• Title, Summary, Keyword: Enterococcus faecium

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Fermentation Properties of Amylase Activity and Added Rice Yogurt of Enterococcus faecium KHM-11 Isolated from Korean Human Milk (한국인 모유로부터 분리한 Enterococcus faecium KHM-11의 Amylase 활성과 쌀 첨가 요구르트의 특성)

  • Bae, Hyoung-Churl;Lee, Jo-Yoon;Renchinkhand, Gereltuya;Nam, Myoung-Soo
    • Korean Journal of Agricultural Science
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    • v.37 no.3
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    • pp.387-392
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    • 2010
  • This studies were carried out to assess fermentation properties of amylase activity and added rice yogurt of Enterococcus faecium KHM-11 isolated from Korean human milk. The amylase activity of Enterococcus faecium KHM-11 was above 23 units. Characterization of carbohydrate fermentation of Enterococcus faecium KHM-11 has D-ribose, D-lactose, L-arabinose and starch. Titratable acidity and viable count of lactic acid bacteria of 4% rice yogurt was higher compared to the 0%. Therefore we were discussed Enterococcus faecium KHM-11 is suitable microorganism for fermented milk added rice powder. Hydrolysates of sugars of fermented milk with 4% rice powder cultured Enterococcus faecium KHM-11 were analyzed by TLC and HPLC. Hydrolysates of lactose and galactose were revealed and hydrolysates of glucose was not revealed in results of TLC and HPLC.

Rapid Fermentation Starter Enterococcus faecium of Soybean for Soy-Sauce Like Product (간장태 신속 대두발효 종균으로의 Enterococcus faecium)

  • Lee, Young-Duck;Park, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.188-195
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    • 2012
  • To produce rapidly the traditional Kanjang soy sauce-like product with rich flavors, lactic acid bacteria of Enterococcus spp. isolated from Chungkukjang was used as one of starter cultures. Among 119 Enterococcus spp., eight strains were selected by protease-secreting activities and identified as four E. faecium, three E. faecalis, and one E. gallinarium. The strains showed low resistances toward eight antibiotics and had no resistant genes to the vancomycin. Especially, E. faecium O24 was cultivated well on 5% NaCl medium that was selected for further study as the starter. E. faecium O24 grew well on the steamed soybean and the counts increased by ten times overnight, which produced mostly 80 mg% glutamic acid and aspartic acid as the seasoning amino acids on the product. Various organic acids including principal lactic acid were also produced. Flavors of maltol and guaiacol, typical soy-sauce flavor, were produced in the mixed cultures of Zygosaccharomyces rouxii and Candida versatilis. Therefore, E. faecium O24 could be a starter of soybean fermentation for soy sauce-like product with rich flavors rapidly.

In vitro Activity of Sodium Benzoate Against Clinically Relevant Enterococcus faecalis and Enterococcus faecium Isolates

  • Karabay Oguz;Kocoglu Esra;Ince Nevin;Sahan Tufan;Ozdemir Davut
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.129-131
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    • 2006
  • The antimicrobial effects of sodium benzoate against Enterococcus faecalis and Enterococcus faecium were investigated. The $MIC_{90}$ of sodium benzoate were 64 mg/ for E. faecalis and 32 mg/L for E. faecium, while the $MBC_{90}$ were 128 mg/L and 64mg/L, respectively. Although further studies are required for clinical evidence, sodium benzoate seems to be effective against Enterococcus spp.

Isolation and Characterization of Temperate Phages in Enterococcus faecium from Sprouts (새싹채소 유래 Enterococcus faecium으로부터 Temperate Phage의 분리와 특성)

  • Lee, Young-Duck;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.46 no.3
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    • pp.323-327
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    • 2014
  • To analyze the characteristics of bacteriophages in Enterococcus faecium, D-19 and F6 phages were induced from five E. faecium isolated from sprouts by the treatment with mitomycin C. The bacteriophages of D-19 and F-6 had long, non-contractile tails and icosahedral heads, and were members of Siphoviridae family. As the host spectrum, D-19 phage lysed five out of 55 strains of E. faecium, whereas F6 phage lysed only three strains. Both D-19 and F6 phages displayed similar and high stabilities against ethanol and pH capable of resisting the exposure to 100% ethanol and pH 4.

Genomic Insight into the Salt Tolerance of Enterococcus faecium, Enterococcus faecalis and Tetragenococcus halophilus

  • Heo, Sojeong;Lee, Jungmin;Lee, Jong-Hoon;Jeong, Do-Won
    • Journal of Microbiology and Biotechnology
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    • v.29 no.10
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    • pp.1591-1602
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    • 2019
  • To shed light on the genetic basis of salt tolerance in Enterococcus faecium, Enterococcus faecalis, and Tetragenococcus halophilus, we performed comparative genome analysis of 10 E. faecalis, 11 E. faecium, and three T. halophilus strains. Factors involved in salt tolerance that could be used to distinguish the species were identified. Overall, T. halophilus contained a greater number of potassium transport and osmoprotectant synthesis genes compared with the other two species. In particular, our findings suggested that T. halophilus may be the only one among the three species capable of synthesizing glycine betaine from choline, cardiolipin from glycerol and proline from citrate. These molecules are well-known osmoprotectants; thus, we propose that these genes confer the salt tolerance of T. halophilus.

Draft genome sequences of Enterococcus faecium JB00008 (KACC 92186P) isolated from Korean fermented soybean paste (Cheonggukjang) (한국 전통유래식품(청국장)에서 분리한 Enterococcus faecium JB00008 (KACC 92186P) 유산균주의 유전체 분석)

  • Park, Jongbin;Jin, Gwi-Deuk;Kim, Eun Bae
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.171-173
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    • 2018
  • Enterococcus faecium was commonly used as a probiotics and feed additives to human and animals because of their beneficial effects. We sequenced the genome of E. faecium JB00008 (KACC 92186P) isolated from a Korean fermented soybean paste (Cheonggukjang) that showed antibacterial activity against Escherichia coli. A 2,847,295-bp draft genome was obtained, and it has in 37.84% G + C content in 34 contigs (length, ${\geq}500bp$).

Isolation and Antibiotic Susceptibility of Enterococcus spp. from Fermented Soy Paste (콩발효식품에서의 장구균과 항생제 내성특성)

  • Kang, Tae-Mi;Park, Jong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.5
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    • pp.714-720
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    • 2012
  • To evaluate the antibiotic risk of $Enterococcus$ in fermented soy paste, $Enterococcus$ spp. were isolated and identified from 31 $Cheongkukjang$ and 17 $Doenjang$, samples. Exactly 123 $Enterococcus$ spp., 119 from $Cheongkukjang$ and four from $Doenjang$, were ultimately isolated. The most frequently collected $Enterococcus$ isolates in $Cheongkukjang$ were 69 strains of $E.$ $faecium$ and 20 strains of $E.$ $faecalis$. All four $Enterococcus$ spp. from $Deonjang$ were identified as $E.$ $faecium$. All isolates were sensitive to ampicillin, chloramphenicol, penicillin, and tetracyclin $E.$ However, they showed broad spectra from sensitivity to resistance to erythromycin, ripampin, and streptomycin. Vancomycin minimum inhibition concentration (MIC) of $Enterococcus$ spp. from $Cheongkukjang$ ranged from 0.25 to 8 ${\mu}g/mL$. Almost all strains were sensitive to vancomycin, but eight strains showed intermediate resistance to vancomycin. Seventeen strains showing the highest MIC of 8 ${\mu}g/mL$ among all isolates were evenly distributed among $E.$ $faecalis$, $E.$ $faecium$, $E.$ $gallinarum$, and $E.$ $casselifalvus$, in which the strong resistant genes of $van$A and $van$B for vancomycin were not detected. Overall antibiotic resistance of $Enterococcus$ isolates was relatively low and particularly low vancomycin resistance was similar to those of $Enterococcus$ isolates obtained from other foods. Therefore, the antibiotics resistance of $Enterococcus$ and especially vancomycin-resistant $Enterococcus$ spp. from $Cheongkukjang$ and $Doenjang$, is not hazardous.

Characteristics of Enterococcus faecium isolated from rough-toothed dolphin, Steno bredanensis (뱀머리돌고래, Steno bredanensis에서 분리된 Enterococcus faecium의 특성)

  • Kim, Myoung Sug;Choi, Hye Sung;Han, Hyun Ja;Kim, Doo Nam;An, Du Hae;Jung, Sung Hee
    • Journal of fish pathology
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    • v.26 no.3
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    • pp.289-294
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    • 2013
  • Enterococcus faecium was isolated from the internal organs of the rough-toothed dolphine, Steno bredanensis which was stranded at Jeju island. E. faecium were isolated from the liver, spleen, kidney, heart and lung up to $1.54{\times}10^6$ cfu/g. No significant differences of bacterial enzyme activities between E. faecium KCCM 12118 and the isolates were found. Biochemical constellation was the same or similar to that of E. faecium KCCM 12118 according to API20 strep. All isolates had the multi-drug resistance to 6 antibiotics by an agar disk diffusion method but these isolates didn't have resistance to chloramphenicol and vancomycin.

Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans

  • Sim, Insuk;Park, Keun-Tae;Kwon, Gayeung;Koh, Jong-Ho;Lim, Young-Hee
    • Journal of Microbiology and Biotechnology
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    • v.28 no.6
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    • pp.883-892
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    • 2018
  • Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and $TNF-{\alpha}$) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.

Prevalence and Characterization of Vancomycin-Resistant Enterococci in Chicken Intestinles and Humans of Korea

  • Seong, Chi-Nam;Shim, Eun-Sook;Kim, Shin-Moo;Yoo, Jin-Cheol
    • Archives of Pharmacal Research
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    • v.27 no.2
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    • pp.246-253
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    • 2004
  • The prevalence, genotype for antibiotic resistance and antibiotic susceptibility of vancomycin resistant enterococci (VRE) were determined. And molecular typings of the Enterococcus faecium isolates were analyzed. Prevalence of VRE in chickens, healthy children and intensive care unit (ICU) patients was 41.6%,7.9%, and 20.4%, respectively. Forty out of 54 isolates from chicken intestines, and 9 out of 11 from ICU patients were identified as Enterococcus faecium. Eleven out of 13 isolates from non-hospitalized young children were E. gallinarium. Twelve strains of E. faecalis were isolated from chicken intestines. The gene for the antibiotic resistance in E. faecium, and E. faecalis was vanA, while that in E. gallinarium was vanC1. E. faecium isolates were resistant to most of antibiotics except ampicillin and gentamicin. Molecular typing of the E. faecium strains obtained by pulse field gel electrophoresis and repetitive sequence-based PCR suggest that VRE transmit horizontally from poultry to humans, especially young children, via the food chains in Korea.