• Title, Summary, Keyword: Gallic acid

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Characterization of Lactobacilli with Tannase Activity Isolated from Kimchi

  • Kwon, Tae-Yeon;Shim, Sang-Min;Lee, Jong-Hoon
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1322-1326
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    • 2008
  • Tannase catalyzes the hydrolysis of gallic acid esters and hydrolysable tannins. Twenty-two Lactobacillus strains with tannase activity were isolated from 7 types of kimchi. A polymerase chain reaction-based assay targeting the recA gene assigned all isolates to either Lactobacillus plantarum or Lactobacillus pentosus. The tannase activities of isolates measured in whole cells and cell-free extracts varied even within each species. The activities of the isolates varied with the assay method, but both methods indicated that isolate LT7 (identified as L. pentosus) showed the highest activity. The results of thin layer chromatography and high performance liquid chromatography, respectively, showed that tannic acid and gallic acid degraded to pyrogallol in resting L. pentosus LT7 cells. Therefore, the putative biochemical pathway for the degradation of tannic acid by L. pentosus implies that tannic acid is hydrolyzed to gallic acid and glucose, with the formed gallic acid being decarboxylated to pyrogallol. This study revealed the possible production of pyrogallol from tannic acid by the resting cell reaction with L. pentosus LT7.

Industrial scale extraction and stripping devices for continuous recovery of gallic acid from Chinese nutgall processing wastewater

  • Wu, Yundong;Xia, Xihe;Dong, Shuyu;Zhou, Kanggen
    • Environmental Engineering Research
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    • v.22 no.3
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    • pp.288-293
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    • 2017
  • In this study, we report the industrial application of an efficient technology for the recovery of gallic acid from Chinese nutgall processing wastewater. The recovery of gallic acid by industrial scale extraction and stripping devices was performed, with tributyl phosphate as the extractant and kerosene as the diluent. The results showed that the theoretical extraction stage was four, while the theoretical stripping stage was two. A closed-cycle system was studied for the continuous countercurrent extraction and stripping, with a five-stage extraction device and a three-stage reflux stripping device. The results showed that the multistage extraction-stripping system could steadily run for a long period, the average gallic acid level in the raffinate was $0.85g{\cdot}L^{-1}$, and the gallic acid content recovered in the strip liquor was higher than $120g{\cdot}L^{-1}$. The average extraction yield of gallic acid was 94.14%. When the strip liquor was used as raw material for production, the average production yield increased by 8.64%. In addition, after extraction, the $COD_{Cr}$ in the wastewater decreased by 38.19%, and the biodegradability of wastewater improved by 1.6 times. This study provided a new impetus for the sustainable development of the Chinese nutgall processing industry.

Antiviral Activity of Gallic Acid against Coxsackievirus B3 and Coxsackievirus B4 (Gallic acid의 Coxsackievirus B3와 Coxsackievirus B4에 대한 항바이러스 효과)

  • Choi, Hwa-Jung
    • Microbiology and Biotechnology Letters
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    • v.42 no.2
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    • pp.207-210
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    • 2014
  • Viral infections are capable of inducing reactive oxygen species (ROS) production in the infected cells and antioxidants have been reported to have antiviral activities against many viruses. In this study, an antiviral assay using the cytopathic effect (CPE) reduction method revealed that gallic acid possesses good anti-coxsackievirus B3 (CB3) and coxsackievirus B4 (CB4) activities, reducing the formation of visible CPE. However, ribavirin did exhibit weak anti-CB3 and CB4 activities and was unable to prevent CPE. Therefore, we conclude that the inhibition of CB3 or CB4 production by gallic acid may be due to its general action as an antioxidant.

A Study on the Tannin Components and Physical Properties of Acorn Starch - Gallic Acid Contents and Viscosity - (도토리 전분(澱粉)의 Tannin 성분(成分)과 물리적(物理的) 특성(特性)에 관(關)한 연구(硏究) - Gallic Acid 함양(含量)과 점도특성(粘度特性) -)

  • Park, Jae-Young;Koo, Sung-Ja
    • Journal of Nutrition and Health
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    • v.17 no.1
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    • pp.41-49
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    • 1984
  • Tannin from acorn was identified by TLC and gallic acid in the tannin was determined by HPLC. The tannin extracted with methanol-butanol was not dissolved in ethanol, methanol for HPLC and water, while the tannin extracted with acetone-ethylacetate was so pure that it could be used for HPLC-running. The gallic acid showed a Rf -value of 0.39, the digallic acid of 0.21, the trigallic acid of 0.09, and the gallotannin of 0.00 respectively. The content of gallic acid in the tannin from acorn powder was 3.04%, from acorn starch, 0.90%, and from acorn starch gels, 0.64-0.86% respectively. The effect of tannin contents on the viscosity of acorn atarch was also studied. The maximum and cooling viscosity of the starch were decreased as the contents of tannin increased.

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Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Gallic acid 등으로 유발된 인간 간 조직세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향)

  • Park, Wan-Su;Kim, Do-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.6
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    • pp.1379-1384
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    • 2009
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFL (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFL could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.

Anti-thrombotic Effects of Analogs of Protocatechuic Acid and Gallic Acid (Protocatechuic acid 및 Gallic acid 유도체들의 항 혈전작용)

  • 윤혜숙;강삼식;김문희;정기화
    • YAKHAK HOEJI
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    • v.37 no.5
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    • pp.453-457
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    • 1993
  • Platelet anti-aggregating activities were tested with analogs of protocatechuic acid and gallic acid. Six of them which showed comparable inhibitory effects with aspirin against collagen induced platelet aggregation were selected and their anti-thrombotic effects were evaluated in the mouse thrombosis model and compared with those of aspirin and paeonol. At the dose of 50 mg/kg, p.o., ethyl gailate(13) treated group showed higher % of recovery within 6 min of thrombotic challenge and lower mortality within 5 min than aspirin treated group.

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Phenolic Acid Composition and Antioxidative Activity of Chestnut Endoderm (밤 삽피의 페놀산 조성과 항산화 활성)

  • Kim, Young-Chan;Kim, Mi-Yeon;Chung, Shin-Kyo
    • Applied Biological Chemistry
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    • v.45 no.3
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    • pp.162-167
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    • 2002
  • Free, soluble esterified and insoluble bounded phenolic acids were separated from Eungi chestnut endoderm. The composition and contents of phenolic acid were analyzed by gas chromatography, and their antioxidant activity was examined by DPPH assay, 2-deoxyribose oxidation, and ferric thiocyanate method. Gallic, ellagic, salicylic, and gentisic acids in free phenolic acid fraction, gallic, ellagic, and protocatechuic acids in soluble esterified fraction, sianpic and gentisic acids were the major phenolic acids in insoluble bounded fraction. Marked differences were observed in the phenolic acid composition and contents among the fractions. Free phenolic acid fraction showed the strongest antioxidant activity. Results revealed chestnut endoderm could be a potential antioxidant source containing gallic and ellagic acids.

Utilization of Biomass Resources(II) Analysis of Polyphenol Components and Antioxidative Activities from Chestnut Inner Bark (Biomass 자원의 활용(II) 율추로부터 폴리페놀 성분의 분석과 항산화활성)

  • Jo, Jong-Soo;Kim, Yun-geun
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.6
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    • pp.71-78
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    • 2005
  • For the utilization of chestnut inner bark as forest biomass, this studies have been carried out cleary investigated about phenolic compounds from the diethyl ether solubles and ethyl acetate solubles of hot water extract from chestnut inner bark. 3 compound were isolated and their structures were identified by spectroscopic methods. They were known compounds, 3,5-dihydroxybenzoic acid, 3,4,5-trihydrixybenzoic acid and catechin. Electron donating ability (EDA) of 3 compounds were investigated. Gallic acid and catechin were found to higher EDA than control, ascorbic acid and $\alpha$-tocopherol in 10 ppm, and especially gallic acid has very higher EDA, 21 times than ascorbic acid. This gallic acid of chemical structure were substituted with free phenolic hydroxyl groups at meta and para position. It was supposed that phenolic hydroxyl groups in chemical structure was a EDA factor.

Induction of Extracellular Polyphenol Oxidase from Two White-rot Fungi (목재(木材) 부후균(腐朽菌)의 목질소(木質素) 분해효소(分解酵素) 유도(誘導)에 관하여)

  • Kim, Kyu-Jung;Shin, Kwang-Soo;Hong, Soon-Woo
    • The Korean Journal of Mycology
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    • v.14 no.1
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    • pp.43-47
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    • 1986
  • Among the representative phenolic compounds in relation to lignin derivatives and protein synthesis inhibitors, the most effective inducer for the extracellular polyphenol oxidase (PO) of Lentinus edodes JA01 was gallic acid and ferulic acid for Pleurotus ostreatus. Optimum concentration of these inducers was 2.0 mM and 1.0 mM, respectively. Addition of gallic acid after two days culture had the best effect on production of PO enzyme of L. edodes JA01 and for P. ostreatus, and addition of ferulic acid after three days culture had the best effect. Also, in case of L. edodes JA01, polyphenol oxidase activity was parallel to growth curve, whereas the maximum enzyme activity of P. ostreatus was shown at exponential growth phase and declined thereafter.

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Effects of Phenolic Compounds in Milled Barley Grains on the Growth of Saccharomyces cerevisiae (보리쌀중의 Phenol 화합물이 Saccharomyces cerevisiae의 생육에 미치는 영향)

  • 정기택;김욱한;송형익
    • Korean Journal of Microbiology
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    • v.24 no.2
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    • pp.168-174
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    • 1986
  • The phenolic compounds contained in milled barley grains were seperated and identified by gas liquid chromatography and the effects of phenolic compounds extracted from milled barley grains and each authentic phenolic compound on the growth of Saccharomyces cerevisiae were studied. Severn phenolic acids, namely cinnamic, protocatechuic, ferulic, sinapid, vanillic, syringic, gallic acids, were identified in milled barley grains by gas liquid chromatography. The contents of sinapic, ferulic, cinnamic, protocatechuic acids were larger than those of vanillic and gallic acids. Phenolic compounds, extracted from milled barley grains and supplemented in culture broth, were inhibitory to the growth of Saccharomyces cerevisiae at levels above 100ppm to 24 hours but not inhibitory at all levels after 48 hours. Cinnamic, ferulic, vanillic acids at all levels were inhibitory to the growth of Saccharomyces cerevisiae, among them cinnamic acid was most inhibitory. Syringic acid was inhibitory to the growth of the yeast at the initial stage of culture. But sinapic and protocatechuic acids were slightly stimulatory to the growth of the yeast and gallic acid was ineffective to the growth of the yeast.

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