• Title, Summary, Keyword: Goat

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Regulatory Sequences in the 5' Flanking Region of Goat β-Casein Gene

  • Huang, Mu-Chiou;Chao, Jiunn-Shiuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.11
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    • pp.1628-1633
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    • 2001
  • A goat ${\beta}$-casein gene was cloned and sequenced. Our previous study had determined the nucleotide sequences of the 5' flanking region and the structural gene including all 9 exons. In the present study, investigations were done on the regulatory sequences in the 5' flanking region of the goat ${\beta}$-casein gene by aligning and comparing it with the same gene from other mammals. The results showed that -200/-1 bp of the 5' flanking sequences contained six conserved clusters, in which the sites of gene expression regulated by the transcription factor and hormone might exist. It showed that fourteen glucocorticoid receptor elements, two cAMP responsive elements, two SV40 virus enhancer core sequences, two OCT-1 binding elements and one CTF/NF-1 binding element were dispersed in the 5' flanking region of goat ${\beta}$-casein gene. Our findings are perhaps valuable for the elucidation of the molecular mechanisms that control the expression of the goat ${\beta}$-casein gene.

Indirect Assessment of Sperm Capacitation Using Zona-free Hamster Eggs in the Goat I. Penetration into Zona-free Hamster Eggs by Goat Spermatozoa Preincubated in the Uteri Isolated from Hamsters and Rats

  • Song, H.B.;Iritani, A.
    • Korean Journal of Animal Reproduction
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    • v.9 no.2
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    • pp.148-152
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    • 1985
  • When goat spermatozoa were preincubated for 4-6 h and 6 h in the uteri isolated from hamster and rat, and for 6 h in the hamster uterus in situ, they developed the ability to penetrate zona-free hamster eggs in vitro. Zona-free hamster eggs were not penetrated after insemination with goat spermatozoa preincubated in the isolated hamster uterus 4 h before and 2 h after expected time of ovulation, respectively. Zona-free hamster eggs were not penetrated after insemination with goat spermatozoa preincubated for 4 h in the isolated hamster uterus, but 10 and 18% of eggs were penetrated by spermatozoa preincubated for 5 and 6 h in the isolated uterus.

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Erythrocyte Sedimentation Rates of Reshuffled Packed Cell Volume in Korean Native Goat (PVC수치와 온도가 한국재래 산양적혈구 침강에 미치는 영향)

  • Lee, Soo Doo;Kim, Young Hong;Yu, Chang Jun
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.55-59
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    • 1993
  • The packed cell volume(PCV) of Korean native goat, volume percentage of red blood cell in whole blood, was reshuffled of 20%, 40% and 60% using autoplasma, and erythrocyte sedimentation rate was measured in Westergren tubes at room temperature ($27{\pm}1^{\circ}C$) and low temperature ($8{\pm}1^{\circ}C$). The sedimentation rates of red blood cell obtained are summarized as follows. The erythrocyte sedimentation rates of Korean native goat are accelerated more at high temperature than low temperature. The erythrocyte sedimentation rates of reshuffled Korean native goat upon time are almost linear for several hours. The erythrocyte sedimentation rates of Korean native goat are settled faster at low PCV than higher PCV, i. e., there is a reverse relationshif between the erythrocyte sedimentation rate and packed cell volume.

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An immunohistochemical study of the endocrine cells in the common pancreatic ducts of the Korean native goat (한국재래산양의 대췌관에서 내분비세포의 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.37 no.2
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    • pp.263-267
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    • 1997
  • The distribution and relative frequencies of the endocrine cells were studied immunohistochemically in the common pancreatic ducts of the Korean native goat with serotonin, glucagon, insulin, BCG, BPP and somatostatin antisera. Serotonin-, glucagon-, BCG-, BPP- and somatostatin-immunoreactive cells were detected in the basal portion of the mucosal gland in the common pancreatic ducts of the Korean native goat but insulin-immunoreactive cells were not detected. The function of these immunoreactive cells and appearance of other immunoreactive cells in the common pancreatic ducts of the Korean native goat were remains unknown.

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Occurrence of Goat's-Beard Powdery Mildew Caused by Podosphaera ferruginea in Korea

  • Lee, Sang-Yeob;Kim, Wan-Gyu;Hong, Sung-Kee;Lee, Young-Kee
    • The Plant Pathology Journal
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    • v.25 no.3
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    • pp.299-301
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    • 2009
  • Powdery mildew symptoms were frequently on plants of goat's-beard (Aruncus dioicus var. kamtschaticus) found cultivated in the island Ulleungdo in June 2008. The symptoms appeared as powdery mycelial colonies and dark brown cleistothecia on leaves, petioles, stems and budding flowers of the plant. Incidence of the disease was as high as 50-80% in the goat's-beard fields. Specimens of the diseased plants were collected and examined for morphological characteristics of the causal fungus. On the basis of morphological characteristics of the conidial stage and the teleomorph, the fungus was identified as Podosphaera ferruginea. This is the first report of goat's-beard powdery mildew caused by Podosphaera ferruginea in Korea.

Effect of Goat Grazing on Surface Water Quality of Alpine Grassland

  • Khan, Ali Sultan;Kim, Jeong-Tae;Kim, Dong-Woo;Park, Ha-Young;Kwon, Chan-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.349-356
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    • 2018
  • The objective of this study was to determine the effect of goat grazing on the surface water quality of the alpine grasslands. Seven sites were selected across the goat farm for water sample collection and analysis. Samples were analyzed for BOD (Biological oxygen demand), total nitrogen, total phosphorous, electrical conductivity and water turbidity. All the above-mentioned parameters remained below the standard limit of Korean government at the end site. Puddles showed higher values, but below standard, as stagnant water has lower physico-chemical properties as of flowing water. The present study clearly showed that goat grazing doesn't affect water quality in grasslands if grazing is according to carrying capacity of grassland and fertilizer application is judicious.

Embryo transfer in Korean Native Black Goat: Embryo recovery and transfer for the production of transgenic goat (한국재래흑염소 수정란의 이식: 형질전환 흑염소 생산을 위한 수정란의 채취와 이식)

  • 신상태
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • pp.64-75
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    • 2000
  • During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period(1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor(hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

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Histological Observation on Development of the Small Intestine of the Korean Native Goat (재래산양(在來山羊)의 소장형성(小腸形成)에 관한 조직학적관찰(組織學的觀察))

  • Kwak, Soo-dong;Kim, Chong-sup
    • Korean Journal of Veterinary Research
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    • v.25 no.1
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    • pp.1-5
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    • 1985
  • The morphological development of the small intestinal tissues of the Korean native goat were observed by light microscopy. Samples were taken from a 60-, 90-, 120-day-old fetus, a newborn goat and a 30-day-old goat. The results were summarized as follows; 1. In the small intestine of 60-day-old fetus, the apexes and sides of villi were covered with a simple columnar epithelium, and intervilous areas and mucosal ridges were still covered with stratified epithelium of two to six cell layers. Mesenchymal tissues formed lamina propria, circular muscle layer and serosa. The numbers of villi per cross section of the small intestine (NVPCS) were 10 to 18. 2. In 90-day-old fetus, intervillous areas and mucosal ridges of the organ were covered with simple columnar epithelium. Goblet cells in epithelium and outer longitudinal muscle layer often appeared. NVPCS were 35 to 60 and Brunner's glands were appeared. 3. In 120-day-old fetus, Brunner's glands of the duodenum and circular connection of outer longitudinal muscle layer were formed, NVPCS were 50 to 87. 4. In newborn goat, Peyer's patches were fully formed and NVPCS were 50 to 87. 5. In 30-day goat, the small intestine was fully matured and NVPCS were 81 to 102.

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Embryo transfer in Korean Native Black Goat;Embryo recovery and transfer for the production of transgenic goat (한국재래흑염소 수정란의 이식;형질전환 흑염소 생산을 위한 수정란의 채취와 이식)

  • Shin, Sang-Tae
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • /
    • pp.64-75
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    • 2000
  • During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period (1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor (hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

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Non-Pathogenic Factors Affecting Somatic Cell Counts of Goat Milk (산양유의 체세포수에 영향을 미치는 비병원성 요인)

  • Kim, Min-Kyung;Choi, A-Ri;Han, Gi-Sung;Jeong, Seok-Geun;Oh, Mi-Hwa;Jang, Ae-Ra;Seol, Kuk-Hwan;Ham, Jun-Sang
    • Journal of Dairy Science and Biotechnology
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    • v.28 no.2
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    • pp.1-5
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    • 2010
  • Somatic cell counts (SCCs) of goat milk can vary widely depending on the counting methods used and non-pathogenic factors; the goat milk industry can be threatened by establishment of a legal standard based on the findings in cow milk. In Korea, SCCs have been excluded from the items that are analyzed under the "Livestock Products Processing and Composition Standards" in accordance with a recent NVRQS Notice amendment. From April to October, SCCs of 150 goat milk samples from 2 farms were analyzed using a Somascope calibrated with standard goat milk samples. Average SCCs of the samples was 598,000/mL, and significant differences were not found between farms and between breeds. SCCs increased from 3 to 8 months after delivery.

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