• Title, Summary, Keyword: Escherichia coli

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Portulaca oleracea L. 추출물에 의한 Escherichia coli KCTC 2441의 생육억제

  • Lee, Eun-Sook;Seo, Bu-Il
    • Herbal Formula Science
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    • v.9 no.1
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    • pp.367-374
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    • 2001
  • This study was performed to investigate the effects of purslane extracts on growth inhibition of Escherichia coli KCTC 2441. The purslane extract is effective on retarding growth of Escherichia coli KCTC 2441 and effect of its extract was founded from 1% concentration. At 7% the growth of Escherichia coli KCTC 2441 was suddenly retarded. The stem of purslane in a sunny place on June is more effective than its leaf. Leaves with a red violet color has high effect on retarding growth of Escherichia coli KCTC 2441. These results suggest that purslane extract is effective in growth inhibition of Escherichia coli KCTC 2441.

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Optimized Culture Conditions for Production of the chimaeric protein, Uropathogenic Escherichia coli Adhesin - Cholera Toxin A2B Subunits, in Escherichia coli TB1

  • Lee, Yong-Hwa;Kim, Byung-Oh;Rhee, Dong-Kwon;Pyo, Suh-Kneung
    • Biomolecules & Therapeutics
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    • v.12 no.3
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    • pp.179-184
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    • 2004
  • The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause for urinary tract infections. In our previous study, the Adhesin/CTXA2B was expressed as soluble recombinant chimaeric protein derived from the uropathogenic Escherichia coli adhesin genetically coupled to cholera toxin A2B (CTXA2B) subunit in Escherichia coli. Since it is very important to optimize IPTG concentration and culture temperature to maximize cell growth and productivity, These optimal culture factors were determined to increase the productivity of the expressed Adhesin/CTXA2B chimaeric protein in Escherichia coli TB1 carrying pMALfimH/ctxa2b. Our data demonstrate that optimal concentration of IPTG for increased production of chimaeric protein was 0.5 mM. Additionally, culture time was 10 hours and temperature, 37${\circ}C$.

Optimal culture conditions for production of Escherichia coli Adhesin protein coupled to Escherichia coli Heat Labile Enterotoxin A2B in Escherichia coli TB1.

  • Lee, Yong-Hwa;Rhee, Dong-Kwon;Pyo, Suhk-Neung
    • Proceedings of the PSK Conference
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    • pp.226.2-226
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    • 2003
  • The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause of urinary tract infection. To produce a possible vaccine antigen against urinary tract infection, the fimH gene was genetically linked to the Itxa2b gene, which was then cloned into the pMAL -p2E expression vector. The chimaeric construction of pMALfimH/Itxa2b was transformed into Escherichia coli TB1 and its N-terminal amino acid sequence was analyzed. (omitted)

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Removal characteristic of Escherichia coli for Discharge tube with globular $SiO_2$ (구형 $SiO_2$를 갖는 방전관의 수중 세균제거특성)

  • Lee, Dong-Hoon;Park, Hong-Jae;Park, Jae-Youn
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • pp.546-549
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    • 2003
  • This paper was shown removal characteristic of escherichia coli for discharge tube with globular $SiO_2$. At the result of the removal characteristic experiments of escherichia coli using the discharge tube with globular $SiO_2$, because the electric field is also increased when input voltage is increased, the removal characteristic of escherichia coli was appeared relation connection to input voltage. If a passing number of test water in discharge tube is increased, the removal ratio of escherichia coli is increased because passing number of electric field section is increased. When diameter of globular $SiO_2$ is increased, the removal time of escherichia coli is to be decreased because dielectric polalization of globular dielectric($SiO_2$), Also, the removal ratio of escherichia coli of the discharge tube with globular dielectric($SiO_2$) is appeared higher than the removal ratio of the discharge tube without globular dielectric($SiO_2$).

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Property Conversion of Water and Removing Characteristics of Escherichia Coli Inner Discharge Tube With ZrO$_2$Beads (구형 ZrO$_2$를 충진한 방전관의 수질 변환 및 대장균제거 특성)

  • 이동훈;박재윤
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.53 no.3
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    • pp.143-148
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    • 2004
  • This paper shows property conversion of water and removing characteristics of Escherichia coli for discharge tube with $ZrO_2$ beads. At the result of the removal characteristic experiments of Escherichia coli using the discharge tube with $ZrO_2$ beads, because the electric field is also increased when input voltage is increased, the removal characteristic of Escherichia coli was appeared relation connection to input voltage. And if a passing number of test water in discharge tube with $ZrO_2$beads is increased, the removal ratio of Escherichia coli is to be increased because passing number of electric field section is increased. And if diameter of $ZrO_2$beads is increased, the removal time of Escherichia coli is to be decreased because dielectric polalization of $ZrO_2$beads. Also, the removal ratio of Escherichia coli of the discharge tube with $ZrO_2$beads. is appeared higher than the removal ratio of the discharge tube without $ZrO_2$beads. And a satulation punt of ozone and $H_2$ $O_2$generation density inner water was appeared near 60[min].

Electric Field Analysis and Removal Characteristics of Escherichia Coli for Water Discharge Tube with Globular SiO$_2$ (구형 SiO$_2$를 갖는 수방전관의 전계 해석 및 대장균 제거 특성)

  • 이동훈
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.53 no.2
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    • pp.103-108
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    • 2004
  • This paper shows the simulation of electric field distribution and removal characteristics of Escherichia coli for water discharge tube with globular $SiO_2$. At the experiments of the removing Escherichia coli used the discharge tube with globular dielectric($SiO_2$), because the electric field is increased when applied voltage is increased, the removed ratio of Escherichia coli was related with increasing of applied voltage. When a passing number of test water in water discharge tube is increased, the removed ratio of Escherichia coli is increased because passing number of territory with electric field is increased. When diameter of globular dielectric($SiO_2$) is increased, the removed time of Escherichia coli was decreased because electric field for dielectric polarization of globular dielectric($SiO_2$) was increased. Also, the removed ratio of Escherichia coli of the water discharge tube with globular dielectric($SiO_2$) was measured higher than the removal ratio of the discharge tube without globular dielectric($SiO_2$)

Habituation of Escherichia coli O157:H7 in Alkali and Susceptibility of Causative Agents (알칼리조건에서 Escherichia coli 0157:H7의 적응 및 항균요법제에 대한 감수성)

  • 배종호
    • The Korean Journal of Food And Nutrition
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    • v.12 no.6
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    • pp.564-568
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    • 1999
  • Habituation to alkali condition and susceptibility to antimicrobial agents were investigated to determine methods t inactivation Escherichia coli O157:H7 associated with food poisoning. The investigation showed that Escherichia coli O157:H7growth at pH 9.0 The susceptibilities of Escherichia coli O157:H7 to 13 drugs examined were found highest in regad to saicin and ceftriaxone and lowest in regard to erythromycin and josamycin. No significant changes in minimal inhibitory concen-tration(MIC) values of bactericidal effect was found when saicin and ceftriaxone were irradiated with doses of 5-100KGy.

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Cloning of Bacillus amyloliquefaciens amylase gene using YRp7 as a vector I. Expression of cloned amylase gene in Escherichia coli (YRp 7 vector를 이용한 Bacillus amyloliquefaciens amylase gene의 cloning I. Escherichia coli에서의 발현)

  • 서정훈;김영호;전도연;홍순덕;조윤래
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.161-168
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    • 1986
  • A 1.95Kb Sau3Al fragment coding for $\alpha$-amylase from Bacillus amyloliquefaciens was isolated by the shotgun method using Escherichia coli as a host. The genome of Bacillus amyloliquefaciens was partially digested with the restriction endonuclease Sau3Al and joined to plasmid YRp7 cleaved with the restriction endonuclease BamHI. The $\alpha$-amylase gene present in a 1.95Kb insert was stably maintained and expressed in Escherichia coli. The amount of $\alpha$-amylase activity produced by Escherichia coli containing the hybrid plasmid pEA24 was about 65% of the activity produced by the donor Bacillus amyloliquefaciens strain. The properties of $\alpha$-amylase produced by Escherichia coli were very similar to those produced by Bacillus amyloliquefaciens as based on optimum temperature, pH, and effect of CaCl$_2$ concentration. About 70% of the $\alpha$-amylase produced by Escherichia coli was localized in the periplasmic space, whereas the remaining enzyme was localized in the inner part of the cell.

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Studies on pathogenicity of Escherichia coli isolated from chickens: Correlation between in vitro Congo-red binding properties and in vivo virulence in avian Escherichia coli (닭유래 Escherichia coli의 병원성에 관한 연구 : 시험관내 Congo-red 결합능과 병원성간의 상관관계)

  • Woo, Yong Ku;Kim, Ki Seuk;Kim, Bong Hwan
    • Korean Journal of Veterinary Research
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    • v.31 no.1
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    • pp.55-61
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    • 1991
  • The correlation between in vitro Congo-red binding properties of E coli and in vivo invasiveness of the organisms in SPF chickens and mice was investigated. Congo-red positive E coli colonies were dark-red color with a typical colonial morphology of rough appearance when grown on Congo-red medium, while Congo-red negative colonies showed pale-pink color and smooth surfaced colonial morphology. Pathogenicity of 10 Congo-red positive E coli for mice was observed in 92.5% but that of 5 Congo-red negative E coli was 45%. Invasiveness of 10 Congo-red positive E coli for chickens was observed in 96% of the SPF chickens tested but that of 5 Congo-red negative E coli was 16% only. These results of pathogenicity studies with E coli isolates indicate a significant correlation between Congo-red binding ability and virulence in avian Escherichia coli.

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Removal characteristic of Escherichiacoli and water property conversion for Discharge tube with globular $ZrO_2$ (구형 지르코니아를 갖는 수방전관의 수질성분변환 및 세균제거특성)

  • Lee, Dong-Hoon;Park, Hong-Jae;Park, Jae-Youn
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • pp.550-553
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    • 2003
  • This paper was shown water properly conversion and removal characteristic of escherichia coli for discharge tube with $ZrO_2$ beads. At the result of the removal characteristic experiments of escherichia coli using the discharge tube with $ZrO_2$ beads, because the electric field is also increased when input voltage is increased, the removal characteristic of escherichia coli was appeared relation connection to input voltage. And if a passing number of test water in discharge tube with $ZrO_2$ beads is increased, the removal ratio of escherichia coli is to be increased because passing number of electric field section is increased. And if diameter of $ZrO_2$ beads is increased, the removal time of escherichia coli is to be decreased because dielectric polalization of $ZrO_2$ beads. Also, the removal ratio of escherichia coli of the discharge tube with $ZrO_2$ beads. is appeared higher than the removal ratio of the discharge tube without $ZrO_2$ beads. And a salutation point of ozone and $H_2O_2$ generation density inner water was appeared near 60[min].

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