• Title, Summary, Keyword: Gluconacetobacter hansenii

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Production of Bacterial Cellulose by Gluconacetobacter hansenii PJK Isolated from Rotten Apple

  • Park, Joong-Kon;Park, Youn-Hee;Jung, Jae-Yong
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.2
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    • pp.83-88
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    • 2003
  • A cellulose-producing strain isolated from rotten apples was identified as Gluconacetobacter hansenii based on its physiological properties and the 16S rDNA complete sequencing method, and specifically named Gluconacetobacter hansenii PJK. The amount of bacterial cellulose (BC) produced by G. hansenii PJK in a shaking incubator was 1.5 times higher than that produced in a static culture. The addition of ethanol to the medium during cultivation enhanced the productivity of bacterial cellulose, plus the supplementation of 1% ethanol into the culture medium made the produced BC aggregate into a big lump and thus protected the bacterial-cellulose-producing G. hansenii PJK cells in the shear stress field from being converted into non-cellulose-producing (Cel) mutants. Cells subcultured three times in a medium containing ethanol retained their ability to produce BC without any loss in the production yield.

Effect of Medium Composition on the Bacterial Cellulose Production by Gluconacetobacter hansenii PJK (배지조성이 Gluconacetobacter hansenii PJK의 Bacterial Cellulose 생산에 미치는 영향)

  • 정재용;박연희;박중곤
    • KSBB Journal
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    • v.18 no.2
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    • pp.94-99
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    • 2003
  • The effect of medium composition on the production of bacterial cellulose (BC) by Gluconacetobacter hansenii PJK was investigated. The addition of yeast extract and peptone in the medium increased the production yield (Y/sub p/s/) of BC. The amount of BC produced by G. hansenii PJK was constant if the initial pH of the medium was in the range 4.5 to 6.0. Strains from the supernatant of the culture medium produced more BC than those from inside the BC. BC production was dependent on glucose metabolism, and the addition of fructose or lactate as a carbon source converted cells to Cel/sup -/ mutants. Cel/sup -/ mutants produced by the addition of fructose or lactate to the medium caused 73% or 30% decreases in BC production, respectively. The addition of succinate, which is one of the constituents of the TCA cycle, did not affect the production of BC.

Pilot Production of Bacterial Cellulose by Gluconacetobacter hansenii TL-2C (Gluconacetobacter hansenii TL-2C에 의한 Bacterial Cellulose의 Pilot 생산)

  • Jeong, Ji-Suk;Kim, Jong-Sun;Choi, Kyoung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.10
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    • pp.1341-1350
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    • 2007
  • This study was designed to ultimately develop a highly efficient mass production technology of bacterial cellulose isolated from the citrus gel fermented by G. hansenii TL-2C. Pilot equipment made with FRP vessel length (665 mm) ${\times}$ width (375 mm) ${\times}$ height (210 mm) was developed for mass production of the citrus gel. To develop the optimal conditions for mass production of citrus gel, comprised of citrus juice (6,000 mL) diluted 100 times, containing 5% seed bacteria, 10% sucrose, and 1% ethanol, citrus juice was fermented at $30^{\circ}C$ for 14 days, and gel productivity in pilot system was examined. BC was isolated and purified from the citrus gel, and their chemical composition and physicochemical properties were investigated.

Optimization of Culture Condition of Gluconacetobacter hansenii TF-2 for Cellulose Gel Production (Gluconacetobacter hansenii TF-2를 이용한 감귤과즙으로부터의 셀루로스 겔 생산의 최적화)

  • 최경호;정지숙;문철호;김미림
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.1
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    • pp.176-181
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    • 2004
  • Gluconacetobacter hansenii TF-2, an isolate from black tea fungus, was statically cultivated to ferment cellulose gel from citrus juice. The juice prepared by press filtering of peeled citrus fruit contained 135.5 mg of total sugar/mL, 1.23% of total acid, and average pH of the juice was 3.98. The bacterium produced cellulose gel optimally on the surface of culture broth containing 17% of citrus juice and 10$^{\circ}$Brix of total sugar. The optimum temperature was 3$0^{\circ}C$ for producing acetic acid and gel formation. The bacterium could not produce acetic acid on gel formation at 4$0^{\circ}C$. The optimum pH was 3.0∼4.0 but was not significantly different between pH 3.0∼4.0. The cultivation for 18 days under optimal conditions produced gel as 14.2$\pm$0.6 mm of thickness and acids equivalent to 1.90$\pm$0.22% of acetic acid. The pH of culture broth was stabilized at 2.6∼2.8 during the cultivation. Remaining sugar content was 27.1$\pm$4.2 mg/mL of total sugar and 6.9 mg/mL of reducing sugar. The gel productivity was 137.8$\pm$9.7 g/L.

Statistical Optimization of Medium Composition for Bacterial Cellulose Production by Gluconacetobacter hansenii UAC09 Using Coffee Cherry Husk Extract - an Agro-Industry Waste

  • Rani, Mahadevaswamy Usha;Rastogi, Navin K.;Anu Appaiah, K.A.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.7
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    • pp.739-745
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    • 2011
  • During the production of grape wine, the formation of thick leathery pellicle/bacterial cellulose (BC) at the airliquid interface was due to the bacterium, which was isolated and identified as Gluconacetobacter hansenii UAC09. Cultural conditions for bacterial cellulose production from G. hansenii UAC09 were optimized by central composite rotatable experimental design. To economize the BC production, coffee cherry husk (CCH) extract and corn steep liquor (CSL) were used as less expensive sources of carbon and nitrogen, respectively. CCH and CSL are byproducts from the coffee processing and starch processing industry, respectively. The interactions between pH (4.5-8.5), CSL (2-10%), alcohol (0.5-2%), acetic acid (0.5-2%), and water dilution rate to CCH ratio (1:1 to 1:5) were studied using response surface methodology. The optimum conditions for maximum BC production were pH (6.64), CSL (10%), alcohol (0.5%), acetic acid (1.13%), and water to CCH ratio (1:1). After 2 weeks of fermentation, the amount of BC produced was 6.24 g/l. This yield was comparable to the predicted value of 6.09 g/l. This is the first report on the optimization of the fermentation medium by RSM using CCH extract as the carbon source for BC production by G. hansenii UAC09.

Production of Bacterial Cellulose Using Waste of Beer Fermentation Broth (맥주발효 폐액을 이용한 미생물 셀룰로오스 생산)

  • Park, Joog Kon;Hyun, Seung Hoon;Ahn, Won Sool
    • Korean Chemical Engineering Research
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    • v.44 no.1
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    • pp.52-57
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    • 2006
  • Bacterial cellulose (BC) was produced by Gluconacetobacter hansenii PJK (KCTC 10505 BP) strains using the waste of beer fermentation broth. It contained more C and N than a basal medium with a small amount of S and more than 4% ethanol. The amount of BC produced in a shaking culture using the waste of beer fermentation broth was nearly the same as that of a basal medium. The production of BC decreased in a shear stress field in a jar fermenter although the conversion of cellulose producing ($Cel^+$) cells to non-cellulose producing ($Cel^-$) mutants was not severe. This study showed that the waste of beer fermentation broth is an inexpensive carbon, nitrogen source with ethanol and thus a worthy substitute for the conventional medium for BC production.

Production of Bacterial Cellulose by Gluconacetobacter hansenii Using a New Bioreactor Equipped with Centrifugal Impellers (원심 임펠러가 장착된 발효조에서 G. hansenii에 의한 미생물셀룰로오스 생산)

  • Khan, Salman;Shehzad, Omer;Khan, Taous;Ha, Jung Hwan;Park, Joong Kon
    • Korean Chemical Engineering Research
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    • v.47 no.4
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    • pp.506-511
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    • 2009
  • In order to improve the bacterial cellulose(BC) production yield, centrifugal and inclined centrifugal impellers were developed. A 6 flat-blade turbine impeller was used as a control system. The flow pattern in the fermenter and volumetric oxygen transfer coefficient($k_La$) of these fermentation systems were studied. Fermentations were carried out for the production of BC by G. hansenii PJK in a 2-L jar fermenter equipped with new impellers. Liquid medium was circulated from the bottom, through the cylinder of the impeller and to the wall. The volumetric oxygen transfer coefficients, $k_La$, of inclined centrifugal and centrifugal impeller systems at 100 rpm were 23 and 15% of the conventional turbine impeller system, respectively. However, the conversion of microbial cells to cellulose non-producing mutant decreased and this results in the increase in BC production at low rotating speed of impellers.