• Title/Summary/Keyword: Experimental Infection

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Experimental infection of Edwardsiella tarda in the Tilapia (틸라피아에 있어서 Edwardsiella tarda의 실험적 감염에 관한 연구)

  • Kim, Kwang-Hee;Choi, Dong-Lim;Chung, Joon-Ki;Chun, Seh-Kyu
    • Journal of fish pathology
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    • v.5 no.2
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    • pp.61-75
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    • 1992
  • Experimental infection method for Edwardsiellosis was studied to imitate histopathologically natural infections in the Tilapia Oreochrimis niloticus. Prior to the bacterial challenge, the intestine of tilapia was damaged by 0.2ml of 30% hydrogen peroxide introduced through a silicon tube which was inserted 4 to 5cm into the intestine from anus. E. tarda was mixed a 10% Gum arabia and administered into the stomach by a cannula 20 hours after the hydrogen peroxide treatment. Bacterial doses used were from $4.2{\times}10^6$ to $6.4{\times}10^\;CFU$/fish. Fish 72 hours after the challenged showed swollen and erosious lesion and focal necrosis with bacterial-eaden inflammatory cell in the Liver, Spleen, Kidney. Fish 96 and 120 hours after bacterial challenge were moribund showing swollen Liver, Spleen, Kidney and focal accumulation of macrophages and production of granulomas in the infected lesions.

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A Case of Listeriosis Occurred in the Korean Native Goat (한국재래산양에서 발생한 리스테리아병)

  • Yeo, Sang-geon;Kim, Soon-bok;Choe, Sang-yong
    • Korean Journal of Veterinary Research
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    • v.27 no.2
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    • pp.207-213
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    • 1987
  • A survey was performed on the outbreak of listeriosis in a Korean native goat in Animal Breeding Station of Gyeongsang National University, Chinju, Korea. Clinical signs in the affected goat were fever, dullness, inappetence, nasal discharge, slobbering, dysphagia, circling, incoordination, recumbency, unilateral facial paralysis, torticollis, dyspnea, spasmodic paddling movement of the limbs and death at 6 days after onset. No significant gross lesions were seen. The histopathological lesions were consisted of perivascular edema and cuffs by round cells and microabscess with infiltration of neutrophils, lymphocytes and macrophages in the cerebrum. Gram-positive organism was observed in the microabscess lesions. Listeria monocytogenes was isolated exclusively from the brain tissue by cultural examination and the lesions induced by experimental infection with the isolate were characterized by severe conjunctivitis in rabbit and hepatic necrotic foci in mouse. This seems to be the first report of listeriosis in Korean native goat.

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ACUTE INFECTIOUS BURSAL DISEASE IN CHICKENS : PATHOLOGICAL OBSERVATION AND VIRUS ISOLATION

  • Chowdhury, E.H.;Islam, M.R.;Das, P.M.;Dewan, M.L.;Khan, M.S.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.4
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    • pp.465-469
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    • 1996
  • Pathological and virological investigations were conducted on suspected outbreaks of infectious bursal disease (IBD) in a broiler farm and five pullet-raising poultry farms of Mymensingh and Tangail districts of Bangladesh. About 80 to 100 percent chicks were affected at the age of 26 to 45 days and mortality varied from 20 to 30 percent in broilers and 40 to 80 percent in layer chicks. Signs, symptoms, gross and microscopic lesions were typical of acute IBD. Several isolates of virus could be obtained by embryo inoculation and the virus was diagnosed as infectious bursal disease virus (IBDV) by agar gel immunodiffusion test (AGID). The virus isolate belonged to the very virulent pathotype of IBDV causing 100 percent mortality in three weeks old chicks on experimental infection.

Experimental infection of white spot syndrome virus (WSSV) in wild crab, Gaetice depressus

  • Kim, Chun Soo;Kim, Ki Hong
    • Journal of fish pathology
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    • v.17 no.3
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    • pp.213-216
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    • 2004
  • To confirm the possibility of a wild marine crab, Gaetice depressus, as a carrier for white spot syndrome virus (WSSV) and to develop an alternative experimental model for WSSV in winter season, the susceptibility of the crab to WSSV was assessed by artificial challenge and subsequently tested for infection by PCR assay. The results revealed that the crabs were as highly susceptible as penaeid shrimps. WSSV caused 100% mortality in G. depressus within 16 days after intramuscular injection. The presence of WSSV in the moribund crabs was confirmed by PCR and was found in gills and muscle tissue. These results suggest that G. depressus can be naturally infected by WSSV via moribund shrimps, and can act as a potential carrier of WSSV. In addition, G. depressus can be used as an alternative experimental animal for WSSV.

Experimental human infection with Fibricola cratera (Trematoda: Neodiplostomidae)

  • Shoop, Wesley-L.
    • The Korean Journal of Parasitology
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    • v.27 no.4
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    • pp.249-252
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    • 1989
  • Fibricola cratera is a strigeoid trematode indigenous to North America that, heretofore, was known only to infect wild mammals. Herein, it is reported that an experimental inoculation of a human volunteer produced a patellt infection that lasted 40 months. Symptoms of epigastric discomfort, loose stools and flatulence occurred over the first year of infection and ameliorated thereafter. Eggs per gram of stool were low (${\leq}2$) throughout the course of infection and were not detected by the standard technique of formalin-ether concentration. To monitor infection, the entire stool sample was examined each month after sieving through No. 10 (pore size 2 mm) and 100 (pore size $145{\;}{\mu\textrm{m}}$) sieves and collecting eggs on a No. 325 (pore size $45{\;}{\mu\textrm{m}}$) sieve. This is the first report of a North American strigeoid trematode capable of maturing in a human and is only the second species of strigeoid known to do so. The other species is F. seoulensis which has been implicated in 26 human infections in Korea.

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Isolation of Photobacterium damselae subsp. damselae from zebra shark Stegostoma fasciatum

  • Han, Jee Eun;Gomez, Dennis K.;Kim, Ji Hyung;Choresca, Casiano H. Jr.;Shin, Sang Phil;Baeck, Gun Wook;Park, Se Chang
    • Korean Journal of Veterinary Research
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    • v.49 no.1
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    • pp.35-38
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    • 2009
  • The zebra shark Stegostoma fasciatum which had been reared in the commercial aquaria was found dead and submitted for postmortem examination. A pure bacterial culture was isolated from pale and enlarged liver. The analysis of ureC and 16S rRNA genes confirmed the isolate as Photobacterium (P.) damselae subsp. damselae and this pathogen was sensitive to gentamicin. Although, no mortality in mouse was observed in the experimental infection study, the isolation of this pathogen in aquarium fish is significant because it can act as a reservoir to other aquatic animals and can also be zoonotic potential to human during aquarium management. This paper describes the first isolation of P. damselae subsp. damselae from zebra shark.

Changes of Serum Adiponectin Levels in Murine Experimental Sparganosis

  • Yang, Hyun-Jong
    • The Korean Journal of Parasitology
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    • v.46 no.2
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    • pp.91-93
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    • 2008
  • The weight gain phenomenon associated with sparganosis has been well documented and was first recognized in the 1960s. Many studies have been conducted regarding the plerocercoid growth factor in the larva of Spirometra mansoni. In the present study, we hypothesized that the weight gain may be affected by the adipocyte secreted hormones, i.e., adiponectin, which is secreted from the adipose tissues in case of tissue migrating parasitic infections. Specifically, we attempted to ascertain whether the serum levels of adiponectin change in murine sparganosis. However, serum adiponectin levels assayed by ELISA evidenced no significant changes after an experimental infection (P > 0.05). Finally, the weight gain phenomenon in mouse sparganosis is not associated with changes in adiponectin levels, and further investigations involving parasitic infection-induced weight gain remain necessary.

Expression of TNF-${\alpha}$ and IL-$1{\beta}$ in Splenic Dendritic Cells and Their Serum Levels in Mouse Sparganosis

  • Yang, Hyun-Jong
    • The Korean Journal of Parasitology
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    • v.49 no.2
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    • pp.191-194
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    • 2011
  • Sparganosis is a tissue invading helminthiasis infecting intermediate hosts, including humans. Strong immune responses are expected to occur in early phases of infection. Thus, we investigated cytokine expressions in splenic dendritic cells and in sera after experimental infection of mice. In splenic dendritic cells, TNF-${\alpha}$ and IL-$1{\beta}$ expression peaked at week 1 and week 3 post -infection (PI), respectively, and also early phase (week 2 PI) depressed cytokine expression was noticed. Serum IL-$1{\beta}$ concentration increased significantly at week 2 PI and peaked at week 6 PI, and that of TNF-${\alpha}$ peaked at week 6 PI. These results showed that pro-inflammatory cytokines, TNF-${\alpha}$ and IL-$1{\beta}$, are chronologically regulated in mouse sparganosis.

Experimental Toxocara catiInfection in Gerbils and Rats

  • Zibaei, Mohammad;Sadjjadi, Seyed Mahmoud;Uga, Shoji
    • The Korean Journal of Parasitology
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    • v.48 no.4
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    • pp.331-333
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    • 2010
  • Mongolian gerbils and Wistar rats were inoculated orally with 240 and 2,500 Toxocara cati embryonated eggs, respectively, to evaluate the larval recovery in different tissues and organs, such as the liver, lungs, heart, kidney, and skeletal muscles after 5, 30, 49, 70, and 92 days post-infection (PI). Larval recovery rates were 1.7-30.0% in Mongolian gerbils on days 5-92 PI and 0.2-3.8% in rats on the same days. These results indicate that Mongolian gerbils and Wi star rats are suitable experimental paratenic hosts for the study of neurological toxocariasis as well as visceral toxocariasis.

Experimental Infection with Cheolwon/Incheon Isolates of Classical Swine Fever Virus in Pig: Clinical Signs and Pathology

  • Kim, Jae-hoon;Kang, Kyung-il;Roh, In-soon;Kim, Heui-jin;Jean, Young-hwa;Cha, Sang-ho;Park, Eun-jin;Yoon, So-rah;Seok, Ju-myung;Song, Jae-Young;Kweon, Jun-Heon
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • pp.12-12
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    • 2003
  • Several cases of classical swine fever (CSF) were reoccurred in Cheolwon province and Incheon city in 2002. Two isolates (Cheolwon and Incheon) of classical swine fever viruses (CSFV) were successfully isolated and classified into genotype-2 with a phylogenetic analysis [l, 2]. (omitted)

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