• Title, Summary, Keyword: Dog plasma

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Malignant Metastatic Plasma Cell Tumor in a Dog (개에서 전이성 악성형질세포종양)

  • 김방현;최재훈;정승우;김대용;윤화영;한홍율
    • Journal of Veterinary Clinics
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    • v.19 no.4
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    • pp.443-446
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    • 2002
  • A primary metastatic malignant plasma cell tumor was presented in a 7-year-old, female, mixed breed dog. The dog was admitted to Veterinary Teaching Hospital at College of Veterinary Medicine, Seoul National University after suffering from depression, anorexia, and dyspnea. The dog was euthanized due to poor prognosis. At necropsy, numerous, firm and tan round nodules, 1 to 2 cm in diameter were noted in the ribs. The nodules formed chain or were occasionally coalescing, causing osteolysis of the ribs. A large amount of clear fluid was present in the thoracic cavity. The spleen and liver were markedly enlarged and congested. Histologically, the nodular masses were diagnosed as plasma cell tumor which was believed to be originated from the rib. Evidence of metastasis was observed in the spleen, liver, and kidney.

Binding of Nalidixic Acid with Plasma Protein -On the Species Difference in Binding- (Nalidixic Acid와 혈장단백(血漿蛋白)과 결합(結合)에 관(關)한 연구(硏究) -동물(動物)의 종속차(種屬差)에 대(對)하여-)

  • Kim, Shin-Keun
    • Journal of Pharmaceutical Investigation
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    • v.6 no.1
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    • pp.14-17
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    • 1976
  • Binding of nalidixic acid with plasma of male and female rats, dogs, and rabbits was studied in vitro using the method of equilibrium dialysis in 1/15 mole phosphate buffer (pH 7.4). Rat plasma had the most extensive binding capacity followed by dog and rabbit plasma, and the plasma of female had more extensive capability than male in rat and rabbit but it was reversed in dog.

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Development and Validation of a Robust LC-MS/MS Method for the Simultaneous Quantification of Doxifluridine and its Two Metabolites in Beagle Dog Plasma

  • Baek, In-Hwan;Chae, Jung-Woo;Chae, Han-Jung;Kwon, Kwang-Il
    • Bulletin of the Korean Chemical Society
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    • v.31 no.8
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    • pp.2235-2241
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    • 2010
  • A reverse-phase HPLC method with detection by mass spectrometry is described for the simultaneous determination of doxifluridine and its two active metabolites, 5-fluorouracil (5-FU) and 5-fluorouridine (5-FUrd), in beagle dog plasma. The optimal chromatographic separation was achieved on a Waters $Xterra^{(R)}$ $C_{18}$ column ($4.6{\times}250\;mm$ i.d., $5\;{\mu}m$ particle size) with a mobile phase of 0.1% formic acid in a mixture of 99% methanol and purified water (99:1, v/v). The developed method was validated in beagle dog plasma with a lowest limit of quantification of $0.05\;{\mu}g/mL$ for both doxifluridine and 5-FU, and $0.2\;{\mu}g/mL$ for 5-FUrd. Doxifluridine and its two metabolites were stable under the analysis conditions, and intra- and inter-day accuracies exceeded 92.87%, with a precision variability ${\leq}11.34%$ for each analyte. Additionally, the method for quantifying doxifluridine and its two metabolites, 5-FU and 5-FUrd, in beagle dog plasma was applied successfully to the analysis of pharmacokinetic samples.

Effects of Jeo Ryong-tang water Extract on Renal Function in Rabbit and Dog (저령탕전탕액(猪令湯煎湯液)이 가토(家兎) 및 개의 신장기능(腎臟機能)에 미치는 영향(影響))

  • Jo, Sang-Seop
    • Journal of Sasang Constitutional Medicine
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    • v.2 no.1
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    • pp.213-221
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    • 1990
  • Effect of Jeo Ryong-Tang water Extract on Renal Function in Rabbit and Dog. In order to investigate the Pharmacological action of Jeo Ryoung-Tang on renal function, this study was performed in rabbit and dog, making use of it's water extract. Jeo Ryoung-Tang water extract (JRWE), when given into ear vein of rabbits, produced diuresis in a small dose, but antidiuresis in a large dose. Diuretic action of JRWE accompanied the increase of glomerular filtration rate (GFR), renal plasma flow (RPF) and amounts of $Na^+$ in exdreated in urin, but fractional excretion of filtered $Na^+$ was not changed. JRWE, when injected into proleg's vein of dog, produced diuresis, At this time, changes of renal function were similar to that of diuresis in rabbit. JRWE, when infused into a renal artery of dog, exhibited the diuresis in both kidney. It is thought that JRWE, when given into vein of rabbit or dog, induces the diuresis, and the mechanism of it's diuresis is the increase of renal plasma flow through secondary action by some endogenous humoural substance.

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Influence of Dopamine on Intrarenal Blood Flow in Dog (개의 신내 혈류에 미치는 Dopamine의 영향)

  • 고석태;강호연
    • YAKHAK HOEJI
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    • v.28 no.3
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    • pp.149-160
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    • 1984
  • In order to certify the diuretic mechanism of dopamine, this study was performed in dog. The following results were obtained. Dopamine, when given intravenously, produced diuresis, and increased glomerular filtration rate (GFR), renal plasma flow (RPF), and amount of sodium excreted in urine. When infused directly into a renal artery, dopamine elicited a marked diuresis confined only to the infused side, with concomitant rises in osmolar clearance and sodium excretion as well as a slight increase in free water clearance. Simultaneously total renal plasma flow and medullary plasma flow increased markedly with a increase of glomerular filtration rate and renal plasma flow. Medullary concentration gradient of sodium also markedly lowered in the infused kidney. These changes were not observed during mannitol diuresis and renal action of dopamine were not apparent in dog pretreated with haloperidol. From the above experimental results, it is thought that dopamine, when given into a vien or infused directly into a renal artery, induces diuresis, and the mechanism of its action is due to dual actions which are hemodynamic effect along with glomerular filtraction rate, and the increased response in the medullary blood flow.

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Liquid Chromatography-Tandem Mass Spectrometry Analysis of Riboflavin in Beagle Dog Plasma for Pharmacokinetic Studies

  • Jeong, Hyeon Myeong;Shin, Beom Soo;Shin, Soyoung
    • Mass Spectrometry Letters
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    • v.11 no.1
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    • pp.10-14
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    • 2020
  • Riboflavin is a water-soluble vitamin, which serves as a precursor to flavin mononucleotide and flavin adenine dinucleotide. This study aimed to develop a simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the quantification of riboflavin in the Beagle dog plasma. This method utilized simple protein precipitation with acetonitrile and 13C4, 15N2-riboflavin was used as an internal standard (IS). For chromatographic separation, a hydrophilic interaction liquid chromatography (HILIC) column was used with gradient elution. The mobile phase consisted of 0.1% (v/v) aqueous formic acid with 10 mM ammonium formate and acetonitrile with 0.1% (v/v) formic acid. Since riboflavin is an endogenous compound, 4% bovine serum albumin in phosphate buffered saline was used as a surrogate matrix to prepare the calibration curve. The quantification limit for riboflavin in the Beagle dog plasma was 5 ng/mL. The method was fully validated for its specificity, sensitivity, accuracy and precision, recovery, and stability according to the US FDA guidance. The developed LC-MS/MS method may be useful for the in vivo pharmacokinetic studies of riboflavin.

Elevation of Plasma NT-proBNP Concentration in a Korean Jindo Dog Infected with Dirofilaria immitis (심장사상충 감염 진도견에서 혈장 NT-proBNP 농도 증가 증례)

  • Hwang, Soo-Hyen;Park, Hyung-Jin;Seo, Kyoung-Won;Lee, Sang-Eun;Song, Kun-Ho
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.496-498
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    • 2013
  • The aim of this study is to report the observed elevation of plasma N-terminal pro-B-type natriuretic peptide (NT proBNP) concentration in a Korean Jindo Dog infected with Dirofilaria immitis. Evaluation of the animal included physical examination, thoracic radiography, ELISA kit ($PetCheck^{(R)}$, IDEXX Laboratories, USA) test and plasma NT-proBNP evaluation. The infected animal's NT-proBNP concentration was compared with those of 5 normal dogs of the same age, sex, and breed; the affected Korean Jindo dog had increased plasma NT-proBNP. To the best our knowledge, this is the first report of NT-proBNP elevation in a Korean Jindo dog with dirofilariasis.

A primo vessel-like structure in a dog with inflammatory pseudotumor

  • Cho, Sung-Jin;Hong, Sun-Hwa;Han, Sang-Jun;Kim, Ok-Jin
    • Korean Journal of Veterinary Service
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    • v.35 no.1
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    • pp.77-82
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    • 2012
  • Inflammatory pseudotumor (IPT) is a term defining a mass characterized microscopically by a proliferation of bland mesenchymal spindle cells infiltrated by diffuse mixed inflammatory cells with a predominance of plasma cells and lymphocytes. Here, we show the primo vessel-like structure of the primo-vascular system (PVS) in a dog with IPT. A 6-years old male Mongrel dog was diagnosed with an abnormal mass (diameter 5.5 cm, weight 22 g) near left preputial area. The dog was submitted to the surgical detectomy of the mass. During the surgical operation, we observed primo vessel-like material. After fixations, the masses appeared macroscopically as lipoid-like, firm, white to grey masses, measuring $5{\times}8cm$. Histologically, cellular infiltration into the muscular layers was frequently seen. The mesenchymal proliferation remained the main component of the mass and was composed of myofibroblastic-like spindle cells characterized by globular, irregular nuclei containing open chromatin and a prominent nucleolus. On the basis of the histopathologic lesions, the subcutaneous mass was diagnosed as IPT. Also, we detected a primo vessel-like structures in some areas of the IPT tissues. These were observed as novel thread-like structures and bundle of tubular structures. To our knowledge, this report is the first case of primo vessel-like structure in a dog with IPT.

Malignant Metastatic Plasma Cell Tumor in a Dog

  • Park, Jae-Hoon;Kim, Jin-Hyun;Lee, Jung-Kenn;Youn, Hwa-Young;Yoon, Jung-Hee;Kim, Dae-Yong
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • pp.28-28
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    • 2001
  • A primary metastatic malignant plasma cell tumor was diagnosed in a 7-year-old, female mixed breed dog. The dog was admitted to Veterinary Teaching Hospital at College of Veterinary Medicine, Seoul National University after suffering from depression, anorexia, and dyspnea. In radiographic examination, a large occupying mass was found at the right cranial thorax. Due to poor prognosis, the dog was euthanized. At surgery, numerous, firm, round, nodules, 1 to 2 cm in diameter were noted in the ribs. The nodules were often confluent and had cylindrical appearance. Osteolysis was associated with the nodules. A large amount of clear fluid was present in the thoracic cavity. The spleen and liver were markedly enlarged and congested. Histologically, the mass was diagnosed as plasma cell tumor which was believed to be originate from the rib. Evidence of metastasis was observed in the spleen, liver, and kidney.

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Effect of Polyporus umbellatus Fries on the Renal Function of Dog (저분(猪芬)의 개의 신장기능(腎臟機能)에 미치는 영향(影響))

  • Kang, Hyung-Yong;Ko, Suk-Tai
    • Journal of Pharmaceutical Investigation
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    • v.5 no.1
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    • pp.28-40
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    • 1975
  • Polyporus would used as diuretics. Then, for the purpose of experimentally certifying the above mention, the effect on the renal function of dog was investigated, utilizing clearance technique. Water and alcohol extracts, when injected intravenously, produced significant increases of urinary sodium and potassium, osmolar and free water clearances, and urine flow, while glomerular filtration rate and renal plasma flow remained unchanged. During diuresis produced by furosemide, addition of water extract reduced the action of furosemide and markedly renal plasma flow. It would appear that these compounds are capable of action by a different mechanism or a different site. water extract, when infused directly into a renal artery, reduced the urine flow of experimental kidney as well as renal plasma flow, and the contralateral kidney exhibited diuresis, whereas amounts of sodium and potassium excreted in urine increased on both kidney. It is surmised from those observations that Polyporus induces diuresis by inhibition the reabsorptive mechanism of renal tubules through some endogeneous humoral substances, in addition, directly reduces the renal plasma flow.

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