• Title/Summary/Keyword: ELISA antibody

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Studies on the combined inactivated oil emulsion vaccine of Newcastle disease and avian infectious bronchitis in chickens (닭의 뉴캣슬·전염성 기관지염 바이러스 혼합 불활화 오일 에멀션 백신의 생산시험)

  • Jeon, Yun-seong;Kim, Sun-joong;Seo, Ik-soo
    • Korean Journal of Veterinary Research
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    • v.30 no.1
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    • pp.79-84
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    • 1990
  • A single inoculation of combined vaccines of Newcastle disease and avian infectious bronchitis of chicken, in a form of gel-oil emulsion type (gel-OEV) was tested their immunogenecity in chickens. The results were summerized as follows: 1. Average minimum and maximum ELISA antibody titers of ND were recorded 2407 and 13144 respectively. In the case of IB, 1824 and 4496 were recorded as minimum and maximum titers. 2. The distribution of average proportional groups, in the lowest and the highest, were 1.6 and 7.0 in ND ELISA and 1.4 and 2.8 in IB ELISA antibody titers. 3. ND ELISA antibody titers were significantly increased upto 7th week after the vaccination. On the other hand, IB ELISA antibody titers were raised upto 4th week after the vaccination.

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Development of a Biological Reaction and Measurement Control System for Rapid Detection of the Insecticide Imidacloprid Residues (살충제 Imidacloprid 잔류물의 신속한 측정을 위한 생물반응 및 계측제어 시스템 개발)

  • Lim J. K.;Cho H. K.
    • Journal of Biosystems Engineering
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    • v.30 no.2 s.109
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    • pp.114-120
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    • 2005
  • In this study, a biological reaction and measurement control system was developed to rapidly measure the insecticide imidacloprid residues in agricultural products. The biological reaction part of the system was designed to include micro-pumps and valves for fluid transport, and a polystyrene covet as a reaction chamber. The measurement control part of the system consisted of a photodiode with a light-emitting diode for optical density measurement, and a control microcomputer to implement assay. Signal output was read as the rate of change in optical density at 645 nm. The sensitivity of the system was 2.2 ng/mL ($IC_50$). The system could execute a measurement cycle in about 19 minutes. Research will be continued to develop an automatic sampler fur imidacloprid residues from agricultural products.

Enzyme Immunoassay for On-line Sensing of the Insecticide Imidaclopird Residues (살충제 이미다크로프리드 잔류물의 실시간 측정용 효소면역분석법)

  • 송석진;조한근
    • Journal of Biosystems Engineering
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    • v.28 no.6
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    • pp.505-510
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    • 2003
  • In Korea, due to its broad efficacy as a systemic insecticide, imidacloprid has been widely used in rice paddies to control sucking insects, soil insects, and some chewing insects and in apple orchards to control various insects pests. To quantify the imidacloprid residue concentrations, samples are assayed in vitro using enzyme-linked immunosorbent assays(ELISA). These assays generally require several hours to perform. As a biosensor, a competitive imidacloprid ELISA was modified to measure insecticide concentrations. It was found that a total assay time of 15 min(10-min antibody-antigen binding, and 5-min substrate development) is sufficient for monitoring imidacloprid concentrations. Further work is needed to improve the sensitivity of the measurement protocol.

Seroprevalence of porcine reproductive and respiratory syndrom(PRRS) in Dangjin (당진지역 돼지생식기호흡기증후군(PRRS) 항체가 조사)

  • Kong, Shin-Koog;Lee, Gun-Taek;Lee, Kwan-Bok;Hong, Jun-Pyo;Kang, Soo-Jeong;Moon, Sun-Hwa
    • Korean Journal of Veterinary Service
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    • v.26 no.3
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    • pp.227-231
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    • 2003
  • The purpose of this study was sero-epidemiological survey of porcine reproductive and respiratory syndrom(PRRS) in Dangjin area. 411 samples from 26 pig farms were analyzed by enzyme linked immunosorbant assay (ELISA). The data indicate that 66% of the pigs and 92% of the farms showed sero-positives to the PRRS viruc. Sows showed 58% of sero-positive rate and fattening pigs showed 85% of seropositive rate. The rate of sero-positive in boars was 63%. No significant regional differences were detected in sero-epidemiological survey.

Serological survey of avian pneumovirus infection in laying hens of Gyeongbuk province (경북지역 산란계에서 avian pneumovirus 에 대한 항체가조사)

  • 김순태;김성국;조민희;김영환
    • Korean Journal of Veterinary Service
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    • v.26 no.1
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    • pp.51-56
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    • 2003
  • Avian pneumoviros(APV), also known as avian rhinotracheitis virus(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis (TRT). The aim of this study was to establish the presence or absence of antibodies to avian pneumovirus in the commercial poultry population of Korea. For this purpose, chicken serum samples were obtained and tested by an enzyme-linked immunosorbent assay (ELISA). The tested serum was collected in laying hens with reduction of egg production or normal in Gyeongbuk province. A total of 184 sera representing 42 different poultry farms of the Gyeongbuk region of Korea were included in this study. Laying hens of 16 different farms with reduction of egg production and laying hens of 26 different farms with clinically healthy at the time of serum sampling were considered positive to antibody against APV. In the farms with reduction of egg production, positive farm to antibody against avian pneumovirus were 11 of 16 different farms(68.8%) and positive sera were 47(58.8%) of 80 different serum. In the farms with clinically healthy flock, positive farm to antibody against avian pneumovirus were 12(46.2%) of 26 different farms and positive serum sample were 39(37.5%) of 104 different sera. According to the results tested to 42 different farms in 14 city, 8 of 14 city have flocks with antibody positive laying hens against APV, 1 of 14 city have antibody suspicious and 5 of 14 city shown antibody negative, respectively.

Changes of maternal antibodies in broilers vaccinated with infectious bronchitis, infectious bursal disease and Newcastle disease viruses detected by ELISA (육계에서 전염성기관지염, 전염성 F 낭병, 뉴캣슬병 백신투여에 따른 혈중항체가의 변동)

  • 고원석;백귀정;이정원;서이원;김태중;송희종;오언평
    • Korean Journal of Veterinary Service
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    • v.21 no.3
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    • pp.277-284
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    • 1998
  • Serum samples were collected from 100 breeders and their progeny 600 broilers. The breeders and broilers were vaccinated against infectious bronchitis(IB), infectious bursal disease(IBD) and Newcastle disease(ND) viruses according to general vaccination program. The antibodies in serum samples against IB, IBD and ND viruses were detected by ELISA using commercial ELISA kit. Geometric mean titer(GMT) of ELISA was monitored from 1-day-old to 35-day-old broilers and compared to that of breeder chickens. The GMT of ELISA to IB, IBD and ND was declined half level of the day old broiler's antibody titers at about 4, 9 and 4 days of age. The GMT of ELISA to IB, IBD and ND was declined than that of protective antibody titer at about 12, 11, and 15 days of age. Thereafter, the GMT of ELISA to IB, ND were declined and disappeared according to age of broilers. The GMT of ELISA to IBD was declined according to age of broilers, but at 25 days of age increased and 31 days of age increased than that of protective antibody titer. Taken together, these studies led to conclusion that time-course of antibody titers of broilers from vaccinated breeders and that of progeny broliers which vaccinated according to vaccine program. Those are very important data to design vaccine program to breeders and broilers.

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Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja;Joh, Seong-Joon;Choi, Kang-Seuk;Kim, Aeran;Seo, Min-Goo;Song, Jae-Young;Yun, Seon-Jong
    • Korean Journal of Veterinary Research
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    • v.56 no.3
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    • pp.189-192
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    • 2016
  • The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

Development of a Blocking ELISA for Measuring Rabies Virus-specific Antibodies in Animals

  • Yang, Dong-Kun;Kim, Ha-Hyun;Ryu, Jieun;Gee, Mi-ryun;Cho, In-Soo
    • Microbiology and Biotechnology Letters
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    • v.46 no.3
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    • pp.269-276
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    • 2018
  • Rabies virus (RABV)-specific antibodies in animals and humans are measured using standard methods such as fluorescent antibody virus neutralization (FAVN) tests and rapid fluorescent focus inhibition tests, which are based on cell culture systems. An alternative assay that is safe and easy to perform is required for rapid sero-surveillance following mass vaccination of animals. Two purified monoclonal antibodies (4G36 and B2H17) against RABV were selected as capture and detection antibodies, respectively. A genetically modified RABV, the ERAGS strain, was propagated and concentrated by polyethylene glycol precipitation. Optimal conditions for the RABV antigen, antibodies, and serum dilution for a blocking enzymelinked immune sorbent assay (B-ELISA) were established. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using serum samples from 138 dogs, 71 raccoon dogs, and 25 cats. The B-ELISA showed a diagnostic sensitivity of 95.8-96.3%, specificity of 91.3-100%, and accuracy of 96.0-97.2% compared to the FAVN test. These results suggest that the B-ELISA is useful for sero-surveillance of RABV in dogs, raccoon dogs, and cats.

Effect of Dietary Vitamin E Supplementation on Serum α-Tocopherol and Immune Status of Crossbred Calves

  • Samanta, A.K.;Dass, R.S.;Rawat, Mayank;Mishra, S.C.;Mehra, U.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.4
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    • pp.500-506
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    • 2006
  • An experiment was conducted with twenty crossbred male calves (7-15 days old) divided into 4 different experimental groups on the basis of body weights to study the effect of vitamin E supplementation on the serum ${\alpha}$-tocopherol concentration and immune response of the calves. All the calves were fed on milk and calf starter up to 13 weeks and afterwards, they were fed on concentrate mixture and oat hay up to 32 weeks of age. In addition, the calves in groups I, II, III and IV were supplemented with 0, 125, 250 and 500 IU feed grade DL-${\alpha}$-tocopheryl acetate, respectively. Blood samples were collected at 0 day and subsequently at 1, 2, 3, 4, 6 and 8 months of age to monitor the serum ${\alpha}$-tocopherol concentration in crossbred calves. After 24 weeks of experimental feeding, 4 animals from each group were intramuscularly inoculated with single dose (3 ml) of Haemorrhagic septiceaemia (Pasteurella multocida P52 strain) oil adjuvant vaccine. The cumulative group mean serum ${\alpha}$-tocopherol concentration (${\mu}g/100ml$) was 88.12, 210.11, 235.21 and 294.02 in-groups I, II, III and IV, respectively and differed significantly (p<0.001) among the four groups. Lymphocyte stimulation indices (LSI) did not differ among the groups significantly. The pooled mean ELISA antibody titer against Pasteurella multocida (P52 strain) was 788.02, 926.85, 1,214.00 and 1,109.51 for group I, II, III and IV, respectively, which indicated higher antibody titer in groups supplemented with vitamin E as compared to the control group. It may be concluded that vitamin E supplementation increased the ${\alpha}$-tocopherol concentration in serum and dietary supplementation of vitamin E at higher level has a humoral immune enhancing effect against killed bacterial antigen.

Comparative evaluation of two commercial ELISA kits for detection of PRRS antibodies using sera collected from pigs in various stages of PRRSV infection (다양한 PRRSV 감염상태에 있는 돼지 혈청을 이용한 PRRS 항체 ELISA 키트들의 비교 평가)

  • Seo, Byoung-Joo;Kim, Hyoun-Il;Kim, Won-Il
    • Korean Journal of Veterinary Service
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    • v.37 no.3
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    • pp.151-156
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    • 2014
  • Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses to the Korean pig industry. ELISA tests using recombinant nucleocapsid protein of PRRSV have been most commonly used for PRRS diagnostics. In the current study, two commercial PRRSV ELISA kits (Bionote PRRSV Antibody ELISA and IDEXX 3XR PRRS Antibody ELISA) have been compared using sera collected from 19 swine farms in various stages of PRRSV infection confirmed by professional diagnostic centers. Thus 130 sera collected from 5 different farms with active PRRSV infection, 130 sera from 6 different farms with PRRS-stabilized status, and 140 sera from 8 different farms with PRRS-free status were evaluated to determine the correlation of test results between those ELISA kits. Both ELISA kits showed a good correlation [PRRSV-positive farms ($R^2$=0.6375) and stabilized farms ($R^2$=0.8928)] in sample-to-positive (S/P) ratio va lues. Among the 140 sera from negative farms, one sample was falsely positive by either of the ELISA kits. In conclusion, both of the ELISA kits showed a good correlation when applied on field samples collected from farms at various stages of PRRSV infection. Bionote ELISA or IDEXX ELISA gave a false positive result on 1 out of 140 negative samples so their specificity was calculated as 99.3%. Therefore, Bionote ELISA would be a good complementary and alternative method for IDEXX ELISA kit, and vice versa.