• Title, Summary, Keyword: Globotriosyl ceramide

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Quantitative assay of glycosphingolipid and determination of binding affinity for Shigatoxin 2e in pig (돼지의 일령에 따른 glycosphingolipid의 정량 및 Shigatoxin 2e에 대한 친화성 확인)

  • Dong, Bun-youn;Kim, Sang-Hyun;Cho, Hyun-Ho;Kim, Yeong-Il;Lee, Woo-won;Kim, Kon-Sup;Kang, Ho-Jo;Kim, Yong-Hwan
    • Korean Journal of Veterinary Research
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    • v.44 no.2
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    • pp.233-239
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    • 2004
  • This study was designed to quantitative assay of cellular receptor globotriosyl ceramide($Gb_3$) and globotetraosyl ceramide($Gb_4$) and to determine binding affinity of the receptors for Shigatoxin 2e in difference with day olds of pig. The amount of $Gb_3$ and $Gb_4$ extracted from each swine tissue were detected in order of spleen, ileocecum, kidney, and jejunum. The amount of $Gb_3$ and $Gb_4$ extracted from each swine ileocecum and jejunum in different day old swine were detected in order of 24, 6, 98, 12 and 35, but the difference of amount was not significant. The binding affinity of $Gb_4$ extracted from ileocecum and jejunum with different day old swine for Stx2e were shown that the extracts of 24, 12, and 35 days old swine were higher significantly than that of 6 and 98 days old swine.