• Title/Summary/Keyword: Enzyme Linked Immunosorbent Assay(ELISA)

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Characteristics of Tobacco Mosaic Virus Isolated from Wasabi (Eutrema wasabi) in Korea

  • Kim, Hyung-Moo;Lee, Kui-Jae
    • The Plant Pathology Journal
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    • v.15 no.4
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    • pp.247-250
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    • 1999
  • Wasabies showing mosaic symptoms were collected and extracted for virus purification. Tobacco mosaic virus (TMV) was identified as causal agent by electron microscopy and nucleic acid and coat protein analyses. TMV strains were determined by enzyme-linked immunosorbent assay (ELISA). TMV was identified as W and C strain in wasabi. The results of host reaction indicated that this virus induced local lesions on Nicotiana tabacum cv. Bright Yellow and N. glutinosa, leaf spots on Chenopodium amaranticolor and mosaic symptoms on wasabi. Rot shape virus particles were observed and was about 300 nm in length. About 6.5 kb single RNA molecule was observed from extracted viral RNA sample and 26 KDa coat protein was detected in denatured acrylamide gel. Infection ratio of TMV was 8% for the first cultivation year, but was 22% for the second year when TMV-W antiserum was used. The results of this experiment showed that infection ratios of both TMV-W and TMV-C strains were higher compared to that of TMV-P strain.

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Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) and porcine circovirus-2 (PCV-2) in pig farms in Gyeongbuk province (경북지역 양돈장의 돼지생식기호흡기증후군, 돼지써코바이러스-2 항체가 조사)

  • Sohn, Jun-Hyung;Shin, Sung-Ho;Kim, Soon-Tae;Lee, Sung-sam;Yun, Mun-Jo;Cho, Gil-Jae
    • Korean Journal of Veterinary Service
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    • v.38 no.3
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    • pp.163-166
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    • 2015
  • The purpose of this study was to survey seroprevalence of porcine reproductive and respiratory syndrome (PRRS) virus and porcine circovirus-2 (PCV-2) in Gyeongbuk province by enzyme-linked immunosorbent assay (ELISA). A total of 966 samples collected from 21 pig farms were tested. The sero-positive rate of PRRS and PCV-2 were 77.6% (750/966) and 76.4% (738/966), respectively.

High seroprevalence of Ornithobacterium rhinotracheale in layer chickens in Gyeonggi province, South Korea (경기도 산란계에서 Ornithobacterium rhinotracheale의 높은 항체 양성률)

  • Jung, Kwang
    • Korean Journal of Veterinary Service
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    • v.43 no.4
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    • pp.257-259
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    • 2020
  • Ornithobacterium rhinotracheale (ORT) causes pneumonia, airsacculitis, and pleuritis in chickens and other avian species. Little is known about the seroprevalence of ORT in layer chickens in Gyeonggi province, South Korea. The purpose of this study was to determine the seroprevalence of ORT in layer chickens in Gyeonggi province, South Korea from May to September 2019. A total of 460 chickens in 28 flocks were tested for antibodies to ORT by using commercial enzyme-linked immunosorbent assay (ELISA) kit. The seroprevalence of ORT antibodies in the flocks was 100% (28/28) and the overall seroprevalence in individual chickens was 98.91% (455/460). This survey indicated the high seroprevalence of ORT in layer chickens in Gyeonggi province, South Korea. Therefore, measures should be executed to control ORT in layer chickens in this province.

Seroprevalence of reticuloendotheliosis virus infection in layer chickens in Gyeonggi province, South Korea (경기도 산란계의 세망내피증 바이러스 항체 양성률 조사)

  • Jung, Kwang
    • Korean Journal of Veterinary Service
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    • v.43 no.3
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    • pp.197-200
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    • 2020
  • This study was performed to determine the seroprevalence of reticuloendotheliosis virus (REV) infection in layer chickens in Gyeonggi province, South Korea. Serum samples were collected from 820 layer chickens on 56 flocks in Gyeonggi province. The samples were tested for specific antibodies against REV using commercial enzyme-linked immunosorbent assay (ELISA). The seroprevalence of REV antibodies in the flocks was 62.50% (95% Confidence interval (CI) 49.33~74.40) and the overall seroprevalence in individual chickens was 39.27% (95% CI 35.97~42.65). The results of the present survey indicate that REV infection is prevalent in layer chickens in Gyeonggi province, South Korea. Therefore, effective measures should be taken to prevent and control REV of layer chickens in this province.

The Production of Antibody Against Sterigmatocystin Produced by Aspergillus vericolor (Aspergillus vericolor가 생산하는 sterigmatocystin에 대한 항체생산)

  • 윤원한;하우송;강진순;여명재;전향숙;정덕화
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.1-6
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    • 1995
  • In order to establish the enzyme linked immunosorbent assay(ELISA) of sterigmatocystin produced by Aspergillus versicolor, we experimented and obtained following results. Two of three rabbits which had been immunized with sterigmatocystin-hemiacetal-BSA produced antibodies against sterigmatocystin at 15 weeks. The produced antibodies were specific for sterigmatocystin and sterigmatocystin-hemiacetal but didn't cross react with other sterigmatocystin analogues in a significant degree. DMF : 4% KC1 (18 : 2) mixed solution was most effective to dissolve sterigmatocystin. For the preparation of sample solution to determine sterigmatocystin by ELISA, sample was extracted with CHC13 and dried, than the dried sample was redissolved with 100 ${mu}ell$ DMF + 4% KC1 mixture. 10~1,000 ng/$m\ell$ level of standard sterigmatocystin could be applied to the established ELISA. When artifically contaminated rice were assayed by the ELISA, the average recovery of sterigmatocystin spiked to 25~500 ng/g was 109% (97~116%), and mean interwell coefficient of variation was 21% (11~28%).

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Development of an ELISA kit for the detection of residual sulfadimethoxine in edible animal products (축산물 잔류 sulfadimethoxine 검출용 ELISA kit 개발)

  • Kim, Woo-taek;Kim, Seong-hee;Yoon, Byoung-su;Lim, Yoon-kyu
    • Korean Journal of Veterinary Research
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    • v.40 no.3
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    • pp.601-609
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    • 2000
  • An enzyme linked immunosorbent assay (ELISA) was developed to screen residues of sulfadimethoxine (SDM) in edible animal products. An indirect competitive ELISA was allowed to compete with rabbit anti-SDM for binding to a limited amount of SDM-gelatin conjugate and SDM in serum samples. Sera was diluted 20 times with phosphate buffered saline (PBS) and boiled for 5 minutes to destruct immunoglobulins of serum. Detection limit of this competitive ELISA for SDM was 0.1 ppb or less. Among eight sulfonamide analogues tested for specifity, only sulfamonomethoxine showed significant cross-reaction in the assay. The EC-50 value for sulfamonomethoxine was 3.5 ppm. Recovery of SDM in spiked serum samples between 100 ppb and 500 ppb ranged from 110.7% to 128.9%.

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Survey on the seroepidemiology of canine herpesvirus infection in Korea (한국에서의 canine herpesvirus감염실태에 대한 혈청역학적 조사)

  • Seo, Il-bok;Seong, Whan-woo;Lim, Chang-hyeong
    • Korean Journal of Veterinary Research
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    • v.34 no.3
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    • pp.647-652
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    • 1994
  • This study was carried out to investigate the actual condition of canine herpesvirus(CHV) infection in Korea. A total of 338 serum samples were collected randomly from the breeding and companion dogs in the local areas in Korea. The serum samples were used to determine the actual condition of the canine herpesvirus infection in Korea using enzyme linked immunosorbent assay(ELISA). The mean prevalence of CHV infection in dogs was 37% and that of the breeding and companion dogs was 58% and 28%, respectively. The prevalence of CHV infection in Seoul, Kyung-gi, Chung Nam, Cheon Nam and Pusan was detected 23%, 28%, 18%, 28% and 70%, respectively. The prevalence of CHV infection in less and more than 6 months old dogs, and in male and female dogs was 26% and 40%, 42% and 33%, respectively. These results indicate that the incidence of CHV infection is high in Korea, especially breeding dogs and older dogs.

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Enzyme Immunoassay for Rapid Detection of the Fungicide Iprovalicarb Residues (살균제 Iprovalicarb 잔류물의 신속한 검출을 위한 효소면역분석법)

  • Cho, H.K.;Kyung, K.S.;Lee, E.Y.
    • Journal of Biosystems Engineering
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    • v.31 no.6
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    • pp.535-540
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    • 2006
  • For a biosensor development, an enzyme-linked immunosorbent assay (ELISA) of the fungicide iprovalicarb was developed by minimizing the processing time. The time for whole incubation process was reduced from 135 minutes to 15 minutes. The concentration of antibody was varied to improve sensitivity. The total processing time was reduced from 2.5 hours to 20 minutes, the final sensitivity ($IC_{50}$ value) of 7.93 ng/mL and the lowest detection limit of 0.045 ng/mL were obtained. This ELISA was applied to potatoes and onions, and the recoveries were in the range of 98.85 $\sim$ 101.20% and 87.97 $\sim$ 102.70%, respectively. Accordingly, this method can be used as basis for a biosensor for rapid monitoring of iprovalicarb residues in crops.

Purification of Odontoglossum Ringspot Virus by DEAE-Cellulose Chromatography (DEAE 셀루로오즈 컬럼 크로마토그래피 기법에 의한 Odontoglossum 윤문 바이러스의 정제)

  • 이철호;박종오;정효원;나용준
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.559-562
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    • 1998
  • Odontoglossum ringspot virus (ORSV) was finally purified from ORSV-infected orchid plants by diethylaminoethyl (DEAE) cellulose anion exchange column chromatography. The virus was reliably eluted by potassium chloride at the concentration from 0.1 M to 0.13 M. Partial purification was done by solubilization with Triton X-100 (allkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG; MW 8,000). The finally purified ORSV represented one distinct homogeneous band and the molecular weight of its capsid protein was about 17,500 Dalton in electrophoretic analysis. Electron microscopy showed not only intact particles ranged from 280 nm to 340 nm in length, but also segmented particles that final 140 nm to 220 nm and even disks. Enzyme-linked immunosorbent assay (ELISA) showed that final yield was 12 mg/100 g of the infected leaves. Bioassay demonstrated that the purified ORSV had the normal infectivity to orchid plants and Nicotiana glutionsa. Based on these data, anion exchange column chromatography could be efficiently applied to the purification of ORSV and other viruses similar to ORSV.

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Development and evaluation of surface plasmon resonance imaging for the detection of antibodies against classical swine fever virus in swine

  • Cho, Ho-Seong;Lee, Tae-Uk;Park, Nam-Yong
    • Korean Journal of Veterinary Service
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    • v.30 no.2
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    • pp.205-209
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    • 2007
  • A protein chip based on surface plasmon resonance (SPR) imaging was developed for measuring classical swine fever virus (CSFV) antibody using a recombinant gp55 protein as an antigen. The diagnostic potential of SPR imaging for detecting antibodies to the CSFV gp55 protein was compared with that of a enzyme -linked immunosorbent assay (ELISA) using 70 pig sera. There was a strong positive correlation between the SPR imaging and ELISA (n=70, r=0.916, p<0.01). Therefore, the SPR imaging, which is a label-free and high-through put method, is expected to be a valuable tool in the serodiagnosis of CSFV.