• Title, Summary, Keyword: Ephedra Sinica

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The Effects of Fermentated Ephedra sinica on Obese Rats Fed by High Fat Diet (발효마황이 고지방식이로 유발된 비만 흰쥐에 미치는 효과)

  • Shin, Young-Jin;Kim, Ho-Jun;Lee, Myeong-Jong;Keum, Dong-Ho
    • Journal of Korean Medicine Rehabilitation
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    • v.19 no.4
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    • pp.37-57
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    • 2009
  • Objectives : This study was performed to investigate the effects of fermented Ephedra sinica (FMH) nano-extract compared Ephedra sinica (MH) on 3T3 L1 cell viability, lipid and glycometabolism, Obes rats' behavior and state-trait anxiety. Methods and materials : Each of 6 rats was divided into a normal diet, HFD, MH, and FMH. We fed a HFD group of rats a high fat diet and administered normal saline for 8 weeks. And we fed an experimental group of rats a high fat diet and administered an extract of Ephedra sinica and fermentated Ephedra sinica for 8 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. Ephedra sinica and fermentated Ephedra sinica was examined in effects of 3T3 L1 cell viability, lipid and glycometabolism, rats' behavior and state-trait anxiety. Results : 1. Ephedra sinica and fermentated Ephedra sinica didn't induce cytotoxicity at 3L3 L1 cell. 2. Fermentated Ephedra sinica by Lactococcus confusus inhibit $PPAR-{\gamma}$ activation and promote $TNF-{\alpha}$ activation. 3. In MH and FMH group, the body weight and FER decrease occured significantly than in HFD group. 4. No side effect of Ephedra sinica appeared in FMH group, so fermentated Ephedra sinica have a capacity of anti-anxiety. 5. Serum total cholesterol, triglyceride, HDL-cholesterol and creatine of HFD+Ephedra sinica, HFD+LO group were decreased compared with that of HFD group. Decreasing rates of sample group was significant(p<0.05). 6. In both MH and FMH group serum HDL was increased(P>0.05). 7. In MH group only TG was decreased, but in FMH group total cholesterol, TG, and glucose were decreased(P>0.05). Conclusions : Fermentated Ephedra sinica by Lactococcus confusus was superior to Ephedra sinica in capacity of anti-obesity. Further fermentated Ephedra sinica have no Ephedra sinica's side effect.

The Study on the Whitening Effect of Ephedra sinica Extract (마황추출물의 미백효과에 관한 연구)

  • Yoo Yung-Geun;Joung Min-Seok;Choi Song-Wan;Kim Joong-Hoi
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.2
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    • pp.153-159
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    • 2005
  • In this study, we investigated the application of an extract from Ephedra sinica which has been composed of traditional Korean medicine as a whitening ingredient. The extract of Ephedra sinica which was obtained from the mixture of methanol and water (1:1) the inhibitory effect of tyrosinase. Then, Ephedra sinica was extracted by two different solvents. One was water and the other was methylene chloride. Only, the water extract of Ephedra sinica showed the inhibitory effort of tyrosinase; the anti-tyrosinase activity with $0.2\%$ of the water extract was $60.6\%$. But the extract of Ephedra sinica in methylene chloride fraction showed little inhibitory effect on tyrosinase. The inhibitory effect of the concentrated water extract of Ephedra sinica was tested on L-DOPA auto-oxidation and melanin synthesis in B-16 melanoma. In L-DOPA auto-oxidation, $0.5\%$ of the concentrated water extract showed $87\%$ of inhibition of L-DOPA auto-oxidation and the $0.75\%$ concentrated Ephedra sinica extract in wafer fraction inhibited $98.8\%$ of that. In melanin synthesis of B-16 melanoma, the concentrated water effect of Ephedra sinica inhibited $70.2\%$ or $79.9\%$ of inhibitory effect on that at the concentration of $0.05\%$ or $0.075\%$, respectively. For verifying the skin whitening effect of the concentrated water extract of Ephedra sinica in vivo, we performed the clinical test of that. The research showed the significant skin whitening effect of a cream containing $0.5\%$ Ephedra sinica extract and the statistical analysis showed a significant difference (p < 0.05) between sample (containing $0.5\%$ Ephedra sinica extract) and placebo after 10 weeks.

Stimultaneous Determination of Ephedrine Alkaloids in Ephedra sinica and Wolbigachul-tang by High Performance Liquid Chromatography (고성능 액체 크로마토그래피를 이용한 마황 및 월비가출탕 전탕액에서 에페드린류의 동시분석)

  • Song, Miyoung;Kim, Jung-Ok;Leem, HyunHee;Kim, Hojun
    • Journal of Korean Medicine for Obesity Research
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    • v.20 no.2
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    • pp.97-108
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    • 2020
  • Objectives: Ephedra sinica and Wolbigachul-tang which contains Ephedra sinica are used to treat obesity in Korean medicine. The aim of this study was to analyze the quantities of ephedrine alkaloids by high-performance liquid chromatography. Methods: The analysis was performed using a YMC-Triat C18 column with operating at 25℃, and UV detection at 210 nm. The mobile phase used a gradient flow with 0.1% H3PO4 in water and acetonitrile. Specificity, linearity, precision, accuracy, limit of detection, and limit of quantification were measured for validation anaylsis. This method was applied to analyze the quantities of ephedrine alkaloids in Ephedra sinica and Wolbigachul-tang. Results: The concentration per Ephedra sinica (gram) of ephedrine and pseudoephedrine in Ephedra sinica decoction are 4.74±0.22 mg and 2.19±0.10 mg, respectively and in Wolbigachul-tang decoction are 6.39±0.34 mg and 2.97±0.21 mg, respectively. The retention time of ephedrine was 23.6 min and that of pseudoephedrine was 25.8 min, and norephedrine and methylephedrine were not detected. Conclusions: In conclusion, analyzed the concentration of ephedrine alkaloids in Ephedra sinica and Wolbigachul-tang by the developed validation method.

A Case Study of Nocturnal Enuresis in Two Children by Focusing Sleep-wake Transition (수면-각성이행에 초점을 둔 소아 야뇨증 치험 2례)

  • Jeon, Bo Ram;Min, Sang Yoen;Kim, Jang Hyun
    • The Journal of Pediatrics of Korean Medicine
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    • v.30 no.1
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    • pp.1-8
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    • 2016
  • Objectives The purpose of this study is to report the case of nocturnal enuresis in two children treated by using hypothesis that awareness during sleep may improves symptoms. Methods Enuresis children usually are not even aware of nocturnal urea due to excessive sleep. During the sleep cycle, enuresis children do not tend to have sleep-wake transition period compared to normal children. Ephedrine, the main ingredient of Ephedra sinica (麻黃), has a wakening effect. Two pediatric patients, who were suffering from nocturnal enuresis, were administered by Tosatang-gamibang (菟絲湯加味方) added Ephedra sinica (麻黃) or Boatang-gamibang (補兒湯加味方) added Ephedra sinica (麻黃) while correcting unhealthy voiding habit. Results As a result of the treatment, two pediatric patients were recovered without relapse. Conclusions In this report, we have proven that Tosatang-gamibang (菟絲湯加味方) added Ephedra sinica (麻黃) or Boatang-gamibang (補兒湯加味方) added Ephedra sinica (麻黃) and correcting voiding habit can considerably be effective in recovering nocturnal enuresis.

The Effect of Ephedra sinica Pharmacopuncture on Lipid Metabolism in an Experimental Mouse Model of Obesity (마황약침(麻黃藥鍼)이 비만 유발 생쥐의 지방대사에 미치는 영향)

  • Kim, Hyo-jae;Kim, Eun-ji;Han, Yang-hee
    • The Journal of Internal Korean Medicine
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    • v.37 no.4
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    • pp.579-590
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    • 2016
  • Objectives: This study aimed to investigate the impact of Ephedra sinica pharmacopuncture on the weight and lipid metabolism of obese mice.Methods: Obesity was induced in male C57BL/6 mice by a 60% fat diet. The animals were divided into three groups (n=5) fed a normal diet, high-fat diet, and high-fat diet with Ephedra sinica pharmacopuncture. After 13 wk, fasting blood sugar levels were measured in each group, and oral glucose tolerance tests were conducted. After 15 wk, body weight, epididymal fat pad weight, subcutaneous fat pad weight, and serum lipid and gene expression of hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL), monoacylglycerol lipase (MGL), perilipin, and peroxisome proliferator-activated receptor (PPAR)-γ were measured in each group.Results: In the Ephedra group, body weight, fasting blood sugar, and oral glucose tolerance were significantly decreased. In addition, in the Ephedra group, the gene expression of HSL was significantly increased, whereas that of perilipin was significantly decreased.Conclusions: These results provide evidence that E. sinicapharmacopuncture affects obesity and obesity-induced metabolic syndrome, including insulin resistance and dyslipidemia, by activating lipolysis via the HSL pathway in adipose tissue.

Effects of herbal acupuncture (Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) in obese Rats induced by high fat diet (마황(麻黃), 마황합록다(麻黃合綠茶) 약침(藥鍼)이 고지혈증(高脂血症) 유발(誘發) 백서(白鼠)에 미치는 영향(影響))

  • Youn, Dae-Hwan;Kang, Ja-Don;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.21 no.1
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    • pp.51-60
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    • 2004
  • Objectives : This research was performed to investigate the effect of herbal acupuncture(Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) on weight gain, food intakes, food efficiency ratios, HDL-cholesterol, triglyceride, free fatty acid on rats fed high fat diet for 5weeks. Methods : Experimental groups were divided into normal group(Sham), high fat diet group(Con), high fat diet and Ephedra sinica-herbal acupuncture in SP9 ST40 treated group(ES), high fat diet and Green tea-herbal acupuncture in SP9 ST40 treated group (GT), high fat diet and Ephedra sinica+Green tea-herbal acupuncture in SP9 ST40 treated group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 19.125mg/ml per 2 days during 5 weeks. Results : Body weight, feed intake and feed efficiency ratio were decreased in Sham, ES, ES+GT, compared with control group. The level of serum HDL-cholesterol was increased in sham, tender to increase in ES, ES+GT. The change on that of serum triglyceride was decreased in sham, ES, ES+GT and free fatty acid was decrease in sham, ES+GT. Conclusions : Ephedra sinica with Green tea-herbal acupuncture in SP9 ST40 can control the Body weight, feed intake and feed efficiency ratio, HDL-cholesterol, triglyceride, free fatty acid.

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Effects of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) in obese Rats induced by high fat diet (고지방식이(高脂肪食餌)로 유발(誘發)된 비만백서(肥滿白鼠)모델에서 창출(蒼朮), 의이인(薏苡仁), 마황(麻黃), 창출합의이인(蒼朮合薏苡仁) 마황합록차(麻黃合綠茶) 약침(藥鍼)이 혈청지질(血淸脂質) 및 간기능(肝機能)에 미치는 영향(影響))

  • Youn, Dae-Hwan;Kang, Ja-Don;Joo, Joon-Sung;Chae, Woo-Seok;Na, Chang-Su
    • Journal of Pharmacopuncture
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    • v.7 no.1
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    • pp.87-100
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    • 2004
  • Objective : This research was performed to investigate the effect of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Method : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture group(AJ), high fat diet and Coix lachrymajobi-herbal acupuncture group(CL), high fat diet and Ephedra sinica-herbaI acupuncture group(ES), high fat diet and Atractylodes japonica+Coix lachrymajobi-herbal acupuncture group(AJ+CL), Ephedra sinica+Green tea-herbal acupuncture group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per 2day. Results : Body weight and food efficiency were decreased in AJ, ES, AJ+CL, ES+GT. The level of serum total cholesterol, triglyceride and free fatty acid were increased in AJ, ES, ES+GT. That of serum HDL-cholesterol was increased in AJ. The change of food intake, the level of serum phospholipid and ALP were not significant. The HDL to Total cholesterol ratio was increased in AJ and ES. Conclusion : Atractylodes japonica-herbal acupuncture in ST40 SP9 is effective on Body weight, food efficiency ratio, the level of serum lipid, protection of liver function and prevention cardiovascular risk by obesity induced by high fat diet. Herbal acupuncture mixed Ephedra sinica with Green tea can control the body weight, food efficiency ratio and the level of serum lipid.

Analysis of the Anti-Allergic Activities of Active Components Produced by Solid Fermentation of Phellinus baumii and Ephedra sinica (장수상황버섯과 마황을 이용한 고체발효 추출물로부터 항아토피 활성의 분석)

  • Shin, Yong-Kyu;Heo, Jin-Chul;Lee, Jin-Hyung;Lee, Sang-Han
    • Korean Journal of Food Preservation
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    • v.17 no.2
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    • pp.297-300
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    • 2010
  • To evaluate whether active components produced by solid fermentation of Phellinus baumii and Ephedra sinica have potential in ameliorating allergic symptoms in mice, we tested anti-allergic activities in a dinitrofluorobenzene (DNFB)-induced allergic mouse model. DNFB-induced allergic symptoms werereduced to about 50% of control levels by active components produced by solid fermentation of Phellinus baumii and Ephedra sinica, as evaluated by measuring the width of epidermal swelling. H&E staining also revealed that these active components markedly reduced allergic symptoms in the epidermis of the ear. The results indicate that active components produced by solid fermentation of Phellinus baumii and Ephedra sinica have the potential to ameliorateallergic symptoms, and may be useful biomaterial(s) in the neutraceutical or cosmeceutical industry.

Effect of Herbal Medicines on the IgA Production in Peyer patches (Peyer patch 세포에서 임상 빈용생약이 IgA 생산성에 미치는 영향)

  • Bae, Eun-Ah;Han, Myung-Joo;Kim, Dong-Hyun
    • Korean Journal of Pharmacognosy
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    • v.31 no.3
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    • pp.259-263
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    • 2000
  • This study was designed to evalulate the biological activity, particularly induction of IgA in primary Peyer's patches cells, of herbal medicines for a long time in Asia. Among fifty four herbal medicines tested, Ephedra sinica, Magnolia officinalis, Lonicera japonica and Lithospermum erythorhizon induced IgA production. The polar and high molecular weight component in Ephedra sinica was capable of inducing IgA in primary Peyer's patches cells. Therefore, we suggest that some herbal medicines could use for the treatment of food allergy.

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Inhibitory Effects of Eugenia caryophyllate, Ephedra sinica and Cinnamomum cassia on the Replication of HBV in HepG2 2.2.15 Cells (정향, 마황, 계피의 간염 B형 바이러스 증식 억제 효과)

  • 강석연;김태균;박민수;한형미;정기경;강주혜;문애리;김승희
    • Biomolecules & Therapeutics
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    • v.7 no.2
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    • pp.133-137
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    • 1999
  • This study was undertaken to test for anti-Hepatitis B virus (HBV) activity of the aqueous extracts prepared from Eugenia caryophyllate, Ephedra sinica, Cinnamomum cassia. Aqueous extracts were assayed for the inhibition of HBV replication by measurement of HBV DNA and surface antigen (HBsAg) levels in the extracellular medium of HePG2 2.2.15 cells. All extracts decreased the levels of extracellular HBV virion DNA at concentrations ranging from 128 to 256 $\mu$g/ml and inhibited the production of HBsAg dose-dependently. Our findings suggest that these three hebal medicinal plants may have potential to develop as specific anti-HBV drugs in the future.

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