• Title, Summary, Keyword: Foetus

Search Result 15, Processing Time 0.1 seconds

Efficacy of Ronidazole for Treatment of Cats Experimentally Infected with a Korean Isolate of Tritrichomonas foetus

  • Lim, Sun;Park, Sang-Ik;Ahn, Kyu-Sung;Oh, Dae-Sung;Shin, Sung-Shik
    • The Korean Journal of Parasitology
    • /
    • v.50 no.2
    • /
    • pp.161-164
    • /
    • 2012
  • To evaluate the efficacy of ronidazole for treatment of Tritrichomonas foetus infection, 6 Tritrichomonas-free kittens were experimentally infected with a Korean isolate of T. foetus. The experimental infection was confirmed by direct microscopy, culture, and single-tube nested PCR, and all cats demonstrated trophozoites of T. foetus by day 20 post-infection in the feces. From day 30 after the experimentally induced infection, 3 cats were treated with ronidazole (50 mg/kg twice a day for 14 days) and 3 other cats received placebo. Feces from each cat were tested for the presence of T. foetus by direct smear and culture of rectal swab samples using modified Diamond's medium once a week for 4 weeks. To confirm the culture results, the presence of T. foetus rRNA gene was determined by single-tube nested PCR assay. All 3 cats in the treatment group receiving ronidazole showed negative results for T. foetus infection during 2 weeks of treatment and 4 weeks follow-up by all detection methods used in this study. In contrast, rectal swab samples from cats in the control group were positive for T. foetus continuously throughout the study. The present study indicates that ronidazole is also effective to treat cats infected experimentally with a Korean isolate of T. foetus at a dose of 50 mg/kg twice a day for 14 days.

First Report of Feline Intestinal Trichomoniasis Caused by Tritrichomonas foetus in Korea

  • Lim, Sun;Park, Sang-Ik;Ahn, Kyu-Sung;Oh, Dae-Sung;Ryu, Jae-Sook;Shin, Sung-Shik
    • The Korean Journal of Parasitology
    • /
    • v.48 no.3
    • /
    • pp.247-251
    • /
    • 2010
  • Feline intestinal tritrichomoniasis by Tritrichomonas foetus was first recognized in USA in 1999 and has so far been reported from UK, Norway, Switzerland, and Australia, but not from the Far East Asian countries. In November 2008, 2 female and male littermate Siamese cats, 6-month old, raised in a household in Korea were referred from a local veterinary clinic with a history of chronic persistent diarrhea. A direct smear examination of fecal specimens revealed numerous trichomonad trophozoites which were isolated by the fecal culture in $InPouch^{TM}$ TF-Feline medium. A PCR testing of the isolate based on the amplification of a conserved portion of the T. foetus internal transcribed spacer (ITS) regions (ITS1 and ITS2) and the 5.8S rRNA gene, and the molecular sequencing of the PCR amplicons confirmed infection with T. foetus. This is the first clinical case of feline intestinal trichomoniasis caused by T. foetus in Korea.

Prevalence and Molecular Characterization of Intestinal Trichomonads in Pet Dogs in East China

  • Li, Wen-Chao;Wang, Kai;Zhang, Wei;Wu, Jingjing;Gu, You-Fang;Zhang, Xi-Chen
    • The Korean Journal of Parasitology
    • /
    • v.54 no.6
    • /
    • pp.703-710
    • /
    • 2016
  • The trichomonad species Tritrichomonas foetus and Pentatrichomonas hominis were recently detected in the feces of dogs with diarrhea. However, little information is available on the prevalence and pathogenicity of these parasites in the canine population. Therefore, the aim of this study was to determine the prevalence and molecular characterization of trichomonads infecting pet dogs in Anhui and Zhejiang provinces, east China. In total, 315 pet dogs, with or without diarrhea, from 7 pet hospitals were included in this epidemiological survey. Microscopy and PCR detected P. hominis in 19.7% (62/315) and 31.4% (99/315) of fecal samples, respectively. T. foetus infection was detected in 0% (0/315) of samples with microscopy and in 0.6% (2/315) with PCR. The prevalence of P. hominis was significantly higher in young dogs (${\leq}12months$) than in adult dogs (>12 months), and was significantly higher in diarrheic dogs (50.6%) than in non-diarrheic dogs (24.3%; P<0.05). Infection with T. foetus did not correlate with any risk factors evaluated in this study. A sequence analysis of the P. hominis PCR products showed minor allelic variations between our sequences and those of P. hominis strains from other hosts in different parts of the world. Type CC1 was the most common strain in dogs in east China. The internal transcribed spacer 1 (ITS1)-5.8S rRNA gene sequences from the 2 T. foetus isolates detected in this study displayed 100% identity and were homologous to the sequences of other strains isolated from domestic cats in other countries.

Study on the Normal Distribution of Mast Cell in Domestic Animals and It's Response in Various Infections (가축(家畜)의 장기(臟器)와 조직(組織)에 존재(存在)하는 조직비만세포(組織肥滿細胞)의 정상분포(正常分布)와 감염증시(感染症時)의 태도(態度)에 관한 연구(硏究))

  • Kim, Hwa Sik;Lee, Jae Hyun
    • Korean Journal of Veterinary Research
    • /
    • v.12 no.2
    • /
    • pp.165-175
    • /
    • 1972
  • The frequency distribution and morphological characteristics of tissue mast cells in the various tissues of normal domestic animals (bovine, swine, dog and chicken) and systematically infected (Hog cholera, Canine distemper and Newcastle disease) animals were studied. The results were as follows: 1 In cattle, density of mast cells was higher in foetus (bovine) and young animals than in adults Differences in frequency distribution among individual animals were also observed. 2. In chicken, the highest number of mast cells was found in age group of 15 to 40 days, the moderate number in age group of one to 10 days, and the lowest number in age group of 40 days or older. 3. The morphologically, mast cells were usually round, ovoid, spindle, oval and irregular, and particularly in ovary of bovine, it was usually round and ovoid forms. 4. The largest numbers of mast cells were shown in ovary of bovine, intestine of swine and dog, and proventriculum of chicken. 5. In the systemic infections, the number of mart cells usually tends to increase.

  • PDF

Parasitological and Biochemical Approaches of Studies on Korean Cattle Showing Reproductive Disorders (한우(韓牛)의 증식저해(增殖沮害) 요인(要因)에 관한 연구(硏究) -기생충학적(寄生蟲學的) 및 의화학적(醫化學的) 조사(調査)-)

  • Jang, Du Hwan;Shin, Jae Doo
    • Korean Journal of Veterinary Research
    • /
    • v.15 no.2
    • /
    • pp.327-332
    • /
    • 1975
  • Parasitological and biochemical studies for 82 heads (12.2%) showing sterility syndrom out of 672 heads of Korean native cattle were undertaken by analysis of blood values for their contents and examination of their vaginal swabs for Tritrichomonas foetus 1. Tritrichomonas infection of Korean cattle revealed 7 positive cases (8.54%) out of 82 infertile heads. 2. Contents of their serum protean, carotenoid, vitamin A and phosphorus were determined in summer and winter season and the following results were obtained. a) In summer season, average blood value of 30 cattle showing sterility syndrom were determined as protein 7.52g/100ml, carotenoid 165.1g/100ml, vitamin A 173.9 IU/100ml and phosphorus 7.48mg/100ml, respectively. b) In winter. season, average blood value of 52 cattle showing sterility syndrom were determined as protein 7.76g/100ml, carotenoid 413.3g/100ml, vitamin A 174,3 IU/100ml and phosphorus 7.54mg/100ml, respectively.

  • PDF

Studies on the Radiosensitivity of the Chromosomes in Cultured Human Cells (사람의 배양세포염색체의, 방사선감수성에 관한 연구)

  • 강영선;김영진;이정길
    • The Korean Journal of Zoology
    • /
    • v.10 no.1
    • /
    • pp.17-20
    • /
    • 1967
  • The present experiment was perform to investigate the frequencies of chromosome aberration with special regard to the chromosome groups and the various time intervals after X-irradiation (60 r) in ormal human foetus cells grown in culture. The cytological preparations were prepared at every 5 through 30 hours after X-irradiation by the air-drying method. 1. The frequencies of chormosome aberration are on the whole decreased as tie elapses after irradiation and this is thought to be due to gradual recovery with time . However, a slight increase in frequencies is observed at 25 and 30 hours after irradiation respectively. This shows that the cells at the these periods are more sensitivity to X-irradation , and those cells are thought to be at G$_2$ and late S stage at the time of irradiation respectively, so t is evident that G$_2$ and late S stages a the time of irradiation respectively , so it is evident that G$_2$ and late S stages are more sensitive to X-irradiation than any other stages. 2. The frequencies of chormosome aberration are decreased in descending order of chormosome group number. The differences among these frequencies are highly significant statistically . Therefore it can be concluded that there is a highly significant difference in radiosensitivity among chromosome groups. that is, the chromosomes of the group A are the most radiosensitive , followed by B, C, D ,E and G in descending order.

  • PDF