• Title, Summary, Keyword: Essential oils

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Chemical Constituents of Essential Oils Possessing Anti-Influenza A/WS/33 Virus Activity

  • Choi, Hwa-Jung
    • Osong Public Health and Research Perspectives
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    • v.9 no.6
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    • pp.348-353
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    • 2018
  • Objectives: This study was conducted to determine whether essential oils had anti-influenza A/WS/33 virus activity and whether there were specific compounds associated with this activity. Methods: There were 63 essential oils evaluated for anti-influenza (A/WS/33 virus) activity using a cytopathic effect reduction method. The chemical composition of the anti-influenza essential oils was phytochemically analyzed by gas chromatography-mass spectrometry. Results: The antiviral assays demonstrated that 11 of the 62 essential oils ($100{\mu}g/mL$) possessed anti-influenza activity, reducing visible cytopathic effects of influenza A/WS/33 virus activity by > 30%. Furthermore, marjoram, clary sage and anise oils exhibited anti-influenza A/WS/33 virus activity of > 52.8%. However, oseltamivir (the anti-influenza A and B drug), showed cytotoxicity at the same concentration ($100{\mu}g/mL$) as the essential oils. The chemical composition detected by GC-MS analysis, differed amongst the 3 most potent anti-viral essential oils (marjoram, clary sage and anise oils) except for linalool, which was detected in all 3 essential oils. Conclusion: This study demonstrated anti-influenza activity in 11 essential oils tested, with marjoram, clary sage and anise essential oils being the most effective at reducing visible cytopathic effects of the A/WS/33 virus. All 3 oils contained linalool, suggesting that this may have anti-influenza activity. Further investigation is needed to characterize the antiviral activity of linalool against influenza A/WS/33 virus.

Analysis of Essential Oils from Elscholtzia ciliata and the Production of Essential Oils by Tissue Culture (향유의 정유 분석 및 조직배양)

  • Chi, Hyung-Joon;Shin, Soon-Hee;Chang, Jung-In
    • Korean Journal of Pharmacognosy
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    • v.23 no.2
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    • pp.77-80
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    • 1992
  • The essential oils of Elscholtzia ciliata and the cultivated tissue have been studied. The composition and contents of essential oils were identified by gas chromatography and mass spectrometry. The main component of essential oils of E. ciliata was naginata ketone. The essential oils from the flowers, leaves and stems of E. ciliata showed similar patterns of gas chromatogram. In experimental studies on the tissue culture of callus, it has been found that NAA induced higher growth rate and higher content of essential oils than 2, 4-D. The essential oils from the cultivated callus showed different composition from that of mother plants.

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Activity of Essential Oils Against Bacillus subtilis Spores

  • Lawrence, Hayley A.;Palombo, Enzo A.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1590-1595
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    • 2009
  • Alternative methods for controlling bacterial endospore contamination are desired in a range of industries and applications. Attention has recently turned to natural products, such as essential oils, which have sporicidal activity. In this study, a selection of essential oils was investigated to identify those with activity against Bacillus subtilis spores. Spores were exposed to 13 essential oils, and surviving spores were enumerated. Cardamom, tea tree, and juniper leaf oils were the most effective, reducing the number of viable spores by 3 logs at concentrations above 1%. Sporicidal activity was enhanced at high temperatures ($60^{\circ}C$) or longer exposure times (up to 1 week). Gas chromatography-mass spectrometry analysis identified the components of the active essential oils. However, none of the major oil components exhibited equivalent activity to the whole oils. The fact that oil components, either alone or in combination, did not show the same level of sporicidal activity as the complete oils suggested that minor components may be involved, or that these act synergistically with major components. Scanning electron microscopy was used to examine spores after exposure to essential oils and suggested that leakage of spore contents was the likely mode of sporicidal action. Our data have shown that essential oils exert sporicidal activity and may be useful in applications where bacterial spore reduction is desired.

A Study on the Medical Care System using Essential Oil (메디컬피부케어를 위한 에센셜 오일 적용방법에 대한연구)

  • Lee, Ae-Sun
    • Journal of the Korean Society of Fashion and Beauty
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    • v.5 no.3
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    • pp.111-117
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    • 2007
  • Essential oils aims to rejuvenate and beautify medical skin care the body through the use of essential oils, and generally to improve our outward appearance. Much of this section fouses on the different methods by which essential oils can be used to improve the complexion or to treat specific medical care skin conditions. Beauty, however, is not just skin-deep. The condition of the medical care express the overall health of an individual. A relaxed attitude, together with a well-balanced diet, enough exercise, and a daily intake of plenty of spring water or herbal teas, all help to keep the system in top condition. Essential oils is a healing art which has powerful effects on both mind and body. The vital element in essential oils is the pure oils which are extracted from various part of different plants. The key to medical aromatherapy care lies in its dual use of essential oils and medical care massage, thus masking full use of two of our most important senses-smell and touch. Medical aromatherapy care is holistic, working mainly on the nervous system and including the head and body as part of the same treatment. Using pressure points along the spine, Medical aromatherapy care on the autonomic nervous system and has an immediate effect of relexation. In directly it also acts on mood. Many of the medical massage movements help lymphatic drainage. Essential oils have many different qualities; they can be relaxing or invigorating and are generally antisepic and antibacterial. Medical aromatherapy care can have psychological benefits. The power of essences in healing has been recognized in the treatment of insect bites. Clove, thyme, sandalwood, and lavender are a few of the essences that have antiseptic. Essential oils effect the dry skin for small visual pores, dull matte finish, rough sandy feeling, tantskin. Essential oils effect the mature/sun damaged skin for some red or couperose areas, loose saggy skin, exposure to a lot of ultraviolet light. Essential oils effect the medical care nomal skin good elasticity, healthy color(good circulation), smooth terture.

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In Vitro and In Vivo Anti-Tobacco Mosaic Virus Activities of Essential Oils and Individual Compounds

  • Lu, Min;Han, Zhiqiang;Xu, Yun;Yao, Lei
    • Journal of Microbiology and Biotechnology
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    • v.23 no.6
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    • pp.771-778
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    • 2013
  • Essential oils are increasingly of interest for use as novel drugs acting as antimicrobial and antiviral agents. In the present study, we report the in vitro antiviral activities of 29 essential oils, extracted from Chinese indigenous aromatic plants, against the tobacco mosaic virus (TMV). Of these essential oils, those oils from ginger, lemon, tea tree, tangerine peel, artemisia, and lemongrass effected a more than 50% inhibition of TMV at 100 ${\mu}g/ml$. In addition, the mode of antiviral action of the active essential oils was also determined. Essential oils isolated from artemisia and lemongrass possessed potent inactivation and curative effects in vivo and had a directly passivating effect on TMV infection in a dose-dependent manner. However, all other active essential oils exhibited a moderate protective effect in vivo. The chemical constitutions of the essential oils from ginger, lemon, tea tree, tangerine peel, artemisia, and lemongrass were identified by gas chromatography and gas chromatography-mass spectrometry. The major components of these essential oils were ${\alpha}$-zingiberene (35.21%), limonene (76.25%), terpinen-4-ol (41.20%), limonene (80.95%), 1,8-cineole (27.45%), and terpinolene (10.67%). The curative effects of 10 individual compounds from the active essential oils on TMV infection were also examined in vivo. The compounds from citronellal, limonene, 1,8-cineole, and ${\alpha}$-zingiberene effected a more than 40% inhibition rate for TMV infection, and the other compounds demonstrated moderate activities at 320 ${\mu}g/ml$ in vivo. There results indicate that the essential oils isolated from artemisia and lemongrass, and the individual compound citronellal, have the potential to be used as an effective alternative for the treatment of tobacco plants infected with TMV under greenhouse conditions.

Antifungal Activity and Inhibitory Modes of Volatile Vapours of Essential Oils

  • Chee, Hee-Youn;Lee, Eun-Hee
    • Mycobiology
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    • v.32 no.2
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    • pp.102-104
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    • 2004
  • Antifungal activities of volatile vapours of essential oils were investigated. Volatile vapours of test essential oils except Cedar-wood and Pachouri showed inhibitory activity against test fungi. Volatile vapours of Birch essential oils exhibited fungistatic activity against dermatophytic filamentous fungi while others did fungicidal activity. Spores of dermatophytic filamentous fungi are more susceptible to volatile vapours of essential oils than mycelia.

Antimicrobial Activity of Essential Oil against Oral Strain

  • Park, Chungmu;Yoon, Hyunseo
    • International Journal of Clinical Preventive Dentistry
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    • v.14 no.4
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    • pp.216-221
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    • 2018
  • Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouthwash, and other uses. Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, $20{\mu}l$ of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times. Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity. Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.

Classification of Aroma Essential Oils by Sasang Constitution (아로마 에센셜 오일의 사상체질의학적 분류제목)

  • Choi, Eun-Joo;Hong, Sun-Gi;Yoo, Jun-Sang
    • Journal of Sasang Constitutional Medicine
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    • v.23 no.3
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    • pp.304-317
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    • 2011
  • 1. Objectives: Aromatherapy is one of the alternative medical treatments by flavors of essential oils extracted from plants. This study was to investigate the classification of aroma essential oils by Sasang Constitution for clinical applications. 2. Methods: 1) We searched scientific name of aroma essential oils and scientific name of herbs, classify aroma essential oils according to Sasang Constitution. 2) If the method 1) is impossible, sort them by regard of aroma essential oils' flavors, specific characters and efficacies. 3. Results: Aroma essential oils suitable for Soeumin are 27 kinds, for Soyangin and Taeeumin are each 16 kinds, and for Taeyangin are 5 kinds.(Duplicates are allowed.) The cases which can not be classified clearly, are 10 kinds, so they are able to consider the Constitution of the more than two-phase. 4. Conclusions: Most of aromatic herbs are warm and spicy by pre-reports. So, aroma essential oils suitable for Soeumin are most often. After this study, studies about Sasang Constitutional herb recipes and more clinical studies are needed.

Antibacterial Activity of Essential Oils on the Growth of Staphylococcus aureus and Measurement of their Binding Interaction Using Optical Biosensor

  • Chung, Kyong-Hwan;Yang, Ki-Sook;Kim, Jin;Kim, Jin-Chul;Lee, Ki-Young
    • Journal of Microbiology and Biotechnology
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    • v.17 no.11
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    • pp.1848-1855
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    • 2007
  • Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree>Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were $5.0{\times}10^{-13}\;ml/(CFU{\cdot}s)$ and $5.0{\times}10^5\;ml/CFU$, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.

Effects of Citrus Essential Oils on Melanin Production in B16 Melanoma Cells (B16 Melanoma 세포에서 Citrus Essential Oil이 Melanin 생성에 미치는 영향)

  • 임혜원;조남영;윤미연;차상복;김경원;박영미;이지윤;이진희;김창종
    • YAKHAK HOEJI
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    • v.47 no.1
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    • pp.25-30
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    • 2003
  • This study is performed to investigate the effects of citrus essential oils on melanin production in B16 melanoma cells. Five kinds of citrus essential oils (Bergamot, Grapefruit, Lemon, Mandarin, Petigrain) did not have any influence on DPPH radical scavenger activity, cell growth and cytotoxicity in B16 melanoma cells. Both mandarin and petigrain essential oils dose-dependently inhibited purified tyrosinase activity, but bergamot did not. In 1$\mu$M MSH-stimulated B16 melanoma cells, all of 5 citrus essential oils inhibited melanin production in a dose dependent manner. From the above results, it is possible that citrus essential oils may be developed to be an anti-melanogenesis agent on the basis of their inhibitory effect on MSH-induced melanin production.