• 제목, 요약, 키워드: Ellagic

검색결과 79건 처리시간 0.034초

Ellagic Acid Exerts Anti-proliferation Effects via Modulation of Tgf-Β/Smad3 Signaling in MCF-7 Breast Cancer Cells

  • Zhang, Tao;Chen, Hong-Sheng;Wang, Li-Feng;Bai, Ming-Han;Wang, Yi-Chong;Jiang, Xiao-Feng;Liu, Ming
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.1
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    • pp.273-276
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    • 2014
  • Ellagic acid has been shown to inhibit tumor cell growth. However, the underlying molecular mechanisms remain elusive. In this study, our aim was to investigate whether ellagic acid inhibits the proliferation of MCF-7 human breast cancer cells via regulation of the TGF-${\beta}$/Smad3 signaling pathway. MCF-7 breast cancer cells were transfected with pEGFP-C3 or pEGFP-C3/Smad3 plasmids, and treated with ellagic acid alone or in combination with SIS3, a specific inhibitor of Smad3 phosphorylation. Cell proliferation was assessed by MTT assay and the cell cycle was detected by flow cytometry. Moreover, gene expression was detected by RT-PCR, real-time PCR and Western blot analysis. The MTT assay showed that SIS3 attenuated the inhibitory activity of ellagic acid on the proliferation of MCF-7 cells. Flow cytometry revealed that ellagic acid induced G0/G1 cell cycle arrest which was mitigated by SIS3. Moreover, SIS3 reversed the effects of ellagic acid on the expression of downstream targets of the TGF-${\beta}$/Smad3 pathway. In conclusion, ellagic acid leads to decreased phosphorylation of RB proteins mainly through modulation of the TGF-${\beta}$/Smad3 pathway, and thereby inhibits the proliferation of MCF-7 breast cancer cells.

Effects of Gellan, Xanthan, and $\lambda$-Carrageenan on Ellagic Acid Sedimentation, Viscosity, and Turbidity of 'Campbell Early' Grape Juice

  • Ghafoor, Kashif;Jung, Ji-Eun;Choi, Yong-Hee
    • Food Science and Biotechnology
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    • v.17 no.1
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    • pp.80-84
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    • 2008
  • The effect of gellan (GE), xanthan (XA), and $\lambda$-carrageenan (LC) on the viscosity, sedimentation, ellagic acid content, and turbidity of grape 'Campbell Early' juice (CEJ) was investigated. CEJ samples with 0.15% each of GE, XA, and LC were tested for the above variables after 0, 5, 10, and 20 days of storage. The samples containing GE (0.15%) showed the least amount of sediment formation, the lowest ellagic acid content and turbidity, and a rise in viscosity. Sedimentation in CEJ decreased with increased viscosity due to the addition of gums which also limited the ellagic acid content and turbidity. GE was the most effective additive for the stabilization of CEJ.

Determination of Flavonoids, Tannins and Ellagic Acid in Leaves from Rubus L. Species

  • Gudej, Jan;Tomczyk, Michal
    • Archives of Pharmacal Research
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    • v.27 no.11
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    • pp.1114-1119
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    • 2004
  • This paper describes the quantitative determination of flavonoids, tannins and ellagic acid in the leaves from wild and cultivated variations of Rubus L. species (Rosaceae): raspberry (2 wild and 13 cultivars) and blackberry (3 wild and 3 cultivars). The content of flavonoids was analyzed using spectrophotometric (the Christ-M llers method) and HPLC analysis after acid hydrolysis. The content of tannins was determined by the weight method, with hide powder, described by German Pharmacopoeia 10 (DAB 10). Ellagic acid content was examined using the HPLC method after acid hydrolysis. Flavonoid content, determined using the Christ-Muller's method was higher for the blackberry leaves than for the raspberry leaves and varied between 0.46% and 1.05%. Quercetin and kaempferol were predominant in all samples analyzed using the HPLC method. The highest flavonoid content was found in the leaves of R. nessensis (1.06%); with results in all of the examined samples varying between 0.27% and 1.06%. The concentration of ellagic acid in all species was determined after acid hydrolysis and ranged from 2.06% to 6.89%. The leaves of raspberries are characterized by greater amounts of tannins (varying between 2.62% and 6.87%) than the leaves of other species. The results from this study indicate that the analyzed species are a rich source of flavonoids, ellagic acid and tannins, which may be used for the quality assessment of Rubus L. species leaves.

식품 중 Ellagic acid의 발암수식효과 (Modifying Effects of Ellagic Acid in Food on Carcinogenesis)

  • 장동덕;신동환;홍충만;조재천;한정희
    • 한국식품위생안전성학회지
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    • v.13 no.1
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    • pp.29-33
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    • 1998
  • 의약품과 식품을 포함한 자연이나 환경 속에 포함되어 있는 여러 가지 화학물질들은 암 및 돌연변이를 유발할 수 있기 때문에 이런 것들에 의한 유해작용을 줄이려 노력하고 있으나 완전히 없기에는 어려운 현실이다. 따라서 식생활 습관을 개선하거나 식품 내에 존재하는 발암억제물질은 이용하여 암의 발생을 줄이기 위한 연구는 암의 치료제 개발과 더불어 관심의 대상이 되고 있다. 여러 가지 식품 속에 자연적으로 함유된 ellagic acid는 항돌연변이와 발암억제 효과가 있는 것으로 잘 알려져 있다. 따라서 본 실험에서는 단기간에 ellagic acid의 발암 억제 효과를 알아보기 위하여 전암지표효소인 GST-P 양성증식소를 측정하였다. Diethylnitrosamine으로 간장에서 암을 유발하였고 phenobarbital 과 간부분절제술로 암을 촉진시켰으며, ellagic acid를 400과 800ppm 투여군으로 구분하고 투여시기를 달리하여 실험하였다. 실험결과 암이 유발되기 전부터 실험종료까지 ellagic acid 400 ppm 투여군의 동물에서만 발암 억제효과를 관찰 할 수 있었으나, 800ppm 투여군에서는 투여시기에 관계없이 종양억제효과가 나타났다. 따라서 Diethylnitrosamine으로 유발된 간장의 발암은 ellagic acid에 의해 용량 의존적으로 억제됨을 알 수 있었다.

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성숙도에 따른 복분자 열매의 미백 활성 비교 (Comparison of whitening effect of Rubus coreanus fruit according to maturity)

  • 박정용;이지연;서경혜;장귀영;이승은;지윤정;김형돈
    • Journal of Nutrition and Health
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    • v.53 no.2
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    • pp.121-128
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    • 2020
  • 본 연구에서는 복분자 열매의 성숙도에 따른 미백 활성의 차이를 확인하기 위해 B16F10 cell에서 melanin 생성량을 측정하고, 기능성분인 ellagic acid의 함량 변화를 확인하였다. 또한 ellagic acid의 함량에 따른 tyrosinase 저해 활성도 측정하였다. B16F10 cell에서 MRF 및 IRF 추출물은 200 ㎍/mL 농도까지 세포 독성을 나타내지 않았고, Melanin 생성량은MRF 추출물에 비해 IRF 추출물에서 더 좋은 억제 활성을 보여주었다. 또한 복분자 성숙도에 따른 ellagic acid의 함량 변화를 확인한 결과, MRF 추출물에 비해 IRF 추출물에서 ellagic acid의 함량이 높게 측정되었고, tyrosinase 저해 활성 평가에서도 MRF 추출물에 비해 IRF 추출물에서 더 좋은 억제 활성을 보였다. 결과를 종합해보면, MRF 추출물에 비해 IRF 추출물에서 ellagic acid의 함량이 더 높았으며, 이로 인해 tyrosinase의 저해 및 melanin 생성 억제 또한 IRF 추출물에서 더 좋은 것으로 판단된다. 따라서, 복분자 미성숙과는 천연 미백 소재로써 우수한 기능성 화장품 원료가 될 것이라 생각한다.

포제에 따른 지유의 지표성분 함량분석 (Quantitative Analysis of the Three Marker Compounds in Sanguisorbae Radix by Processing Method)

  • 서창섭;김정훈;신현규;김병수
    • 생약학회지
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    • v.46 no.4
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    • pp.342-351
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    • 2015
  • In this study, we performed quantitative determination of the three marker compounds such as gallic acid, ellagic acid, and quercetin in the 70% ethanol extracts of non-processed Sanguisorbae Radix and processed Sanguisorbae Radix using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the three compounds was used a Gemini $C_{18}$ column ($5{\mu}m$, $4.6{\times}250mm$) by the gradient elution with distilled water and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate and injection volume were $1.0{\mu}L/min$ and $10{\mu}L$. The concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix were 0.25, 0.26, and 0.007%, respectively, while the concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix 0.14-0.55, 0.27-2.03, and 0.001-0.007%, respectively. Among the three components, the amount of the ellagic acid was increased after processing in Sanguisorbae Radix.

Unique Phenolic Sulphate Conjugates from the Flowers of Tamarix amplexicaulis

  • Souleman, Ahmed A.M.;Barakat, Heba H.;Hussein, Sahar A.M.;El-Mousallamy, Amani M.D.;Nawwar, Mahmoud A.M.
    • Natural Product Sciences
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    • v.4 no.4
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    • pp.245-252
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    • 1998
  • The unique sulphated phenolics, gallic acid 3-methyl ether 5-potassium sulphate, isoferulic acid 3-potassium sulphate, and ellagic acid 4,4'-dimethyl ether 3-potassium sulphate have been isolated from the flowers of Tamarix amplexicaulis Ehrenb. (Tamaricaceae). The hitherto unknown natural phenolic acid, gallic acid 3-methyl ether, together with the known phenolic, gallic acid, gallic acid 4-methyl ether, isoferulic acid, ferulic acid, ellagic acid, and ellagic acid 4,4'-dimethyl ether have been also separated and characterized. The structures were established by conventional methods, including electrophoretic analysis and confirmed by ESI-MS, $^1H-\;and\;^{13}C-NMR$.

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HPLC-UVD를 이용한 수련(Nymphaea tetragona Georgi)의 Ellagic Acid 정량 분석 (Quantitative Analysis of Ellagic Acid in Nymphaea tetragona Georgi Root by HPLC-UVD)

  • 이동영;정은주;전병주;성상현
    • 생약학회지
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    • v.45 no.1
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    • pp.84-87
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    • 2014
  • A high performance liquid chromatography (HPLC) method for the quantitation of ellagic acid in Nymphaea tetragona was developed for the quality control of functional cosmetic ingredient, the extract of N. tetragona. Separation and quantitation were successfully achieved with a Kromasil C18 column ($5{\mu}m$, $250mm{\times}4.6mm$, i.d.) by isocratic elution of a mixture of acetonitrile containing 0.1% trifluoroacetic acid and water containing 0.03% phosphoric acid at a flow rate of 1.0 ml/min. The UV detector was used for the detection and the wavelength for quantitation was set at 254 nm. The presence of ellagic acid in the extract was determined by comparison of retention time and spiking with authentic standard. Analytical results showed good linearity ($R^2=0.99996$) in relatively wide concentration ranges. The R.S.D. for precision test was less than 3.0%. Recovery of the compound was 98.55~101.72% with R.S.D values less than 4.0%. In conclusion, this method has been successfully applied to the determination of ellagic acid in N. tetragona.

고창 복분자의 기능성원료 표준화를 위한 지표성분으로서 Ellagic Acid의 분석법 개발 (Method Development of Ellagic Acid as Marker Compound for Standardization of Gochang Bokbunja (Rubus coreanus Miquel) as Functional Ingredient)

  • 김윤정;한송희;전지영;황민호;임용진;채수완;김민걸
    • 한국식품영양과학회지
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    • v.41 no.11
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    • pp.1554-1558
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    • 2012
  • 고창 복분자의 개별인정형 건강기능식품 기능성 원료로 개발하기 위하여 지표성분 표준화를 위한 ellagic acid의 분석법 설정과 분석법에 대한 밸리데이션을 실시하였다. 1% formic acid가 첨가된 water와 acetonitrile을 이동상으로 하고 Symmetry$^{(R)}$ (C18, $4.6{\times}250mm$, $5.0{\mu}m$) 칼럼을 사용하여 기울기용리(gradient elution) 방법으로 분석하였다. 본 연구에서는 분석법을 확립하고 분석법에 대하여 특이성, 직선성, 정확성과 정밀성 그리고 회수율에 대하여 확인하였다. Ellagic acid의 검량선은 $R^2=0.9996$으로 좋은 선형성을 보였으며 LLOQ와 LOD는 각각 $1{\mu}g/mL$$0.3{\mu}g/mL$였다. 일내와 일간 분석에서 상대표준편차(RSD)는 각각 2.28%와 2.84%미만으로 나왔다. 회수율 측정결과에서는 89.17~97.92%로 나왔고, RSD는 0.05~0.14%였다. 그러므로 HPLC를 이용한 ellagic acid의 분석법이 고창 복분자 추출물의 기능성원료 표준화를 위한 지표성분 분석을 위한 적합한 시험법임이 검증되었다. 본 시험법에 따라 분석한 고창 미숙과 복분자 추출물 내의 ellagic acid의 함량은 세 lot를 3번씩 분석하였을 때 약 $1.92{\mu}g/mg$(0.192%)이 나왔으며 RSD값은 2.36% 이하였다. 따라서 본 연구를 통하여 확립된 ellagic acid의 분석법이 고창 복분자의 개별인정형 건강기능식품 기능성 원료 개발을 위한 기초자료로 활용될 것으로 본다.

국내산 참나무속 수종 수피의 추출성분 (Chemical Constituents of Domestic Quercus spp. Barks)

  • 김진규;권동주;임순성;배영수
    • 목재공학
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    • v.38 no.4
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    • pp.359-374
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    • 2010
  • 국내산 낙엽성 참나무류인 신갈나무, 상수리나무, 떡갈나무, 졸참나무, 갈참나무 및 굴참나무 수피의 추출성분의 구조를 규명하고 수종 상호간 성분의 특성 및 연관성 등을 조사하였다. 그 결과 신갈나무에서 화합물 2(ellagic acid, 0.03 g), 4 ((+)-catechin, 4.59 g), 6 (taxifolin, 3.35 g) 및 7 (glucodistylin, 20.52 g)을 상수리 나무에서는 화합물 1 (gallic acid, 0.18 g), 4 ((+)-catechin, 8.52 g), 5 ((+)-gallocatechin, 0.09 g), 6 (taxifolin, 0.54 g) 및 7 (glucodistylin, 3.28 g)을 떡갈나무에서는 화합물 1 (gallic acid, 0.38 g), 2 (ellagic acid, 0.11 g) 4 ((+)-catechin, 2.01 g), 5 ((+)-gallocatechin, 0.12 g) 및 7 (glucodistylin, 0.39 g)을 갈참나무에서는 2 (ellagic acid, 1.51 g), 4 (+)-catechin, 21.91 g) 및 7 (glucodistylin, 3.91 g)을 졸참나무에서는 2 (ellagic acid, 0.84 g), 4 ((+)-catechin, 0.82 g), 6 (taxifolin, 4.02 g) 및 7 (glucodistylin, 21.50 g)을 굴참나무에서는 1 (gallic acid, 0.24 g), 3 (caffeic acid, 0.05 g), 4 ((+)-catechin, 0.32 g) 및 7 (glucodistylin, 0.65 g)을 분리하여 구조를 규명하였다. 국내산 참나무속 6 수종의 수피에서는 화합물 4 ((+)-catechin)와 7 (glucodistylin) 이 공통적으로 분리되었으며 두 성분 중 함유량이 상대적으로 높은 glucodistylin은 참나무류 수피의 지표성분 으로 이용될 수 있을 것이다.