• Title, Summary, Keyword: GC/MS

Search Result 2,263, Processing Time 0.033 seconds

Structural Determination of Cerebrosides from Soybean Embryo by Mass Spectrometer (Mass Spectrometer를 이용한 대두 배아 출추 Cerebroside의 구조 분석)

  • Kim, Jung-Hun;Chang, Sug-Youn;Kim, Yeo-Kyung
    • Analytical Science and Technology
    • /
    • v.6 no.3
    • /
    • pp.335-343
    • /
    • 1993
  • The structure of cerebrosides from soybean embryo was determined using fast atom bombardment mass spectrometer (FAB-MS), gas chromatography mass spectrometer (GC-MS) and TLC. The components of cerebroside were determined by GC-MS after acid hydrolysis. The molecular weight distribution of cerebroside was measured by positive mode FAB-MS with LiOH saturated 3-nitrobenzylalcohol(3-NBA) matrix. Structures of individual components of complex mixtures can be determined easily by this process. The major constituent of soybean extracted cerebroside was determined as the glucoside of N-2'-hydroxypalmitoyl-sphingadienine.

  • PDF

Application of Comprehensive 2D GC-MS and APPI FT-ICR MS for More Complete Understanding of Chemicals in Diesel Fuel

  • Cho, Yun-Ju;Islam, Annana;Ahmed, Arif;Kim, Sung-Hwan
    • Mass Spectrometry Letters
    • /
    • v.3 no.2
    • /
    • pp.43-46
    • /
    • 2012
  • In this study, comprehensive two dimension gas chromatography (2D GC-MS) and 15 T Fourier transform ion cyclotron resonance mass spectrometry (15T FT-ICR MS) connected to atmospheric pressure photo ionization (APPI) have been combined to obtain detailed chemical composition of a diesel oil sample. With 2D GC-MS, compounds with aliphatic alkyl, saturated cyclic ring(s), and one aromatic ring structures were mainly identified. Sensitivity toward aromatic compounds with more than two aromatic rings was low with 2D GC-MS. In contrast, aromatic compounds containing up to four benzene rings were identified by APPI FT-ICR MS. Relatively smaller abundance of cyclic ring compounds were found but no aliphatic alkyl compounds were observed by APPI FT-ICR MS. The data presented in this study clearly shows that 2D GC-MS and 15T FT-ICR MS provides different aspect of an oil sample and hence they have to be considered as complementary techniques to each other for more complete understanding of oil samples.

Constructing Database for Drugs and its Application to Biological Sample by HPTLC and GC/MS (HPTLC와 GC/MS를 이용한 의약품의 데이타베이스화 및 생체시료에의 응용)

  • Yoo, Young-Chan;Park, Sung-Woo;Lim, Mie-Ae;Baeck, Seung-Kyung;Park, Seh-Youn;Lee, Ju-Seon;Lho, Dong-Seok
    • Analytical Science and Technology
    • /
    • v.13 no.2
    • /
    • pp.136-150
    • /
    • 2000
  • For the identification of unknown drugs in biological samples, we attempted rapid high performance thin layer chromatographic method which is sensitive and selective chromatographic analysis of high performance thin layer chromatography (HPTLC) with automated TLC sampler and ultra-violet (UV) scanner. We constructed HPTLC database (DB) on two hundred five drugs by using the data of Rf values and UV spectra (scan 200-360 nm) as well as gas chromatography/mass spectrometry (GC/MS) DB on ninety six drugs by using the data of relative retention time (RRT) on lidocain and mass spectra. After extracting drugs in biological sample by solid phase extraction (Clean Screen ZSDAU020), we applied them to HPTLC and GC/MS DB. Drugs, especially extracted from biological samples, showed good matching ratio to HPTLC DB and these drugs were confirmed by GC/MS. In conclusion, this DB system is thought to be very useful method for the screening of unknown drugs in biological samples.

  • PDF

GC/MS and GC/ECD Analysis of Residual Pesticides in Herbal drugs (GC/MS와 GC/ECD를 이용한 한약재 중 잔류 농약의 분석)

  • Kim, Ho-Kyoung;Park, So-Yeon;Ko, Byoung-Seob
    • The Journal of Korean Medicine
    • /
    • v.20 no.1
    • /
    • pp.44-51
    • /
    • 1999
  • Analysis of residual pesticides in herbal drugs was performed by GC-ECD and GC-MS. Especially, selected ion monitoring(SIM) technique was applied to increase the GC/MS sensitivity. Analysis of residual pesticides was determined sensitivity and selectively without any internal standard by setting the SIM technique to their characteristic fragments for quantitation ion and confirmation ion. The combination of two detector, GC-ECD and MS-SIM technique, is abailable for determining a multiclass residual pesticides in herbal drugs. The average recoveries through the method were $65.9%{\sim}99.7%$ in herbal drugs. The data of gas chromatographic analysis was compared with the limits of residual pesticides in herbal drugs and agricultural foods. 4. 4-DDT was detected above the limits to the residual pesticides in herbal drugs. Diazinon and EPN were detected, but the limits of residual pesticides were less than that of agricultural foods.

  • PDF

Quantitative Analysis of the Seven Marker Components in Asarum sieboldii using the LC-MS/MS and GC-MS (LC-MS/MS와 GC-MS를 이용한 세신 추출물 중 7종 성분의 함량분석)

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • Korean Journal of Pharmacognosy
    • /
    • v.44 no.4
    • /
    • pp.350-361
    • /
    • 2013
  • Asarum sieboldii has been used for treatment of fever, pain, common cold, and chronic sinusitis in Korea. In this study, we performed quantification analysis of seven major constituents including aristolochic acid I, aristolochic acid II, ${\alpha}$-asarone, ${\beta}$-asarone, elemicin, methyl eugenol, and safrole in the 70% ethanol extract of Asarum sieboldii and its solvent fractions, n-hexane, ethylacetate, n-butanol, and water ones using a ultra-performance liquid chromatography-electrospray ionization-mass spectrometer(UPLC-ESI-MS) and gas chromatography-mass spectrometer(GC-MS). Regression equations of seven components were acquired with $r^2$ values >0.99. The values of limit of detection(LOD) and quantification(LOQ) were 0.1-3.9 ng/mL and 0.3-11.7 mg/mL, respectively. The amount of the seven compounds in Asarum sieboldii were not detected -143.66 mg/g. The established LC-MS/MS and GC-MS methods will be helpful to improve quality control of Asarum sieboldii.

Multi-residue Analysis of Pesticides using GC-TOF/MS, ECD, NPD with QuEChERS Sample Preparation (QuEChERS 전처리법과 GC-TOF/MS, ECD, NPD를 이용한 잔류농약 다성분분석)

  • Park, Jungwook;Kim, Aekyung;Kim, Jongpil;Lee, Hyanghee;Park, Duckwoong;Moon, Sujin;Ha, Dongryong;Kim, Eunsun;Seo, Kyewon
    • The Korean Journal of Pesticide Science
    • /
    • v.18 no.4
    • /
    • pp.278-295
    • /
    • 2014
  • Fast and accurate multi-residue pesticides inspecting method needs in Agro-Fishery Products Inspection Center. So, We tried to seek the optimum method using GC-TOF/MS, GC-ECD, GC-NPD after QuEChERS sample preparation. In GC-TOF/MS, 138 kinds of pesticide were spiked at 0.3 and $0.5{\mu}g/g$for the identification and quantification in lettuce sample. Recoveries of 77 pesticides were between 70 and 130% with RSD (relative standard deviation lower than 20% at $0.3{\mu}g/g$. In GC-ECD, NPD, 146 kinds of pesticide were spiked for the identification and quantification in lettuce. Recoveries of 61 species were between 70 and 130% with lower than 20%. These results indicated that GC-TOF/MS, GC-ECD, NPD analysis with the QuEChERS sample preparation can be partly applied to multi-residue pesticides in vegetables.

Analysis of Residual Pesticides in Herbal Drugs: GC/MS Analysis of 27 Controlled Pesticides (생약 중 잔류 농약의 분석법: GC/MS에 의한 27종 잔류 규제 농약의 분석)

  • Park, Man-Ki;Park, Jeong-Hill;Yoon, Hye-Ran;Yoon, In-Byoung;Cho, Sool-Yeon;Hwang, Gwi-Seo
    • YAKHAK HOEJI
    • /
    • v.40 no.2
    • /
    • pp.141-148
    • /
    • 1996
  • GC/MS analysis of 27 controlled pesticides in herbal drugs was studied. Selected ion monitoring(sim) technique was applied to increase the GC/MS sensitivity. Typical peaks in th e mass spectrum of each pesticides were selected as quantitation, comfirmation or alternate ion. Twenty seven pesticides were divided into five groups according to their retention time and the peaks for SIM were programmed accordingly. The combination of two ionization methods, electron impact(EI)-SIM-MS and negative ion chemical ionization(NCI)-SIM-MS, were well-fitted for the detection, confirmation and quantitation of multiclass residual pesticides in herbal drugs.

  • PDF

Analysis of Cyclofenil and its Metabolites in Human Urine using GC/MS (GC/MS를 이용한 소변중의 Cyclofenil 및 그 대사체의 분석)

  • Myung, Seung-Woon;Min, Hye-Ki;Cho, Hyun-Woo;Kim, Dong-Hyun;Kim, Myungsoo
    • Analytical Science and Technology
    • /
    • v.15 no.3
    • /
    • pp.185-189
    • /
    • 2002
  • International Olympic Committee (IOC) prohibits the use of cyclofenil for male. To prove whether cyclofenil product was taken or not, the analytical method in urine using GC/MS was established. The extraction recoveries of cyclofenil and its metabolites were higher in the range of pH 5-9. Because the parent drug (cyclofenil) was not excreted in urine, metabolites were detected and theses were used as a criteria of cyclofenil dose. Therefore the metabolites were hydrolyzed, extracted at pH 9.6, derivatized with MSTFA and analyzed by GC/MS.

GC-MS Analysis of Chemical Constituents from Various Agarwood (GC-MS를 이용한 침향류의 성분 비교 연구)

  • Shin, Kwhang-Ho;Choi, Kyu-Yeol;Cho, Sung-Yong;Ahn, Duk-Kyun;Park, Seong-Kyu
    • The Korea Journal of Herbology
    • /
    • v.26 no.1
    • /
    • pp.7-12
    • /
    • 2011
  • Objectives : The purpose of this study is to evaluate the quality of four different kinds of agarwood and analysis chemical constituents by GC-MS. Methods : Four source plants were two of Vietnamese agarwood, Indonesian agarwood and Myanmar agarwood. These plant materials were named sample No. 1-4. The chemical constituents of each agarwood were analysed by GC-MS. Results : GC-MS analysis showed that ${\beta}$-selinene was found in all the samples. The Vietnamese agarwood, Sample 1, and 4 has contained 6.861% and 7.497% of ${\beta}$-selinene and reached the highest level at the retention times 46.245 and 46.274 (min). Sample 2, the Myanmar agarwood has contained 1.608%, the lowest level of ${\beta}$-selinene, and it also has contained about 20% of ${\alpha}-,{\beta}-,{\gamma}$-eudesmol and 4.076% of valerianol. Sample 3, the Indonesian agarwood has contained 9.59% of ${\beta}$-selinene, 9.493% of ${\beta}$-selinene and shown its highest level (more than 19%) at the retention time 43.998 (min). Conclusion : It suggested that ${\beta}$-selinene could be a standard marker to evaluate agarwood by GC-MS analysis.