• Title/Summary/Keyword: Edwardsiella tarda

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Transferable R plasmid of Edwardsiella tarda isolated from diseased flounders, Paralichithys olivaceus (넙치에서 분리된 Edwardsiella tarda의 약제내성 전달성 R plasmid)

  • Kim, Eun-Heui
    • Journal of fish pathology
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    • v.12 no.2
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    • pp.115-121
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    • 1999
  • MIC test of 16 chemotherapeutic agents was performed on 24 isolates of Edwardsiella tarda collected from flounders. They revealed resistance against combinations of ampicillin, amoxicillin, erythromycin, flumequine, doxycycline(DOXY), nalidixic acid, novobiocin, oxolinic acid, oxytetracycline(OTC), thiamphenicol(TP) and sulfonamide. Two strains carried transferable R plasmid encoding Otc Kanamycin Tp and Otc chloramphenicol Doxy Tetracycline Tp, respectively. The R plasmids were not similar each other on the basis of their digestion pattern of restriction endonuclease, suggesting distribution of different transferable R plasmid among E. tarda from flounders.

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The Study on the Experimental Ascite by Edwardsiella tarda in Snakehead (Channa argus) (Edwardsiella tarda에 의해 유발된 가물치 복수증에 관한 연구)

  • LEE Hun-Ku;SEONG Hee-Kyung;PARK Lee-Heon;JO Keug-Rae;KIM Young-Ja
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.5
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    • pp.353-360
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    • 1990
  • The bacterium Edwardsiella tarda was injected into healthy snakeheads (Channa argus) in order to prove the causative agent of ascite. The bacterium dominantly isolated from 2 cultured ascite snakeheads was injected into fish by the dose of $5\times10^6$ CFU/ 0.25ml, but the same dose of $0.65\%$ physiological saline was injected into the each control. The injected fish was divided into 4 groups such as intraperitoneal, intramuscle, control intra-peritoneal and control intramuscle according to their injection points. Each was composed of 10 healthy snakeheads respectively. Ascites and haemorrhagic ulcers became distinct 5 days after injection, but controls did not show any abnormal symptoms during the experimental period. Edwardsiella tarda was reisalated out of the injected fish's ascite, liver, kidney, spleen and haemorrhagic ulcer on the skin. Regardless of the injecting methods, liver was necrotized more severely than any other internal organ, but both the glomeruli of kidney and spleen were considerably damaged. Necrosis of muscle and a number of leucocytes were observed at the ulcerous region of the intramuscular injected fish. It is concluded that judging from the above results the Edwardsiella tarda is a causative agent to cause ascite in snakehead.

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Respiratory burst activity of tilapia (Oreochromis mosambicus) phagocytes to Edwardsiella tarda ghosts

  • Kim, Gi-Hong;Kwon, Se-Ryeon;Nam, Yun-Kwon;Lee, Eun-Hye;Kim, Min-Sun
    • Journal of fish pathology
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    • v.19 no.3
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    • pp.285-288
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    • 2006
  • The respiratory burst activity of tilapia (Oreochromis mosambicus) phagocytes agaisnt Edwardsiella tarda ghosts produced by gene E mediated lysis was investigated. Stimulation with E. tarda ghosts (ETG) showed markedly higher respiratory burst activity than that with formalin killed E. tarda (FKC) in phagocytes. This result may suggest that ETG can induce effective cellular immune responses in fish.

Comparative Study of Pathogenicity Following Single or Coinfection with Edwardsiella tarda and Streptococcus iniae in Olive Flounder, Paralichthys olivaceus (넙치, Paralichthys olivaceus에서 Edwardsiella tarda와 Streptococcus iniae 단독 또는 혼합감염의 병원성 비교 연구)

  • Kwon, Mun-Gyeong;Jung, Sung-Hee
    • Journal of Fisheries and Marine Sciences Education
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    • v.24 no.4
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    • pp.591-601
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    • 2012
  • Olive flounders, Paralichthys olivaceus, were intraperitoneally challenged with Edwardsiella tarda or Streptococcus iniae or both bacteria simultaneously. The pathogenicity was respectively compared with blood chemical using alanine aminotransferase (ALT), aspatate aminotransferase (AST), glucose and total protein, lysozyme activity, bacterial number of kidney and spleen, histopathological change, and cumulative mortality. The tested group of coinfection showed increased cumulative mortality, bacterial number of kidney and spleen, AST and histopathological change, but not in lysozyme activity compared with others. This study provides support for the conclusion that simultaneous infection with Edwardsiella tarda and Streptococcus iniae in a susceptible host results in higher pathogenicity, leading to the increment of bacterial number and the destruction of the internal organs.

In vitro combination effects of natural substances and antimicrobials against Edwardsiella tarda and Streptococcus iniae (In vitro에서 Edwardsiella tarda와 Streptococcus iniae에 대한 항균제와 천연 유래 항균물질의 병용효과)

  • Bak, Su-Jin;Kang, Bong-Jo;Park, Kwan Ha
    • Journal of fish pathology
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    • v.28 no.1
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    • pp.17-26
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    • 2015
  • The purpose of this study is to explore the possibility of essential oils and flavonoids improving the performance of antibiotics, proving a higher efficacy in the presence of natural substances against Edwardsiella tarda and Streptococcus iniae. Increased efficacy was observed in several cases: both essential oils and flavonoids increased antibacterial efficacy of oxytetracycline and flumequine against E. tarda by 2-fold; essential oils and flavonoids increased antibacterial efficacy of nalidixic acid against E. tarda by 4-fold; flavonoids increased antibacterial efficacy of josamycin against S. iniae by 4-fold. The results obtained in this study suggest that essential oils and flavonoids might be especially useful in increasing the antimicrobial activity of nalidixic acid and josamycin against E. tarda and S. iniae in fish. Natural compounds examined in this study could also be useful to help decrease the overuse of antibiotics in fish.

The Effect of Food Treated with Gamma Radiation after Inoculation with Pathogenic Bacteria in the Flounder (Paralichthys olivaceus) (넙치에서 방사선을 조사한 병원성 세균 오염사료 투여의 효과)

  • 김세라;김성호
    • Journal of Veterinary Clinics
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    • v.19 no.1
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    • pp.7-13
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    • 2002
  • This study was examined the microbiological adequacy of fish feed treated with high-dose irradiation (5 kGy). 125 flounder (Paralichthys olivaceus) were grouped into 5 and then the fishes were fed the following feeds for 28 days: (1)standard feed; (2)standard feed, inoculated with Edwardsiella tarda ($1{\times}10^8-1{\times}10^9CFU/g$ of feed); (3)standard feed, inoculated with Vibrio anguilarum ($1{\times}10^8-1{\times}10^9CFIT/g$ of feed); (4)standard feed, inoculated with Streptococcus faecalis ($1{\times}10^8-1{\times}10^9CFU/g$ of feed); (5)standard feed, inoculated with Edwardsiella tarda, Vibrio anguillarum and Streptococcus faecalis, and then irradiated the mixed feed to 5 kGy. The flounders feed the mixed diet with Edwardsiella tarda, Wbrio anguillarum or Streptococcus faecalis inoculated feed were showed severe cumulative mortalities of 60, 48 and 52% respectively. The gross and histological changes were observed on the fishes. However, fishs fed with the feed of bacteria inoculation before irradiation demonstrated excellent protection against the bacteria-related disease. The results from experiments with bacteria inoculated feed indicated that the irradiation methods employed were capable of preventing contamination of the fishs with pathogenic bacteria.

Detection of Edwardsiella tarda, the Pathogenic Bacteria in Freshwater Fishes by Means of the Indirect Fluorescent Antibody Technique (간접형광항체법을 이용한 담수양식어의 병원균 Edwardsiella tarda의 검출)

  • 류해진;조우영;이청산;허강준
    • Korean Journal of Veterinary Service
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    • v.16 no.2
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    • pp.111-119
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    • 1993
  • In this study, we carried out the rapid diagnostic system based on indirect fluorescent anti-body technique (IFAT) for detection of bacterial diseases in cultured freshwater fishes. 1. When the fishes were tested with graded dilution of Edwardsiella tarda FPC 470 bacteria detection from ten fishes Injected with $4.1{\times}10^3$colony forming unit(CFU) /ml, all of them were detected by IFAT but only two fishes were recognizable by the culture method in the tested fishes injected with $4.1{\times}10^3$CFU /ml. 2. The bacteria E. tarda could be detected by IFAT method from 1 to 48hrs after Injection in the tissues tested such as kidney, liver and spleen of the fishes, whereas detection by culture method could be recognized from 1 to 48hrs after injection In the kidney and spleen but it was not possible from preinjection to 1 hr in the liver. 3. Thus, IFAT proved to be more useful technique than plate culture method in the diagnosis of Edwardsiellosis in the freshwater fishes.

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Antimicrobial Activities of Alkyl Gallates Alone and in Combination with Antibiotics Against the Fish Pathogenic Bacteria Edwardsiella tarda and Vibrio anguillarum (알킬갈레이트 유도체의 어병세균 Edwardsiella tarda와 Vibrio anguillarum에 대한 항균활성 및 항생제와의 병용효과)

  • Kang, So-Young;Kang, Ji-Young;Kim, Su-Yeon;Kim, Do-Hyung;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.41 no.3
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    • pp.188-192
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    • 2008
  • Methyl gallate isolated from bark of the tree Rhus verniciflua Stokes has significant antimicrobial activity against the fish pathogenic bacteria Edwardsiella tarda and Vibrio anguillarum. To evaluate the antimicrobial activity of gallate derivatives, eight alkyl gallates were tested. Ethyl gallate and propyl gallate had the highest activities, with MICs of $15.6-31.3{\mu}g/mL$ against E. tarda. For V. anguillarum, propyl gallate and butyl gallate were highly effective, with MICs of $7.81-31.3{\mu}g/mL$. When used in combination with antibiotics, methyl gallate exhibited synergistic effects with oxytetracycline against E. tarda and with norfloxacin against V. anguillarum. These results suggest that short-chain alkyl gallates can be used as alternatives to antibiotics against the fish pathogenic bacteria.

The antimicrobial compound of Rhus verniciflua barks against fish pathogenic gram-negative bacteria, Edwardsiella tarda and Vibrio anguillarum (어류병원성 그람음성세균 Edwardsiella tarda와 Vibrio anguillarum에 대한 칠피의 항균활성물질)

  • Kang, So-Young
    • Journal of fish pathology
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    • v.18 no.3
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    • pp.227-237
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    • 2005
  • To obtain antimicrobial compounds against fish pathogenic bacteria from natural products, 80% methanolic extracts from 14 species of medicinal plant were screened for antimicrobial activity against fish pathogenic bacteria, Edwardsiella tarda and Vibrio anguillarum. Among them, Glycyrrhiza glabra, Rhus vemiciflua and Sanguisorba officinalis were effective for growth inhibition of Gram-negative bacteria, both E. tarda YSF and V. anguillarum YSR. Through the activity-guided isolation for R. verniciflua extract that exhibited the highest antimicrobial activity among three extracts, one antimicrobial compound (1) was isolated and identified as methyl-3,4,5-trihydroxybenzoate, or methyl gallate. This compound significantly inhibited the growth of tested strains of both E. tarda and V. anguillarum exhibiting MIC of 1 mg/ml for each strain.

The Efficacy and Safety on Combination Vaccines : Edwardsiella tarda, Streptococcus iniae and S. parauberis, in Olive flounder, Paralichthys olivaceus (넙치, Paralichthys olivaceus에서 Edwardsiella tarda, Streptococcus iniae와 S. parauberis 혼합백신의 효능 및 안전성 연구)

  • Kwon, Mun-Gyeong;Hwang, Jee Youn;Jung, Sung Hee
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.6
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    • pp.1193-1200
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    • 2014
  • Edwardsiella tarda, Streptococcus iniae and S. parauberis are main bacterial pathogens in aquaculture farms of olive flounder, Paralichthys olivaceus. We have discussed the efficacy and safety of 3 type-combined vaccines (A: S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, B: S. iniae 1mg + S. parauberis 1mg + E. tarda 0.5mg, C: S. iniae 1.5mg + S. parauberis 1.5mg + E. tarda 1mg) through intraperitoneal injections in olive flounder. None of the vaccines gave rise to any signigicant side effects on histopathology and blood chemistry. The antibody titers and lysozyme activities of A type were higher than those of B, C and control. Four weeks after vaccination, RPS (relative percent survival rates) was 62.5~75% (A type), 50~66.7% (B type) and 55.6~62.5% (C type) respectively. As the results, the combined vaccines are possible to prevent edwardsiellosis and streptococcosis, and A type : S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, is the most effective out of them.