Oocytes by Dimethyl Sulfoxide

  • Park, Jin-Bong;Chae, Soo-Wan
    • The Korean Journal of Physiology and Pharmacology
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    • v.5 no.2
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    • pp.157-163
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    • 2001
  • The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive $K^+\;(K_{ATP})$ channel comprised of sulfonylurea receptor SUR1 and inwardly rectifying $K^+$ channel subunit Kir6.2, and the other expressing renal $K_{ATP}$ channel ROMK2. At concentrations of $0.3{\sim}10%$ (vol/vol) DMSO inhibited whole cell Kir6.2/SUR1 currents elicited by bath application of sodium azide (3 mM) in a concentration-dependent manner. The inhibition constant and Hill coefficient were 2.93% and 1.62, respectively. ROMK2 currents, however, was not affected significantly by DMSO. The results support the idea that DMSO inhibits $K_{ATP}$ channel expressed in Xenopus oocyte through a protein-specific mechanism(s) that remains to be further elucidated.

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Effects of Cryoprotectants and Diluents on Cryopreservation of the Red Seabream, Pagrus major Sperm (참돔, Pagrus major 정자의 동결보존에 미치는 희석제와 동해방지제의 효과)

  • Kho, Kang Hee
    • Korean Journal of Ichthyology
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    • v.19 no.2
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    • pp.173-177
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    • 2007
  • A series of experiments were conducted to compare the effects of various diluents and cryoprotectants on the motility and survival rate in cryopreservation of the red seabream, Pagrus major sperm. Sperm was efficiently cryopreserved using 300 mM glucose as a diluent. Two cryoprotectant, dimethyl sulfoxide (DMSO) and glycerol, were added to 300 mM glucose to formulate the extenders at concentrations between 5% and 30% by volume for freezing. The highest post-thawed sperm motility and survival rate were obtained with 10% DMSO.

Dielectric Relaxation in Ethylene Glycol - Dimethyl Sulfoxide Mixtures as a Function of Composition and Temperature

  • Undre, P.B.;Khirade, P.W.;Rajenimbalkar, V.S.;Helambe, S.N.;Mehrotra, S.C.
    • Journal of the Korean Chemical Society
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    • v.56 no.4
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    • pp.416-423
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    • 2012
  • Using time domain reflectometry, the complex dielectric spectra between 10 MHz to 20 GHz has been measured in the whole composition range at 10, 20, 30 and $40^{\circ}C$ for the binary mixtures of ethylene glycol and dimethyl sulfoxide. For all the mixtures, only one dielectric loss peak was observed in this frequency range. The relaxation in these mixtures can be described by a single relaxation time using the Debye model. A systematic variation is observed in dielectric constant (${\varepsilon}_0$) and relaxation time (${\tau}$). The excess permittivity (${\varepsilon}^E$), excess inverse relaxation time $(1/{\tau})^E$, Kirkwood correlation factor (g) and thermodynamic parameters viz. enthalpy of activation (${\Delta}H$) and Gibbs free energy of activation (${\Delta}G$) have been determined, to confirm the formation of hydrogen bonded homogeneous and heterogeneous cooperative domains, the dynamics of solute - solute interaction and the hindrance to molecular rotation in the hydrogen bonded glass forming ethylene glycol - dimethyl sulphoxide system.

Filter- Photometry of Chemiluminescence from Firefly Luciferin Intermediate M420 in Deoxygenated Dimethyl Sulfoxide

  • Shibata, Rikuo;Yoshida, Yasuhiko;Wada, Naohisa
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.290-292
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    • 2002
  • The intermediate M420 formed in a solution of firefly luciferin in deoxygenated dimethyl-sulfoxide added potassium f-butoxide was observed to emit yellow-green and red light by filter-photometry. By H-NMR, M420 was found to be deprotonated at the site where luciferin reacts with oxygen.

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