• Title, Summary, Keyword: Ellagic

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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site (HPLC에 의한 산지별 한국산 석류과피 중 항산화화합물의 함량분석)

  • Kwak, Hye-Min;Jeong, Hyun-Hee;Song, Bang-Ho;Kim, Jong-Guk;Lee, Jin-Man;Hur, Jong-Moon;Song, Kyung-Sik
    • Applied Biological Chemistry
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    • v.48 no.4
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    • pp.431-434
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    • 2005
  • The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

Antioxidative Activity of the Extract from the Inner Shell of Chestnut

  • SON Kyung Hun;YANG He Eun;LEE Seung Chul;CHUNG Ji Hun;JO Byoung Kee;KIM Hyun Pyo;HEO Moon Young
    • Biomolecules & Therapeutics
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    • v.13 no.3
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    • pp.150-155
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    • 2005
  • The ethanolic extract of chestnut (Castanea crenata S. et Z., Fagaceae) inner shell (CISE) and one of its components, ellagic acid (EA), were evaluated for their protective effects against 1, 1-diphenyl-2-picryl hydrazine (DPPH) free radical generation and hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line. CISE and EA were shown to possess the free radical scavenging effect against DPPH radical generation, significantly. They were also found to strongly inhibit hydrogen peroxide-induced DNA damage from Chinese hamster lung (CHL) cell, assessed by single cell gel electrophoresis assay and 8-hydroxy -2'-deoxy guanosine (8-OH-2'dG) assay. Furthermore, topical application of CISE [$12.5\%$(w/w) cream] and ellagic acid [$1.0\%$(w/w) cream] for 14 days potently inhibited malondialdehyde (MDA) formation of mouse dorsal skin (a marker of lipid peroxidation) induced by ultraviolet B exposure. Therefore, CISE and its component, ellagic acid, may be the useful natural antioxidants by scavenging free radicals, inhibition of lipid peroxidation and protecting oxidative DNA damage when topically applied.

Activity Guided Isolation of Antioxidant Tannoid Principles from Anogeissus latifolia

  • Govindarajan, Raghavan;Vijayakumar, Madhavan;Shirwaikar, Annie;Rawat, Ajay Kumar Singh;Mehrotra, Shanta;Pushpangadan, Palpu
    • Natural Product Sciences
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    • v.11 no.3
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    • pp.174-178
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    • 2005
  • Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.

Phenolic Acid Composition and Antioxidative Activity of Chestnut Endoderm (밤 삽피의 페놀산 조성과 항산화 활성)

  • Kim, Young-Chan;Kim, Mi-Yeon;Chung, Shin-Kyo
    • Applied Biological Chemistry
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    • v.45 no.3
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    • pp.162-167
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    • 2002
  • Free, soluble esterified and insoluble bounded phenolic acids were separated from Eungi chestnut endoderm. The composition and contents of phenolic acid were analyzed by gas chromatography, and their antioxidant activity was examined by DPPH assay, 2-deoxyribose oxidation, and ferric thiocyanate method. Gallic, ellagic, salicylic, and gentisic acids in free phenolic acid fraction, gallic, ellagic, and protocatechuic acids in soluble esterified fraction, sianpic and gentisic acids were the major phenolic acids in insoluble bounded fraction. Marked differences were observed in the phenolic acid composition and contents among the fractions. Free phenolic acid fraction showed the strongest antioxidant activity. Results revealed chestnut endoderm could be a potential antioxidant source containing gallic and ellagic acids.

Entrapment of Ellagic Acid in Dairy Protein-Based Nanoparticles

  • Lee, Mee-Ryung
    • Journal of Dairy Science and Biotechnology
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    • v.36 no.2
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    • pp.121-124
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    • 2018
  • Ellagic acid (EA) is a naturally occurring polyphenolic compound in vegetables, nuts, and fruits such as berries. EA has antioxidant, anticancer, anti-allergy, and anti-inflammatory activities. The objectives of this research were to investigate the physicochemical properties of nanoparticles before and after nano-encapsulation of EA in dairy protein and to develop a functional (anti-inflammatory) dairy protein-based beverage containing EA. A particle size analyzer was used to determine the physicochemical and morphological properties. High performance liquid chromatography was used to evaluate the entrapment efficiency of EA. The nanoparticles containing EA were 100 to 200 nm in diameter. The determined poly dispersity index value of 0.3 to 0.4 indicated that the nanoparticles were uniformly distributed with similar size. Zeta-potential values were also similar between the control groups. The entrapment efficiency of EA was nearly 90%. The results indicate the potential for development of nanoparticles containing EA beverage products with anti-inflammatory activity.

Antioxidative Constituents and Activities of Acorn hull and Chestnut Hull (도토리와 밤 외피의 항산화 성분 및 활성)

  • 차배천;이혜원;임태진
    • YAKHAK HOEJI
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    • v.47 no.4
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    • pp.212-217
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    • 2003
  • We have carried out the antioxidative activity of nuts species for the development of antioxidant from natural products. From our previous report, EtOAc and n-BuOH extracts of acorn hull and chestnut hull were found to have a strong antioxidative activity in various antioxidant experiment. In the continuous study, we isolated several compounds from EtOAc and n-BuOH extracts of acorn hull and chestnut hull by fractionation using column chromatography. The structures of isolated compounds were identified as catechin, naringenin and ellagic acid on the basis of their spectroscopic properties and by comparison of their physical and spectra data with published value. Antioxidative activities of catechin, naringenin and ellagic acid were measured by DPPH, ferric-thiocyanate and Rancimat method.

The Antioxidant Activities of Three Solvent(Ether, Butanol, Water) Extrats from Chestnut Inner Shell in Soybean Oil (대두유에 대한 율피의 용매분획별(Ether, Butanol, Water) 항산화효과)

  • Oh, Seung-Hee;Kim, Yong-Wook;Kim, Myoung-Ae
    • Journal of the Korean Society of Food Culture
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    • v.20 no.6
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    • pp.703-708
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    • 2005
  • This study was carried out to compare the antioxidant activities of the ether, butanol, water extracts from chestnut inner shell(Castanea crenata Sieb. et Zucc.) with those of tocopherol and BHA in soybean oil. All additives were added to soybean oil on the quantities of 0.02%. Comparing the antioxidant activities under autooxidation condition at $45.0{\pm}0.5$ for 42days of the extracts were recorded in the order of butanol>ether>control>BHA>tocopherol depending on the solvent. Under the condition at $60.0{\pm}0.5^{\circ}C$ for 32days, the butanol extracts represented the high antioxidation effect, however, there was no significant differences between the ether extracts and control. Under thermal oxidation condition, the ether extract showed stronger antioxidant activity than those of the butanol extract. In the results of polyphenol compound analysis, ellagic acid, quercetin, morin, naringenin, flavanol were included in the ether extracts and ellagic acid, naringenin, gallic acid, flavanol were included in the butanol extracts, respectively. Among them, ellagic acid in ether extract and gallic acid and naringenin in butanol extracts seed to increase the antioxidant activities in the substrate oil.

PROTECTIVE EFFECTS OF ELLAGIC ACID AND INNER SHELL OF CHESTNUT ON HYDROGEN PEROXIDE-INDUCED OXIDATIVE DNA DAMAGE

  • Lee, Seung-Chul;Yang, He-Eun;Jo, Byoung-Ki;Kim, Hyun-Pya;Heo, Moon-Young
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.110-110
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    • 2001
  • Inner shell of chestnut (Castanea Mollissima, Fagaceae) is well-known anti-wrinkle agent, which has been used for long time in the treatment of skin aging. In this study, the extract of chestnut inner shell (CMIE) and its major component, ellagic acid (EA), were studied for their protective effects against free radical generation and hydrogen peroxide-induced oxidative DNA damages in the mammalian cells.(omitted)

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Chemical Investigation of the Constitutive Phenolics of Rosa arabica; the Structure of a New Dimeric Phenolic Glycoside

  • Souleman, Ahmed M.A.;El-Mousallamy, Amani M.D.
    • Natural Product Sciences
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    • v.6 no.2
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    • pp.82-85
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    • 2000
  • The aqueous ethanolic whole plant extract of Rosa arabica was found to contain the new natural dimeric phenolic compound, ellagic acid 3,3'-dimethyl ether $4-O-{\alpha}-rhamnopyranoside$, 9, along with ten known phenolic metabolites (1-8, 10 and 11). Structures of all compounds (1-11) were established by routine methods of analysis and confirmed by FAB-MS, $^1H\;and\;^{13}C$ NMR spectral analysis.

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Composition of Secondary Metabolites in Various Parts of 'Seolhyang' Strawberry Plants

  • Kim, Dong Sub;Na, Haeyoung;Kwack, Yurina;Kim, Sung Kyeom;Heo, Jeong Wook;Chun, Changhoo
    • Horticultural Science & Technology
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    • v.31 no.2
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    • pp.224-230
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    • 2013
  • The objective of this study was to identify the content of phenolic and volatile organic compounds in edible and non-edible parts of 'Seolhyang' strawberry plants. We performed a comparative chemical analysis of the compounds found in roots, leaves, petioles, runners, and unripe and ripe fruits during vegetative propagation and reproductive growth. The contents of ellagic and gallic acids in the leaves of runner plants during vegetative propagation were $7.36{\pm}1.10$ and $5.07{\pm}3.66mg{\cdot}g^{-1}$ FW, respectively, and were higher than those in the other parts. The main volatile organic compound was identified as 3-hexen-1-ol, and it was mostly detected in leaves. The content of ellagic acid in leaves during reproductive growth was $12.96{\pm}2.30mg{\cdot}g^{-1}$ FW, while that in the other parts was below $6.00mg{\cdot}g^{-1}$ FW. The content of gallic acid in unripe fruits was $2.75{\pm}0.48mg{\cdot}g^{-1}$ FW and was higher than that in the other parts. Ripe fruits contained the lowest contents of ellagic and gallic acids but contained the most diverse volatile organic compounds, including sesquiterpenes, among the tested plant parts. The results indicate that non-edible parts (e.g., leaves and unripe fruits) of strawberry plants can be used as a raw material for antioxidant and anti-inflammatory agents, and edible parts (i.e., ripe fruits) can be available for making an essential oil.